Search results for: Low resolution face recognition

Authors: N. Greco, S. Impedovo, R.Modugno, G. Pirlo

Abstract:

This paper presents a new technique for generating sets of synthetic classifiers to evaluate abstract-level combination methods. The sets differ in terms of both recognition rates of the individual classifiers and degree of similarity. For this purpose, each abstract-level classifier is considered as a random variable producing one class label as the output for an input pattern. From the initial set of classifiers, new slightly different sets are generated by applying specific operators, which are defined at the purpose. Finally, the sets of synthetic classifiers have been used to estimate the performance of combination methods for abstract-level classifiers. The experimental results demonstrate the effectiveness of the proposed approach.

Keywords: Abstract-level Classifier, Dempster-Shafer Rule, Multi-expert Systems, Similarity Index, System Evaluation

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Authors: Abdulrahman A. Alghamdi, Paul M. Mummery, Mohammad A. Sheikh

Abstract:

In this paper, 3D image based composite unit cell is constructed from high resolution tomographic images. Through-thickness thermal diffusivity and in-plane Young’s modulus are predicted for the composite unit cell. The accuracy of the image based composite unit cell is tested by comparing its results with the experimental results obtained from laser flash and tensile test. The FE predictions are in close agreement with experimental results. Through-thickness thermal diffusivity and in-plane Young’s modulus of a virgin C/C composite are predicted by replacing the properties of air (porosity) with the properties of carbon matrix. The effect of porosity was found to be more profound on thermal diffusivity than young’s modulus.

Keywords: Porosity, C/C composite, image based FE modelling, CMC.

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Authors: Meng-Lung Lin, Qiubing Wang, Fujun Sun, Tzu-How Chu, Yi-Shiang Shiu

Abstract:

The normalized difference vegetation index (NDVI) and normalized difference moisture index (NDMI) derived from the moderate resolution imaging spectroradiometer (MODIS) have been widely used to identify spatial information of drought condition. The relationship between NDVI and NDMI has been analyzed using Pearson correlation analysis and showed strong positive relationship. The drought indices have detected drought conditions and identified spatial extents of drought. A comparison between normal year and drought year demonstrates that the amplitude analysis considered both vegetation and moisture condition is an effective method to identify drought condition. We proposed the amplitude analysis is useful for quick spatial assessment of drought information at a regional scale.

Keywords: NDVI, NDMI, Drought, remote sensing, spatialassessment.

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Authors: S. M. Amin Hosseini, Oriol Pons, Carmen Mendoza Arroyo, Albert de la Fuente

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In the aftermath of a natural disaster, the major challenge most cities and societies face, regardless of their diverse level of prosperity, is to provide temporary housing (TH) for the displaced population (DP). However, the features of TH, which have been applied in previous recovery programs, greatly varied from case to case. This situation demonstrates that providing temporary accommodation for DP in a short period time and usually in great numbers is complicated in terms of satisfying all the beneficiaries’ needs, regardless of the societies’ welfare levels. Furthermore, when previously used strategies are applied to different areas, the chosen strategies are most likely destined to fail, unless the strategies are context and culturally based. Therefore, as the population of disaster-prone cities are increasing, decision-makers need a platform to help to determine all the factors, which caused the outcomes of the prior programs. To this end, this paper aims to assess the problems, requirements, limitations, potential responses, chosen strategies, and their outcomes, in order to determine the main elements that have influenced the TH process. In this regard, and in order to determine a customizable strategy, this study analyses the TH programs of five different cases as: Marmara earthquake, 1999; Bam earthquake, 2003; Aceh earthquake and tsunami, 2004; Hurricane Katrina, 2005; and, L’Aquila earthquake, 2009. The research results demonstrate that the main vertexes of TH are: (1) local characteristics, including local potential and affected population features, (2) TH properties, which needs to be considered in four phases: planning, provision/construction, operation, and second life, and (3) natural hazards impacts, which embraces intensity and type. Accordingly, this study offers decision-makers the opportunity to discover the main vertexes, their subsets, interactions, and the relation between strategies and outcomes based on the local conditions of each case. Consequently, authorities may acquire the capability to design a customizable method in the face of complicated post-disaster housing in the wake of future natural disasters.

Keywords: Post-disaster temporary accommodation, urban resilience, natural disaster, local characteristic.

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Authors: Kasthurirangan Gopalakrishnan, Naga Shashidhar

Abstract:

The mechanical properties of granular solids are dependent on the flow of stresses from one particle to another through inter-particle contact. Although some experimental methods have been used to study the inter-particle contacts in the past, preliminary work with these techniques indicated that they do not have the necessary resolution to distinguish between those contacts that transmit the load and those that do not, especially for systems with a wide distribution of particle sizes. In this research, computer simulations are used to study the nature and distribution of contacts in a compact with wide particle size distribution, representative of aggregate size distribution used in asphalt pavement construction. The packing fraction, the mean number of contacts and the distribution of contacts were studied for different scenarios. A methodology to distinguish and compute the fraction of load-bearing particles and the fraction of space-filling particles (particles that do not transmit any force) is needed for further investigation.

Keywords: Computer simulation, three-dimensional particlepacking, coordination number, asphalt concrete, aggregates.

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Authors: Rabah Haoui

Abstract:

The aim of this work is to analyze a viscous flow in the axisymmetric nozzle taken into account the mesh size both in the free stream and into the boundary layer. The resolution of the Navier- Stokes equations is realized by using the finite volume method to determine the supersonic flow parameters at the exit of convergingdiverging nozzle. The numerical technique uses the Flux Vector Splitting method of Van Leer. Here, adequate time stepping parameter, along with CFL coefficient and mesh size level is selected to ensure numerical convergence. The effect of the boundary layer thickness is significant at the exit of the nozzle. The best solution is obtained with using a very fine grid, especially near the wall, where we have a strong variation of velocity, temperature and shear stress. This study enabled us to confirm that the determination of boundary layer thickness can be obtained only if the size of the mesh is lower than a certain value limits given by our calculations.

Keywords: Supersonic flow, viscous flow, finite volume, nozzle

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Authors: Priyadarshinee Adhikari, Neeta Gargote, Jyothi Digge, B.G. Hogade

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A number of automated shot-change detection methods for indexing a video sequence to facilitate browsing and retrieval have been proposed in recent years. This paper emphasizes on the simulation of video shot boundary detection using one of the methods of the color histogram wherein scaling of the histogram metrics is an added feature. The difference between the histograms of two consecutive frames is evaluated resulting in the metrics. Further scaling of the metrics is performed to avoid ambiguity and to enable the choice of apt threshold for any type of videos which involves minor error due to flashlight, camera motion, etc. Two sample videos are used here with resolution of 352 X 240 pixels using color histogram approach in the uncompressed media. An attempt is made for the retrieval of color video. The simulation is performed for the abrupt change in video which yields 90% recall and precision value.

Keywords: Abrupt change, color histogram, ground-truthing, precision, recall, scaling, threshold.

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Authors: Peter Ndichu Muriuki

Abstract:

The 9/11 suicide attacks in New York, Washington, D.C., and Pennsylvania, triggered a number of security responses both in the United States of America and other Countries in the World. Kenya, which is an ally and a close partner to North America and Europe, was not left behind. While many states had been parties to numerous terrorism conventions, their response in implementing them had been slow and needed this catalyst. This special case offered a window of opportunity for many “security conscious" regimes in cementing their legal-criminological and political security apparatus. At the international level, the 9/11 case led to the hasty adoption of Security Council resolution 1373 in 2001, which called upon states to adopt wide-ranging and comprehensive steps and strategies to combat international terrorism and to become parties to the relevant international conventions and protocols relating to terrorism. Since then, Kenya has responded with speed in devising social-legal-criminological-political actions.

Keywords: Justice, Policing, Security, Terrorism

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Authors: Kong Fah Tee, Lutfor Rahman Khan, Hongshuang Li

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An advanced Monte Carlo simulation method, called Subset Simulation (SS) for the time-dependent reliability prediction for underground pipelines has been presented in this paper. The SS can provide better resolution for low failure probability level with efficient investigating of rare failure events which are commonly encountered in pipeline engineering applications. In SS method, random samples leading to progressive failure are generated efficiently and used for computing probabilistic performance by statistical variables. SS gains its efficiency as small probability event as a product of a sequence of intermediate events with larger conditional probabilities. The efficiency of SS has been demonstrated by numerical studies and attention in this work is devoted to scrutinise the robustness of the SS application in pipe reliability assessment. It is hoped that the development work can promote the use of SS tools for uncertainty propagation in the decision-making process of underground pipelines network reliability prediction.

Keywords: Underground pipelines, Probability of failure, Reliability and Subset Simulation.

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Authors: Alina Trifan, António J. R. Neves

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Although most digital cameras acquire images in a raw format, based on a Color Filter Array that arranges RGB color filters on a square grid of photosensors, most image compression techniques do not use the raw data; instead, they use the rgb result of an interpolation algorithm of the raw data. This approach is inefficient and by performing a lossless compression of the raw data, followed by pixel interpolation, digital cameras could be more power efficient and provide images with increased resolution given that the interpolation step could be shifted to an external processing unit. In this paper, we conduct a survey on the use of lossless compression algorithms with raw Bayer images. Moreover, in order to reduce the effect of the transition between colors that increase the entropy of the raw Bayer image, we split the image into three new images corresponding to each channel (red, green and blue) and we study the same compression algorithms applied to each one individually. This simple pre-processing stage allows an improvement of more than 15% in predictive based methods.

Keywords: Bayer images, CFA, losseless compression, image coding standards.

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Authors: A. M. Azad, M. Alam, C. M. Hussain

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In this paper, we have presented the effect of varying time-delays on performance and stability in the single-channel multirate sampled-data system in hard real-time (RT-Linux) environment. The sampling task require response time that might exceed the capacity of RT-Linux. So a straight implementation with RT-Linux is not feasible, because of the latency of the systems and hence, sampling period should be less to handle this task. The best sampling rate is chosen for the sampled-data system, which is the slowest rate meets all performance requirements. RT-Linux is consistent with its specifications and the resolution of the real-time is considered 0.01 seconds to achieve an efficient result. The test results of our laboratory experiment shows that the multi-rate control technique in hard real-time operating system (RTOS) can improve the stability problem caused by the random access delays and asynchronization.

Keywords: Multi-rate, PID, RT-Linux, Sampled-data, Servo.

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Authors: Ming-Ta Yang, Jin-Lung Guan, Jhy-Cherng Gu

Abstract:

The purpose of this paper is to solve the problem of protecting aerial lines from high impedance faults (HIFs) in distribution systems. This investigation successfully applies 3I0 zero sequence current to solve HIF problems. The feature extraction system based on discrete wavelet transform (DWT) and the feature identification technique found on statistical confidence are then applied to discriminate effectively between the HIFs and the switch operations. Based on continuous wavelet transform (CWT) pattern recognition of HIFs is proposed, also. Staged fault testing results demonstrate that the proposed wavelet based algorithm is feasible performance well.

Keywords: Continuous wavelet transform, discrete wavelet transform, high impedance faults, statistical confidence.

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Authors: Semeh Ben Salem, Sami Naouali, Moetez Sallami

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The increasing amount of collected data has limited the performance of the current analyzing algorithms. Thus, developing new cost-effective algorithms in terms of complexity, scalability, and accuracy raised significant interests. In this paper, a modified effective k-means based algorithm is developed and experimented. The new algorithm aims to reduce the computational load without significantly affecting the quality of the clusterings. The algorithm uses the City Block distance and a new stop criterion to guarantee the convergence. Conducted experiments on a real data set show its high performance when compared with the original k-means version.

Keywords: Pattern recognition, partitional clustering, K-means clustering, Manhattan distance, terrorism data analysis.

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Authors: Chalise Kiran

Abstract:

The learning difficulties of children with disabilities are always a matter of concern when we talk about educational needs and quality education of children with disabilities. This paper is the outcome of the review of the literature focused on the educational needs and learning difficulties of children with disabilities. For the paper, different studies written on children with disabilities and their education were collected through search engines. The literature put together were analyzed from the angle of learning difficulties faced by children with disabilities and the same were used as a precursor to arrive at the findings on the learning of the children. The analysis showed that children with disabilities face learning difficulties. The reasons for these difficulties could be attributed to factors in terms of authority, structure, school environment and behaviors of teachers and parents and the society as a whole.

Keywords: Children with disabilities, learning difficulties, education of children with disabilities, disabled children.

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Authors: Amol Bapuso Rajmane, Pradeep M. Patil, Prakash J. Kulkarni

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The Information Retrieval community is facing the problem of effective representation of Web search results. When we organize web search results into clusters it becomes easy to the users to quickly browse through search results. The traditional search engines organize search results into clusters for ambiguous queries, representing each cluster for each meaning of the query. The clusters are obtained according to the topical similarity of the retrieved search results, but it is possible for results to be totally dissimilar and still correspond to the same meaning of the query. People search is also one of the most common tasks on the Web nowadays, but when a particular person’s name is queried the search engines return web pages which are related to different persons who have the same queried name. By placing the burden on the user of disambiguating and collecting pages relevant to a particular person, in this paper, we have developed an approach that clusters web pages based on the association of the web pages to the different people and clusters that are based on generic entity search.

Keywords: Entity resolution, information retrieval, graph based disambiguation, web people search, clustering.

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Authors: I. López , J. Garay, R. Carreño, Z. Varga

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Monitoring of ecological systems is one of the major issues in ecosystem research. The concepts and methodology of mathematical systems theory provide useful tools to face this problem. In many cases, state monitoring of a complex ecological system consists in observation (measurement) of certain state variables, and the whole state process has to be determined from the observed data. The solution proposed in the paper is the design of an observer system, which makes it possible to approximately recover the state process from its partial observation. The method is illustrated with a trophic chain of resource – producer – primary consumer type and a numerical example is also presented.

Keywords: Monitoring, observer system, trophic chain

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Authors: Luna Shamieh

Abstract:

Various players are part of the game in an asymmetric war, all making efforts to provide human security to their own adherents. Although a fragile state is not able to provide sufficient and comprehensive services, it still provides special services and security to the elite; the insurgents as well provide services and security to their associates. The humanitarian organisations, on the other hand, provide some fundamental elements of human security, but only in the regions, they are able to access when possible (if possible). The counterinsurgents (security forces of the state and intervention forces) operate within a narrow band defined by the vision of the responsibility to protect and the perspective of the resolution of the conflict through combat; hence, the possibility to provide human security is shaken at this end. This article examines how each player provides human security from the perspective of freedom from want in order to secure basic and strategic needs, freedom from fear through providing protection against all kinds of violence, and the freedom to live in dignity. It identifies a vicious cycle caused by the intervention of the different players causing a centrifugal force that may lead to disintegration of the nation under war.

Keywords: Human security, asymmetric war, counter insurgency, fragile state, insurgency.

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Authors: Changhwan Choi, Yongwan Park

Abstract:

In this paper, we propose a method that allows faster and more accurate detection of traffic lights by a vision sensor during driving, DGPS is used to obtain physical location of a traffic light, extract from the image information of the vision sensor only the traffic light area at this location and ascertain if the sign is in operation and determine its form. This method can solve the problem in existing research where low visibility at night or reflection under bright light makes it difficult to recognize the form of traffic light, thus making driving unstable. We compared our success rate of traffic light recognition in day and night road environments. Compared to previous researches, it showed similar performance during the day but 50% improvement at night.

Keywords: Traffic light, Intelligent vehicle, Night, Detection, DGPS (Differential Global Positioning System).

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Authors: Syed Akhter Hossain, M. Lutfar Rahman, Farruk Ahmed

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Bangla Vowel characterization determines the spectral properties of Bangla vowels for efficient synthesis as well as recognition of Bangla vowels. In this paper, Bangla vowels in isolated word have been analyzed based on speech production model within the framework of Analysis-by-Synthesis. This has led to the extraction of spectral parameters for the production model in order to produce different Bangla vowel sounds. The real and synthetic spectra are compared and a weighted square error has been computed along with the error in the formant bandwidths for efficient representation of Bangla vowels. The extracted features produced good representation of targeted Bangla vowel. Such a representation also plays essential role in low bit rate speech coding and vocoders.

Keywords: Speech, vowel, formant, synthesis, spectrum, LPC.

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Authors: Muhammad Afzal, Cao Yihua, Zhao Ming

Abstract:

The present work describes a computational study of aerodynamic characteristics of GLC305 airfoil clean and with 16.7 min ice shape (rime 212) and 22.5 min ice shape (glaze 944).The performance of turbulence models SA, Kε, Kω Std, and Kω SST model are observed against experimental flow fields at different Mach numbers 0.12, 0.21, 0.28 in a range of Reynolds numbers 3x106, 6x106, and 10.5x106 on clean and iced aircraft airfoil GLC305. Numerical predictions include lift, drag and pitching moment coefficients at different Mach numbers and at different angle of attacks were done. Accuracy of solutions with respect to the effects of turbulence models, variation of Mach number, initial conditions, grid resolution and grid spacing near the wall made the study much sensitive. Navier Stokes equation based computational technique is used. Results are very close to the experimental results. It has seen that SA and SST models are more efficient than Kε and Kω standard in under study problem.

Keywords: Aerodynamics, Airfoil GLC305, Iced Airfoil, Turbulence Model

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Authors: Chokri Baccouch, Chayma Bahhar, Hedi Sakli, Nizar Sakli, Taoufik Aguili

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This paper presents a hybrid system solar cell antenna for 5G mobile communications networks. We propose here a solar cell antenna with either a front face collection grid or mesh patch. The solar cell antenna of our contribution combines both optical and radiofrequency signals. Thus, we propose two solar cell antenna structures in the frequency bands of future 5G standard respectively in both 2.6 and 3.5 GHz bands. Simulation using the Advanced Design System (ADS) software allows us to analyze and determine the antenna parameters proposed in this work such as the reflection coefficient (S11), gain, directivity and radiated power.

Keywords: Patch antenna, solar cell, DC, RF, 5G.

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Authors: G. Mokryani, M. R. Haghifam, J. Esmaeilpoor

Abstract:

Happening of Ferroresonance phenomenon is one of the reasons of consuming and ruining transformers, so recognition of Ferroresonance phenomenon has a special importance. A novel method for classification of Ferroresonance presented in this paper. Using this method Ferroresonance can be discriminate from other transients such as capacitor switching, load switching, transformer switching. Wavelet transform is used for decomposition of signals and Competitive Neural Network used for classification. Ferroresonance data and other transients was obtained by simulation using EMTP program. Using Daubechies wavelet transform signals has been decomposed till six levels. The energy of six detailed signals that obtained by wavelet transform are used for training and trailing Competitive Neural Network. Results show that the proposed procedure is efficient in identifying Ferroresonance from other events.

Keywords: Competitive Neural Network, Ferroresonance, EMTP program, Wavelet transform.

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Authors: Md. Ataullah, Naveen Chauhan

Abstract:

The Address Resolution Protocol (ARP) is used by computers to map logical addresses (IP) to physical addresses (MAC). However ARP is an all trusting protocol and is stateless which makes it vulnerable to many ARP cache poisoning attacks such as Man-in-the-Middle (MITM) and Denial of service (DoS) attacks. These flaws result in security breaches thus weakening the appeal of the computer for exchange of sensitive data. In this paper we describe ARP, outline several possible ARP cache poisoning attacks and give the detailed of some attack scenarios in network having both wired and wireless hosts. We have analyzed each of proposed solutions, identify their strengths and limitations. Finally get that no solution offers a feasible solution. Hence, this paper presents an efficient and secure version of ARP that is able to cope up with all these types of attacks and is also a feasible solution. It is a stateful protocol, by storing the information of the Request frame in the ARP cache, to reduce the chances of various types of attacks in ARP. It is more efficient and secure by broadcasting ARP Reply frame in the network and storing related entries in the ARP cache each time when communication take place.

Keywords: ARP cache poisoning, MITM, DoS

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Authors: Ashraf Anwar

Abstract:

In this work, I present a review on Sparse Distributed Memory for Small Cues (SDMSCue), a variant of Sparse Distributed Memory (SDM) that is capable of handling small cues. I then conduct and show some cognitive experiments on SDMSCue to test its cognitive soundness compared to SDM. Small cues refer to input cues that are presented to memory for reading associations; but have many missing parts or fields from them. The original SDM failed to handle such a problem. SDMSCue handles and overcomes this pitfall. The main idea in SDMSCue; is the repeated projection of the semantic space on smaller subspaces; that are selected based on the input cue length and pattern. This process allows for Read/Write operations using an input cue that is missing a large portion. SDMSCue is augmented with the use of genetic algorithms for memory allocation and initialization. I claim that SDM functionality is a subset of SDMSCue functionality.

Keywords: Artificial intelligence, recall, recognition, SDM, SDMSCue.

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Authors: Yanti Rosmunie Bujang, Husnayati Hussin

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This paper attempts to discuss the spam issue from the Malaysian e-mail users- perspective. The purpose is to discover how Malaysian users handle the spam e-mail problem. From the experiences we hope to discover the necessary effort needed to be undertaken to face this problem in the context of Malaysia. A survey was conducted to understand how Malaysian individual perceived spam and what they actually do with the spam e-mail they received in their daily life. The findings indicate that the level of awareness on spam issue in action is still low and need some extra effort by government and relevant agencies to increase their level of awareness.

Keywords: E-mail, Malaysia, spam, users' perspective.

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Authors: Alireza Faed, David Forbes

Abstract:

Complaints today have the ability to retain customer loyalty using state of the art systems and strategies in customer relationship management to analyze and respond to a plethora of customer perception. The Majority of companies are not aware of the beneficiary utilization of customer complaints for the sake of quality improvements. Also, some companies have problems determining how resolution of complaints can be profitable. In this study, we will define the problems and ascertain the importance of customer management system on the companies. Furthermore, we will determine the impact of such a system on efficiency, confidence, profitability and customer complaints. Eventually, we will develop methods and address the issues. In this paper, we used an open-ended questionnaire and distributed that to 30 randomly chosen respondents which were the passengers in an airport. We also define three hypotheses for our study and we will validate each of them. Then using frequency, Chi- Square and quality control method we optimized the size of customers- negative feedback and improved the process of customer retention.

Keywords: Complaint, Complaint management system, Customer relationship management, Retention.

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Authors: Khamael M. Abualnaja, Lidija Šiller, Benjamin R. Horrocks

Abstract:

Metal-enhanced Luminescence of silicon nanocrystals (SiNCs) was determined using two different particle sizes of silver nanoparticles (AgNPs). SiNCs have been characterized by scanning electron microscopy (SEM), high resolution transmission electron microscopy (HRTEM), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). It is found that the SiNCs are crystalline with an average diameter of 65 nm and FCC lattice. AgNPs were synthesized using photochemical reduction of AgNO3 with sodium dodecyl sulphate (SDS). The enhanced luminescence of SiNCs by AgNPs was evaluated by confocal Raman microspectroscopy. Enhancement up to x9 and x3 times were observed for SiNCs that mixed with AgNPs which have an average particle size of 100 nm and 30 nm, respectively. Silver NPs-enhanced luminescence of SiNCs occurs as a result of the coupling between the excitation laser light and the plasmon bands of AgNPs; thus this intense field at AgNPs surface couples strongly to SiNCs.

Keywords: Luminescence, Silicon Nanocrystals, Silver Nanoparticles, Surface Enhanced Raman Spectroscopy (SERS).

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Authors: S. Pullteap

Abstract:

In this paper, a fiber based Fabry-Perot interferometer is proposed and demonstrated for a non-contact displacement measurement. A piece of micro-prism which attached to the mechanical vibrator is served as the target reflector. Interference signal is generated from the superposition between the sensing beam and the reference beam within the sensing arm of the fiber sensor. This signal is then converted to the displacement value by using a developed program written in visual Cµ programming with a resolution of λ/8. A classical function generator is operated for controlling the vibrator. By fixing an excitation frequency of 100 Hz and varying the excitation amplitude range of 0.1 – 3 Volts, the output displacements measured by the fiber sensor are obtained from 1.55 μm to 30.225 μm. A reference displacement sensor with a sensitivity of ~0.4 μm is also employed for comparing the displacement errors between both sensors. We found that over the entire displacement range, a maximum and average measurement error are obtained of 0.977% and 0.44% respectively.

Keywords: Non-contact displacement measurement, extrinsicfiber based Fabry-Perot interferometer, interference signal, zerocrossingfringe counting technique.

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Authors: Lijiang Wang, Wei Wang, Yuhong Xu

Abstract:

Lanthanide-doped upconversion nanoparticles which can convert near-infrared lights to visible lights have attracted growing interest because of their great potentials in fluorescence imaging. Upconversion fluorescence imaging technique with excitation in the near-infrared (NIR) region has been used for imaging of biological cells and tissues. However, improving the detection sensitivity and decreasing the absorption and scattering in biological tissues are as yet unresolved problems. In this present study, a novel NIR-reflected multispectral imaging system was developed for upconversion fluorescent imaging in small animals. Based on this system, we have obtained the high contrast images without the autofluorescence when biocompatible UCPs were injected near the body surface or deeply into the tissue. Furthermore, we have extracted respective spectra of the upconversion fluorescence and relatively quantify the fluorescence intensity with the multispectral analysis. To our knowledge, this is the first time to analyze and quantify the upconversion fluorescence in the small animal imaging.

Keywords: Multispectral imaging, near-infrared, upconversion fluorescence imaging, upconversion nanoparticles.

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Authors: Yusmeeraz Yusof, Yoshiyuki Yanagimoto, Shigeyasu Uno, Kazuo Nakazato

Abstract:

We demonstrate a nonfaradaic electrochemical impedance spectroscopy measurement of biochemically modified gold plated electrodes using a two-electrode system. The absence of any redox indicator in the impedance measurements provide more precise and accurate characterization of the measured bioanalyte at molecular resolution. An equivalent electrical circuit of the electrodeelectrolyte interface was deduced from the observed impedance data of saline solution at low and high concentrations. The detection of biomolecular interactions was fundamentally correlated to electrical double-layer variation at modified interface. The investigations were done using 20mer deoxyribonucleic acid (DNA) strands without any label. Surface modification was performed by creating mixed monolayer of the thiol-modified single-stranded DNA and a spacer thiol (mercaptohexanol) by a two-step self-assembly method. The results clearly distinguish between the noncomplementary and complementary hybridization of DNA, at low frequency region below several hundreds Hertz.

Keywords: Biosensor, electrical double-layer, impedance spectroscopy, label free DNA.

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