Evaluation of the Immunoregulatory Activity of rFip-gts Purified from Baculovirus-infected Insect Cells
Fip-gts, an immunomodulatory protein purified from Ganoderma tsugae, has been reported to possess therapeutic effects in the treatment of cancer and autoimmune disease. For medicinal application, a recombinant Fip-gts was successfully expressed and purified in Sf21 insect cells by our previously work. It is important to evaluate the immunomodulatory activity of the rFip-gts. To assess the immunomodulatory potential of rFip-gts, the T lymphocytes of murine splenocytes were used in the present study. Results revealed that rFip-gts induced cellular aggregation formation. Additionally, the expression of IL-2 and IFN-r were up-regulated after the treatment of rFip-gts, and a corresponding increased production of IL-2 and IFN-r in a dose-dependent manner. The results showed that rFip-gts has an immunomodulatory activity in inducing Th1 lymphocytes from murine splenocytes released IL-2 and IFN-γ, thus suggest that rFip-gts may have therapeutic potential in vivo as an immune modulator.
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 Chiang Su New Medical College, "Dictionary of Chinese crude drug Shanghai Scientific Technological Publishers," Shanghai, Chinese, 1979, pp.16.
 W. H. Lin, C. H. Hung, C. I. Hsu, and J.Y. Lin, "Dimerization of the N-terminal amphipathic alpha-helix domain of the fungal immunomodulatory protein from Ganoderma tsugae (Fip-gts) defined by a yeast two-hybrid system and site-directed mutagenesis," J Biol Chem, vol.272, pp. 20044-20048, Aug. 1997.
 S. Tanaka, K. Ko, K. Kino, K. Tsuchiya, A. Yamashita, A. Murasugi, S. Sakuma, and H. Tsunoo, "Complete amino acid sequence of an immunomodulatory protein, ling zhi-8 (LZ-8)," J Biol Chem, vol. 264, pp.16372-16377, Oct. 1989.
 K. Kino, A. Yamashita, K. Yamaoka, J. Watanabe, S. Tanaka, K. Ko, K. Shimizu, and H. Tsunoo, "Isolation and characterization of a new immunomodulatory protein, ling zhi-8 (LZ-8), from Ganoderma lucidium," J Biol Chem, vol. 264, pp. 472-478, Jan. 1989.
 N. S. Lai, R. H. Lin, R. S. Lai, U. C. Kun, S. C. Leu, "Prevention of autoantibody tormation and prolonged survival in New Zealand Black/New Zealand white F1 mice with anancient hinese herb, Ganoderma tsugae," Lupus., vol. 10, pp. 461-465, 2001
 C. H. Liao, Y. M. Hsiao, C. P. Hsu, M. Y. Lin, J. C. Wang, Y. L. Huang, and J. L. Ko, "Transcriptionally mediated inhibition of telomerase of fungal immunomodulatory protein from Ganoderma tsugae in A549 human lung adenocarcinoma cell line," Mol. Carcinog., vol. 45, pp. 220-229, Apr. 2006.
 C. H. Liao, Y. M. Hsiao, C. H. Lin, C. S. Yeh, J. C. Wang, C. H. Ni, C. P. Hsu, and J. L. Ko, "Induction of premature senescence in human lung cancer by fungal immunomodulatory protein from Ganoderma tsugae," Food. Chem. Toxicol., vol. 46, pp. 1851-1859, May. 2008.
 P. H. Wang, S. F. Yang, G. D. Chen, C. P. Han, S. C. Chen, L. Y. Lin, and J. L .Ko, "Human nonmetastatic clone 23 type 1 gene suppress migration of cervical cancer cells and enhances the migration inhibition of fungal immunomodulatory protein from Ganoderma tsugae," Reprod. Sci., vol. 14, pp. 475-485, Jul. 2009.
 T. R .Jinn, C. M. Wu, W. C. Tu, J. L. Ko, and Jason T. C. Tzen "Functional expression of Fip-gts, a fungal immunomodulatory protein from Ganoderma tsugae in Sf21 insect cells," Biosci. Biotechnol. Biochem., vol. 70, pp. 2627-2634, Nov. 2006.
 T. Y. Wu, C. M. Wu, J. L. Ko, T .R. Jinn, "Efficient production of the immunomodulatory glycoprotein Fip-gts from Ganoderma tsugae in baculovirus-infected insect larvae," Biosci. Biotechnol. Biochem., submitted for publication.
 C. L. C. Giraudo, L. Rumi, and C. D. Pasqualini, "An improved method for PHA-culturing mouse lymphocytes using Ficoll-Hypaque gradient," Boll. Ist. Sieroter. Milan, vol. 55, pp.304-307, Sep. 1976.
 A. S. Wermerskirchen, D. H. LaTocha, and B. L. Clarke, "Adrenocorticotropic hormone controls Concanavalin A activation of rat lymphocytes by modulating IL-2 production," Life Science, vol. 67, pp. 2177-2187, Sep. 2000.