Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 31532
Effect of Fermentation Time on Xanthan Gum Production from Sugar Beet Molasses

Authors: Marzieh Moosavi- Nasab, Safoora Pashangeh, Maryam Rafsanjani


Xanthan gum is a microbial polysaccharide of great commercial significance. The purpose of this study was to select the optimum fermentation time for xanthan gum production by Xanthomonas campestris (NRRL-B-1459) using 10% sugar beet molasses as a carbon source. The pre-heating of sugar beet molasses and the supplementation of the medium were investigated in order to improve xanthan gum production. Maximum xanthan gum production in fermentation media (9.02 g/l) was observed after 4 days shaking incubation at 25°C and 240 rpm agitation speed. A solution of 10% sucrose was used as a control medium. Results indicated that the optimum period for xanthan gum production in this condition was 4 days.

Keywords: Biomass, Molasses, Xanthan gum, Xanthomonascampestris

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3421


[1] A. Margaritis, J. E. Zajic, "Biotechnology review: mixing mass transfer and scale-up of polysaccharide fermentations,". Vol. 20. Biotechnol Bioeng, 1978, pp. 939-1001.
[2] E. B. Kurbanoglu, N. I. Kurbanoglu, "Ram horn hydrolysate as enhancer of xanthan production in batch culture of Xanthomonas campestris EBK- 4 isolate,". Vol. 42. Process Biochemistry, 2007, pp. 1146-1149.
[3] F. Garc├Á├éa-Ochoa, V. E. Santos, J. A. Casas, E. Go├émez, "Xanthan gum: production, recovery, and properties,". Vol. 18. Biotechnology Advances, 2000, pp. 549-579.
[4] M. C. Cadmus, C. A. Knutson, "Production of high-pyruvate xanthan gum on synthetic medium," . Vol. 4. US Patent, 1983, pp. 394-447.
[5] M. Papagianni, S. K. Psomas, L. Batsilas, S. V. Paras, D. A. Kyriakidis, M. Liakopoulou-Kyriakides, "Xanthan production by Xanthomonas campestris in batch cultures,". Vol. 37. Process Biochem, 2001, pp. 73- 80.
[6] P. S. Gils, D. Ray, P. K. Sahoo, "Characteristics of xanthan gum-based biodegradable superporous hydrogenl,". Vol. 45. International Journal of Biological Macromolecules, 2009. 45, 364-371.
[7] S. Kalogiannis, G. Iakovidou, M. Liakopoulou-Kyriakides, D. A. Kyriakidis, G. N. Skaracis, "Optimization of xanthan gum production by Xanthomonas campestris grown in molasses,". Vol. 39. Process Biochemistry, 2003. 249-256.
[8] S. Rosalam, R. England, "Review of xanthan gum production from unmodified starches by Xanthomonas comprestris sp,". Vol. 39. Enzyme and Microbial Technology, 2006, pp. 197-207.
[9] S. D. Yoo, S.W. Harcum, "Xanthan gum production from waste sugar beet pulp,". Vol. 70. Bioresource Technology, 1999, pp. 105-109.
[10] S. N. Yuen, S. M. Choi, D. L. Phillips, C. Y. Ma, "Raman and FTIR spectroscopic study of carboxymethylated non-starch polysaccharides,". Vol. 114. Food Chemistry, 2009, pp. 1091-1098.