Optimization of Extraction of Phenolic Compounds from Avicennia marina (Forssk.)Vierh using Response Surface Methodology

Authors: V.Bharathi, Jamila Patterson, R.Rajendiran

Abstract:

Optimization of extraction of phenolic compounds from Avicennia marina using response surface methodology was carried out during the present study. Five levels, three factors rotatable design (CCRD) was utilized to examine the optimum combination of extraction variables based on the TPC of Avicennia marina leaves. The best combination of response function was 78.41 °C, drying temperature; 26.18°C; extraction temperature and 36.53 minutes of extraction time. However, the procedure can be promptly extended to the study of several others pharmaceutical processes like purification of bioactive substances, drying of extracts and development of the pharmaceutical dosage forms for the benefit of consumers.

Keywords: Avicennia marina, Central Composite RotatableDesign (CCRD), Response Surface Methodology, Total Phenoliccontents (TPC)

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1056444

References:

[1] P. B. Tomlinson, The Botany of Mangroves, Cambridge University Press. New York, USA, 163-170, 1994.
[2] W. M. Bandarnayake, "Bioactivities, bioactive compounds and chemical constituents of mangrove plants", Wetlands Ecol. Manage, 10: 421-452, 2002.
[3] D. H. Miles, U. Kokpol, V. Chittawong, S. Tip-Pyang, K. Tunsuwan, and C. Nguyen, "Mangrove forests-The importance of conservation as a bioresource for ecosystem diversity and utilization as a source of chemical constituents with potential medicinal and agricultural value", 1999 IUPAC 70 (11): 1-9, 1998.
[4] P. Maisuthisakul, M. Suttajit, and R. Pongsawatmanit, "Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants", Food Chem., 100: 1409-1418, 2007.
[5] C. Vioux-Chagnoleau, F. Lejeune, J. Sok, C. Pierrard, C. Marionnet, and F. Bernerd, "Reconstructed human skin: from photodamage to sunscreen photoprotection and anti-aging molecules", J. Dermatol. Sci. (Supplement), 2(1): S1-S12, 2006.
[6] S. Surveswaran, Y. Z. Cai, H. Corke, and M. Sun, "Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants", Journal of Food Chemistry 102: 938-953, 2007.
[7] C. Alasalvar, M. Karamac, R. Amarowicz, and F. Shahidi, "Antioxidant and Antiradical Activities in Extracts of Hazelnut Kernel (Corylus avellana L.) And Hazelnut Green Leafy Cover", Journal of Agriculture and Food Chemistry 54: 4826-4832, 2006.
[8] E. A. Audi, E. J. V. Campos, M. Rufino, D.G. Cortez, C. A. Bersani- Amado, L. A. L. Soares, P. R. Petrovick, and J. C. P. Mello, "Petiveria alliacea L.: Plant drug quality control, hydroalcoholic extract standardization and pharmacological assay of lyophilized extract", Acta Farmaceutica Bonaerense, 20 (3), 225-232, 2001.
[9] W. Cui, G. Mazza, B. D. Oomah, and C. G. Billiaderis, "Optimization of anaqueous extraction process for flaxseed gum by response surface methodology", Lebensmittel-Wissenschaft und-Technologie, 27, 363- 369, 1994.
[10] P.O. Haaland, Experimental Design in Biotechnology, Marcel Dekker, New York. PP. 1-18, 1989.
[11] G.E.P. Box, W.G. Hunter, and J.S. Hunter, Statistics for Experimenters: an introduction to design, data analysis, and model building, New York, John Wiley, 1978.
[12] R. H. Myers, and D. C. Montgomery, "Response surface methodology, process and product optimization using designed experiments (2nd Ed.). New York, NY: John Wiley and Sons, 1995.
[13] M. H. Canteri-Schemin, H. C. R. Fertonani, N. Waszczynskyj, and G. Wosiacki, "Extraction of pectin from apple pomace", Brazilian Archives of Biology and Technology, 48 (2), 259-266, 2005.
[14] L. A. L. Soares, G. González Ortega, P. R. Petrovick, and P. C. Schmidt, "Optimization of Tablets Containing a High Dose of Spray-Dried Plant Extract: A Technical Note. AAPSPharmSciTech 6(3), 368-371, 2005.
[15] Y.Y. Lim, T. T. Lim, and J. J. Tee, "Antioxidant properties of several tropical fruits: A comparative studies", Food Chemistry 103: 1003-1008, 2007.
[16] R. H. Myers, and D. C. Montgomery, "Response surface methodology: Process and product optimization using designed experiments," p. 32. New York, USA: Wiley, 2002.
[17] N. Peatciyammal, B. Balachandar, M. Dinesh Kumar, K. Tamilarasan, and C. Muthukumaran, "Statistical Optimization of Enzymatic Hydrolysis of Potato (Solanum tuberosum) Starch by Immobilized ╬▒- amylase", International Journal of Chemical and Biological Engineering, 3:3, 2010.
[18] A.M. Joglekar, and A.T. May, "Product excellence through design of experiments", Cereal Food World, 32: 857-868, 1987.