@article{(Open Science Index):https://publications.waset.org/pdf/9999483,
	  title     = {Identification of Cellulose-Hydrolytic Thermophiles Isolated from Sg. Klah Hot Spring Based On 16S rDNA Gene Sequence},
	  author    = {M. J. Norashirene and  Y. Zakiah and  S. Nurdiana and  I. Nur Hilwani and  M. H. Siti Khairiyah and  M. J. Muhamad Arif},
	  country	= {},
	  institution	= {},
	  abstract     = {In this study, six bacterial isolates of a slightly
thermophilic organism from the Sg. Klah hot spring, Malaysia were
successfully isolated and designated as M7T55D1, M7T55D2,
M7T55D3, M7T53D1, M7T53D2 and M7T53D3 respectively. The
bacterial isolates were screened for their cellulose hydrolytic ability
on Carboxymethlycellulose agar medium. The isolated bacterial
strains were identified morphologically, biochemically and
molecularly with the aid of 16S rDNA sequencing. All of the bacteria
showed their optimum growth at a slightly alkaline pH of 7.5 with a
temperature of 55°C. All strains were Gram-negative, non-spore
forming type, strictly aerobic, catalase-positive and oxidase-positive
with the ability to produce thermostable cellulase. Based on BLASTn
results, bacterial isolates of M7T55D2 and M7T53D1 gave the
highest homology (97%) with similarity to Tepidimonas ignava while
isolates M7T55D1, M7T55D3, M7T53D2 and M7T53D3 showed
their closest homology (97%-98%) with Tepidimonas thermarum.
These cellulolytic thermophiles might have a commercial potential to
produce valuable thermostable cellulase.
	    journal   = {International Journal of Agricultural and Biosystems Engineering},
	  volume    = {8},
	  number    = {9},
	  year      = {2014},
	  pages     = {1041 - 1044},
	  ee        = {https://publications.waset.org/pdf/9999483},
	  url   	= {https://publications.waset.org/vol/93},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 93, 2014},