Search results for: gene name recognition
220 On Figuring the City Characteristics and Landscape in Overall Urban Design: A Case Study in Xiangyang Central City, China
Authors: Guyue Zhu, Liangping Hong
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Chinese overall urban design faces a large number of problems such as the neglect of urban characteristics, generalization of content, and difficulty in implementation. Focusing on these issues, this paper proposes the main points of shaping urban characteristics in overall urban design: focuses on core problems in city function and scale, landscape pattern, historical culture, social resources and modern city style and digs the urban characteristic genes. Then, we put forward “core problem location and characteristic gene enhancement” as a kind of overall urban design technical method. Firstly, based on the main problems in urban space as a whole, for the operability goal, the method extracts the key genes and integrates into the multi-dimension system in a targeted manner. Secondly, hierarchical management and guidance system is established which may be in line with administrative management. Finally, by converting the results, action plan is drawn up that can be dynamically implemented. Based on the above idea and method, a practical exploration has been performed in the case of Xiangyang central city.Keywords: City characteristics, overall urban design, planning implementation, Xiangyang central city.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 949219 Weighted Clustering Coefficient for Identifying Modular Formations in Protein-Protein Interaction Networks
Authors: Zelmina Lubovac, Björn Olsson, Jonas Gamalielsson
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This paper describes a novel approach for deriving modules from protein-protein interaction networks, which combines functional information with topological properties of the network. This approach is based on weighted clustering coefficient, which uses weights representing the functional similarities between the proteins. These weights are calculated according to the semantic similarity between the proteins, which is based on their Gene Ontology terms. We recently proposed an algorithm for identification of functional modules, called SWEMODE (Semantic WEights for MODule Elucidation), that identifies dense sub-graphs containing functionally similar proteins. The rational underlying this approach is that each module can be reduced to a set of triangles (protein triplets connected to each other). Here, we propose considering semantic similarity weights of all triangle-forming edges between proteins. We also apply varying semantic similarity thresholds between neighbours of each node that are not neighbours to each other (and hereby do not form a triangle), to derive new potential triangles to include in module-defining procedure. The results show an improvement of pure topological approach, in terms of number of predicted modules that match known complexes.Keywords: Modules, systems biology, protein interactionnetworks, yeast.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2108218 Real-Time Testing of Steel Strip Welds based on Bayesian Decision Theory
Authors: Julio Molleda, Daniel F. García, Juan C. Granda, Francisco J. Suárez
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One of the main trouble in a steel strip manufacturing line is the breakage of whatever weld carried out between steel coils, that are used to produce the continuous strip to be processed. A weld breakage results in a several hours stop of the manufacturing line. In this process the damages caused by the breakage must be repaired. After the reparation and in order to go on with the production it will be necessary a restarting process of the line. For minimizing this problem, a human operator must inspect visually and manually each weld in order to avoid its breakage during the manufacturing process. The work presented in this paper is based on the Bayesian decision theory and it presents an approach to detect, on real-time, steel strip defective welds. This approach is based on quantifying the tradeoffs between various classification decisions using probability and the costs that accompany such decisions.Keywords: Classification, Pattern Recognition, ProbabilisticReasoning, Statistical Data Analysis.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1411217 Human Motion Capture: New Innovations in the Field of Computer Vision
Authors: Najm Alotaibi
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Human motion capture has become one of the major area of interest in the field of computer vision. Some of the major application areas that have been rapidly evolving include the advanced human interfaces, virtual reality and security/surveillance systems. This study provides a brief overview of the techniques and applications used for the markerless human motion capture, which deals with analyzing the human motion in the form of mathematical formulations. The major contribution of this research is that it classifies the computer vision based techniques of human motion capture based on the taxonomy, and then breaks its down into four systematically different categories of tracking, initialization, pose estimation and recognition. The detailed descriptions and the relationships descriptions are given for the techniques of tracking and pose estimation. The subcategories of each process are further described. Various hypotheses have been used by the researchers in this domain are surveyed and the evolution of these techniques have been explained. It has been concluded in the survey that most researchers have focused on using the mathematical body models for the markerless motion capture.
Keywords: Human Motion Capture, Computer Vision, Vision based, Tracking.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2491216 Data Mining on the Router Logs for Statistical Application Classification
Authors: M. Rahmati, S.M. Mirzababaei
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With the advance of information technology in the new era the applications of Internet to access data resources has steadily increased and huge amount of data have become accessible in various forms. Obviously, the network providers and agencies, look after to prevent electronic attacks that may be harmful or may be related to terrorist applications. Thus, these have facilitated the authorities to under take a variety of methods to protect the special regions from harmful data. One of the most important approaches is to use firewall in the network facilities. The main objectives of firewalls are to stop the transfer of suspicious packets in several ways. However because of its blind packet stopping, high process power requirements and expensive prices some of the providers are reluctant to use the firewall. In this paper we proposed a method to find a discriminate function to distinguish between usual packets and harmful ones by the statistical processing on the network router logs. By discriminating these data, an administrator may take an approach action against the user. This method is very fast and can be used simply in adjacent with the Internet routers.Keywords: Data Mining, Firewall, Optimization, Packetclassification, Statistical Pattern Recognition.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1655215 A Quantitative Study on Japanese Internet User's Awareness to Information Security: Necessity and Importance of Education and Policy
Authors: Toshihiko Takemura, Atsushi Umino
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In this paper, the authors examine whether or not there Institute for Information and Communications Policy shows are differences of Japanese Internet users awareness to information security based on individual attributes by using analysis of variance based on non-parametric method. As a result, generally speaking, it is found that Japanese Internet users' awareness to information security is different by individual attributes. Especially, the authors verify that the users who received the information security education would have rather higher recognition concerning countermeasures than other users including self-educated users. It is suggested that the information security education should be enhanced so that the users may appropriately take the information security countermeasures. In addition, the information security policy such as carrying out "e- net caravan" and "information security seminars" are effective in improving the users' awareness on the information security in Japan.
Keywords: Information security education, variance of analysis, Internet users, information security policy, Web-based survey.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1410214 Using Machine Learning Techniques for Autism Spectrum Disorder Analysis and Detection in Children
Authors: Norah Alshahrani, Abdulaziz Almaleh
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Autism Spectrum Disorder (ASD) is a condition related to issues with brain development that affects how a person recognises and communicates with others which results in difficulties with interaction and communication socially and it is constantly growing. Early recognition of ASD allows children to lead safe and healthy lives and helps doctors with accurate diagnoses and management of conditions. Therefore, it is crucial to develop a method that will achieve good results and with high accuracy for the measurement of ASD in children. In this paper, ASD datasets of toddlers and children have been analyzed. We employed the following machine learning techniques to attempt to explore ASD: Random Forest (RF), Decision Tree (DT), Na¨ıve Bayes (NB) and Support Vector Machine (SVM). Then feature selection was used to provide fewer attributes from ASD datasets while preserving model performance. As a result, we found that the best result has been provided by SVM, achieving 0.98% in the toddler dataset and 0.99% in the children dataset.
Keywords: Autism Spectrum Disorder, ASD, Machine Learning, ML, Feature Selection, Support Vector Machine, SVM.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 599213 Downtrend Algorithm and Hedging Strategy in Futures Market
Authors: S. Masteika, A.V. Rutkauskas, A. Tamosaitis
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The paper investigates downtrend algorithm and trading strategy based on chart pattern recognition and technical analysis in futures market. The proposed chart formation is a pattern with the lowest low in the middle and one higher low on each side. The contribution of this paper lies in the reinforcement of statements about the profitability of momentum trend trading strategies. Practical benefit of the research is a trading algorithm in falling markets and back-test analysis in futures markets. When based on daily data, the algorithm has generated positive results, especially when the market had downtrend period. Downtrend algorithm can be applied as a hedge strategy against possible sudden market crashes. The proposed strategy can be interesting for futures traders, hedge funds or scientific researchers performing technical or algorithmic market analysis based on momentum trend trading.Keywords: trading algorithm, chart pattern, downtrend trading, futures market, hedging
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3360212 A Neuro-Automata Decision Support System for the Control of Late Blight in Tomato Crops
Authors: Gizelle K. Vianna, Gustavo S. Oliveira, Gabriel V. Cunha
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The use of decision support systems in agriculture may help monitoring large fields of crops by automatically detecting the symptoms of foliage diseases. In our work, we designed and implemented a decision support system for small tomatoes producers. This work investigates ways to recognize the late blight disease from the analysis of digital images of tomatoes, using a pair of multilayer perceptron neural networks. The networks outputs are used to generate repainted tomato images in which the injuries on the plant are highlighted, and to calculate the damage level of each plant. Those levels are then used to construct a situation map of a farm where a cellular automata simulates the outbreak evolution over the fields. The simulator can test different pesticides actions, helping in the decision on when to start the spraying and in the analysis of losses and gains of each choice of action.
Keywords: Artificial neural networks, cellular automata, decision support system, pattern recognition.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1057211 Production of H5N1 Hemagglutinin inTrichoplusia ni Larvae by a Novel Bi-cistronic Baculovirus Expression Vector
Authors: Tzyy Rong Jinn, Nguyen Tiep Khac, Tzong Yuan Wu
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Highly pathogenic avian influenza (HPAI) H5N1 viruses have created demand for a cost-effective vaccine to prevent a pandemic of the disease. Here, we report that Trichoplusia ni (T. ni) larvae can act as a cost-effective bioreactor to produce recombinant HA5 (rH5HA) proteins as an potential effective vaccine for chickens. To facilitate the recombinant virus identification, virus titer determination and access the infected larvae, we employed the internal ribosome entry site (IRES) derived from Perina nuda virus (PnV, belongs to insect picorna like Iflavirus genus) to construct a bi-cistronic baculovirus expression vector that can express the rH5HA protein and enhanced green fluorescent protein (EGFP) simultaneously. Western blot analysis revealed that the 70 kDa rH5HA protein and partially cleaved products (40 kDa H5HA1) were generated in T. ni larvae infected with recombinant baculovirus carrying the H5HA gene. These data suggest that the baculovirus-larvae recombinant protein expression system could be a cost-effective platform for H5N1 vaccine production.
Keywords: Avian Influenza, baculovirus, hemagglutinin, Trichoplusia ni larvae
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1817210 Context Generation with Image Based Sensors: An Interdisciplinary Enquiry on Technical and Social Issues and their Implications for System Design
Authors: Julia Moehrmann, Gunter Heidemann, Oliver Siemoneit, Christoph Hubig, Uwe-Philipp Kaeppeler, Paul Levi
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Image data holds a large amount of different context information. However, as of today, these resources remain largely untouched. It is thus the aim of this paper to present a basic technical framework which allows for a quick and easy exploitation of context information from image data especially by non-expert users. Furthermore, the proposed framework is discussed in detail concerning important social and ethical issues which demand special requirements in system design. Finally, a first sensor prototype is presented which meets the identified requirements. Additionally, necessary implications for the software and hardware design of the system are discussed, rendering a sensor system which could be regarded as a good, acceptable and justifiable technical and thereby enabling the extraction of context information from image data.Keywords: Context-aware computing, ethical and social issues, image recognition, requirements in system design.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1667209 Methods for Distinction of Cattle Using Supervised Learning
Authors: Radoslav Židek, Veronika Šidlová, Radovan Kasarda, Birgit Fuerst-Waltl
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Machine learning represents a set of topics dealing with the creation and evaluation of algorithms that facilitate pattern recognition, classification, and prediction, based on models derived from existing data. The data can present identification patterns which are used to classify into groups. The result of the analysis is the pattern which can be used for identification of data set without the need to obtain input data used for creation of this pattern. An important requirement in this process is careful data preparation validation of model used and its suitable interpretation. For breeders, it is important to know the origin of animals from the point of the genetic diversity. In case of missing pedigree information, other methods can be used for traceability of animal´s origin. Genetic diversity written in genetic data is holding relatively useful information to identify animals originated from individual countries. We can conclude that the application of data mining for molecular genetic data using supervised learning is an appropriate tool for hypothesis testing and identifying an individual.
Keywords: Genetic data, Pinzgau cattle, supervised learning.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2318208 Estimation of Skew Angle in Binary Document Images Using Hough Transform
Authors: Nandini N., Srikanta Murthy K., G. Hemantha Kumar
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This paper includes two novel techniques for skew estimation of binary document images. These algorithms are based on connected component analysis and Hough transform. Both these methods focus on reducing the amount of input data provided to Hough transform. In the first method, referred as word centroid approach, the centroids of selected words are used for skew detection. In the second method, referred as dilate & thin approach, the selected characters are blocked and dilated to get word blocks and later thinning is applied. The final image fed to Hough transform has the thinned coordinates of word blocks in the image. The methods have been successful in reducing the computational complexity of Hough transform based skew estimation algorithms. Promising experimental results are also provided to prove the effectiveness of the proposed methods.Keywords: Dilation, Document processing, Hough transform, Optical Character Recognition, Skew estimation, and Thinning.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3267207 Providing a Secure Hybrid Method for Graphical Password Authentication to Prevent Shoulder Surfing, Smudge and Brute Force Attack
Authors: Faraji Sepideh
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Nowadays, purchase rate of the smart device is increasing and user authentication is one of the important issues in information security. Alphanumeric strong passwords are difficult to memorize and also owners write them down on papers or save them in a computer file. In addition, text password has its own flaws and is vulnerable to attacks. Graphical password can be used as an alternative to alphanumeric password that users choose images as a password. This type of password is easier to use and memorize and also more secure from pervious password types. In this paper we have designed a more secure graphical password system to prevent shoulder surfing, smudge and brute force attack. This scheme is a combination of two types of graphical passwords recognition based and Cued recall based. Evaluation the usability and security of our proposed scheme have been explained in conclusion part.
Keywords: Brute force attack, graphical password, shoulder surfing attack, smudge attack.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 913206 A Study of Distinctive Models for Pre-hospital EMS in Thailand: Knowledge Capture
Authors: R. Sinthavalai, N. Memongkol, N. Patthanaprechawong, J. Viriyanantavong, C. Choosuk
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In Thailand, the practice of pre-hospital Emergency Medical Service (EMS) in each area reveals the different growth rates and effectiveness of the practices. Those can be found as the diverse quality and quantity. To shorten the learning curve prior to speed-up the practices in other areas, story telling and lessons learnt from the effective practices are valued as meaningful knowledge. To this paper, it was to ascertain the factors, lessons learnt and best practices that have impact as contributing to the success of prehospital EMS system. Those were formulized as model prior to speedup the practice in other areas. To develop the model, Malcolm Baldrige National Quality Award (MBNQA), which is widely recognized as a framework for organizational quality assessment and improvement, was chosen as the discussion framework. Remarkably, this study was based on the consideration of knowledge capture; however it was not to complete the loop of knowledge activities. Nevertheless, it was to highlight the recognition of knowledge capture, which is the initiation of knowledge management.Keywords: Emergency Medical Service, Modeling, MBNQA, Thailand.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1559205 Fingerprint Verification System Using Minutiae Extraction Technique
Authors: Manvjeet Kaur, Mukhwinder Singh, Akshay Girdhar, Parvinder S. Sandhu
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Most fingerprint recognition techniques are based on minutiae matching and have been well studied. However, this technology still suffers from problems associated with the handling of poor quality impressions. One problem besetting fingerprint matching is distortion. Distortion changes both geometric position and orientation, and leads to difficulties in establishing a match among multiple impressions acquired from the same finger tip. Marking all the minutiae accurately as well as rejecting false minutiae is another issue still under research. Our work has combined many methods to build a minutia extractor and a minutia matcher. The combination of multiple methods comes from a wide investigation into research papers. Also some novel changes like segmentation using Morphological operations, improved thinning, false minutiae removal methods, minutia marking with special considering the triple branch counting, minutia unification by decomposing a branch into three terminations, and matching in the unified x-y coordinate system after a two-step transformation are used in the work.
Keywords: Biometrics, Minutiae, Crossing number, False Accept Rate (FAR), False Reject Rate (FRR).
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 6576204 Influencing Factors of Residents’ Intention to Participate in the Governance of Old Community Renewal: A Case Study of Nanjing
Authors: Tiantian Gu, Dezhi Li, Mian Zhang, Ying Jiang
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Considering the characteristics of residents’ participation in the governance of old community renewal (OCR), a theoretical model of the determinant of residents’ intention to participate in the governance of OCR has been built based on the theory of planned behavior. Seven old communities in Nanjing have been chosen as cases to conduct empirical analysis. The result indicates that participation attitude, subjective norm and perceived behavioral control have significant positive effects on residents’ intention to participate in the governance of the OCR. Recognition of the community, cognition of the OCR and perceived behavioral control have indirect positive effects on residents’ intention to participate in the OCR. In addition, the education level and the length of residence have positive effects on their participation intention, while the gender, age, and monthly income have little effect on it. The research result provides suggestions for the improvement of residents’ participation in the OCR.Keywords: Old community renewal, residents’ participation in governance, intention, theory of planned behavior.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 760203 Input Textural Feature Selection By Mutual Information For Multispectral Image Classification
Authors: Mounir Ait kerroum, Ahmed Hammouch, Driss Aboutajdine
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Texture information plays increasingly an important role in remotely sensed imagery classification and many pattern recognition applications. However, the selection of relevant textural features to improve this classification accuracy is not a straightforward task. This work investigates the effectiveness of two Mutual Information Feature Selector (MIFS) algorithms to select salient textural features that contain highly discriminatory information for multispectral imagery classification. The input candidate features are extracted from a SPOT High Resolution Visible(HRV) image using Wavelet Transform (WT) at levels (l = 1,2). The experimental results show that the selected textural features according to MIFS algorithms make the largest contribution to improve the classification accuracy than classical approaches such as Principal Components Analysis (PCA) and Linear Discriminant Analysis (LDA).Keywords: Feature Selection, Texture, Mutual Information, Wavelet Transform, SVM classification, SPOT Imagery.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1554202 The Lubrication Regimes Recognition of a Pressure-Fed Journal Bearing by Time and Frequency Domain Analysis of Acoustic Emission Signals
Authors: S. Hosseini, M. Ahmadi Najafabadi, M. Akhlaghi
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The health of the journal bearings is very important in preventing unforeseen breakdowns in rotary machines, and poor lubrication is one of the most important factors for producing the bearing failures. Hydrodynamic lubrication (HL), mixed lubrication (ML), and boundary lubrication (BL) are three regimes of a journal bearing lubrication. This paper uses acoustic emission (AE) measurement technique to correlate features of the AE signals to the three lubrication regimes. The transitions from HL to ML based on operating factors such as rotating speed, load, inlet oil pressure by time domain and time-frequency domain signal analysis techniques are detected, and then metal-to-metal contacts between sliding surfaces of the journal and bearing are identified. It is found that there is a significant difference between theoretical and experimental operating values that are obtained for defining the lubrication regions.Keywords: Acoustic emission technique, pressure fed journal bearing, time and frequency signal analysis, metal-to-metal contact.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 812201 Development of an Organizational Knowledge Capabilities Assessment (OKCA) Method for Innovative Technology Enterprises
Authors: C.F. Cheung, Ricky Ma, W.Y. Wong, Y.L. Tse
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Knowledge capabilities are increasingly important for the innovative technology enterprises to enhance the business performance in terms of product competitiveness, innovation and sales. Recognition of the company capability by auditing allows them to further pursue advancement, strategic planning and hence gain competitive advantages. This paper attempts to develop an Organizations- Knowledge Capabilities Assessment (OKCA) method to assess the knowledge capabilities of technology companies. The OKCA is a questionnaire-based assessment tool which has been developed to uncover the impact of various knowledge capabilities on different organizational performance. The collected data is then analyzed to find out the crucial elements for different technological companies. Based on the results, innovative technology enterprises are able to recognize the direction for further improvement on business performance and future development plan. External environmental factors affecting organization performance can be found through the further analysis of some selected reference companies.Keywords: Audit and Assessment, Innovation, Intellectual Capital, Knowledge and Technology Management, Knowledge Capability
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1878200 Detection of Arcobacter and Helicobacter pylori Contamination in Organic Vegetables by Cultural and PCR Methods
Authors: Miguel García-Ferrús, Ana González, María A. Ferrús
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The most demanded organic foods worldwide are those that are consumed fresh, such as fruits and vegetables. However, there is a knowledge gap about some aspects of organic food microbiological quality and safety. Organic fruits and vegetables are more exposed to pathogenic microorganisms due to surface contact with natural fertilizers such as animal manure, wastes and vermicompost used during farming. Therefore, the objective of this work was to study the contamination of organic fresh green leafy vegetables by two emergent pathogens, Arcobacter spp. and Helicobacter pylori. For this purpose, a total of 24 vegetable samples, 13 lettuce and 11 spinach were acquired from 10 different ecological supermarkets and greengroceries and analyzed by culture and PCR. Arcobacter spp. was detected in five samples (20%) by PCR, four spinach and one lettuce. One spinach sample was found to be also positive by culture. For H. pylori, the H. pylori VacA gene-specific band was detected in 12 vegetable samples (50%), 10 lettuces and two spinach. Isolation in the selective medium did not yield any positive result, possibly because of low contamination levels together with the presence of the organism in its viable but non-culturable form. Results showed significant levels of H. pylori and Arcobacter contamination in organic vegetables that are generally consumed raw, which seems to confirm that these foods can act as transmission vehicles to humans.
Keywords: Arcobacter spp., Helicobacter pylori, organic vegetables, Polymerase Chain Reaction, PCR.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 404199 Development of Innovative Islamic Web Applications
Authors: Farrukh Shahzad
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The rich Islamic resources related to religious text, Islamic sciences, and history are widely available in print and in electronic format online. However, most of these works are only available in Arabic language. In this research, an attempt is made to utilize these resources to create interactive web applications in Arabic, English and other languages. The system utilizes the Pattern Recognition, Knowledge Management, Data Mining, Information Retrieval and Management, Indexing, storage and data-analysis techniques to parse, store, convert and manage the information from authentic Arabic resources. These interactive web Apps provide smart multi-lingual search, tree based search, on-demand information matching and linking. In this paper, we provide details of application architecture, design, implementation and technologies employed. We also presented the summary of web applications already developed. We have also included some screen shots from the corresponding web sites. These web applications provide an Innovative On-line Learning Systems (eLearning and computer based education).Keywords: Islamic resources, Muslim scholars, hadith, narrators, history, fiqh.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1303198 Computational Analysis of Potential Inhibitors Selected Based On Structural Similarity for the Src SH2 Domain
Authors: W. P. Hu, J. V. Kumar, Jeffrey J. P. Tsai
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The inhibition of SH2 domain regulated protein-protein interactions is an attractive target for developing an effective chemotherapeutic approach in the treatment of disease. Molecular simulation is a useful tool for developing new drugs and for studying molecular recognition. In this study, we searched potential drug compounds for the inhibition of SH2 domain by performing structural similarity search in PubChem Compound Database. A total of 37 compounds were screened from the database, and then we used the LibDock docking program to evaluate the inhibition effect. The best three compounds (AP22408, CID 71463546 and CID 9917321) were chosen for MD simulations after the LibDock docking. Our results show that the compound CID 9917321 can produce a more stable protein-ligand complex compared to other two currently known inhibitors of Src SH2 domain. The compound CID 9917321 may be useful for the inhibition of SH2 domain based on these computational results. Subsequently experiments are needed to verify the effect of compound CID 9917321 on the SH2 domain in the future studies.
Keywords: Nonpeptide inhibitor, Src SH2 domain, LibDock, molecular dynamics simulation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2078197 Displaying of GnRH Peptides on Bacteriophage T7 and Its Immunogenicity in Mice Model
Authors: Hai Xu, Yiwei Wang, Xi Bao, Bihua Deng, Pengcheng Li, Yu Lu
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T7 phage could be used as a perfect vector for peptides expression and haptens presentation. T7-3GnRH recombinant phage was constructed by inserting three copies of Gonadotrophin Releasing Hormone (GnRH) gene into the multiple cloning site of T7 Select 415-1b phage genome. The positive T7-3GnRH phage was selected by using polymerase chain reaction amplification, and the p10B-3GnRH fusion protein was verified by SDS-PAGE and Western-blotting assay. T7-3GnRH vaccine was made and immunized with 1010 pfu in 0.2 ml per dose in mice. Blood samples were collected at an interval in weeks, and anti-GnRH antibody and testosterone concentrations were detected by ELISA and radioimmunoassay, respectively. The results show that T7-3GnRH phage particles confer a high immunogenicity to the GnRH-derived epitope. Moreover, the T7-3GnRH vaccine induced higher level of anti-GnRH antibody than ImproVac®. However, the testosterone concentrations in both immunized groups were at a similar level, and the testis developments were significantly inhibited compared to controls. These findings demonstrated that the anti-GnRH antibody could neutralize the endogenous GnRH to down regulate testosterone level and limit testis development, highlighting the potential value of T7-3GnRH in the immunocastration vaccine research.
Keywords: Gonadotrophin releasing hormone, GnRH, immunocastration, T7 phage, phage vaccine.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1110196 Driver Fatigue State Recognition with Pixel Based Caveat Scheme Using Eye-Tracking
Authors: K. Thulasimani, K. G. Srinivasagan
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Driver fatigue is an important factor in the increasing number of road accidents. Dynamic template matching method was proposed to address the problem of real-time driver fatigue detection system based on eye-tracking. An effective vision based approach was used to analyze the driver’s eye state to detect fatigue. The driver fatigue system consists of Face detection, Eye detection, Eye tracking, and Fatigue detection. Initially frames are captured from a color video in a car dashboard and transformed from RGB into YCbCr color space to detect the driver’s face. Canny edge operator was used to estimating the eye region and the locations of eyes are extracted. The extracted eyes were considered as a template matching for eye tracking. Edge Map Overlapping (EMO) and Edge Pixel Count (EPC) matching function were used for eye tracking which is used to improve the matching accuracy. The pixel of eyeball was tracked from the eye regions which are used to determine the fatigue state of the driver.Keywords: Driver fatigue detection, Driving safety, Eye tracking, Intelligent transportation system, Template matching.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1728195 Information Security in E-Learning through Identification of Humans
Authors: Hassan Haleh, Zohreh Nasiri, Parisa Farahpour
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During recent years, the traditional learning approaches have undergone fundamental changes due to the emergence of new technologies such as multimedia, hypermedia and telecommunication. E-learning is a modern world phenomenon that has come into existence in the information age and in a knowledgebased society. E-learning has developed significantly within a short period of time. Thus it is of a great significant to secure information, allow a confident access and prevent unauthorized accesses. Making use of individuals- physiologic or behavioral (biometric) properties is a confident method to make the information secure. Among the biometrics, fingerprint is more acceptable and most countries use it as an efficient methods of identification. This article provides a new method to compare the fingerprint comparison by pattern recognition and image processing techniques. To verify fingerprint, the shortest distance method is used together with perceptronic multilayer neural network functioning based on minutiae. This method is highly accurate in the extraction of minutiae and it accelerates comparisons due to elimination of false minutiae and is more reliable compared with methods that merely use directional images.Keywords: Fingerprint, minutiae, extraction of properties, multilayer neural network
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1649194 Impact of Gold and Silver Nanoparticles on Terrestrial Flora and Microorganisms
Authors: L. Steponavičiūtė, L. Steponavičienė
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Despite the rapid nanotechnology progress and recognition, its potential impact in ecosystems and health of humans is still not fully known. In this paper, the study of ecotoxicological dangers of nanomaterials is presented. By chemical reduction method, silver (AgNPs) and gold (AuNPs) nanoparticles were synthesized, characterized and used in experiments to examine their impact on microorganisms (Escherichia coli, Staphylococcus aureus and Candida albicans) and terrestrial flora (Phaseolus vulgaris and Lepidium sativum). The results collected during experiments with terrestrial flora show tendentious growth stimulations caused by gold nanoparticles. In contrast to these results, silver nanoparticle solutions inhibited growth of beans and garden cress, compared to control samples. The results obtained from experiments with microorganisms show similarities with ones collected from experiments with terrestrial plants. Samples treated with AuNPs of size 13 nm showed stimulation in the growth of the colonies compared with 3,5 nm size nanoparticles.
Keywords: Ecosystems, ecotoxicology, nanomaterials, nanoparticles.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1559193 Identification of Arousal and Relaxation by using SVM-Based Fusion of PPG Features
Authors: Chi Jung Kim, Mincheol Whang, Eui Chul Lee
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In this paper, we propose a new method to distinguish between arousal and relaxation states by using multiple features acquired from a photoplethysmogram (PPG) and support vector machine (SVM). To induce arousal and relaxation states in subjects, 2 kinds of sound stimuli are used, and their corresponding biosignals are obtained using the PPG sensor. Two features–pulse to pulse interval (PPI) and pulse amplitude (PA)–are extracted from acquired PPG data, and a nonlinear classification between arousal and relaxation is performed using SVM. This methodology has several advantages when compared with previous similar studies. Firstly, we extracted 2 separate features from PPG, i.e., PPI and PA. Secondly, in order to improve the classification accuracy, SVM-based nonlinear classification was performed. Thirdly, to solve classification problems caused by generalized features of whole subjects, we defined each threshold according to individual features. Experimental results showed that the average classification accuracy was 74.67%. Also, the proposed method showed the better identification performance than the single feature based methods. From this result, we confirmed that arousal and relaxation can be classified using SVM and PPG features.Keywords: Support Vector Machine, PPG, Emotion Recognition, Arousal, Relaxation
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2484192 A Comparative Study of SVM Classifiers and Artificial Neural Networks Application for Rolling Element Bearing Fault Diagnosis using Wavelet Transform Preprocessing
Authors: Commander Sunil Tyagi
Abstract:
Effectiveness of Artificial Neural Networks (ANN) and Support Vector Machines (SVM) classifiers for fault diagnosis of rolling element bearings are presented in this paper. The characteristic features of vibration signals of rotating driveline that was run in its normal condition and with faults introduced were used as input to ANN and SVM classifiers. Simple statistical features such as standard deviation, skewness, kurtosis etc. of the time-domain vibration signal segments along with peaks of the signal and peak of power spectral density (PSD) are used as features to input the ANN and SVM classifier. The effect of preprocessing of the vibration signal by Discreet Wavelet Transform (DWT) prior to feature extraction is also studied. It is shown from the experimental results that the performance of SVM classifier in identification of bearing condition is better then ANN and pre-processing of vibration signal by DWT enhances the effectiveness of both ANN and SVM classifierKeywords: ANN, Artificial Intelligence, Fault Diagnosis, Pattern Recognition, Rolling Element Bearing, SVM. Wavelet Transform
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2119191 Mining Genes Relations in Microarray Data Combined with Ontology in Colon Cancer Automated Diagnosis System
Authors: A. Gruzdz, A. Ihnatowicz, J. Siddiqi, B. Akhgar
Abstract:
MATCH project [1] entitle the development of an automatic diagnosis system that aims to support treatment of colon cancer diseases by discovering mutations that occurs to tumour suppressor genes (TSGs) and contributes to the development of cancerous tumours. The constitution of the system is based on a) colon cancer clinical data and b) biological information that will be derived by data mining techniques from genomic and proteomic sources The core mining module will consist of the popular, well tested hybrid feature extraction methods, and new combined algorithms, designed especially for the project. Elements of rough sets, evolutionary computing, cluster analysis, self-organization maps and association rules will be used to discover the annotations between genes, and their influence on tumours [2]-[11]. The methods used to process the data have to address their high complexity, potential inconsistency and problems of dealing with the missing values. They must integrate all the useful information necessary to solve the expert's question. For this purpose, the system has to learn from data, or be able to interactively specify by a domain specialist, the part of the knowledge structure it needs to answer a given query. The program should also take into account the importance/rank of the particular parts of data it analyses, and adjusts the used algorithms accordingly.Keywords: Bioinformatics, gene expression, ontology, selforganizingmaps.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1974