Search results for: mutant set minimization.
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 209

Search results for: mutant set minimization.

149 A Holistic Approach for Technical Product Optimization

Authors: H. Lang, M. Bader, A. Buchroithner

Abstract:

Holistic methods covering the development process as a whole – e.g. systems engineering – have established themselves in product design. However, technical product optimization, representing improvements in efficiency and/or minimization of loss, usually applies to single components of a system. A holistic approach is being defined based on a hierarchical point of view of systems engineering. This is subsequently presented using the example of an electromechanical flywheel energy storage system for automotive applications.

Keywords: Design, product development, product optimization, systems engineering, flywheel energy storage.

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148 Two Stage Fuzzy Methodology to Evaluate the Credit Risks of Investment Projects

Authors: O. Badagadze, G. Sirbiladze, I. Khutsishvili

Abstract:

The work proposes a decision support methodology for the credit risk minimization in selection of investment projects. The methodology provides two stages of projects’ evaluation. Preliminary selection of projects with minor credit risks is made using the Expertons Method. The second stage makes ranking of chosen projects using the Possibilistic Discrimination Analysis Method. The latter is a new modification of a well-known Method of Fuzzy Discrimination Analysis.

Keywords: Expert valuations, expertons, investment project risks, positive and negative discriminations, possibility distribution.

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147 Distribution Centers Reliability Cost in Capacitated Facility Location Problem

Authors: Mehdi Seifbarghy, Sajjad Jalali, Seyed Habib A. Rahmati

Abstract:

Recently studies in area of supply chain network (SCN) have focused on the disruption issues in distribution systems. Also this paper extends the previous literature by providing a new biobjective model for cost minimization of designing a three echelon SCN across normal and failure scenarios with considering multi capacity option for manufacturers and distribution centers. Moreover, in order to solve the problem by means of LINGO software, novel model will be reformulated through a branch of LP-Metric method called Min-Max approach.

Keywords: Scenario programming, Distribution, Multi-echelon supply chain design, Reliable facility

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146 Building Gabor Filters from Retinal Responses

Authors: Johannes Partzsch, Christian Mayr, Rene Schuffny

Abstract:

Starting from a biologically inspired framework, Gabor filters were built up from retinal filters via LMSE algorithms. Asubset of retinal filter kernels was chosen to form a particular Gabor filter by using a weighted sum. One-dimensional optimization approaches were shown to be inappropriate for the problem. All model parameters were fixed with biological or image processing constraints. Detailed analysis of the optimization procedure led to the introduction of a minimization constraint. Finally, quantization of weighting factors was investigated. This resulted in an optimized cascaded structure of a Gabor filter bank implementation with lower computational cost.

Keywords: Gabor filter, image processing, optimization

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145 Self-Organizing Maps in Evolutionary Approachmeant for Dimensioning Routes to the Demand

Authors: J.-C. Créput, A. Koukam, A. Hajjam

Abstract:

We present a non standard Euclidean vehicle routing problem adding a level of clustering, and we revisit the use of self-organizing maps as a tool which naturally handles such problems. We present how they can be used as a main operator into an evolutionary algorithm to address two conflicting objectives of route length and distance from customers to bus stops minimization and to deal with capacity constraints. We apply the approach to a real-life case of combined clustering and vehicle routing for the transportation of the 780 employees of an enterprise. Basing upon a geographic information system we discuss the influence of road infrastructures on the solutions generated.

Keywords: Evolutionary algorithm, self-organizing map, clustering and vehicle routing.

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144 A Kernel Classifier using Linearised Bregman Iteration

Authors: K. A. D. N. K Wimalawarne

Abstract:

In this paper we introduce a novel kernel classifier based on a iterative shrinkage algorithm developed for compressive sensing. We have adopted Bregman iteration with soft and hard shrinkage functions and generalized hinge loss for solving l1 norm minimization problem for classification. Our experimental results with face recognition and digit classification using SVM as the benchmark have shown that our method has a close error rate compared to SVM but do not perform better than SVM. We have found that the soft shrinkage method give more accuracy and in some situations more sparseness than hard shrinkage methods.

Keywords: Compressive sensing, Bregman iteration, Generalisedhinge loss, sparse, kernels, shrinkage functions

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143 Stability of Electrical Drives Supplied by a Three Level Inverter

Authors: M. S. Kelaiaia, H. Labar, S. Kelaiaia, T. Mesbah

Abstract:

The development of the power electronics has allowed increasing the precision and reliability of the electrical devices, thanks to the adjustable inverters, as the Pulse Wide Modulation (PWM) applied to the three level inverters, which is the object of this study. The authors treat the relation between the law order adopted for a given system and the oscillations of the electrical and mechanical parameters of which the tolerance depends on the process with which they are integrated (paper factory, lifting of the heavy loads, etc.).Thus, the best choice of the regulation indexes allows us to achieve stability and safety training without investment (management of existing equipment). The optimal behavior of any electric device can be achieved by the minimization of the stored electrical and mechanical energy.

Keywords: Multi level inverter, PWM, Harmonics, oscillation, control.

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142 Ec-A: A Task Allocation Algorithm for Energy Minimization in Multiprocessor Systems

Authors: Anju S. Pillai, T.B. Isha

Abstract:

With the necessity of increased processing capacity with less energy consumption; power aware multiprocessor system has gained more attention in the recent future. One of the additional challenges that is to be solved in a multi-processor system when compared to uni-processor system is job allocation. This paper presents a novel task dependent job allocation algorithm: Energy centric- Allocation (Ec-A) and Rate Monotonic (RM) scheduling to minimize energy consumption in a multiprocessor system. A simulation analysis is carried out to verify the performance increase with reduction in energy consumption and required number of processors in the system.

Keywords: Energy consumption, Job allocation, Multiprocessor systems, Task dependent.

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141 Input-Output Analysis in Laptop Computer Manufacturing

Authors: H. Z. Ulukan, E. Demircioğlu, M. Erol Genevois

Abstract:

The scope of this paper and the aim of proposed model were to apply monetary Input –Output (I-O) analysis to point out the importance of reusing know-how and other requirements in order to reduce the production costs in a manufacturing process for a laptop computer. I-O approach using the monetary input-output model is employed to demonstrate the impacts of different factors in a manufacturing process. A sensitivity analysis showing the correlation between these different factors is also presented. It is expected that the recommended model would have an advantageous effect in the cost minimization process.

Keywords: Input-Output Analysis, Monetary Input-Output Model, Manufacturing Process, Laptop Computer.

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140 A Clock Skew Minimization Technique Considering Temperature Gradient

Authors: Se-Jin Ko, Deok-Min Kim, Seok-Yoon Kim

Abstract:

The trend of growing density on chips has increases not only the temperature in chips but also the gradient of the temperature depending on locations. In this paper, we propose the balanced skew tree generation technique for minimizing the clock skew that is affected by the temperature gradients on chips. We calculate the interconnect delay using Elmore delay equation, and find out the optimal balanced clock tree by modifying the clock trees generated through the Deferred Merge Embedding(DME) algorithm. The experimental results show that the distance variance of clock insertion points with and without considering the temperature gradient can be lowered below 54% and we confirm that the skew is remarkably decreased after applying the proposed technique.

Keywords: clock, clock-skew, temperature, thermal.

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139 The Use of Acid-Aluminium Tolerant Bradyrhizobium japonicum Formula for

Authors: Nisa Rachmania Mubarik, Tedja Imas, Aris Tri Wahyudi , Triadiati , Suharyanto, Happy Widiastuti

Abstract:

Land with low pH soil spread widely in Indonesia can be used for soybean (Glycine max) cultivation, however the production is low. The use of acid tolerant soybean and acidaluminium tolerant nitrogen-fixing bacteria formula was an alternative way to increase soybean productivity on acid soils. Bradyrhizobium japonicum is one of the nitrogen fixing bacteria which can symbiose with soybean plants through root nodule formation. Most of the nitrogen source required by soybean plants can be provided by this symbiosis. This research was conducted to study the influence of acid-aluminium tolerant B. japonicum strain BJ 11 formula using peat as carrier on growth of Tanggamus and Anjasmoro cultivar soybean planted on acid soil fields (pH 5.0- 5.5). The results showed that the inoculant was able to increase the growth and production of soybean which were grown on fields acid soil at Sukadana (Lampung) and Tanah Laut (South Kalimantan), Indonesia.

Keywords: Bradyrhizobium japonicum, acid-aluminium tolerant mutant, Tanggamus cultivar soybean, acid soils

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138 Meshed Antenna for Ku-band Wireless Communication

Authors: Chokri Baccouch, Chayma Bahhar, Hedi Sakli, Nizar Sakli

Abstract:

In this article, we present the combination of an antenna patch structure with a photovoltaic cell in one device for telecommunication applications in isolated environments. The radiating patch element of a patch antenna was replaced by a solar cell. DC current generation is the original feature of the solar cell, but now it was additionally able to receive and transmit electromagnetic waves. A mathematical model which serves in the minimization of power losses of the cell and therefore the improvement in conversion performance was studied. Simulation results of this antenna show a resonance at a frequency of 16.55 GHz in Ku-band with a gain of 4.24 dBi.

Keywords: Electric power collected, optical and electrical losses, optimization of the grid of collection, patch antenna, photovoltaic cell.

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137 An Approximation Method for Exact Boundary Controllability of Euler-Bernoulli System

Authors: Abdelaziz Khernane, Naceur Khelil, Leila Djerou

Abstract:

The aim of this work is to study the numerical implementation of the Hilbert Uniqueness Method for the exact boundary controllability of Euler-Bernoulli beam equation. This study may be difficult. This will depend on the problem under consideration (geometry, control and dimension) and the numerical method used. Knowledge of the asymptotic behaviour of the control governing the system at time T may be useful for its calculation. This idea will be developed in this study. We have characterized as a first step, the solution by a minimization principle and proposed secondly a method for its resolution to approximate the control steering the considered system to rest at time T.

Keywords: Boundary control, exact controllability, finite difference methods, functional optimization.

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136 Minimizing Examinee Collusion with a Latin- Square Treatment Structure

Authors: M. H. Omar

Abstract:

Cheating on standardized tests has been a major concern as it potentially minimizes measurement precision. One major way to reduce cheating by collusion is to administer multiple forms of a test. Even with this approach, potential collusion is still quite large. A Latin-square treatment structure for distributing multiple forms is proposed to further reduce the colluding potential. An index to measure the extent of colluding potential is also proposed. Finally, with a simple algorithm, the various Latin-squares were explored to find the best structure to keep the colluding potential to a minimum.

Keywords: Colluding pairs, Scale for Colluding Potential, Latin-Square Structure, Minimization of Cheating.

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135 Genetic Algorithm and Padé-Moment Matching for Model Order Reduction

Authors: Shilpi Lavania, Deepak Nagaria

Abstract:

A mixed method for model order reduction is presented in this paper. The denominator polynomial is derived by matching both Markov parameters and time moments, whereas numerator polynomial derivation and error minimization is done using Genetic Algorithm. The efficiency of the proposed method can be investigated in terms of closeness of the response of reduced order model with respect to that of higher order original model and a comparison of the integral square error as well.

Keywords: Model Order Reduction (MOR), control theory, Markov parameters, time moments, genetic algorithm, Single Input Single Output (SISO).

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134 Design Optimization of the Primary Containment Building of a Pressurized Water Reactor

Authors: M. Hossain, A. H. Khan, M. A. R. Sarkar

Abstract:

Primary containment structure is one of the five safety layers of a nuclear facility which is needed to be designed in such a manner that it can withstand the pressure and excessive radioactivity during accidental situations. It is also necessary to ensure minimization of cost with maximum possible safety in order to make the design economically feasible and attractive. This paper attempts to identify the optimum design conditions for primary containment structure considering both mechanical and radiation safety keeping the economic aspects in mind. This work takes advantage of commercial simulation software to identify the suitable conditions without the requirement of costly experiments. Generated data may be helpful for further studies.

Keywords: PWR, concrete containment, finite element approach, neutron attenuation, Von Mises Stress.

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133 Reliability Improvement with Optimal Placement of Distributed Generation in Distribution System

Authors: N. Rugthaicharoencheep, T. Langtharthong

Abstract:

This paper presents the optimal placement and sizing of distributed generation (DG) in a distribution system. The problem is to reliability improvement of distribution system with distributed generations. The technique employed to solve the minimization problem is based on a developed Tabu search algorithm and reliability worth analysis. The developed methodology is tested with a distribution system of Roy Billinton Test System (RBTS) bus 2. It can be seen from the case study that distributed generation can reduce the customer interruption cost and therefore improve the reliability of the system. It is expected that our proposed method will be utilized effectively for distribution system operator.

Keywords: Distributed generation Optimization technique Reliability improvement, Distribution system.

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132 Production of IAA by Bradyrhizobium sp.

Authors: Nisa Rachmania Mubarik, Irni Mahagiani, Aris Tri Wahyudi

Abstract:

The objective of this research was to determine the potency of indigenous acid-aluminium tolerant Bradyrhizobium japonicum as producer of indole acetic acid (IAA) and applied it as nitrogen fixation on local soybeans viz Anjasmoro, Tanggamus (yellow soybean seeds), and Detam (black soybean seed). Three isolates of acid-aluminium tolerant Bradyrhizobium japonicum (BJ) were used in this research, i.e. BJ 11 (wt), BJ 11 (19) - BJ 11(wt) mutant, and USDA 110 as a reference isolate. All of isolates tested to produce the IAA by using Salkowsky method. Effect of IAA production by each of B. japonicum was tested on growth pouch and greenhouse using three varieties of soybean. All isolates could grow well and produce IAA on yeast mannitol broth (YMB) medium in the presence of 0.5 mM L-tryptophan. BJ 11 (19) produced the highest of IAA at 4 days incubation compared to BJ 11 (wt) and USDA 110. All tested isolates of Bradyrhizobium japonicum have showed effect on stimulating the formation of root nodules in soybean varieties grown on Leonard bottle. The concentration of IAA on root nodules of soybean symbiotic with B. japonicum was significantly different with control, except on the treatment using Tanggamus soybean.

Keywords: Acid-aluminium tolerant isolate, Bradyrhizobium japonicum, indole acetic acid, soybean.

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131 Enumerative Search for Crane Schedule in Anodizing Operations

Authors: Kanate Pantusavase, Jaramporn Hassamontr

Abstract:

This research aims to develop an algorithm to generate a schedule of multiple cranes that will maximize load throughputs in anodizing operation. The algorithm proposed utilizes an enumerative strategy to search for constant time between successive loads and crane covering range over baths. The computer program developed is able to generate a near-optimal crane schedule within reasonable times, i.e. within 10 minutes. Its results are compared with existing solutions from an aluminum extrusion industry. The program can be used to generate crane schedules for mixed products, thus allowing mixed-model line balancing to improve overall cycle times.

Keywords: Crane scheduling, anodizing operations, cycle time minimization.

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130 On-line Image Mosaicing of Live Stem Cells

Authors: Alessandro Bevilacqua, Alessandro Gherardi, Filippo Piccinini

Abstract:

Image mosaicing is a technique that permits to enlarge the field of view of a camera. For instance, it is employed to achieve panoramas with common cameras or even in scientific applications, to achieve the image of a whole culture in microscopical imaging. Usually, a mosaic of cell cultures is achieved through using automated microscopes. However, this is often performed in batch, through CPU intensive minimization algorithms. In addition, live stem cells are studied in phase contrast, showing a low contrast that cannot be improved further. We present a method to study the flat field from live stem cells images even in case of 100% confluence, this permitting to build accurate mosaics on-line using high performance algorithms.

Keywords: Microscopy, image mosaicing, stem cells.

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129 Fast Segmentation for the Piecewise Smooth Mumford-Shah Functional

Authors: Yingjie Zhang

Abstract:

This paper is concerned with an improved algorithm based on the piecewise-smooth Mumford and Shah (MS) functional for an efficient and reliable segmentation. In order to speed up convergence, an additional force, at each time step, is introduced further to drive the evolution of the curves instead of only driven by the extensions of the complementary functions u + and u - . In our scheme, furthermore, the piecewise-constant MS functional is integrated to generate the extra force based on a temporary image that is dynamically created by computing the union of u + and u - during segmenting. Therefore, some drawbacks of the original algorithm, such as smaller objects generated by noise and local minimal problem also are eliminated or improved. The resulting algorithm has been implemented in Matlab and Visual Cµ, and demonstrated efficiently by several cases.

Keywords: Active contours, energy minimization, image segmentation, level sets.

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128 Constrained Particle Swarm Optimization of Supply Chains

Authors: András Király, Tamás Varga, János Abonyi

Abstract:

Since supply chains highly impact the financial performance of companies, it is important to optimize and analyze their Key Performance Indicators (KPI). The synergistic combination of Particle Swarm Optimization (PSO) and Monte Carlo simulation is applied to determine the optimal reorder point of warehouses in supply chains. The goal of the optimization is the minimization of the objective function calculated as the linear combination of holding and order costs. The required values of service levels of the warehouses represent non-linear constraints in the PSO. The results illustrate that the developed stochastic simulator and optimization tool is flexible enough to handle complex situations.

Keywords: stochastic processes, empirical distributions, Monte Carlo simulation, PSO, supply chain management

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127 Optimal Control of Piezo-Thermo-Elastic Beams

Authors: Marwan Abukhaled, Ibrahim Sadek

Abstract:

This paper presents the vibrations suppression of a thermoelastic beam subject to sudden heat input by a distributed piezoelectric actuators. An optimization problem is formulated as the minimization of a quadratic functional in terms of displacement and velocity at a given time and with the least control effort. The solution method is based on a combination of modal expansion and variational approaches. The modal expansion approach is used to convert the optimal control of distributed parameter system into the optimal control of lumped parameter system. By utilizing the variational approach, an explicit optimal control law is derived and the determination of the corresponding displacement and velocity is reduced to solving a set of ordinary differential equations.

Keywords: Optimal control, Thermoelastic beam, variational approach, modal expansion approach

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126 Decision Support System for Hospital Selection in Emergency Medical Services: A Discrete Event Simulation Approach

Authors: D. Tedesco, G. Feletti, P. Trucco

Abstract:

The present study aims to develop a Decision Support System (DSS) to support operational decisions in Emergency Medical Service (EMS) systems regarding the assignment of medical emergency requests to Emergency Departments (ED). This problem is called “hospital selection” and concerns the definition of policies for the selection of the ED to which patients who require further treatment are transported by ambulance. The employed research methodology consists of a first phase of review of the technical-scientific literature concerning DSSs to support the EMS management and, in particular, the hospital selection decision. From the literature analysis, it emerged that current studies mainly focused on the EMS phases related to the ambulance service and consider a process that ends when the ambulance is available after completing a mission. Therefore, all the ED-related issues are excluded and considered as part of a separate process. Indeed, the most studied hospital selection policy turned out to be proximity, thus allowing to minimize the travelling time and to free-up the ambulance in the shortest possible time. The purpose of the present study consists in developing an optimization model for assigning medical emergency requests to the EDs also considering the expected time performance in the subsequent phases of the process, such as the case mix, the expected service throughput times, and the operational capacity of different EDs in hospitals. To this end, a Discrete Event Simulation (DES) model was created to compare different hospital selection policies. The model was implemented with the AnyLogic software and finally validated on a realistic case. The hospital selection policy that returned the best results was the minimization of the Time To Provider (TTP), considered as the time from the beginning of the ambulance journey to the ED at the beginning of the clinical evaluation by the doctor. Finally, two approaches were further compared: a static approach, based on a retrospective estimation of the TTP, and a dynamic approach, focused on a predictive estimation of the TTP which is determined with a constantly updated Winters forecasting model. Findings reveal that considering the minimization of TTP is the best hospital selection policy. It allows to significantly reducing service throughput times in the ED with a negligible increase in travel time. Furthermore, an immediate view of the saturation state of the ED is produced and the case mix present in the ED structures (i.e., the different triage codes) is considered, as different severity codes correspond to different service throughput times. Besides, the use of a predictive approach is certainly more reliable in terms on TTP estimation, than a retrospective approach. These considerations can support decision-makers in introducing different hospital selection policies to enhance EMSs performance.

Keywords: Emergency medical services, hospital selection, discrete event simulation, forecast model.

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125 A Multi-Objective Model for Supply Chain Network Design under Stochastic Demand

Authors: F. Alborzi, H. Vafaei, M.H. Gholami, M.M. S. Esfahani

Abstract:

In this article, the design of a Supply Chain Network (SCN) consisting of several suppliers, production plants, distribution centers and retailers, is considered. Demands of retailers are considered stochastic parameters, so we generate amounts of data via simulation to extract a few demand scenarios. Then a mixed integer two-stage programming model is developed to optimize simultaneously two objectives: (1) minimization the fixed and variable cost, (2) maximization the service level. A weighting method is utilized to solve this two objective problem and a numerical example is made to show the performance of the model.

Keywords: Mixed Integer Programming, Multi-objective Optimization, Stochastic Demand, Supply Chain Design, Two Stage Programming

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124 Vector Control Using Series Iron Loss Model of Induction, Motors and Power Loss Minimization

Authors: Kheldoun Aissa, Khodja Djalal Eddine

Abstract:

The iron loss is a source of detuning in vector controlled induction motor drives if the classical rotor vector controller is used for decoupling. In fact, the field orientation will not be satisfied and the output torque will not truck the reference torque mostly used by Loss Model Controllers (LMCs). In addition, this component of loss, among others, may be excessive if the vector controlled induction motor is driving light loads. In this paper, the series iron loss model is used to develop a vector controller immune to iron loss effect and then an LMC to minimize the total power loss using the torque generated by the speed controller.

Keywords: Field Oriented Controller, Induction Motor, Loss ModelController, Series Iron Loss.

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123 Effect of DG Installation in Distribution System for Voltage Monitoring Scheme

Authors: S. R. A. Rahim, I. Musirin, M. M. Othman, M. H. Hussain

Abstract:

Loss minimization is a long progressing issue mainly in distribution system. Nevertheless its effect led to temperature rise due to significant voltage drop through the distribution line. Thus, compensation scheme should be proper scheduled in the attempt to alleviate the voltage drop phenomenon. Distributed generation has been profoundly known for voltage profile improvement; provided that over-compensation or under-compensation phenomena are avoided. This paper addresses the issue of voltage improvement through different type DG installation. In ensuring optimal sizing and location of the DGs, pre-developed EMEFA technique was made use for this purpose. Incremental loading condition subjected to the system is the concern such that it is beneficial to the power system operator.

Keywords: Distributed generation, EMEFA, power loss, voltage profile.

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122 An Improved Cuckoo Search Algorithm for Voltage Stability Enhancement in Power Transmission Networks

Authors: Reza Sirjani, Nobosse Tafem Bolan

Abstract:

Many optimization techniques available in the literature have been developed in order to solve the problem of voltage stability enhancement in power systems. However, there are a number of drawbacks in the use of previous techniques aimed at determining the optimal location and size of reactive compensators in a network. In this paper, an Improved Cuckoo Search algorithm is applied as an appropriate optimization algorithm to determine the optimum location and size of a Static Var Compensator (SVC) in a transmission network. The main objectives are voltage stability improvement and total cost minimization. The results of the presented technique are then compared with other available optimization techniques.

Keywords: Cuckoo search algorithm, optimization, power system, var compensators, voltage stability.

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121 Multi-Objective Optimization of an Aerodynamic Feeding System Using Genetic Algorithm

Authors: Jan Busch, Peter Nyhuis

Abstract:

Considering the challenges of short product life cycles and growing variant diversity, cost minimization and manufacturing flexibility increasingly gain importance to maintain a competitive edge in today’s global and dynamic markets. In this context, an aerodynamic part feeding system for high-speed industrial assembly applications has been developed at the Institute of Production Systems and Logistics (IFA), Leibniz Universitaet Hannover. The aerodynamic part feeding system outperforms conventional systems with respect to its process safety, reliability, and operating speed. In this paper, a multi-objective optimisation of the aerodynamic feeding system regarding the orientation rate, the feeding velocity, and the required nozzle pressure is presented.

Keywords: Aerodynamic feeding system, genetic algorithm, multi-objective optimization.

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120 Application of Staining Intensity Correlation Analysis to Visualize Protein Colocalizationat a Cellular Level

Authors: Permphan Dharmasaroja

Abstract:

Mutations of the telomeric copy of the survival motor neuron 1 (SMN1) gene cause spinal muscular atrophy. A deletion of the Eef1a2 gene leads to lower motor neuron degeneration in wasted mice. Indirect evidences have been shown that the eEF1A protein family may interact with SMN, and our previous study showed that abnormalities of neuromuscular junctions in wasted mice were similar to those of Smn mutant mice. To determine potential colocalization between SMN and tissue-specific translation elongation factor 1A2 (eEF1A2), an immunochemical analysis of HeLa cells transfected with the plasmid pcDNA3.1(+)C-hEEF1A2- myc and a new quantitative test of colocalization by intensity correlation analysis (ICA) was used to explore the association of SMN and eEF1A2. Here the results showed that eEF1A2 redistributed from the cytoplasm to the nucleus in response to serum and epidermal growth factor. In the cytoplasm, compelling evidence showed that staining for myc-tagged eEF1A2 varied in synchrony with that for SMN, consistent with the formation of a SMN-eEF1A2 complex in the cytoplasm of HeLa cells. These findings suggest that eEF1A2 may colocalize with SMN in the cytoplasm and may be a component of the SMN complex. However, the limitation of the ICA method is an inability to resolve colocalization in components of small organelles such as the nucleus.

Keywords: Intensity correlation analysis, intensity correlation quotient.

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