Commenced in January 2007
Paper Count: 32146
Statistical Optimization of Process Variables for Direct Fermentation of 226 White Rose Tapioca Stem to Ethanol by Fusarium oxysporum
Abstract:Direct fermentation of 226 white rose tapioca stem to ethanol by Fusarium oxysporum was studied in a batch reactor. Fermentation of ethanol can be achieved by sequential pretreatment using dilute acid and dilute alkali solutions using 100 mesh tapioca stem particles. The quantitative effects of substrate concentration, pH and temperature on ethanol concentration were optimized using a full factorial central composite design experiment. The optimum process conditions were then obtained using response surface methodology. The quadratic model indicated that substrate concentration of 33g/l, pH 5.52 and a temperature of 30.13oC were found to be optimum for maximum ethanol concentration of 8.64g/l. The predicted optimum process conditions obtained using response surface methodology was verified through confirmatory experiments. Leudeking-piret model was used to study the product formation kinetics for the production of ethanol and the model parameters were evaluated using experimental data.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1070625Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1484
 Moiser, N., C. Wyman, B. Dake, R. Elander, Y. Y. Lee, and M. Holtzapple, 2005, "Features of promising technologies for pre-treatment of lignocellulosic biomass", Bioresource Technology, 96, 673 - 686.
 Jian Shi, Ratna R. Sharma-Shivappa, Mari Chinn, Noura Howell, 2008, "Effect of microbial pre-treatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production", Biomass and Bioenergy, 33, 88 - 96.
 Gianni Panagiotou, Paul Christakopoulos, S. G. Villas-Boas, L. Olsson, 2005, "Fermentation performance and intracellular metabolite profiling of Fusarium oxysporum cultivated on a glucose-xylose mixture", Enzyme and Microbial Technology, 36, 100-106.
 Paul Christakopoulos, Basil J. Macris, Dimitris Kekos, 1990, "On the mechanisms of direct conversion of cellulose to ethanol by Fusarium oxysporum: effect of cellulose and β-glucosidase" Applied Microbiology and Biotechnology, 33, 18-20.
 Christakopoulos, P., D. Mamma, D. Kekos, 1999, "Enhanced acetyl esterase production by Fusarium oxysporum" World Journal of Microbiology and Biotechnology, 15, 443-446.
 Panagiotou, G., Christakopoulos, P., Olsson, L. (2005) Simultaneous saccharification and fermentation of cellulose by Fusarium oxysporum F3- growth characteristics and metabolite profiling", Enzyme and Microbial Technology, 36, 693-699.
 Box, G. E., and N. R. Draper, 1987, "Empirical model building and response surfaces", Wiley, New york.
 Mason, R. I., R. F. Gunst, and J. L. Hess,. 1989, "Statistical design and analysis of experiments" Wiley, New york.
 Khuri, A. I., and J. A. Cornell, 1987, "Response Surfaces: Design and Analysis", Marcel Dekker, New York.
 Montgomery, D. C., 1991, "Design and Analysis of experiments", 3rd edn, Wiley, New York.
 Chang, V., and M. Holtzapple, 2000, "Fundamental factors affecting biomass enzymatic reactivity", Applied Biochemistry and Biotechnology, 84-86, 5-36.
 Sadasivam, S., and A. Manickam, 1996, "Biochemical Methods" 2nd edn.
 Bailey, J. E., and D. F. Ollis, 1986, "Biochemical Engineering Fundamentals", McGraw-Hill, New York.
 Cowling, E. B., 1975, "Physical and chemical constraints in the hydrolysis of cellulose and lignocellulosic materials", Biotechnology and Bioengineering Symposium, 5, 163 - 81.
 Mala Rao, Chittra Mishra, Sulbha Keskar, M. C. Srinivasan, 1985, "Production of ethanol from wood and agricultural residues by Neurospora crassa", Enzyme and Microbial Technology, 7, 625-628.
 Vasanti Deshpande, Sulbha Keskar, Chitra Mishra and Mala Rao, 1986, "Direct conversion of cellulose/hemicellulose to ethanol by Neurospora crassa", Enzyme and Microbial Technology, 8, 149-152.