Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30172
PCR based Detection of Food Borne Pathogens

Authors: Archana Panchapakesan Iyer, Taha Abdullah Kumosani

Abstract:

Many high-risk pathogens that cause disease in humans are transmitted through various food items. Food-borne disease constitutes a major public health problem. Assessment of the quality and safety of foods is important in human health. Rapid and easy detection of pathogenic organisms will facilitate precautionary measures to maintain healthy food. The Polymerase Chain Reaction (PCR) is a handy tool for rapid detection of low numbers of bacteria. We have designed gene specific primers for most common food borne pathogens such as Staphylococci, Salmonella and E.coli. Bacteria were isolated from food samples of various food outlets and identified using gene specific PCRs. We identified Staphylococci, Salmonella and E.coli O157 using gene specific primers by rapid and direct PCR technique in various food samples. This study helps us in getting a complete picture of the various pathogens that threaten to cause and spread food borne diseases and it would also enable establishment of a routine procedure and methodology for rapid identification of food borne bacteria using the rapid technique of direct PCR. This study will also enable us to judge the efficiency of present food safety steps taken by food manufacturers and exporters.

Keywords: food borne pathogens, PCR, food safety, rapiddetection.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1329440

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2331

References:


[1] Jaykus, L.-A. Challenges to developing real-time methods to detect pathogens in foods. 2003 ASM News. 69, pp.341-347.
[2] Fode-Vaughan, K.A., Wimpee, C.F., Remsen, C.C. and Collins,M.L.P. Detection of bacteria in environmental samples by direct PCR without DNA extraction.2001. Biotechniques 31, pp.598-607.
[3] Naravaneni, R & Jamil K. Rapid detection of food borne pathogens by using molecular techniques.2005. Journal of Medical Microbiology. 54, pp 51-54.
[4] Swee-Han Goh, Sean K.Byrne, J.L.Zhang & Anthony W.Chow. Molecular Typing of Staphylococcus aureus on the basis of coagulase Gene Polymorphisms.1992. Journal of Clinical Microbiology, pp. 1642- 1645.
[5] Kazuhisa Murakami, Wakio Minamide, Koji Wada, Etuo Nakamura, Hiroshi Teraoka & Sachihiko Watanabe. Identification of Methicillin- Resistant Strains of Staphylococci by Polymerase Chain Reaction. 1991.Journal of Clinical Microbiology, pp.2240-2244.
[6] Fode-Vaughan K.A, Maki J.S, Benson J.A & Collins M.L.P. Direct PCR detection of Escherichia coli O157:H7.2003. Letters in Applied Microbiology , 37, pp. 239-243.