Search results for: Prakash S. Kulkarni
17 A Novel Approach for Coin Identification using Eigenvalues of Covariance Matrix, Hough Transform and Raster Scan Algorithms
Authors: J. Prakash, K. Rajesh
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In this paper we present a new method for coin identification. The proposed method adopts a hybrid scheme using Eigenvalues of covariance matrix, Circular Hough Transform (CHT) and Bresenham-s circle algorithm. The statistical and geometrical properties of the small and large Eigenvalues of the covariance matrix of a set of edge pixels over a connected region of support are explored for the purpose of circular object detection. Sparse matrix technique is used to perform CHT. Since sparse matrices squeeze zero elements and contain only a small number of non-zero elements, they provide an advantage of matrix storage space and computational time. Neighborhood suppression scheme is used to find the valid Hough peaks. The accurate position of the circumference pixels is identified using Raster scan algorithm which uses geometrical symmetry property. After finding circular objects, the proposed method uses the texture on the surface of the coins called texton, which are unique properties of coins, refers to the fundamental micro structure in generic natural images. This method has been tested on several real world images including coin and non-coin images. The performance is also evaluated based on the noise withstanding capability.Keywords: Circular Hough Transform, Coin detection, Covariance matrix, Eigenvalues, Raster scan Algorithm, Texton.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 187116 Longitudinal Shear Modulus of Single Aramid, Carbon and Glass Fibres by Torsion Pendulum Tests
Authors: I Prasanna Kumar, Satya Prakash Kushwaha, Preetamkumar Mohite, Sudhir Kamle
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The longitudinal shear moduli of a single aramid, carbon and glass fibres are measured in the present study. A popularly known concept of freely oscillating torsion pendulum has been used to characterize the torsional modulus. A simple freely oscillating torsional pendulum setup is designed with two different types of plastic discs: horizontal and vertical, as the known mass of the pendulum. The time period of the torsional oscillation is measured to determine the torsional rigidity of the fibre. Then the shear modulus of the fibre is calculated from its torsional rigidity. The mean shear modulus of aramid, carbon and glass fibres measured are 6.22±0.09, 18.5±0.91, 38.1±3.55 GPa by horizontal disc pendulum and 6.19±0.13, 18.1±1.34 and 39.5±1.83 GPa by vertical disc pendulum, respectively. The results obtained by both pendulums differed by less than 5% and agreed well with the results reported in literature for these three types of fibres. A detailed uncertainty calculations are carried out for the measurements. It is seen that scatter as well as uncertainty (or error) in the measured shear modulus of these fibres is less than 10%. For aramid fibres the effect of gauge length on the shear modulus value is also studied. It is verified that the scatter in measured shear modulus value increases with gauge length and scatter in fibre diameter.
Keywords: Aramid; Carbon; Glass fibres, Longitudinal shear modulus, Torsion pendulum.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 375315 A Novel VLSI Architecture for Image Compression Model Using Low power Discrete Cosine Transform
Authors: Vijaya Prakash.A.M, K.S.Gurumurthy
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In Image processing the Image compression can improve the performance of the digital systems by reducing the cost and time in image storage and transmission without significant reduction of the Image quality. This paper describes hardware architecture of low complexity Discrete Cosine Transform (DCT) architecture for image compression[6]. In this DCT architecture, common computations are identified and shared to remove redundant computations in DCT matrix operation. Vector processing is a method used for implementation of DCT. This reduction in computational complexity of 2D DCT reduces power consumption. The 2D DCT is performed on 8x8 matrix using two 1-Dimensional Discrete cosine transform blocks and a transposition memory [7]. Inverse discrete cosine transform (IDCT) is performed to obtain the image matrix and reconstruct the original image. The proposed image compression algorithm is comprehended using MATLAB code. The VLSI design of the architecture is implemented Using Verilog HDL. The proposed hardware architecture for image compression employing DCT was synthesized using RTL complier and it was mapped using 180nm standard cells. . The Simulation is done using Modelsim. The simulation results from MATLAB and Verilog HDL are compared. Detailed analysis for power and area was done using RTL compiler from CADENCE. Power consumption of DCT core is reduced to 1.027mW with minimum area[1].Keywords: Discrete Cosine Transform (DCT), Inverse DiscreteCosine Transform (IDCT), Joint Photographic Expert Group (JPEG), Low Power Design, Very Large Scale Integration (VLSI) .
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 313214 Comparative Dynamic Performance of Load Frequency Control of Nonlinear Interconnected Hydro-Thermal System Using Intelligent Techniques
Authors: Banaja Mohanty, Prakash Kumar Hota
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This paper demonstrates dynamic performance evaluation of load frequency control (LFC) with different intelligent techniques. All non-linearities and physical constraints have been considered in simulation studies such as governor dead band (GDB), generation rate constraint (GRC) and boiler dynamics. The conventional integral time absolute error has been considered as objective function. The design problem is formulated as an optimisation problem and particle swarm optimisation (PSO), bacterial foraging optimisation algorithm (BFOA) and differential evolution (DE) are employed to search optimal controller parameters. The superiority of the proposed approach has been shown by comparing the results with published fuzzy logic control (FLC) for the same interconnected power system. The comparison is done using various performance measures like overshoot, undershoot, settling time and standard error criteria of frequency and tie-line power deviation following a step load perturbation (SLP). It is noticed that, the dynamic performance of proposed controller is better than FLC. Further, robustness analysis is carried out by varying the time constants of speed governor, turbine, tie-line power in the range of +40% to -40% to demonstrate the robustness of the proposed DE optimized PID controller.Keywords: Automatic generation control, governor dead band, generation rate constraint, differential evolution.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 105413 Analysis of Surface Hardness, Surface Roughness, and Near Surface Microstructure of AISI 4140 Steel Worked with Turn-Assisted Deep Cold Rolling Process
Authors: P. R. Prabhu, S. M. Kulkarni, S. S. Sharma, K. Jagannath, Achutha Kini U.
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In the present study, response surface methodology has been used to optimize turn-assisted deep cold rolling process of AISI 4140 steel. A regression model is developed to predict surface hardness and surface roughness using response surface methodology and central composite design. In the development of predictive model, deep cold rolling force, ball diameter, initial roughness of the workpiece, and number of tool passes are considered as model variables. The rolling force and the ball diameter are the significant factors on the surface hardness and ball diameter and numbers of tool passes are found to be significant for surface roughness. The predicted surface hardness and surface roughness values and the subsequent verification experiments under the optimal operating conditions confirmed the validity of the predicted model. The absolute average error between the experimental and predicted values at the optimal combination of parameter settings for surface hardness and surface roughness is calculated as 0.16% and 1.58% respectively. Using the optimal processing parameters, the surface hardness is improved from 225 to 306 HV, which resulted in an increase in the near surface hardness by about 36% and the surface roughness is improved from 4.84µm to 0.252 µm, which resulted in decrease in the surface roughness by about 95%. The depth of compression is found to be more than 300µm from the microstructure analysis and this is in correlation with the results obtained from the microhardness measurements. Taylor hobson talysurf tester, micro vickers hardness tester, optical microscopy and X-ray diffractometer are used to characterize the modified surface layer.
Keywords: Surface hardness, response surface methodology, microstructure, central composite design, deep cold rolling, surface roughness.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 179112 Fatigue Crack Growth Behavior in Dissimilar Metal Weldment of Stainless Steel and Carbon Steel
Authors: K. Krishnaprasad, Raghu V. Prakash
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Constant amplitude fatigue crack growth (FCG) tests were performed on dissimilar metal welded plates of Type 316L Stainless Steel (SS) and IS 2062 Grade A Carbon steel (CS). The plates were welded by TIG welding using SS E309 as electrode. FCG tests were carried on the Side Edge Notch Tension (SENT) specimens of 5 mm thickness, with crack initiator (notch) at base metal region (BM), weld metal region (WM) and heat affected zones (HAZ). The tests were performed at a test frequency of 10 Hz and at load ratios (R) of 0.1 & 0.6. FCG rate was found to increase with stress ratio for weld metals and base metals, where as in case of HAZ, FCG rates were almost equal at high ΔK. FCG rate of HAZ of stainless steel was found to be lowest at low and high ΔK. At intermediate ΔK, WM showed the lowest FCG rate. CS showed higher crack growth rate at all ΔK. However, the scatter band of data was found to be narrow. Fracture toughness (Kc) was found to vary in different locations of weldments. Kc was found lowest for the weldment and highest for HAZ of stainless steel. A novel method of characterizing the FCG behavior using an Infrared thermography (IRT) camera was attempted. By monitoring the temperature rise at the fast moving crack tip region, the amount of plastic deformation was estimated.Keywords: Dissimilar metal weld, Fatigue Crack Growth, fracture toughness, Infrared thermography.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 287811 Dynamic Risk Identification Using Fuzzy Failure Mode Effect Analysis in Fabric Process Industries: A Research Article as Management Perspective
Authors: A. Sivakumar, S. S. Darun Prakash, P. Navaneethakrishnan
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In and around Erode District, it is estimated that more than 1250 chemical and allied textile processing fabric industries are affected, partially closed and shut off for various reasons such as poor management, poor supplier performance, lack of planning for productivity, fluctuation of output, poor investment, waste analysis, labor problems, capital/labor ratio, accumulation of stocks, poor maintenance of resources, deficiencies in the quality of fabric, low capacity utilization, age of plant and equipment, high investment and input but low throughput, poor research and development, lack of energy, workers’ fear of loss of jobs, work force mix and work ethic. The main objective of this work is to analyze the existing conditions in textile fabric sector, validate the break even of Total Productivity (TP), analyze, design and implement fuzzy sets and mathematical programming for improvement of productivity and quality dimensions in the fabric processing industry. It needs to be compatible with the reality of textile and fabric processing industries. The highly risk events from productivity and quality dimension were found by fuzzy systems and results are wrapped up among the textile fabric processing industry.
Keywords: Break Even Point, Fuzzy Crisp Data, Fuzzy Sets, Productivity, Productivity Cycle, Total Productive Maintenance.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 189010 Simulation and Analysis of Control System for a Solar Desalination System
Authors: R. Prakash, B. Meenakshipriya, R. Kumaravelan
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Fresh water is one of the resources which is getting depleted day by day. A wise method to address this issue is by the application of renewable energy-sun irradiation and by means of decentralized, cheap, energetically self-sufficient, robust and simple to operate plants, distillates can be obtained from sea, river or even sewage. Solar desalination is a technique used to desalinate water using solar energy. The present work deals with the comprehensive design and simulation of solar tracking system using LabVIEW, temperature and mass flow rate control of the solar desalination plant using LabVIEW and also analysis of single phase inverter circuit with LC filters for solar pumping system in MATLAB. The main objective of this work is to improve the performance of solar desalination system using automatic tracking system, output control using temperature and mass flow rate control system and also to reduce the harmonic distortion in the solar pumping system by means of LC filters. The simulation of single phase inverter was carried out using MATLAB and the output waveforms were analyzed. Simulations were performed for optimum output temperature control, which in turn controls the mass flow rate of water in the thermal collectors. Solar tracking system was accomplished using LABVIEW and was tested successfully. The thermal collectors are tracked in accordance with the sun’s irradiance levels, thereby increasing the efficiency of the thermal collectors.Keywords: Desalination, Electro dialysis, LabVIEW, MATLAB, PWM inverter, Reverse osmosis.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 23879 Hi-Fi Traffic Clearance Technique for Life Saving Vehicles using Differential GPS System
Authors: N. Yuvaraj, V. B. Prakash, D. Venkatraj
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This paper may be considered as combination of both pervasive computing and Differential GPS (global positioning satellite) which relates to control automatic traffic signals in such a way as to pre-empt normal signal operation and permit lifesaving vehicles. Before knowing the arrival of the lifesaving vehicles from the signal there is a chance of clearing the traffic. Traffic signal preemption system includes a vehicle equipped with onboard computer system capable of capturing diagnostic information and estimated location of the lifesaving vehicle using the information provided by GPS receiver connected to the onboard computer system and transmitting the information-s using a wireless transmitter via a wireless network. The fleet management system connected to a wireless receiver is capable of receiving the information transmitted by the lifesaving vehicle .A computer is also located at the intersection uses corrected vehicle position, speed & direction measurements, in conjunction with previously recorded data defining approach routes to the intersection, to determine the optimum time to switch a traffic light controller to preemption mode so that lifesaving vehicles can pass safely. In case when the ambulance need to take a “U" turn in a heavy traffic area we suggest a solution. Now we are going to make use of computerized median which uses LINKED BLOCKS (removable) to solve the above problem.Keywords: Ubiquitous computing, differential GPS, fleet management system, wireless transmitter and receiver computerized median i.e. linked blocks (removable).
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19828 Kinetic, Thermodynamic and Process Modeling of Synthesis of UV Curable Glyceryl and Neopentyl Glycol Acrylates
Authors: R. D. Kulkarni, Mayur Chaudhari, S. Mishra
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Curing of paints by exposure to UV radiations is emerging as one of the best film forming technique as an alternative to traditional solvent borne oxidative and thermal curing coatings. The composition and chemistry of UV curable coatings and role of multifunctional and monofunctional monomers, oligomers, and photoinitiators have been discussed. The limitations imposed by thermodynamic equilibrium and tendency for acrylic double bond polymerizations during synthesis of multifunctional acrylates have been presented. Aim of present investigation was thus to explore the reaction variables associated with synthesis of multifunctional acrylates. Zirconium oxychloride was evaluated as catalyst against regular acid functional catalyst. The catalyzed synthesis of glyceryl acrylate and neopentyl glycol acrylate was conducted by variation of following reaction parameters: two different reactant molar ratios- 1:4 and 1:6; catalyst usage in % by moles on polyol- 2.5, 5.0 and 7.5 and two different reaction temperatures- 45 and 75 0C. The reaction was monitored by determination of acid value and hydroxy value at regular intervals, besides TLC, HPLC, and FTIR analysis of intermediates and products. On the basis of determination of reaction progress over 1-60 hrs, the esterification reaction was observed to follow 2nd order kinetics with rate constant varying from 1*10-4 to 7*10-4. The thermal and catalytic components of second order rate constant and energy of activation were also determined. Uses of these kinetic and thermodynamic parameters in design of reactor for manufacture of multifunctional acrylate ester have been presented. The synthesized multifunctional acrylates were used to formulate and apply UV curable clear coat followed by determination of curing characteristics and mechanical properties of cured film. The overall curing rates less than 05 min. were easily attained indicating economical viability of radiation curable system due to faster production schedulesKeywords: glyceryl acrylate, neopentyl glycol acrylate, kinetic modeling, zirconium oxychloride.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 22907 Identification of Critical Success Factors in Non-Formal Service Sector Using Delphi Technique
Authors: Amol A. Talankar, Prakash Verma, Nitin Seth
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The purpose of this study is to identify the critical success factors (CSFs) for the effective implementation of Six Sigma in non-formal service Sectors.
Based on the survey of literature, the critical success factors (CSFs) for Six Sigma have been identified and are assessed for their importance in Non-formal service sector using Delphi Technique. These selected CSFs were put forth to the panel of expert to cluster them and prepare cognitive map to establish their relationship.
All the critical success factors examined and obtained from the review of literature have been assessed for their importance with respect to their contribution to Six Sigma effectiveness in non formal service sector.
The study is limited to the non-formal service sectors involved in the organization of religious festival only. However, the similar exercise can be conducted for broader sample of other non-formal service sectors like temple/ashram management, religious tours management etc.
The research suggests an approach to identify CSFs of Six Sigma for Non-formal service sector. All the CSFs of the formal service sector will not be applicable to Non-formal services, hence opinion of experts was sought to add or delete the CSFs. In the first round of Delphi, the panel of experts has suggested, two new CSFs-“competitive benchmarking (F19) and resident’s involvement (F28)”, which were added for assessment in the next round of Delphi. One of the CSFs-“fulltime six sigma personnel (F15)” has been omitted in proposed clusters of CSFs for non-formal organization, as it is practically impossible to deploy full time trained Six Sigma recruits.
Keywords: Critical success factors (CSFs), Quality assurance, non-formal service sectors, Six Sigma.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 24396 Vibration Characteristics of Functionally Graded Material Skew Plate in Thermal Environment
Authors: Gulshan Taj M. N. A., Anupam Chakrabarti, Vipul Prakash
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In the present investigation, free vibration of functionally graded material (FGM) skew plates under thermal environment is studied. Kinematics equations are based on the Reddy’s higher order shear deformation theory and a nine noded isoparametric Lagrangian element is adopted to mesh the plate geometry. The issue of C1 continuity requirement related to the assumed displacement field has been circumvented effectively to develop C0 finite element formulation. Effective mechanical properties of the constituents of the plate are considered to be as position and temperature dependent and assumed to vary in the thickness direction according to a simple power law distribution. The displacement components of a rectangular plate are mapped into skew plate geometry by means of suitable transformation rule. One dimensional Fourier heat conduction equation is used to ascertain the temperature profile of the plate along thickness direction. Influence of different parameters such as volume fraction index, boundary condition, aspect ratio, thickness ratio and temperature field on frequency parameter of the FGM skew plate is demonstrated by performing various examples and the related findings are discussed briefly. New results are generated for vibration of the FGM skew plate under thermal environment, for the first time, which may be implemented in the future research involving similar kind of problems.
Keywords: Functionally graded material, finite element method, higher order shear deformation theory, skew plate, thermal vibration.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 35965 Very Large Scale Integration Architecture of Finite Impulse Response Filter Implementation Using Retiming Technique
Authors: S. Jalaja, A. M. Vijaya Prakash
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Recursive combination of an algorithm based on Karatsuba multiplication is exploited to design a generalized transpose and parallel Finite Impulse Response (FIR) Filter. Mid-range Karatsuba multiplication and Carry Save adder based on Karatsuba multiplication reduce time complexity for higher order multiplication implemented up to n-bit. As a result, we design modified N-tap Transpose and Parallel Symmetric FIR Filter Structure using Karatsuba algorithm. The mathematical formulation of the FFA Filter is derived. The proposed architecture involves significantly less area delay product (APD) then the existing block implementation. By adopting retiming technique, hardware cost is reduced further. The filter architecture is designed by using 90 nm technology library and is implemented by using cadence EDA Tool. The synthesized result shows better performance for different word length and block size. The design achieves switching activity reduction and low power consumption by applying with and without retiming for different combination of the circuit. The proposed structure achieves more than a half of the power reduction by adopting with and without retiming techniques compared to the earlier design structure. As a proof of the concept for block size 16 and filter length 64 for CKA method, it achieves a 51% as well as 70% less power by applying retiming technique, and for CSA method it achieves a 57% as well as 77% less power by applying retiming technique compared to the previously proposed design.Keywords: Carry save adder Karatsuba multiplication, mid-range Karatsuba multiplication, modified FFA, transposed filter, retiming.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 8994 Elliptical Features Extraction Using Eigen Values of Covariance Matrices, Hough Transform and Raster Scan Algorithms
Authors: J. Prakash, K. Rajesh
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In this paper, we introduce a new method for elliptical object identification. The proposed method adopts a hybrid scheme which consists of Eigen values of covariance matrices, Circular Hough transform and Bresenham-s raster scan algorithms. In this approach we use the fact that the large Eigen values and small Eigen values of covariance matrices are associated with the major and minor axial lengths of the ellipse. The centre location of the ellipse can be identified using circular Hough transform (CHT). Sparse matrix technique is used to perform CHT. Since sparse matrices squeeze zero elements and contain a small number of nonzero elements they provide an advantage of matrix storage space and computational time. Neighborhood suppression scheme is used to find the valid Hough peaks. The accurate position of circumference pixels is identified using raster scan algorithm which uses the geometrical symmetry property. This method does not require the evaluation of tangents or curvature of edge contours, which are generally very sensitive to noise working conditions. The proposed method has the advantages of small storage, high speed and accuracy in identifying the feature. The new method has been tested on both synthetic and real images. Several experiments have been conducted on various images with considerable background noise to reveal the efficacy and robustness. Experimental results about the accuracy of the proposed method, comparisons with Hough transform and its variants and other tangential based methods are reported.Keywords: Circular Hough transform, covariance matrix, Eigen values, ellipse detection, raster scan algorithm.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 26323 Learners’ Violent Behaviour and Drug Abuse as Major Causes of Tobephobia in Schools
Authors: Prakash Singh
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Many schools throughout the world are facing constant pressure to cope with the violence and drug abuse of learners who show little or no respect for acceptable and desirable social norms. These delinquent learners tend to harbour feelings of being beyond reproach because they strongly believe that it is well within their rights to engage in violent and destructive behaviour. Knives, guns, and other weapons appear to be more readily used by them on the school premises than before. It is known that learners smoke, drink alcohol, and use drugs during school hours, hence, their ability to concentrate, work, and learn, is affected. They become violent and display disruptive behaviour in their classrooms as well as on the school premises, and this atrocious behaviour makes it possible for drug dealers and gangsters to gain access onto the school premises. The primary purpose of this exploratory quantitative study was therefore to establish how tobephobia (TBP), caused by school violence and drug abuse, affects teaching and learning in schools. The findings of this study affirmed that poor discipline resulted in producing poor quality education. Most of the teachers in this study agreed that educating learners who consumed alcohol and other drugs on the school premises resulted in them suffering from TBP. These learners are frequently abusive and disrespectful, and resort to violence to seek attention. As a result, teachers feel extremely demotivated and suffer from high levels of anxiety and stress. The word TBP will surely be regarded as a blessing by many teachers throughout the world because finally, there is a word that will make people sit up and listen to their problems that cause real fear and anxiety in schools.Keywords: Aims and objectives of quality education, Debilitating effects of tobephobia, Fear of failure associated with education, learners’ violent behaviour and drug abuse.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 12602 Design and Development of On-Line, On-Site, In-Situ Induction Motor Performance Analyser
Authors: G. S. Ayyappan, Srinivas Kota, Jaffer R. C. Sheriff, C. Prakash Chandra Joshua
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In the present scenario of energy crises, energy conservation in the electrical machines is very important in the industries. In order to conserve energy, one needs to monitor the performance of an induction motor on-site and in-situ. The instruments available for this purpose are very meager and very expensive. This paper deals with the design and development of induction motor performance analyser on-line, on-site, and in-situ. The system measures only few electrical input parameters like input voltage, line current, power factor, frequency, powers, and motor shaft speed. These measured data are coupled to name plate details and compute the operating efficiency of induction motor. This system employs the method of computing motor losses with the help of equivalent circuit parameters. The equivalent circuit parameters of the concerned motor are estimated using the developed algorithm at any load conditions and stored in the system memory. The developed instrument is a reliable, accurate, compact, rugged, and cost-effective one. This portable instrument could be used as a handy tool to study the performance of both slip ring and cage induction motors. During the analysis, the data can be stored in SD Memory card and one can perform various analyses like load vs. efficiency, torque vs. speed characteristics, etc. With the help of the developed instrument, one can operate the motor around its Best Operating Point (BOP). Continuous monitoring of the motor efficiency could lead to Life Cycle Assessment (LCA) of motors. LCA helps in taking decisions on motor replacement or retaining or refurbishment.
Keywords: Energy conservation, equivalent circuit parameters, induction motor efficiency, life cycle assessment, motor performance analysis.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 9421 Screening and Evaluation of in vivo and in vitro Generated Insulin Plant (Vernonia divergens) for Antimicrobial and Anticancer Activities
Authors: Santosh Kumar, Anand Prakash, Kanak Sinha, Anita K Verma
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Vernonia divergens Benth., commonly known as “Insulin Plant” (Fam: Asteraceae) is a potent sugar killer. Locally the leaves of the plant, boiled in water are successfully administered to a large number of diabetic patients. The present study evaluates the putative anti-diabetic ingredients, isolated from the in vivo and in vitro grown plantlets of V. divergens for their antimicrobial and anticancer activities. Sterilized explants of nodal segments were cultured on MS (Musashige and Skoog, 1962) medium in presence of different combinations of hormones. Multiple shoots along with bunch of roots were regenerated at 1mg l-1 BAP and 0.5 mg l-1 NAA. Micro-plantlets were separated and sub-cultured on the double strength (2X) of the above combination of hormones leading to increased length of roots and shoots. These plantlets were successfully transferred to soil and survived well in nature. The ethanol extract of plantlets from both in vivo & in vitro sources were prepared in soxhlet extractor and then concentrated to dryness under reduced pressure in rotary evaporator. Thus obtainedconcentrated extracts showed significant inhibitory activity against gram negative bacteria like Escherichia coli and Pseudomonas aeruginosa but no inhibition was found against gram positive bacteria. Further, these ethanol extracts were screened for in vitro percentage cytotoxicity at different time periods (24 h, 48 h and 72 h) of different dilutions. The in vivo plant extract inhibited the growth of EAC mouse cell lines in the range of 65, 66, 78, and 88% at 100, 50, 25 & 12.5μg mL-1 but at 72 h of treatment. In case of the extract of in vitro origin, the inhibition was found against EAC cell lines even at 48h. During spectrophotometric scanning, the extracts exhibited different maxima (ʎ) - four peaks in in vitro extracts as against single in in vivo preparation suggesting the possible change in the nature of ingredients during micropropagation through tissue culture techniques.Keywords: Anti-cancer, Anti-microbial, EAC mouse cell, Tissue culture, Vernonia divergens.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2352