Formation of Vasoactive Amines in Dry Fermented Sausage Petrovská Klobása during Drying and Ripening in Traditional and Industrial Conditions

Authors: Tatjana A. Tasić, Predrag M. Ikonić, Ljiljana S. Petrović, Marija R. Jokanović, Vladimir M. Tomović, Branislav V. Šojić, Snežana B. Škaljac

Abstract:

Formation of histamine, tryptamine, phenylethylamine and tyramine (vasoactive amines) in dry fermented sausage Petrovská klobása during drying and ripening in traditional room (B1) and industrial ripening chamber (B3) were investigated. Dansyl chloride derivatized vasoactive amines were determined using HPLC-DAD on Eclipse XDB-C18 column.

Histamine, the most important amine from food safety point of view, was not detected in any analyzed sample. Unlike most of the other fermented sausages, where tyramine is reported as the most abundant amine, in Petrovská klobása tryptamine was the most abundant vasoactive amine in both groups of sausages even though concentrations of tryptamine and tyramine in B3 sausages at the end of ripening were nearly the same (39.8 versus 39.6mg/kg). Sum of vasoactive amines in samples varied from not detected ND (B3) to 176 mg/kg (B1), with concentration of 36.1 (B3) and 73.6 (B1) mg/kg at the end of drying and 96 (B3) and 176 (B1) mg/kg at the end of ripening period. Although the sum of vasoactive amines has increased from the end of drying (45. and 90. day) to the end of ripening period (120. day), during whole production period these values did not exceed 200 mg/kg proposed as possible indicator of hygienic conditions and GMP in the sausage production.

Keywords: Vasoactive amines, traditional dry fermented sausage Petrovská klobása.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1092209

References:

[1] M. H. S. Santos "Biogenic amines: their importance in foods”, Int. J. Food Microbiol. vol. 29, pp. 213–231, 1996.
[2] M. L. Latorre-Moratalla, T. Veciana-Nogués, S. Bover-Cid, M. Garriga, T. Aymerich, E. Zanardi, A. Ianieri, M.J. Fraqueza, L. Patarata, E.H. Drosinos, A. Lauková, R. Talon, M. C Vidal-Carou, "Biogenic amines in traditional fermented sausages produced in selected European countries”, Food Chem., vol. 107, pp. 912–921, 2008.
[3] M. C. Vidal-Carou, M. L. Izquierdo-Pulido, M. C. Martin-Morro, A. Mariné-Font, "Histamine and tyramine in meat products: Relationship with meat spoilage”, Food Chem., vol. 37, pp. 239–249. 1990.
[4] S. Bover-Cid, M. Izquierdo-Pulido, M. C. Vidal-Carou, "Effect of the intercation between a low tyramine-producing Lactobacillus and proteolitic staphylococci on biogenic amine production during ripening and storage of dry sausages”, Int. J. Food Microbiol., vol. 65, pp.113–123, 2001.
[5] M. L. Latorre-Moratalla, S. Bover-Cid, R. Talon, M. Garriga, E. Zanardi, A. Ianieri, M.J. Fraqueza, M. Elias, E.H. Drosinos, M.C. Vidal-Carou, "Strategies to reduce biogenic amine accumulation in traditional sausage manufacturing”, LWT-Food Science and Technology, vol. 43, pp 20-25, 2010.
[6] H. S Eerola, A. X Roig Sagues, T. K. Hirvi. "Biogenic amines in finnish dry sausages”, J of Food Safety, vol. 18, pp 127–138, 1998.
[7] G. Suzzi, F. Gardini, "Biogenic amines in dry fermented sausages: a review”, Int. J. Food Microbiolo, vol 88, pp 41–54, 2003.
[8] T. Hernndez-Jover, M. Izquierdo-Pulido, M. T. Veciana-Nogus, A. Marin-Font, M. C. Vidal- Carou, "Biogenic Amine and Polyamine Contents in Meat and Meat Products”, J. .Agric. Food Chem, vol 45, pp 2098–2102, 1997.
[9] J. E. Stratton, R. V. Hutkins, S. L. Taylor, "Biogenic amines in cheese and other fermented foods. A rewiew”, Journal of Food Protection, vol 54, pp 460-470, 1991.
[10] EC 2005. Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs. Official Journal of the European Union, L388: pp. 1-26.
[11] Lj. Petrović, N. Džinić, V. Tomović, P. Ikonić, T. Tasić, "Registrated geographical indications ''Petrovská klobása'' for dry fermented sausagesas PDO under Serbian legislation”. Department of intelectual property, Republic of Serbia. 2007, Decision No. 9652/06 Г-03/06.
[12] P. Ikonić, Lj. Petrović, T. Tasić, N. Džinić, M. Jokanović, V. Tomović, "Physicochemical, biochemical and sensory properties for the characterization of Petrovská klobása (traditional fermented sausage)” Acta periodica technologica. vol 41, pp 19–31, 2010.
[13] Lj. Petrović, N. Džinić, P. Ikonić, T. Tasić, V. Tomović, "Quality and safety standardization of traditional fermented sausages”, Tehnologija mesa, vol 2, pp 234-244, 2011.
[14] A. R. Shalaby, "Significance of biogenic amines to food safety and human health”, Food Res. Int., vol 29, pp 675–690, 1996.
[15] Serbian Regulations 31/2012, in Službeni list, Beograd, SCG 31, 2012.
[16] S. Eerola, R. Hinkkanen, E. Lindfors, T. Hirvi, "Liquid chromatographic determination of biogenic amines in dry sausages”, J. AOAC Int, vol 76, pp 575–577; 1993.
[17] T. Tasić, P. Ikonić, A. Mandić, M. Jokanović, V. Tomović, S. Savatić, Lj. Petrović, "Biogenic amines content in traditional dry fermented sausage Petrovská klobása as possible indicator of good manufacturing practice”, Food Cont., vol. 23, no. 1, pp. 107–112, 2012.
[18] E. Parente, M. Martuscelli, F. Gardini, S. Grieco, M. A. Crudele, G Suzzi,. "Evolution of microbial populations and biogenic amine production in dry sausages produced in Southern Italy”, J. .Appl. Microbiol., vol 90, pp 882–891, 2001.
[19] T. Komprda, D. Smĕlá, P. Pechová, L. Kalhotka, J. Štencl, B. Klejdus, "Effect of starter culture, spice mix and storage time and temperature on biogenic amine content of dry fermented sausages”, Meat Sci., vol 67, pp 607–616, 2004.
[20] I. Lebert, S. Leroy, P. Giammarinaro, A. Lebert, J.P. Chacornac, S. Bover-Cid, M.C. Vidal-Carou, R Talon, "Diversity of microorganisms in the environment and dry fermented sausages of small traditional French processing units”, Meat Sci., vol 76, pp 112–122, 2007.