Enhancement of Biogas Production from Bakery Waste by Pseudomonas aeruginosa

Authors: S. Potivichayanon, T. Sungmon, W. Chaikongmao, S. Kamvanin

Abstract:

Production of biogas from bakery waste was enhanced by additional bacterial cell. This study was divided into 2 steps. First step, grease waste from bakery industry-s grease trap was initially degraded by Pseudomonas aeruginosa. The concentration of byproduct, especially glycerol, was determined and found that glycerol concentration increased from 12.83% to 48.10%. Secondary step, 3 biodigesters were set up in 3 different substrates: non-degraded waste as substrate in first biodigester, degraded waste as substrate in secondary biodigester, and degraded waste mixed with swine manure in ratio 1:1 as substrate in third biodigester. The highest concentration of biogas was found in third biodigester that was 44.33% of methane and 63.71% of carbon dioxide. The lower concentration at 24.90% of methane and 18.98% of carbon dioxide was exhibited in secondary biodigester whereas the lowest was found in non-degraded waste biodigester. It was demonstrated that the biogas production was greatly increased with the initial grease waste degradation by Pseudomonas aeruginosa.

Keywords: Biogas production, carbon dioxide, methane, Pseudomonas aeruginosa

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1078919

References:

[1] A. Noyola, J. M. Morgan-Sagasrume, and J. E. Lopez-Hernandez, "Treatment of biogas produced in anaerobic reactors for domestic wastewater: odor control and energy/resource recovery," Rev Environ Sci Biotechnol., vol. 5, pp.93-114, 2006.
[2] A. A. Mendes, E.B. Pereira, and H. F. de Castro, "Effect of the enzymatic hydrolysis pretreatment of lipids-rich wastewater on the anaerobic biodigestion," Biochem Eng J., vol. 32, pp.185-190, 2006.
[3] L. M. Svensson, K. Christensson, and L. Bjornsson, "Biogas production from crop residues on a farm-scale level in Sweden: scale, choice of substrate and utilization rate most important parameters for financial feasibility," Bioprocess Biosyst Eng., vol. 29, pp. 137-142, 2006.
[4] S. Luste, S. Luostarinen, and M. Sillanpaa, "Effect of pre-treatments on hydrolysis and methane production potentials of by-products from meatprocessing industry," J Hazard Mater., vol. 164, pp. 247-255, 2009.
[5] O. A. Bolarinwa and E. O. Ugoji, "Production of biogas from starchy wastes," J Sci Res Dev., vol. 12, pp. 34-45, 2010.
[6] C. Wei, T. Zhang, C. Feng, H. Wu, Z. Deng, C. Wu, and B. Lu, "Treatment of food processing wastewater in a full-scale jet biogas internal loop anaerobic fluidized bed reactor," Biodegradation, vol. 22, pp. 347-357, 2011.
[7] Residua, "Anaerobic digestion," Warmer bulletin, North Yorkshire, United Kingdom, 2007, pp. 1-4.
[8] GE Jenbacher, "Biogas engines,"
[online] Available: http://www.clarkeenergy. com, accessed 15.04.11
[9] G. Lettinga, A. F. M. Van Velson, S. W. Hobma, W. De Zeeuw, and A. Klapwijk, "Use of upflow sludge blanket reactor for biological wastewater treatment, especially for anaerobic treatment," Biotechnol Bioeng., vol. 4, pp. 674-699, 1980.
[10] A. Wilkie and E. Colleran, "Pilot scale digestion of pig slurry supernatant using an upflow anaerobic filter," Environ Lett., vol. 7, pp. 65-76, 1986.
[11] Y. Santosh, T. R. Sreekrishnan, S. Kohli, and V. Rana, "Enhancement of biogas production from solid substrates using different techniques-a review," Bioresour Technol., vol. 95, pp. 1-10, 2004.
[12] G. N. Demirer, M. Duran, T. H. Erguder, E.Guven, O. Ugurlu, and U. Tezel, "Anaerobic treatability and biogas production potential studies of different agro-industrial wastewaters in Turkey," Biodegradation, vol. 11, pp. 401-405, 2000.
[13] N. Azbar, T. Keskin, and A. Yuruyen, "Enhancement of biogas production from olive mill effluent (OME) by co-digestion," Biomass Bioenerg., vol. 32, pp. 1195-1201, 2008.
[14] R. Li, S. Chen, and X. Li, "Biogas production from anaerobic codigestion of food waste with dairy manure in a two-phase digestion system," Appl Biochem Biotechnol., vol. 160, pp. 643-654, 2010.
[15] S. Satyanarayan and R. Shivayogi, "Biogas production enhancement by soya sludge amendment in cattle dung digesters," Biomass Bioenerg., vol. 34, pp. 1278-1282, 2010.
[16] Q. Wei, W. Wei, Z. Cuiping, and Z. Zhongzhi, "Biogas recovery from microwave heated sludge by anaerobic digestion," Sci China Tech Sci., vol. 53, pp. 144-149, 2010.
[17] S. Tirumale and K. Nand, "Influence of anaerobic cellulolytic bacterial consortia in the anaerobic digesters on biogas production," Biogas Forum III, vol. 58, pp. 12-15, 1994.
[18] Y. Attar, S. T. Mhetre, and M. D. Shawale, "Biogas production enhancement by cellulytic strains of Actinomycetes," Biogas Forum I, vol. 72, pp. 11-15, 1998.
[19] L. Masse, K. J. Kennedy, and S. Chou, "Testing of alkaline and enzymatic pretreatment for fat particles in slaughterhouses wastewater," Bioresour Technol., vol. 77, pp. 145-155, 2001.
[20] A. H. Mouneimne, H. Carrere, N. Bernet, and J. P. Delgenes, "Effect of saponification on the anaerobic digester of solid fatty residues," Bioresour Technol., vol. 90, pp. 89-94, 2003.
[21] E. El-Bestawy, M. H. El-Masry, and N. E. El-Adl, "The potentiality of free gram-negative bacteria for removing oil and grease from contaminated industrial effluents," World J Microbiol Biotechnol., vol. 21, pp. 815-822, 2005.
[22] S. S. Yazdani and R. Gonzalez, "Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry," Curr Opin Biotechnol., vol. 18, pp. 213-219, 2007.
[23] Thai Industrial Standards Institute, "Standard for crude glycerine," TIS 336-2538, Ministry of Industry, Bangkok, Thailand, 1995, pp. 1-47.
[24] K. Rosenwinkel and H.Meyer, "Anaerobic treatment of slaughterhouse residues in municipal digesters," Water Sci Technol., vol. 40, pp. 101- 111, 1999.
[25] D. H. Bergey and G. H. John, "Bergey-s manual of determinative Bacteriology," William and Wilkins, Baltimore, Maryland, 1994, pp. 71- 74.
[26] M. P. Prasad and K. Manjunath, "Comparative study on biodegradation of lipid-rich wastewater using lipase producing bacterial species," Indian J Biotechnol., vol. 10, pp. 121-124, 2011.
[27] A. A. Mendes, H. F. Castro, E. B. Pereira, and A. Furigo Jr, "Application of lipases for wastewater treatment containing high levels of lipids," Quim Nova., vol. 28, pp. 296-305, 2005.
[28] M. C. M. R. Leal, D. M.G. Freire, M. C. Cammarota, G. L. Sant-Anna Jr, "Effect of enzymatic hydrolysis on anaerobic treatment of dairy wastewater," Process Biochem., vol. 41, pp. 1173-1178, 2006.
[29] B. A. Adelekan and A. I. Bamgboye, "Comparison of biogas productivity of cassava peels mixed in selected ratios with major livestock waste types," African J Agric Res., vol. 4, pp. 571-577, 2009.
[30] M. J. Broughton, J. H. Thiele, E. J. Birch, and A. Cohem, "Anaerobic batch digestion of sheep tallow," Water Res., vol. 32, pp. 1423-1428, 1998.
[31] S. Luostarinen, S. Luste, and M. Sillanpaa, "Increased biogas production at wastewater treatment plants through co-digestion of sewage sludge with grease trap sludge from a meat processing plant," Bioresour Technol., vol. 100, pp. 79-85, 2009.
[32] G. S. Geeta, C. V. Suvarna, and K. S. Jagdeesh, "Enhanced methane production by sugarcane trash pretreated with Phanerochaete chrysosporium," J Microbiol Biotechnol., vol. 9, pp. 113-117, 1994.