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Isolation of Biosurfactant Producing Spore-Forming Bacteria from Oman: Potential Applications in Bioremediation

Authors: Saif N. Al-Bahry, Yahya M. Al-Wahaibi, Abdulkadir E. Elshafie, Ali S. Al-Bemani, Sanket J. Joshi

Abstract:

Environmental pollution is a global problem and best possible solution is identifying and utilizing native microorganisms. One possible application of microbial product -biosurfactant is in bioremediation of hydrocarbon contaminated sites. We have screened forty two different petroleum contaminated sites from Oman, for biosurfactant producing spore-forming bacterial isolates. Initial screening showed that out of 42 soil samples, three showed reduction in surface tension (ST) and interfacial tension (IFT) within 24h of incubation at 40°C. Out of those 3 soil samples, one was further selected for isolation of bacteria and 14 different bacteria were isolated in pure form. Of those 14 spore-forming, rod shaped bacteria, two showed highest reduction in ST and IFT in the range of 70mN/m to <35mN/m and 26.69mN/m to <9mN/m, respectively within 24h. These bacterial biosurfactants may be utilized for bioremediation of oil-spills.

Keywords: Bioremediation, hydrocarbon pollution, spore-forming bacteria, biosurfactant

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1090575

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References:


[1] I. M. Banat, "Biosurfactants production and possible uses in microbial enhanced oil recovery and oil pollution remediation: a review," Bioresour. Technol. 51, pp. 1-12, 1995.
[2] C. N. Mulligana, R. N. Yongb, and B. F. Gibbsc, "Surfactant-enhanced remediation of contaminated soil: a review," Eng Geology, vol. 60, pp. 371-380, 2001.
[3] S J. Joshi and A. J. Desai, "Biosurfactants: Role in bioremediation of NAPL & fermentative production," In: Biosurfactants, (Ed. R. Sen), 2010, Springer and Landes Bioscience publications, USA, pp. 222-235.
[4] J. D. Desai, and I. M. Banat, "Microbial production of surfactants and their commercial potential," Microbiol. Mol. Biol. Rev., vol. 61, pp. 47–64, 1997.
[5] S. J. Joshi, H.Suthar, A. K. Yadav, K. Hingurao, and A. Nerurkar, "Occurrence of Biosurfactant Producing Bacillus spp. in Diverse Habitats," ISRN Biotechnol, vol. 2013, 2012.
[6] D. G. Cooper, C. R. Macdonald, S. J. B. Duff, and N.Kosaric, "Enhanced production of surfactin from Bacillus subtilis by continuous product removal and metal cation addition," Appl. Environ. Microbiol. Vol. 42, pp. 408–412, 1981.
[7] H. Al-Sulaimani, Y. Al-Wahaibi, S.N. Al-Bahry, A. Elshafie, A. Al-Bemani, S. Joshi and S. Zaragari, "Optimization and partial characterization of biosurfactant produced by Bacillus species and their potential for enhanced oil recovery," SPEJ, vol. 1, pp. 672-682, 2011.
[8] H. Al-Sulaimani, Y. Al-Wahaibi, S.N. Al-Bahry, A. Elshafie, A. Al-Bemani, S. Joshi and S. Zaragari, "Experimental investigation of biosurfactants produced by Bacillus species and their potential for MEOR in Omani oil field," SPE 129228, 2010.
[9] Al-Sulaimani, H., S. Joshi, Y. Al-Wahaibi, S. Al-Bahry, A. Elshafie, and A. Al-Bemani. "Microbial biotechnology for enhancing oil recovery: Current developments and future prospects." Biotechnol. Bioinf. Bioeng,vol. 1,pp. 147-158, 2011.
[10] M. Javaheri, G. E. Jenneman, M. J. McInerney, and R. M. Knapp, "Anaerobic production of a biosurfactant by Bacillus licheniformis JF-2,” Appl. Env. Microbiol., vol. 50, pp. 698–700, 1985.
[11] M. Nitschke, and G. M.Pastore, "Production and properties of a surfactant obtained from Bacillus subtilis grown on cassava wastewater," Bioresour. Technol., vol. 97, pp. 336-341, 2006.