Characterization of Penicillin V Acid and Its Related Compounds by HPLC
Background: 'Penicillin V' is a narrow, bactericidal antibiotic of the beta-lactam family of the naturally occurring penicillin group. It is limited to infections due to the germs defined as sensitive. The objective of this work was to identify and to characterize Penicillin V acid and its related compounds by High-performance liquid chromatography (HPLC). Methods: Firstly phenoxymethylpenicillin was identified by an infrared absorption. The organoleptic characteristics, pH, and determination of water content were also studied. The dosage of Penicillin V acid active substance and the determination of its related compounds were carried on waters HPLC, equipped with a UV detector at 254 nm and Discovery HS C18 column (250 mm X 4.6 mm X 5 µm) which is maintained at room temperature. The flow rate was about 1 ml per min. A mixture of water, acetonitrile and acetic acid (65:35:01) was used as mobile phase for phenoxyacetic acid ‘impurity B' and a mixture of water, acetonitrile and acetic acid (650:150:5.75) for the assay and 4-hydroxypenicillin V 'impurity D'. Results: The identification of Penicillin V acid active substance and the evaluation of its chemical quality showed conformity with USP 35th edition. The Penicillin V acid content in the raw material is equal to 1692.22 UI/mg. The percentage content of phenoxyacetic acid and 4-hydroxypenicillin V was respectively: 0.035% and 0.323%. Conclusion: Through these results, we can conclude that the Penicillin V acid active substance tested is of good physicochemical quality.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1315567Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 553
 André Bryskier. Antibiotique Agent Antibactériens et Antifongiques. Ellipses 1999.
 European Pharmacopoeia 8th edition.
 United States Pharmacopoeia Convention: United States Pharmacopoeia 35.
 Bertram G Katzung. Pharmacologie fondamentale et clinique. Traduit par la la direction de Georges Lagier Paris le 23 Avril 2000.
 D Lebrun, M Bonnet, A Limelette, C de Champs. Pénicillines et inhibiteurs de bétalactamases. Elsevier Masson SAS 2017.