Search results for: Bacterial recovery
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 606

Search results for: Bacterial recovery

576 Arterial CO2 Pressure Drives Ventilation with a Time Delay during Recovery from an Impulse-like Exercise without Metabolic Acidosis

Authors: R. Afroundeh, T. Arimitsu, R. Yamanaka, C. S. Lian, T. Yunoki, T. Yano, K. Shirakawa

Abstract:

We investigated this hypothesis that arterial CO2 pressure (PaCO2) drives ventilation (V.E) with a time delay duringrecovery from short impulse-like exercise (10 s) with work load of 200 watts. V.E and end tidal CO2 pressure (PETCO2) were measured continuously during rest, warming up, exercise and recovery periods. PaCO2 was predicted (PaCO2 pre) from PETCO2 and tidal volume (VT). PETCO2 and PaCO2 pre peaked at 20 s of recovery. V.E increased and peaked at the end of exercise and then decreased during recovery; however, it peaked again at 30 s of recovery, which was 10 s later than the peak of PaCO2 pre. The relationship between V. E and PaCO2pre was not significant by using data of them obtained at the same time but was significant by using data of V.E obtained 10 s later for data of PaCO2 pre. The results support our hypothesis that PaCO2 drives V.E with a time delay.

Keywords: Arterial CO2 pressure, impulse-like exercise, time delay, ventilation.

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575 IBFO_PSO: Evaluating the Performance of Bio-Inspired Integrated Bacterial Foraging Optimization Algorithm and Particle Swarm Optimization Algorithm in MANET Routing

Authors: K. Geetha, P. Thangaraj, C. Rasi Priya, C. Rajan, S. Geetha

Abstract:

This paper presents the performance of Integrated Bacterial Foraging Optimization and Particle Swarm Optimization (IBFO_PSO) technique in MANET routing. The BFO is a bio-inspired algorithm, which simulates the foraging behavior of bacteria. It is effectively applied in improving the routing performance in MANET. In results, it is proved that the PSO integrated with BFO reduces routing delay, energy consumption and communication overhead.

Keywords: Ant Colony Optimization, Bacterial Foraging Optimization, Hybrid Routing Intelligent Algorithm, Naturally inspired algorithms, Particle Swarm Optimization.

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574 Investigating the Impact of the Laundry and Sterilization Process on the Performance of Reusable Surgical Gowns

Authors: N. Khomarloo, F. Mousazadegan, M. Latifi, N. Hemmatinejad

Abstract:

Recently, the utilization of reusable surgical gowns in order to decrease costs, environmental protection and enhance surgeon’s comfort is considered. One of the concerns in applying this kind of medical protective clothing is reduction of their resistance to bacterial penetration especially in wet state, after repeated laundering and sterilizing process. The purpose of this study is to investigate the effect of the laundering and sterilizing process on the reusable surgical gown’s resistance against bacterial wet penetration. To this end, penetration of Staphylococcus aureus bacteria in wet state after 70 washing and sterilizing cycles was evaluated on the two single-layer and three-layer reusable gowns. The outcomes reveal that up to 20 laundering and sterilizing cycles, protective property of samples improves due to fabric shrinkage, after that because of the fabric’s construction opening, the bacterial penetration increase. However, the three-layer gown presents higher protective performance comparing to the single-layer one.

Keywords: Reusable surgical gown, laundry, sterilization, wet bacterial penetration.

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573 Effect of Oxygen and Micro-Cracking on the Flotation of Low Grade Nickel Sulphide Ore

Authors: Edison Muzenda, Ayo S Afolabi

Abstract:

This study investigated the effect of oxygen and micro-cracking on the flotation of low grade nickel sulphide ore. The ore treated contained serpentine minerals which have a history of being difficult to process efficiently. The use of oxygen as a bubbling gas has been noted to be effective because it increases the pulp potential. The desired effect of micro cracking the ore is that the nickel sulphide minerals will become activated and this activation will render these minerals more susceptible to react with potassium amyl xanthate collectors, resulting in a higher recovery of nickel and hinder the recovery of other undesired minerals contained in the ore. Higher nickel recoveries were obtained when pure oxygen was used as a bubbling gas rather than the conventional air. Microwave cracking favored the recovery of nickel.

Keywords: Flotation, Conventional air, Oven micro-cracking, Recovery.

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572 SeqWord Gene Island Sniffer: a Program to Study the Lateral Genetic Exchange among Bacteria

Authors: Bezuidt O., Lima-Mendez G., Reva O. N.

Abstract:

SeqWord Gene Island Sniffer, a new program for the identification of mobile genetic elements in sequences of bacterial chromosomes is presented. This program is based on the analysis of oligonucleotide usage variations in DNA sequences. 3,518 mobile genetic elements were identified in 637 bacterial genomes and further analyzed by sequence similarity and the functionality of encoded proteins. The results of this study are stored in an open database http://anjie.bi.up.ac.za/geidb/geidbhome. php). The developed computer program and the database provide the information valuable for further investigation of the distribution of mobile genetic elements and virulence factors among bacteria. The program is available for download at www.bi.up.ac.za/SeqWord/sniffer/index.html.

Keywords: mobile genetic elements, virulence, bacterial genomes

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571 Hybrid Recovery of Copper and Silver from PV Ribbon and Ag Finger of EOL Solar Panels

Authors: T. Patcharawit, C. Kansomket, N. Wongnaree, W. Kritsrikan, T. Yingnakorn, S. Khumkoa

Abstract:

Recovery of pure copper and silver from end-of-life photovoltaic (PV) panels was investigated in this paper using an effective hybrid pyro-hydrometallurgical process. In the first step of waste treatment, solar panel waste was first dismantled to obtain a PV sheet to be cut and calcined at 500 °C, to separate out PV ribbon from glass cullet, ash, and volatile while the silicon wafer containing silver finger was collected for recovery. In the second step of metal recovery, copper recovery from PV ribbon was via 1-3 M HCl leaching with SnCl₂ and H₂O₂ additions in order to remove the tin-lead coating on the ribbon. The leached copper band was cleaned and subsequently melted as an anode for the next step of electrorefining. Stainless steel was set as the cathode with CuSO₄ as an electrolyte, and at a potential of 0.2 V, high purity copper of 99.93% was obtained at 96.11% recovery after 24 hours. For silver recovery, the silicon wafer containing silver finger was leached using HNO₃ at 1-4 M in an ultrasonic bath. In the next step of precipitation, silver chloride was then obtained and subsequently reduced by sucrose and NaOH to give silver powder prior to oxy-acetylene melting to finally obtain pure silver metal. The integrated recycling process is considered to be economical, providing effective recovery of high purity metals such as copper and silver while other materials such as aluminum, copper wire, glass cullet can also be recovered to be reused commercially. Compounds such as PbCl₂ and SnO₂ obtained can also be recovered to enter the market.

Keywords: Electrorefining, leaching, calcination, PV ribbon, silver finger, solar panel.

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570 Experimental Investigation of the Impact of Biosurfactants on Residual-Oil Recovery

Authors: S. V. Ukwungwu, A. J. Abbas, G. G. Nasr

Abstract:

The increasing high price of natural gas and oil with attendant increase in energy demand on world markets in recent years has stimulated interest in recovering residual oil saturation across the globe. In order to meet the energy security, efforts have been made in developing new technologies of enhancing the recovery of oil and gas, utilizing techniques like CO2 flooding, water injection, hydraulic fracturing, surfactant flooding etc. Surfactant flooding however optimizes production but poses risk to the environment due to their toxic nature. Amongst proven records that have utilized other type of bacterial in producing biosurfactants for enhancing oil recovery, this research uses a technique to combine biosurfactants that will achieve a scale of EOR through lowering interfacial tension/contact angle. In this study, three biosurfactants were produced from three Bacillus species from freeze dried cultures using sucrose 3 % (w/v) as their carbon source. Two of these produced biosurfactants were screened with the TEMCO Pendant Drop Image Analysis for reduction in IFT and contact angle. Interfacial tension was greatly reduced from 56.95 mN.m-1 to 1.41 mN.m-1 when biosurfactants in cell-free culture (Bacillus licheniformis) were used compared to 4. 83mN.m-1 cell-free culture of Bacillus subtilis. As a result, cell-free culture of (Bacillus licheniformis) changes the wettability of the biosurfactant treatment for contact angle measurement to more water-wet as the angle decreased from 130.75o to 65.17o. The influence of microbial treatment on crushed rock samples was also observed by qualitative wettability experiments. Treated samples with biosurfactants remained in the aqueous phase, indicating a water-wet system. These results could prove that biosurfactants can effectively change the chemistry of the wetting conditions against diverse surfaces, providing a desirable condition for efficient oil transport in this way serving as a mechanism for EOR. The environmental friendly effect of biosurfactants applications for industrial purposes play important advantages over chemically synthesized surfactants, with various possible structures, low toxicity, eco-friendly and biodegradability.

Keywords: Bacillus, biosurfactant, enhanced oil recovery, residual oil, wettability.

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569 Mathematical Modeling of Asphaltene Precipitation: A Review

Authors: Josefina Barnachea Janier, Radzuan B. Razali, Afza Shafie, Brahim Belhaouari Samir

Abstract:

In the Enhanced Oil Recovery (EOR) method, use of Carbon dioxide flooding whereby CO2 is injected into an oil reservoir to increase output when extracting oil resulted significant recovery worldwide. The carbon dioxide function as a pressurizing agent when mixed into the underground crude oil will reduce its viscosity and will enable a rapid oil flow. Despite the CO2’s advantage in the oil recovery, it may result to asphaltene precipitation a problem that will cause the reduction of oil produced from oil wells. In severe cases, asphaltene precipitation can cause costly blockages in oil pipes and machinery. This paper presents reviews of several studies done on mathematical modeling of asphaltene precipitation. The synthesized result from several researches done on this topic can be used as guide in order to better understand asphaltene precipitation. Likewise, this can be used as initial reference for students, and new researchers doing study on asphaltene precipitation.

Keywords: Asphaltene precipitation, crude oil, carbon dioxide flooding, enhanced oil recovery.

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568 A Physics-Based Model for Fast Recovery Diodes with Lifetime Control and Emitter Efficiency Reduction

Authors: Chengjie Wang, Li Yin, Chuanmin Wang

Abstract:

This paper presents a physics-based model for the high-voltage fast recovery diodes. The model provides a good trade-off between reverse recovery time and forward voltage drop realized through a combination of lifetime control and emitter efficiency reduction techniques. The minority carrier lifetime can be extracted from the reverse recovery transient response and forward characteristics. This paper also shows that decreasing the amount of the excess carriers stored in the drift region will result in softer characteristics which can be achieved using a lower doping level. The developed model is verified by experiment and the measurement data agrees well with the model.

Keywords: Emitter efficiency, lifetime control, P-i-N diode, physics-based model

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567 Bio-Surfactant Production and Its Application in Microbial EOR

Authors: A. Rajesh Kanna, G. Suresh Kumar, Sathyanaryana N. Gummadi

Abstract:

There are various sources of energies available worldwide and among them, crude oil plays a vital role. Oil recovery is achieved using conventional primary and secondary recovery methods. In-order to recover the remaining residual oil, technologies like Enhanced Oil Recovery (EOR) are utilized which is also known as tertiary recovery. Among EOR, Microbial enhanced oil recovery (MEOR) is a technique which enables the improvement of oil recovery by injection of bio-surfactant produced by microorganisms. Bio-surfactant can retrieve unrecoverable oil from the cap rock which is held by high capillary force. Bio-surfactant is a surface active agent which can reduce the interfacial tension and reduce viscosity of oil and thereby oil can be recovered to the surface as the mobility of the oil is increased. Research in this area has shown promising results besides the method is echo-friendly and cost effective compared with other EOR techniques. In our research, on laboratory scale we produced bio-surfactant using the strain Pseudomonas putida (MTCC 2467) and injected into designed simple sand packed column which resembles actual petroleum reservoir. The experiment was conducted in order to determine the efficiency of produced bio-surfactant in oil recovery. The column was made of plastic material with 10 cm in length. The diameter was 2.5 cm. The column was packed with fine sand material. Sand was saturated with brine initially followed by oil saturation. Water flooding followed by bio-surfactant injection was done to determine the amount of oil recovered. Further, the injection of bio-surfactant volume was varied and checked how effectively oil recovery can be achieved. A comparative study was also done by injecting Triton X 100 which is one of the chemical surfactant. Since, bio-surfactant reduced surface and interfacial tension oil can be easily recovered from the porous sand packed column.

Keywords: Bio-surfactant, Bacteria, Interfacial tension, Sand column.

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566 Modification and Characterization of Bacterial Cellulose Biopolymer as Proton Conducting Membrane

Authors: C. W. Lin, S.W. Chen

Abstract:

This study describes the preparation of a novel proton conducting membranes based on bacterial cellulose (BC) modified by grafting of 2-acrylamido-2-methyl-1 -propanesulfonic acid (AMPS) through UV-induced graft polymerization. These AMPS-g-BC membranes have been characterized by various techniques including FTIR, SEM and TGA, to find their successful grafting of AMPS on BC, surface morphology and thermal stability, respectively. Physical properties of AMPS-g-BC membranes have been assessed in terms of Lamda value( λ ), ion exchange capacity(IEC) and proton conductivity. The relationship between degree of grafting and AMPS concentration used for grafting has been determined by weight gain method. An optimum proton conductivity equal to 2.89x10-2 S cm-1 and IEC value equal to 1.79 mmol g-1 have been obtained when 20 wt% AMPS concentration is used for grafting (i.e. the corresponding membrane is notated as AMPS20-g-BC).

Keywords: Bacterial cellulose, 2-acrylamido-2-methyl-1-propanesulfonic acid, Proton conducting membrane, Self diffusioncoefficient, Fuel cell

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565 Effects of Polymers and Alkaline on Recovery Improvement from Fractured Models

Authors: Payam Parvasi, Mohammad Hossein Sedaghat, Reza Janamiri, Amir Hatampour

Abstract:

In this work, several ASP solutions were flooded into fractured models initially saturated with heavy oil at a constant flow rate and different geometrical characteristics of fracture. The ASP solutions are constituted from 2 polymers i.e. a synthetic polymer, hydrolyzed polyacrylamide as well as a biopolymer, a surfactant and 2types of alkaline. The results showed that using synthetic hydrolyzed polyacrylamide polymer increases ultimate oil recovery; however, type of alkaline does not play a significant rule on oil recovery. In addition, position of the injection well respect to the fracture system has remarkable effects on ASP flooding. For instance increasing angle of fractures with mean flow direction causes more oil recovery and delays breakthrough time. This work can be accounted as a comprehensive survey on ASP flooding which considers most of effective factors in this chemical EOR method.

Keywords: ASP Flooding, Fractured System, Displacement, Heavy Oil.

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564 Trustworthy Link Failure Recovery Algorithm for Highly Dynamic Mobile Adhoc Networks

Authors: Y. Harold Robinson, M. Rajaram

Abstract:

The Trustworthy link failure recovery algorithm is introduced in this paper, to provide the forwarding continuity even with compound link failures. The ephemeral failures are common in IP networks and it also has some proposals based on local rerouting. To ensure forwarding continuity, we are introducing the compound link failure recovery algorithm, even with compound link failures. For forwarding the information, each packet carries a blacklist, which is a min set of failed links encountered along its path, and the next hop is chosen by excluding the blacklisted links. Our proposed method describes how it can be applied to ensure forwarding to all reachable destinations in case of any two or more link or node failures in the network. After simulating with NS2 contains lot of samples proved that the proposed protocol achieves exceptional concert even under elevated node mobility using Trustworthy link Failure Recovery Algorithm.

Keywords: Wireless Sensor Networks, Predistribution Scheme, Cryptographic Techniques.

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563 Comparative Study of Sub-Critical and Supercritical ORC Applications for Exhaust Waste Heat Recovery

Authors: Buket Boz, Alvaro Diez

Abstract:

Waste heat recovery by means of Organic Rankine Cycle is a promising technology for the recovery of engine exhaust heat. However, it is complex to find out the optimum cycle conditions with appropriate working fluids to match exhaust gas waste heat due to its high temperature. Hence, this paper focuses on comparing sub-critical and supercritical ORC conditions with eight working fluids on a combined diesel engine-ORC system. The model employs two ORC designs, Regenerative-ORC and Pre-Heating-Regenerative-ORC respectively. The thermodynamic calculations rely on the first and second law of thermodynamics, thermal efficiency and exergy destruction factors are the fundamental parameters evaluated. Additionally, in this study, environmental and safety, GWP (Global Warming Potential) and ODP (Ozone Depletion Potential), characteristic of the refrigerants are taken into consideration as evaluation criteria to define the optimal ORC configuration and conditions. Consequently, the studys outcomes reveal that supercritical ORCs with alkane and siloxane are more suitable for high temperature exhaust waste heat recovery in contrast to sub-critical conditions.

Keywords: Internal combustion engine, organic rankine cycle, waste heat recovery, working fluids.

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562 Identification of Cellulose-Hydrolytic Thermophiles Isolated from Sg. Klah Hot Spring Based On 16S rDNA Gene Sequence

Authors: M. J. Norashirene, Y. Zakiah, S. Nurdiana, I. Nur Hilwani, M. H. Siti Khairiyah, M. J. Muhamad Arif

Abstract:

In this study, six bacterial isolates of a slightly thermophilic organism from the Sg. Klah hot spring, Malaysia were successfully isolated and designated as M7T55D1, M7T55D2, M7T55D3, M7T53D1, M7T53D2 and M7T53D3 respectively. The bacterial isolates were screened for their cellulose hydrolytic ability on Carboxymethlycellulose agar medium. The isolated bacterial strains were identified morphologically, biochemically and molecularly with the aid of 16S rDNA sequencing. All of the bacteria showed their optimum growth at a slightly alkaline pH of 7.5 with a temperature of 55°C. All strains were Gram-negative, non-spore forming type, strictly aerobic, catalase-positive and oxidase-positive with the ability to produce thermostable cellulase. Based on BLASTn results, bacterial isolates of M7T55D2 and M7T53D1 gave the highest homology (97%) with similarity to Tepidimonas ignava while isolates M7T55D1, M7T55D3, M7T53D2 and M7T53D3 showed their closest homology (97%-98%) with Tepidimonas thermarum. These cellulolytic thermophiles might have a commercial potential to produce valuable thermostable cellulase.

Keywords: Cellulase, Cellulolytic, Thermophiles, 16S rDNA Gene.

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561 An Investigation of the Cu-Ni Compound Cathode Materials Affecting on Transient Recovery Voltage

Authors: Arunrungrusmi S, Chaokamnerd W, Tanitteerapan T, Mungkung N., Yuji T.

Abstract:

The purpose of this research was to analyze and compare the instability of a contact surface between Copper and Nickel an alloy cathode in vacuum, the different ratio of Copper and Copper were conducted at 1%, 2% and 4% by using the cathode spot model. The transient recovery voltage is predicted. The cathode spot region is recognized as the collisionless space charge sheath connected with singly ionized collisional plasma. It was found that the transient voltage is decreased with increasing the percentage of an amount of Nickel in cathode materials.

Keywords: Vacuum arc, Instability, Low current, Cathode spot, copper, Nickel, Transient Recovery Voltage.

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560 WDM-Based Storage Area Network (SAN) for Disaster Recovery Operations

Authors: Sandeep P. Abhang, Girish V. Chowdhay

Abstract:

This paper proposes a Wavelength Division Multiplexing (WDM) technology based Storage Area Network (SAN) for all type of Disaster recovery operation. It considers recovery when all paths failure in the network as well as the main SAN site failure also the all backup sites failure by the effect of natural disasters such as earthquakes, fires and floods, power outage, and terrorist attacks, as initially SAN were designed to work within distance limited environments[2]. Paper also presents a NEW PATH algorithm when path failure occurs. The simulation result and analysis is presented for the proposed architecture with performance consideration.

Keywords: SAN, WDM, FC, Ring, IP, network load, iSCSI, miles, disaster.

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559 A Bacterial Foraging Optimization Algorithm Applied to the Synthesis of Polyacrylamide Hydrogels

Authors: Florin Leon, Silvia Curteanu

Abstract:

The Bacterial Foraging Optimization (BFO) algorithm is inspired by the behavior of bacteria such as Escherichia coli or Myxococcus xanthus when searching for food, more precisely the chemotaxis behavior. Bacteria perceive chemical gradients in the environment, such as nutrients, and also other individual bacteria, and move toward or in the opposite direction to those signals. The application example considered as a case study consists in establishing the dependency between the reaction yield of hydrogels based on polyacrylamide and the working conditions such as time, temperature, monomer, initiator, crosslinking agent and inclusion polymer concentrations, as well as type of the polymer added. This process is modeled with a neural network which is included in an optimization procedure based on BFO. An experimental study of BFO parameters is performed. The results show that the algorithm is quite robust and can obtain good results for diverse combinations of parameter values.

Keywords: Bacterial foraging optimization, hydrogels, neural networks, modeling.

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558 Water Immersion Recovery for Swimmers in Hot Environments

Authors: Thanura Abeywardena

Abstract:

This study recognized the effectiveness of cold-water immersion recovery post short-term exhaustive exercise. The purpose of this study was to understand if 16-20 °C of cold-water immersion would be beneficial in a tropical environment to achieve an optimal recovery in sprint swim performance in comparison to 10-15 °C of water immersion. Two 100 m-sprint swim performance times were measured along with blood lactate (BLa), heart rate (HR) and rate of perceived exertion (RPE) in a 25 m swimming pool with full body head out horizontal water immersions of 10-15 °C, 16-20 °C and 29-32 °C (pool temperature) for 10 minutes followed by 5 minutes of seated passive rest outside; in between the two swim performances. 10 well-trained adult swimmers (5 male and 5 female) within the top twenty in the Sri Lankan nationals swimming championships in 100m Butterfly and Freestyle in the years 2020 & 2021 volunteered for this study. One-way ANOVA analysis (p < 0.05) suggested performance time, BLa and HR had no significant differences between the three conditions after the second sprint, however RPE was significantly different with p = 0.034 between 10-15 °C and 16-20 °C immersion conditions. The study suggested that the recovery post the two cold-water immersion conditions were similar in terms of performance and physiological factors however the 16-20 °C temperature had a better “feel good” factor post sprint 2. Further study is recommended as there was participant bias with the swimmers not reaching optimal levels in sprint 1. Therefore, they might have been possibly fully recovered before sprint 2 invalidating the physiological effect of recovery.

Keywords: Hydrotherapy, blood lactate, fatigue, recovery, sprint-performance, sprint-swimming.

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557 In Silico Analysis of Quinoxaline Ligand Conformations on 1ZIP: Adenylate Kinase

Authors: Arun Kumar V.A., Keshav Mohan

Abstract:

Adenylate kinase (AK) catalyse the phosphotransferase reaction plays an important role in cellular energy homeostasis. The inhibitors of bacterial AK are useful in the treatment of several bacterial infections. To the novel inhibitors of AK, docking studies performed by using the 3D structure of Bacillus stearothermophilus adenylate kinase from protein data bank (IZIP). 46 Quinoxaline analogues were docked in 1ZIP and selected the highly interacting compounds based on their binding energies, for further studies

Keywords: Kinase, quinoxaline, homeostasis, inhibitor, nucleotide.

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556 The Effect of Plant Growth Promoting Rhizobacteria (PGPR) on Germination, Seedling Growth and Yield of Maize

Authors: A. Gholami, S. Shahsavani, S. Nezarat

Abstract:

The effect of plant growth-promoting rhizobacteria (PGPR) on seed germination, seedling growth and yield of field grown maize were evaluated in three experiments. In these experiments six bacterial strains include P.putida strain R-168, P.fluorescens strain R-93, P.fluorescens DSM 50090, P.putida DSM291, A.lipoferum DSM 1691, A.brasilense DSM 1690 were used. Results of first study showed seed Inoculation significantly enhanced seed germination and seedling vigour of maize. In second experiment, leaf and shoot dry weight and also leaf surface area significantly were increased by bacterial inoculation in both sterile and non-sterile soil. The results showed that inoculation with bacterial treatments had a more stimulating effect on growth and development of plants in nonsterile than sterile soil. In the third experiment, Inoculation of maize seeds with all bacterial strains significantly increased plant height, 100 seed weight, number of seed per ear and leaf area .The results also showed significant increase in ear and shoot dry weight of maize.

Keywords: Azospirillum, biofertilizer, Maize, PGPR, Pseudomonas.

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555 Virulent-GO: Prediction of Virulent Proteins in Bacterial Pathogens Utilizing Gene Ontology Terms

Authors: Chia-Ta Tsai, Wen-Lin Huang, Shinn-Jang Ho, Li-Sun Shu, Shinn-Ying Ho

Abstract:

Prediction of bacterial virulent protein sequences can give assistance to identification and characterization of novel virulence-associated factors and discover drug/vaccine targets against proteins indispensable to pathogenicity. Gene Ontology (GO) annotation which describes functions of genes and gene products as a controlled vocabulary of terms has been shown effectively for a variety of tasks such as gene expression study, GO annotation prediction, protein subcellular localization, etc. In this study, we propose a sequence-based method Virulent-GO by mining informative GO terms as features for predicting bacterial virulent proteins. Each protein in the datasets used by the existing method VirulentPred is annotated by using BLAST to obtain its homologies with known accession numbers for retrieving GO terms. After investigating various popular classifiers using the same five-fold cross-validation scheme, Virulent-GO using the single kind of GO term features with an accuracy of 82.5% is slightly better than VirulentPred with 81.8% using five kinds of sequence-based features. For the evaluation of independent test, Virulent-GO also yields better results (82.0%) than VirulentPred (80.7%). When evaluating single kind of feature with SVM, the GO term feature performs much well, compared with each of the five kinds of features.

Keywords: Bacterial virulence factors, GO terms, prediction, protein sequence.

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554 Design of Controllers to Control Frequency for Distributed Generation

Authors: R. Satish, G. Raja Rao

Abstract:

In this paper a hybrid distributed generation (DG) system connected to isolated load is studied. The DG system consisting of photo voltaic (PV) system, fuel cells, aqua electrolyzer, diesel engine generator and a battery energy storage system. The ambient temperature value of PV is taken as constant to make the output power of PV is directly proportional to the radiation and output power of other DG sources and frequency of the system is controlled by simple integral (I), proportional plus integral (PI), and proportional plus integral and derivative(PID) controllers. A maiden attempt is made to apply a more recent and powerful optimization technique named as bacterial foraging technique for optimization of controllers gains of the proposed hybrid DG system. The system responses with bacterial foraging based controllers are compared with that of classical method. Investigations reveal that bacterial foraging based controllers gives better responses than the classical method and also PID controller is best. Sensitivity analysis is carried out which demonstrates the robustness of the optimized gain values for system loading condition.

Keywords: Aqua electrolyzer, bacterial foraging, battery energy storage system, diesel engine generator, distributed generation, fuel cells, photo voltaic system.

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553 Modelling of Organic Rankine Cycle for Waste Heat Recovery Process in Supercritical Condition

Authors: Jahedul Islam Chowdhury, Bao Kha Nguyen, David Thornhill, Roy Douglas, Stephen Glover

Abstract:

Organic Rankine Cycle (ORC) is the most commonly used method for recovering energy from small sources of heat. The investigation of the ORC in supercritical condition is a new research area as it has a potential to generate high power and thermal efficiency in a waste heat recovery system. This paper presents a steady state ORC model in supercritical condition and its simulations with a real engine’s exhaust data. The key component of ORC, evaporator, is modelled using finite volume method, modelling of all other components of the waste heat recovery system such as pump, expander and condenser are also presented. The aim of this paper is to investigate the effects of mass flow rate and evaporator outlet temperature on the efficiency of the waste heat recovery process. Additionally, the necessity of maintaining an optimum evaporator outlet temperature is also investigated. Simulation results show that modification of mass flow rate is the key to changing the operating temperature at the evaporator outlet.

Keywords: Organic Rankine cycle, supercritical condition, steady state model, waste heat recovery.

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552 Kinetic and Removable of Amoxicillin Using Aliquat336 as a Carrier via a HFSLM

Authors: Teerapon Pirom, Ura Pancharoen

Abstract:

Amoxicillin is an antibiotic which is widely used to treat various infections in both human beings and animals. However, when amoxicillin is released into the environment, it is a major problem. Amoxicillin causes bacterial resistance to these drugs and failure of treatment with antibiotics. Liquid membrane is of great interest as a promising method for the separation and recovery of the target ions from aqueous solutions due to the use of carriers for the transport mechanism, resulting in highly selectivity and rapid transportation of the desired metal ions. The simultaneous processes of extraction and stripping in a single unit operation of liquid membrane system are very interesting. Therefore, it is practical to apply liquid membrane, particularly the HFSLM for industrial applications as HFSLM is proved to be a separation process with lower capital and operating costs, low energy and extractant with long life time, high selectivity and high fluxes compared with solid membranes. It is a simple design amenable to scaling up for industrial applications. The extraction and recovery for (Amoxicillin) through the hollow fiber supported liquid membrane (HFSLM) using aliquat336 as a carrier were explored with the experimental data. The important variables affecting on transport of amoxicillin viz. extractant concentration and operating time were investigated. The highest AMOX- extraction percentages of 85.35 and Amoxicillin stripping of 80.04 were achieved with the best condition at 6 mmol/L [aliquat336] and operating time 100 min. The extraction reaction order (n) and the extraction reaction rate constant (kf) were found to be 1.00 and 0.0344 min-1, respectively.

Keywords: Aliquat336, amoxicillin, HFSLM, kinetic.

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551 Study on Extraction of Niobium Oxide from Columbite–Tantalite Concentrate

Authors: Htet Htike Htwe, Kay Thi Lwin

Abstract:

The principal objective of this study is to be able to extract niobium oxide from columbite-tantalite concentrate of Thayet Kon Area in Nay Phi Taw. It is recovered from columbite-tantalite concentrate which contains 19.29 % Nb2O5.The recovery of niobium oxide from columbite-tantalite concentrate can be divided into three main sections, namely, digestion of the concentrate, recovery from the leached solution and precipitation and calcinations. The concentrate was digested with hydrofluoric acid and sulfuric acid. Of the various parameters that effect acidity and time were studied. In the recovery section solvent extraction process using methyl isobutyl ketone was investigated. Ammonium hydroxide was used as a precipitating agent and the precipitate was later calcined. The percentage of niobium oxide is 74%.

Keywords: Calcination, Digestion, Precipitation, SolventExtraction.

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550 Soft Computing Based Cluster Head Selection in Wireless Sensor Network Using Bacterial Foraging Optimization Algorithm

Authors: A. Rajagopal, S. Somasundaram, B. Sowmya, T. Suguna

Abstract:

Wireless Sensor Networks (WSNs) enable new applications and need non-conventional paradigms for the protocol because of energy and bandwidth constraints, In WSN, sensor node’s life is a critical parameter. Research on life extension is based on Low-Energy Adaptive Clustering Hierarchy (LEACH) scheme, which rotates Cluster Head (CH) among sensor nodes to distribute energy consumption over all network nodes. CH selection in WSN affects network energy efficiency greatly. This study proposes an improved CH selection for efficient data aggregation in sensor networks. This new algorithm is based on Bacterial Foraging Optimization (BFO) incorporated in LEACH.

Keywords: Bacterial Foraging Optimization (BFO), Cluster Head (CH), Data-aggregation protocols, Low-Energy Adaptive Clustering Hierarchy (LEACH).

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549 Evaluation of Bacterial Composition of the Aerosol of Selected Abattoirs in Akure, South Western Nigeria

Authors: Funmilola O. Omoya, Joseph O. Obameso, Titus A. Olukibiti

Abstract:

This study was carried out to reveal the bacterial composition of aerosol in the studied abattoirs. Bacteria isolated were characterized according to microbiological standards. Factors such as temperature and distance were considered as variable in this study. The isolation was carried out at different temperatures such as 27oC, 31oC and 29oC and at various distances of 100meters and 200meters away from the slaughter sites. Result obtained showed that strains of Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Lactobacillus alimentarius and Micrococcus sp. were identified. The total viable counts showed that more microorganisms were present in the morning while the least viable count of 388cfu was recorded in the evening period of this study. This study also showed that more microbial loads were recorded the further the distance is to the slaughter site. Conclusively, the array of bacteria isolated suggests that abattoir sites may be a potential source of pathogenic organisms to commuters if located within residential environment.

Keywords: Abattoir, Aerosol, Bacterial Composition, Environment.

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548 Quality of Bali Beef and Broiler after Immersion in Liquid Smoke on Different Concentrations and Storage Times

Authors: E. Abustam, M. Yusuf, H. M. Ali, M. I. Said, F. N. Yuliati

Abstract:

The aim of this study was to improve the durability and quality of Bali beef (M. Longissimus dorsi) and broiler carcass through the addition of liquid smoke as a natural preservative. This study was using Longissimus dorsi muscle from male Bali beef aged 3 years, broiler breast and thigh aged 40 days. Three types of meat were marinated in liquid smoke with concentrations of 0, 5, and 10% for 30 minutes at the level of 20% of the sample weight (w/w). The samples were storage at 2-5°C for 1 month. This study designed as a factorial experiment 3 x 3 x 4 based on a completely randomized design with 5 replications; the first factor was meat type (beef, chicken breast and chicken thigh); the 2nd factor was liquid smoke concentrations (0, 5, and 10%), and the 3rd factor was storage duration (1, 2, 3, and 4 weeks). Parameters measured were TBA value, total bacterial colonies, water holding capacity (WHC), shear force value both before and after cooking (80°C – 15min.), and cooking loss. The results showed that the type of meat produced WHC, shear force value, cooking loss and TBA differed between the three types of meat. Higher concentration of liquid smoke, the WHC, shear force value, TBA, and total bacterial colonies were decreased; at a concentration of 10% of liquid smoke, the total bacterial colonies decreased by 57.3% from untreated with liquid smoke. Longer storage, the total bacterial colonies and WHC were increased, while the shear force value and cooking loss were decreased. It can be concluded that a 10% concentration of liquid smoke was able to maintain fat oxidation and bacterial growth in Bali beef and chicken breast and thigh.

Keywords: Bali beef, chicken meat, liquid smoke, meat quality.

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547 Investigation into Heterotrophic Activities and Algal Biomass in Surface Flow Stormwater Wetlands

Authors: Wendong Tao

Abstract:

Stormwater wetlands have been mainly designed in an empirical approach for water quality improvement, with little quantitative understanding of the internal microbial processes. This study investigated into heterotrophic bacterial production rate, heterotrophic bacterial mineralization percentage, and algal biomass in hypertrophic and eutrophic surface flow stormwater wetlands. Compared to a nearby wood leachate treatment wetland, the stormwater wetlands had much higher chlorophyll-a concentrations. The eutrophic stormwater wetland had improved water quality, whereas the hypertrophic stormwater wetland had degraded water quality. Heterotrophic bacterial activities in water were limited in the stormwater wetlands due to competition of algal growth for nutrients. The relative contribution of biofilms to the overall heterotrophic activities was higher in the stormwater wetlands than that in the wood leachate treatment wetland.

Keywords: chlorophyll-a, constructed wetland, heterotrophicproduction, mineralization, stormwater

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