TY - JFULL AU - Tzong Yuan Wu and Sheng Kuo Hsieh and Tzyy Rong Jinn PY - 2010/6/ TI - Evaluation of the Immunoregulatory Activity of rFip-gts Purified from Baculovirus-infected Insect Cells T2 - International Journal of Medical and Health Sciences SP - 204 EP - 207 VL - 4 SN - 1307-6892 UR - https://publications.waset.org/pdf/6918 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 41, 2010 N2 - Fip-gts, an immunomodulatory protein purified from Ganoderma tsugae, has been reported to possess therapeutic effects in the treatment of cancer and autoimmune disease. For medicinal application, a recombinant Fip-gts was successfully expressed and purified in Sf21 insect cells by our previously work. It is important to evaluate the immunomodulatory activity of the rFip-gts. To assess the immunomodulatory potential of rFip-gts, the T lymphocytes of murine splenocytes were used in the present study. Results revealed that rFip-gts induced cellular aggregation formation. Additionally, the expression of IL-2 and IFN-r were up-regulated after the treatment of rFip-gts, and a corresponding increased production of IL-2 and IFN-r in a dose-dependent manner. The results showed that rFip-gts has an immunomodulatory activity in inducing Th1 lymphocytes from murine splenocytes released IL-2 and IFN-γ, thus suggest that rFip-gts may have therapeutic potential in vivo as an immune modulator. ER -