Search results for: Microstrip Lines
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 395

Search results for: Microstrip Lines

185 Detecting the Capacity Reserve in an Overhead Line

Authors: S. Berjozkina, A. Sauhats, V. Bargels, E. Vanzovichs

Abstract:

There are various solutions for improving existing overhead line systems with the general purpose of increasing their limited capacity. The capacity reserve of the existing overhead lines is an important problem that must be considered from different aspects. The paper contains a comparative analysis of the mechanical and thermal limitations of an existing overhead line based on certain calculation conditions characterizing the examined variants. The methodology of the proposed estimation of the permissible conductor temperature and maximum load current is described in detail. The transmission line model consists of specific information of an existing overhead line of the Latvian power network. The main purpose of the simulation tasks is to find an additional capacity reserve by using accurate mathematical models. The results of the obtained data are presented.

Keywords: capacity of an overhead line, mechanical conditions, permissible conductor temperature, thermal conditions.

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184 Detection of Airborne Bacteria and Mildew in the Shanghai Metro System

Authors: Feng Zhou, Yuyan Wang

Abstract:

This study aimed to detect and to identify the main strains of airborne microorganisms present in the Shanghai Metro system. Samples were collected using agar plates exposed to the air and microorganisms were identified using catalase, plasma coagulase and hymolytic analysis. The results show that the concentration of mildew present within a newly opened metro line was significantly higher than for other lines. Differences among underground and elevated stations can be attributed to differences in passenger flow and the environment surrounding the stations. Additionally, the investigation indicated that bacteria reached maximum levels at different times on weekdays and weekends. The bacteria in the Metro stations were identified as primarily Gram positive, consisting mainly of coagulase-negative staphylococcus strains (CNS).

Keywords: Bacteria, environment, Metro system, mildew, passenger flow

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183 An Approach in the Improvement of the Reliability of Impedance Relay

Authors: D. Ouahdi, R. Ladjeroud, I. Habi

Abstract:

The distance protection mainly the impedance relay which is considered as the main protection for transmission lines can be subjected to impedance measurement error which is, mainly, due to the fault resistance and to the power fluctuation. Thus, the impedance relay may not operate for a short circuit at the far end of the protected line (case of the under reach) or operates for a fault beyond its protected zone (case of overreach). In this paper, an approach to fault detection by a distance protection, which distinguishes between the faulty conditions and the effect of overload operation mode, has been developed. This approach is based on the symmetrical components; mainly the negative sequence, and it is taking into account both the effect of fault resistance and the overload situation which both have an effect upon the reliability of the protection in terms of dependability for the former and security for the latter.

Keywords: Distance Protection, Fault Detection, negative sequence, overload, Transmission line.

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182 Liquid-Liquid Equilibria for Ternary Mixtures of (Water + Carboxylic Acid+ MIBK), Experimental, Simulation, and Optimization

Authors: D. Laiadi, A. Hasseine, A. Merzougui

Abstract:

In this work, Experimental tie-line results and solubility (binodal) curves were obtained for the ternary systems (water + acetic acid + methyl isobutyl ketone (MIBK)), (water + lactic acid+ methyl isobutyl ketone) at T = 294.15K and atmospheric pressure. The consistency of the values of the experimental tie-lines was determined through the Othmer-Tobias and Hands correlations. For the extraction effectiveness of solvents, the distribution and selectivity curves were plotted. In addition, these experimental tieline data were also correlated with NRTL model. The interaction parameters for the NRTL model were retrieved from the obtained experimental results by means of a combination of the homotopy method and the genetic algorithms.

Keywords: Liquid-liquid equilibria, homotopy methods, carboxylic acid, NRTL.

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181 Effects of Winter and Spring Sowing on Yield Components of Safflower Genotypes

Authors: Rahim Ada

Abstract:

The research was conducted with three replications as “Randomized Block Design” in Konya-Turkey ecological conditions. In the study, 16 of promising safflower lines (A8, E1, F4, F6, G16, H14, I1), and 1 cultivar (Dinçer) were evaluated in 2008-09 growing season. Some of the yield components such as plant height (cm), first branch height (cm), number of branches per plant, 1000 seed weight (g), seed yield (kg ha-1), oil content (%), oil yield (kg ha-1) were determined. Winter sowing showed higher values than spring sowing. The highest values were taken from Dinçer for plant height (86.7 cm), E1 (37.5 cm) for first branch height, F6 for number of branch (11.6 per plant), I1 for number of head (24.9 per plant), A8 for 1000 seed weight (51.75 g), Dinçer for seed yield (2927.1 kg ha-1), oil content (28.79 %) and also for oil yield (87.44 kg ha-1) respectively.

Keywords: Oil yield, Safflower, Sowing time, Yield components

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180 A MATLAB Simulink Library for Transient Flow Simulation of Gas Networks

Authors: M. Behbahani-Nejad, A. Bagheri

Abstract:

An efficient transient flow simulation for gas pipelines and networks is presented. The proposed transient flow simulation is based on the transfer function models and MATLABSimulink. The equivalent transfer functions of the nonlinear governing equations are derived for different types of the boundary conditions. Next, a MATLAB-Simulink library is developed and proposed considering any boundary condition type. To verify the accuracy and the computational efficiency of the proposed simulation, the results obtained are compared with those of the conventional finite difference schemes (such as TVD, method of lines, and other finite difference implicit and explicit schemes). The effects of the flow inertia and the pipeline inclination are incorporated in this simulation. It is shown that the proposed simulation has a sufficient accuracy and it is computationally more efficient than the other methods.

Keywords: Gas network, MATLAB-Simulink, transfer functions, transient flow.

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179 Model of Multi-Criteria Evaluation for Railway Lines

Authors: Juraj Camaj, Martin Kendra, Jaroslav Masek

Abstract:

The paper is focused to the evaluation railway tracks in the Slovakia by using Multi-Criteria method. Evaluation of railway tracks has important impacts for the assessment of investment in technical equipment. Evaluation of railway tracks also has an important impact for the allocation of marshalling yards. Marshalling yards are in transport model as centers for the operation assigned catchment area. This model is one of the effective ways to meet the development strategy of the European Community's railways. By applying this model in practice, a transport company can guarantee a higher quality of service and then expect an increase in performance. The model is also applicable to other rail networks. This model supplements a theoretical problem of train formation problem of new ways of looking at evaluation of factors affecting the organization of wagon flows.

Keywords: Railway track, multi-criteria methods, evaluation, transportation model.

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178 A Comparative Study of Image Segmentation Algorithms

Authors: Mehdi Hosseinzadeh, Parisa Khoshvaght

Abstract:

In some applications, such as image recognition or compression, segmentation refers to the process of partitioning a digital image into multiple segments. Image segmentation is typically used to locate objects and boundaries (lines, curves, etc.) in images. Image segmentation is to classify or cluster an image into several parts (regions) according to the feature of image, for example, the pixel value or the frequency response. More precisely, image segmentation is the process of assigning a label to every pixel in an image such that pixels with the same label share certain visual characteristics. The result of image segmentation is a set of segments that collectively cover the entire image, or a set of contours extracted from the image. Several image segmentation algorithms were proposed to segment an image before recognition or compression. Up to now, many image segmentation algorithms exist and be extensively applied in science and daily life. According to their segmentation method, we can approximately categorize them into region-based segmentation, data clustering, and edge-base segmentation. In this paper, we give a study of several popular image segmentation algorithms that are available.

Keywords: Image Segmentation, hierarchical segmentation, partitional segmentation, density estimation.

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177 Design of the Production Line Based On RFID through 3D Modeling

Authors: Aliakbar Akbari, Majid Hashemipour, Shiva Mirshahi

Abstract:

Radio-frequency identification has entered as a beneficial means with conforming GS1 standards to provide the best solutions in the manufacturing area. It competes with other automated identification technologies e.g. barcodes and smart cards with regard to high speed scanning, reliability and accuracy as well. The purpose of this study is to improve production line-s performance by implementing RFID system in the manufacturing area on the basis of radio-frequency identification (RFID) system by 3D modeling in the program Cinema 4D R13 which provides obvious graphical scenes for users to portray their applications. Finally, with regard to improving system performance, it shows how RFID appears as a well-suited technology in a comparison of the barcode scanner to handle different kinds of raw materials in the production line base on logical process.

Keywords: Radio Frequency Identification, Manufacturing and Production Lines, 3D modeling

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176 Study of the Flow Structure in a Penstock in Unsteady Regime

Authors: F. Nkontchou Ngongang, M. Tchawe Tchawe, B. Djeumako, B. Kenmeugne

Abstract:

In this work, the flow structure in the Songloulou dam, is visualized in a time interval to observe the different fluid layers in our structure. Firstly, the three-dimensional modelling of the penstock is carried out in the software Gambit, followed by calculations in Fluent that proceeds introduction of boundary conditions. After calculation, we identified four periods corresponding to four regimes. In the first, spanning from 0.00 to 1.50s, we have the non-developed hydraulically rough turbulent regime, characterized by abrupt variations with modifications of the velocity fields. The second extends from 1.50 to 3.50s, where we have the transition regime characterized by slight variations and modifications of the velocity fields but with a great difference of the values of the current lines. From 3.50 to 5.00s, we encounter the third, which is the fully developed turbulent hydraulically rough regime, characterized by fields that vary no more, but have minute differences in the streamlines. The last period is from 5.00s and more, where we have a flow that is almost stationary, hence there are no changes in the fields.

Keywords: Unsteady flow, penstock, friction coefficient, hydroelectric dam.

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175 Developing Intellectual Capital to Advance Innovation and Entrepreneurial Capacity and Sustain Knowledge Economy

Authors: Hamid Alalwany, Nabeel A. Koshak, Mohammad K. Ibrahim

Abstract:

Both knowledge economy and sustainable development are considered key dimensions in the policy action lines of many developed and developing countries. In this context, universities and other higher education institutes have a vital role in developing and sustaining wellbeing communities.

In this paper, the authors’ aim is to address the links between the concepts of innovation and entrepreneurial capacity and knowledge economy, and to utilize the approach of intellectual capital development in building a sustainable knowledge economy.

The paper will contribute to two discourses:

  1. Developing a common understanding of the intersection aspects between the three concepts: Knowledge economy, Innovation and entrepreneurial system, and sustainable development.
  2. Paving the road towards developing an integrated multidimensional framework for sustainable knowledge economy.

Keywords: Innovation and Entrepreneurial Capacity, Intellectual Capital Development, Sustainable Development, Sustainable Knowledge Economy.

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174 Effect of Different Salt Concentrations and Temperatures on Seed Germination and Seedling Characters in Safflower (Carthamus tinctorius L.) Genotypes

Authors: Rahim Ada, Zamari Temory, Hasan Dalgiç

Abstract:

Germination and seedling responses of seven safflower seed genotypes (Dinçer, Remzibey, Black Sun2 cultivars and A19, F4, I1, J19 lines) to different salinity concentrations (0, 5, 10 and 20g l-1) and temperatures (10 and 20oC) evaluated in Completely Randomized Factorial Designs in Department of Field Crops of Selcuk University, Konya, Turkey. Seeds in the control (distilled water) had at 10 and 20oC the highest germination percentage (93.88 and 94.32%), shoot length (4.60 and 8.72cm) and root length (4.27 and 6.54cm) shoot dry weight (22.37mg and 25.99mg) and root dry weight (2.22 and 2.47mg). As the salt concentration increased, values of all characters were decreased. In this experiment, in 20g l-1 salt concentration found germination percentage (21.28 and 26.66%), shoot (1.32 and 1.35cm) and root length (1.04 and 1.10cm) shoot (8.05mg and 7.49mg) and root dry weight (0.83 and 0.98mg) at 10 and 20oC.

Keywords: NaCl, Safflower, Temperature.

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173 OPTIMAL Placement of FACTS Devices by Genetic Algorithm for the Increased Load Ability of a Power System

Authors: A. B.Bhattacharyya, B. S.K.Goswami

Abstract:

This paper presents Genetic Algorithm (GA) based approach for the allocation of FACTS (Flexible AC Transmission System) devices for the improvement of Power transfer capacity in an interconnected Power System. The GA based approach is applied on IEEE 30 BUS System. The system is reactively loaded starting from base to 200% of base load. FACTS devices are installed in the different locations of the power system and system performance is noticed with and without FACTS devices. First, the locations, where the FACTS devices to be placed is determined by calculating active and reactive power flows in the lines. Genetic Algorithm is then applied to find the amount of magnitudes of the FACTS devices. This approach of GA based placement of FACTS devices is tremendous beneficial both in terms of performance and economy is clearly observed from the result obtained.

Keywords: FACTS Devices, Line Power Flow, OptimalLocation of FACTS Devices, Genetic Algorithm.

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172 Casting Users- Perspectives on Foundries as Suppliers

Authors: T. Saarelainen, O. Piha, P. Makkonen, J. Orkas

Abstract:

Global competition is tightening and companies have to think how to remain competitive. The main aim of this paper is to discuss how Finnish foundries will remain competitive. To fulfil the aim, we conducted interviews in nine companies using castings and analysed buyer–supplier relationships, current competitive advantages of Finnish foundries and customer perspectives on how Finnish foundries remain competitive. We found that the customerfoundry relationship is still closer to traditional subcontracting than partnering and general image of foundries is negative. Current competitive advantages of Finnish foundries include designing cooperation, proximity and flexibility. Casting users state that Finnish foundries should sell their know-how and services instead of their capacity, concentrate on prototype, single and short series castings and supply ready-to-install cast components directly to customers- assembly lines.

Keywords: Buyer-supplier relationship, casting, competitiveadvantage, customer prespective.

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171 Iterative Joint Power Control and Partial Crosstalk Cancellation in Upstream VDSL

Authors: H. Bagheri, H. Emami, M. R. Pakravan

Abstract:

Crosstalk is the major limiting issue in very high bit-rate digital subscriber line (VDSL) systems in terms of bit-rate or service coverage. At the central office side, joint signal processing accompanied by appropriate power allocation enables complex multiuser processors to provide near capacity rates. Unfortunately complexity grows with the square of the number of lines within a binder, so by taking into account that there are only a few dominant crosstalkers who contribute to main part of crosstalk power, the canceller structure can be simplified which resulted in a much lower run-time complexity. In this paper, a multiuser power control scheme, namely iterative waterfilling, is combined with previously proposed partial crosstalk cancellation approaches to demonstrate the best ever achieved performance which is verified by simulation results.

Keywords: iterative waterfilling, partial crosstalk cancellation, run-time complexity, VDSL.

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170 Numerical Evaluation of Nusselt Number on the Hot Wall in Square Enclosure Filled with Nanofluid

Authors: A. Ghafouri, A. Falavand Jozaei, M. Salari

Abstract:

In this paper, effects of using Alumina-water nanofluid on the rate of heat transfer have been investigated numerically. Physical model is a square enclosure with insulated top and bottom horizontal walls, while the vertical walls are kept at different constant temperatures. Two appropriate models are used to evaluate the viscosity and thermal conductivity of nanofluid. The governing stream-vorticity equations are solved using a second order central finite difference scheme, coupled to the conservation of mass and energy. The study has been carried out for the Richardson number 0.1 to 10 and the solid volume fraction 0 to 0.04. Results are presented by isotherms lines, average Nusselt number and normalized Nusselt number in different range of φ and Ri for forced, combined and natural convection dominated regime. It is found that higher heat transfer rate is predicted when the effects of nanoparticle is taken into account.

Keywords: Nanofluid, Heat Transfer Enhancement, Square Enclosure, Nusselt number.

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169 The Evaluation of Production Line Performance by Using ARENA – A Case Study

Authors: Muhammad Marsudi, Hani Shafeek

Abstract:

The purpose of this paper is to simulate the production process of a metal stamping industry and to evaluate the utilization of the production line by using ARENA simulation software. The process time and the standard time for each process of the production line is obtained from data given by the company management. Other data are collected through direct observation of the line. There are three work stations performing ten different types of processes in order to produce a single product type. Arena simulation model is then developed based on the collected data. Verification and validation are done to the Arena model, and finally the result of Arena simulation can be analyzed. It is found that utilization at each workstation will increase if batch size is increased although throughput rate remains/is kept constant. This study is very useful for the company because the company needs to improve the efficiency and utilization of its production lines.

Keywords: Arena software, case study, production line, utilization.

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168 Virtual Container Yard: A Paradigm Shift in Container Inventory Management

Authors: Lalith Edirisinghe, Zhihong Jin, A.W. Wijeratne, Hansa Edirisinghe, Lakshmi Ranwala Rashika Mudunkotuwa

Abstract:

A paradigm shift in container inventory management (CIM) is a long-awaited industry need. Virtual container yard (VCY) is a concept developed in 2013 and its primary objective is to minimize shipping transport cost through implementing container exchange between carriers. Shipping lines always try to maintain lower container idle time and provide higher customer satisfaction. However, it is disappointing to note that carriers turn a blind eye to the escalating cost resulted from the present inefficient CIM mechanism. The cost of empty container management is simply transferred to the importers and exporters as freight adjustments. It also creates an environmental hazard. Therefore, it has now become a problem for the society. Therefore, a paradigm shift may be required as the present CIM system is not working for common interests of human beings as it should be.

Keywords: Virtual container yard, imbalance, management, inventory, container inventory management.

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167 Inhibiting Gene for a Late-Heading Gene Responsible for Photoperiod Sensitivity in Rice (Oryza sativa)

Authors: Amol Dahal, Shunsuke Hori, Haruki Nakazawa, Kazumitsu Onishi, Toshio Kawano, Masayuki Murai

Abstract:

Two indica varieties, IR36 and ‘Suweon 258’ (“S”) are middle-heading in southern Japan. 36U, also middle-heading, is an isogenic line of IR36 carrying Ur1 (Undulate rachis-1) gene. However, late-heading plants segregated in the F2 population from the F1 of S × 36U, and so did in the following generations. The concerning lateness gene is designated as Ex. From the F8 generation, isogenic-line pair of early-heading and late-heading lines, denoted by “E” (ex/ex) and “L” (Ex/Ex), were developed. Genetic analyses of heading time were conducted, using F1s and F2s among L, E, S and 36U. The following inferences were drawn from the experimental results: 1) L, and both of E and 36U harbor Ex and ex, respectively; 2) Besides Ex, S harbors an inhibitor gene to it, i.e. I-Ex which is a novel finding of the present study. 3) Ex is a dominant allele at the E1 locus.

Keywords: Basic vegetative phase, heading time, lateness gene, photoperiod-sensitive phase.

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166 Experimental Study on the Vibration Isolation Performance of Metal-Net Rubber Vibration Absorber

Authors: Su Yi Ming, Hou Ying, Zou Guang Ping

Abstract:

Metal-net rubber is a new dry friction damping material, compared with the traditional metal rubber, which has high mechanization degree, and the mechanical performance of metal-net rubber is more stable. Through the sine sweep experiment and random vibration experiment of metal-net rubber vibration isolator, the influence of several important factors such as the lines slope, relative density and wire diameter on the transfer rate, natural frequency and root-mean-square response acceleration of metal-net rubber vibration isolation system, were studied through the method of control variables. Also, several relevant change curves under different vibration levels were derived, and the effects of vibration level on the natural frequency and root-mean-square response acceleration were analyzed through the curves.

Keywords: Metal-net rubber vibration isolator, relative density, vibration level, wire diameter.

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165 Software Industrialization in Systems Integration

Authors: Matthias Minich, B. Harriehausen-Muehlbauer, C. Wentzel

Abstract:

Today-s economy is in a permanent change, causing merger and acquisitions and co operations between enterprises. As a consequence, process adaptations and realignments result in systems integration and software development projects. Processes and procedures to execute such projects are still reliant on craftsman-ship of highly skilled workers. A generally accepted, industrialized production, characterized by high efficiency and quality, seems inevitable. In spite of this, current concepts of software industrialization are aimed at traditional software engineering and do not consider the characteristics of systems integration. The present work points out these particularities and discusses the applicability of existing industrial concepts in the systems integration domain. Consequently it defines further areas of research necessary to bring the field of systems integration closer to an industrialized production, allowing a higher efficiency, quality and return on investment.

Keywords: Software Industrialization, Systems Integration, Software Product Lines, Component Based Development, Model Driven Development.

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164 Wavelet Entropy Based Algorithm for Fault Detection and Classification in FACTS Compensated Transmission Line

Authors: Amany M. El-Zonkoly, Hussein Desouki

Abstract:

Distance protection of transmission lines including advanced flexible AC transmission system (FACTS) devices has been a very challenging task. FACTS devices of interest in this paper are static synchronous series compensators (SSSC) and unified power flow controller (UPFC). In this paper, a new algorithm is proposed to detect and classify the fault and identify the fault position in a transmission line with respect to a FACTS device placed in the midpoint of the transmission line. Discrete wavelet transformation and wavelet entropy calculations are used to analyze during fault current and voltage signals of the compensated transmission line. The proposed algorithm is very simple and accurate in fault detection and classification. A variety of fault cases and simulation results are introduced to show the effectiveness of such algorithm.

Keywords: Entropy calculation, FACTS, SSSC, UPFC, wavelet transform.

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163 Analysis of Electromagnetic Field Effects Using FEM for Transmission Lines Transposition

Authors: S. Tupsie, A. Isaramongkolrak, P. Pao-la-or

Abstract:

This paper presents the mathematical model of electric field and magnetic field in transmission system, which performs in second-order partial differential equation. This research has conducted analyzing the electromagnetic field radiating to atmosphere around the transmission line, when there is the transmission line transposition in case of long distance distribution. The six types of 500 kV transposed HV transmission line with double circuit will be considered. The computer simulation is applied finite element method that is developed by MATLAB program. The problem is considered to two dimensions, which is time harmonic system with the graphical performance of electric field and magnetic field. The impact from simulation of six types long distance distributing transposition will not effect changing of electric field and magnetic field which surround the transmission line.

Keywords: Transposition, Electromagnetic Field, Finite Element Method (FEM), Transmission Line, Computer Simulation

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162 Enhance Power Quality by HVDC System, Comparison Technique between HVDC and HVAC Transmission Systems

Authors: Smko Zangana, Ergun Ercelebi

Abstract:

The alternating current is the main power in all industries and other aspects especially for the short and mid distances, but as far as long a distance which exceeds 500 KMs, using the alternating current technically will face many difficulties and more costs because it's difficult to control the current and also other restrictions. Therefore, recently those reasons led to building transmission lines HVDC to transmit power for long distances. This document presents technical comparison and assessments for power transmission system among distances either ways and studying the stability of the system regarding the proportion of losses in the actual power sent and received between both sides in different systems and also categorizing filters used in the HVDC system and its impact and effect on reducing Harmonic in the power transmission. MATLAB /Simulink simulation software is used to simulate both HVAC & HVDC power transmission system topologies.

Keywords: HVAC power system, HVDC power system, power system simulation (MATLAB), the alternating current, voltage stability.

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161 Low Nonlinear Effects Index-Guiding Nanostructured Photonic Crystal Fiber

Authors: S. Olyaee, M. Seifouri, A. Nikoosohbat, M. Shams Esfand Abadi

Abstract:

Photonic Crystal Fibers (PCFs) can be used in optical communications as transmission lines. For this reason, the PCFs with low confinement loss, low chromatic dispersion, and low nonlinear effects are highly suitable transmission media. In this paper, we introduce a new design of index-guiding nanostructured photonic crystal fiber (IG-NPCF) with ultra-low chromatic dispersion, low nonlinearity effects, and low confinement loss. Relatively low dispersion is achieved in the wavelength range of 1200 to 1600nm using the proposed design. According to the new structure of nanostructured PCF presented in this study, the chromatic dispersion slope is -30(ps/km.nm) and the confinement loss reaches below 10-7 dB/km. While in the wavelength range mentioned above at the same time an effective area of more than 50.2μm2 is obtained.

Keywords: Optical communication systems, nanostructured, index-guiding, dispersion, confinement loss, photonic crystal fiber.

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160 Influence of the Line Parameters in Transmission Line Fault Location

Authors: Marian Dragomir, Alin Dragomir

Abstract:

In the paper, two fault location algorithms are presented for transmission lines which use the line parameters to estimate the distance to the fault. The first algorithm uses only the measurements from one end of the line and the positive and zero sequence parameters of the line, while the second one uses the measurements from both ends of the line and only the positive sequence parameters of the line. The algorithms were tested using a transmission grid transposed in MATLAB. In a first stage it was established a fault location base line, where the algorithms mentioned above estimate the fault locations using the exact line parameters. After that, the positive and zero sequence resistance and reactance of the line were calculated again for different ground resistivity values and then the fault locations were estimated again in order to compare the results with the base line results. The results show that the algorithm which uses the zero sequence impedance of the line is the most sensitive to the line parameters modifications. The other algorithm is less sensitive to the line parameters modification.

Keywords: Estimation algorithms, fault location, line parameters, simulation tool.

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159 Bioinformatic Analysis of Retroelement-Associated Sequences in Human and Mouse Promoters

Authors: Nadezhda M. Usmanova, Nikolai V. Tomilin

Abstract:

Mammalian genomes contain large number of retroelements (SINEs, LINEs and LTRs) which could affect expression of protein coding genes through associated transcription factor binding sites (TFBS). Activity of the retroelement-associated TFBS in many genes is confirmed experimentally but their global functional impact remains unclear. Human SINEs (Alu repeats) and mouse SINEs (B1 and B2 repeats) are known to be clustered in GCrich gene rich genome segments consistent with the view that they can contribute to regulation of gene expression. We have shown earlier that Alu are involved in formation of cis-regulatory modules (clusters of TFBS) in human promoters, and other authors reported that Alu located near promoter CpG islands have an increased frequency of CpG dinucleotides suggesting that these Alu are undermethylated. Human Alu and mouse B1/B2 elements have an internal bipartite promoter for RNA polymerase III containing conserved sequence motif called B-box which can bind basal transcription complex TFIIIC. It has been recently shown that TFIIIC binding to B-box leads to formation of a boundary which limits spread of repressive chromatin modifications in S. pombe. SINEassociated B-boxes may have similar function but conservation of TFIIIC binding sites in SINEs located near mammalian promoters has not been studied earlier. Here we analysed abundance and distribution of retroelements (SINEs, LINEs and LTRs) in annotated sequences of the Database of mammalian transcription start sites (DBTSS). Fractions of SINEs in human and mouse promoters are slightly lower than in all genome but >40% of human and mouse promoters contain Alu or B1/B2 elements within -1000 to +200 bp interval relative to transcription start site (TSS). Most of these SINEs is associated with distal segments of promoters (-1000 to -200 bp relative to TSS) indicating that their insertion at distances >200 bp upstream of TSS is tolerated during evolution. Distribution of SINEs in promoters correlates negatively with the distribution of CpG sequences. Using analysis of abundance of 12-mer motifs from the B1 and Alu consensus sequences in genome and DBTSS it has been confirmed that some subsegments of Alu and B1 elements are poorly conserved which depends in part on the presence of CpG dinucleotides. One of these CpG-containing subsegments in B1 elements overlaps with SINE-associated B-box and it shows better conservation in DBTSS compared to genomic sequences. It has been also studied conservation in DBTSS and genome of the B-box containing segments of old (AluJ, AluS) and young (AluY) Alu repeats and found that CpG sequence of the B-box of old Alu is better conserved in DBTSS than in genome. This indicates that Bbox- associated CpGs in promoters are better protected from methylation and mutation than B-box-associated CpGs in genomic SINEs. These results are consistent with the view that potential TFIIIC binding motifs in SINEs associated with human and mouse promoters may be functionally important. These motifs may protect promoters from repressive histone modifications which spread from adjacent sequences. This can potentially explain well known clustering of SINEs in GC-rich gene rich genome compartments and existence of unmethylated CpG islands.

Keywords: Retroelement, promoter, CpG island, DNAmethylation.

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158 Hybrid TOA/AOA Schemes for Mobile Location in Cellular Communication Systems

Authors: Chien-Sheng Chen, Szu-Lin Su, Chuan-Der Lu

Abstract:

Wireless location is to determine the mobile station (MS) location in a wireless cellular communications system. When fewer base stations (BSs) may be available for location purposes or the measurements with large errors in non-line-of-sight (NLOS) environments, it is necessary to integrate all available heterogeneous measurements to achieve high location accuracy. This paper illustrates a hybrid proposed schemes that combine time of arrival (TOA) at three BSs and angle of arrival (AOA) information at the serving BS to give a location estimate of the MS. The proposed schemes mitigate the NLOS effect simply by the weighted sum of the intersections between three TOA circles and the AOA line without requiring a priori information about the NLOS error. Simulation results show that the proposed methods can achieve better accuracy when compare with Taylor series algorithm (TSA) and the hybrid lines of position algorithm (HLOP).

Keywords: Time of arrival (TOA), angle of arrival (AOA), non-line-of-sight (NLOS).

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157 Metal-Based Anticancer Agents: In vitro DNA Binding, Cleavage and Cytotoxicity

Authors: Mala Nath, Nagamani Kompelli, Partha Roy, Snehasish Das

Abstract:

Two new metal-based anticancer chemotherapeutic agents, [(Ph2Sn)2(HGuO)2(phen)Cl2] 1 and [(Ph3Sn)(HGuO)(phen)]- Cl.CH3OH.H2O 2, were designed, prepared and characterized by analytical and spectral (IR, ESI-Mass, 1H, 13C and 119Sn NMR) techniques. The proposed geometry of Sn(IV) in 1 and 2 is distorted octahedral and distorted trigonal-bipyramidal, respectively. Both 1 and 2 exhibit potential cytotoxicity in vitro against MCF-7, HepG-2 and DU-145 cell lines. The intrinsic binding constant (Kb) values of 1 (2.33 × 105 M-1) and 2 (2.46 × 105 M-1) evaluated from UV-Visible absorption studies suggest non-classical electrostatic mode of interaction via phosphate backbone of DNA double helix. The Stern- Volmer quenching constant (Ksv) of 1 (9.74 × 105 M-1) and 2 (2.9 × 106 M-1) determined by fluorescence studies suggests the groove binding and intercalation mode for 1 and 2, respectively. Effective cleavage of pBR322 DNA is induced by 1.Their interaction with DNA of cancer cells may account for potency.

Keywords: Anticancer agents, DNA binding studies, NMR spectroscopy, organotin.

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156 A Method of Planar-Template- Based Camera Self-Calibration for Single-View

Authors: Yue Zhao, Chao Li

Abstract:

Camera calibration is an important step in 3D reconstruction. Camera calibration may be classified into two major types: traditional calibration and self-calibration. However, a calibration method in using a checkerboard is intermediate between traditional calibration and self-calibration. A self is proposed based on a square in this paper. Only a square in the planar template, the camera self-calibration can be completed through the single view. The proposed algorithm is that the virtual circle and straight line are established by a square on planar template, and circular points, vanishing points in straight lines and the relation between them are be used, in order to obtain the image of the absolute conic (IAC) and establish the camera intrinsic parameters. To make the calibration template is simpler, as compared with the Zhang Zhengyou-s method. Through real experiments and experiments, the experimental results show that this algorithm is feasible and available, and has a certain precision and robustness.

Keywords: Absolute conic, camera calibration, circle point, vanishing point.

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