Experimental Investigation of the Impact of Biosurfactants on Residual-Oil Recovery

Authors: S. V. Ukwungwu, A. J. Abbas, G. G. Nasr

Abstract:

The increasing high price of natural gas and oil with attendant increase in energy demand on world markets in recent years has stimulated interest in recovering residual oil saturation across the globe. In order to meet the energy security, efforts have been made in developing new technologies of enhancing the recovery of oil and gas, utilizing techniques like CO₂ flooding, water injection, hydraulic fracturing, surfactant flooding etc. Surfactant flooding however optimizes production but poses risk to the environment due to their toxic nature. Amongst proven records that have utilized other type of bacterial in producing biosurfactants for enhancing oil recovery, this research uses a technique to combine biosurfactants that will achieve a scale of EOR through lowering interfacial tension/contact angle. In this study, three biosurfactants were produced from three Bacillus species from freeze dried cultures using sucrose 3 % (w/v) as their carbon source. Two of these produced biosurfactants were screened with the TEMCO Pendant Drop Image Analysis for reduction in IFT and contact angle. Interfacial tension was greatly reduced from 56.95 mN.m^-1 to 1.41 mN.m^-1 when biosurfactants in cell-free culture (Bacillus licheniformis) were used compared to 4. 83mN.m^-1 cell-free culture of Bacillus subtilis. As a result, cell-free culture of (Bacillus licheniformis) changes the wettability of the biosurfactant treatment for contact angle measurement to more water-wet as the angle decreased from 130.75^o to 65.17^o. The influence of microbial treatment on crushed rock samples was also observed by qualitative wettability experiments. Treated samples with biosurfactants remained in the aqueous phase, indicating a water-wet system. These results could prove that biosurfactants can effectively change the chemistry of the wetting conditions against diverse surfaces, providing a desirable condition for efficient oil transport in this way serving as a mechanism for EOR. The environmental friendly effect of biosurfactants applications for industrial purposes play important advantages over chemically synthesized surfactants, with various possible structures, low toxicity, eco-friendly and biodegradability.

Keywords: Bacillus, biosurfactant, enhanced oil recovery, residual oil, wettability.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1112087

References:

[1] Johnson, S., Salehi, M., Eisert, K. & Fox, S. 2009. Using Biosurfactants Produced from Agriculture Process Waste Streams to Improve Oil Recovery in Fractured Carbonate Reservoirs.
[2] De Lima, A. M. & De Souzaa, R. R. 2014. Use of Sugar Cane Vinasse as Substrate for Biosurfactant Production Using Bacillus subtilis PC. Chemical Engineering, 37.
[3] Filho, J. H., Carioca, O. B., Gonzales, R. B., De Lucena, L. & Tavares, C. A. 2012. Biosurfactants: Production and Utilization in Oil Sludge Cleaning. Presented at the SPE EOR Conference at Oil and Gas West Asia held in Muscat, Oman 16 - 18 April 2012. Society of Petroleum Engineers.
[4] Shibulal, B., Al-Bahry, S. N., Al-Wahaibi, Y. M., Elshafie, A. E., Al-Bemani, A. S. & Joshi, S. J. 2014. Microbial Enhanced Heavy Oil Recovery by the Aid of Inhabitant Spore-Forming Bacteria: An Insight Review. The Scientific World Journal, 2014.
[5] Nicholson, W. L., Munakata, N., Horneck, G., Melosh, H. J. & Setlow, P. 2000. Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiology and Molecular Biology Reviews, 64, 548-572.
[6] Lazar, I., Petrisor, I. & Yen, T. 2007. Microbial enhanced oil recovery (MEOR). Petroleum Science and Technology, 25, 1353-1366.
[7] Springham, D. G. 1984. Microbiological methods for the enhancement of oil recovery. Biotechnology and genetic engineering reviews, 1, 187-222.
[8] Bryant, R. S. Potential uses of microorganisms in petroleum recovery technology. Proceedings of the Oklahoma Academy of Science, 1987. 97-104.
[9] Al-Hattali, R. 2012. Microbial Biomass for Improving Sweep Efficiency in Fractured Carbonate Reservoir Using Date Molasses as Renewable Feed Substrate. SPE Annual Technical Conference and Exhibition, 8-10 October, San Antonio, Texas, USA. Society of Petroleum Engineers.
[10] Sen, R. 2008. Biotechnology in petroleum recovery: the microbial EOR. Progress in Energy and Combustion Science, 34, 714-724.
[11] Vazquez-Duhalt, R. & Quintero-Ramirez, R. 2004. Biotechnological approach for development of microbial enhanced oil recovery technique. Petroleum Biotechnology: Developments and Perspectives, 151, 405 -445.
[12] Al-Sulaimani, H., Joshi, S., Al-Wahaibi, Y., Al-Bahry, S., Elshafie, A. & Al-Bemani, A. 2011. Microbial biotechnology for enhancing oil recovery: current developments and future prospects. Biotechnol. Bioinf. Bioeng, 1, 147-158.
[13] Banat, I., Franzetti, A., Gandolfi, I., Bestetti, G., Martinotti, M., Fracchia, L., Smyth, T. & Marchant, R. 2010. Microbial biosurfactants production, applications and future potential. Applied Microbiology and Biotechnology, 87, 427-444.
[14] Hamouda, A. A. & Karoussi, O. 2008. Effect of temperature, wettability and relative permeability on oil recovery from oil-wet chalk. Energies, 1, 19-34.
[15] Mccaffery, F. G. 1972. Measurement of interfacial tensions and contact angles at high temperature and pressure. Journal of Canadian Petroleum Technology, 11.
[16] Wang, W. & Gupta, A. Investigation of the effect of temperature and pressure on wettability using modified pendant drop method. SPE Annual Technical Conference and Exhibition, 1995. Society of Petroleum Engineers.
[17] Dunlap, C., Schisler, D., Price, N. & Vaughn, S. 2011. Cyclic lipopeptide profile of three Bacillus subtilis strains; antagonists of Fusarium head blight. The Journal of Microbiology, 49, 603-609.