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Interaction Effect of DGAT1 and Composite Genotype of Beta-Kappa Casein on Economic Milk Production Traits in Crossbred Holstein

Authors: A. Molee, N. Duanghaklang, P. Mernkrathoke


The objective was to determine the single gene and interaction effect of composite genotype of beta-kappa casein and DGAT1 gene on milk yield (MY) and milk composition, content of milk fat (%FAT), milk protein (%PRO), solid not fat (%SNF), and total solid (%TS) in crossbred Holstein cows. Two hundred and thirty- one cows were genotyped with PCR-RFLP for DGAT1 and composite genotype data of beta-kappa casein from previous work were used. Two model, (1), and (2), was used to estimate single gene effect, and interaction effect on the traits, respectively. The significance of interaction effects on all traits were detected. Most traits have consistent pattern of significant when model (1), and (2) were compared, except the effect of composite genotype of betakappa casein on %FAT, and the effect of DGAT1 on MY, which the significant difference was detected in only model (1).The results suggested that when the optimum of all traits was necessary, interaction effect should be concerned.

Keywords: composite genotype of beta-kappa casein, DGAT1gene, Milk composition, Milk yield

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[1] Amonrat, M., L. Boonek, and N. Rungsakinnin. 2011. The Effect of beta and kappa casein genes on milk yield and milk composition in different percentages of holstein in crossbred dairy cattle. Anmal Sci. J. (to be published).
[2] Banos, G., J.A. Woolliams, B.W. Woodward, A.B. Forbes, and M.P. Coffey. 2008. Impact of single nucleotide polymorphisms in leptin, leptin receptor, growth hormone receptor, and diacylglycerol acyltransferase (DGAT1) Gene loci on milk production, feed, and body energy traits of UK dairy cows. J. Dairy Sci. 91: 3190 - 3200.
[3] Bobe, G., D.C. Beitz, A.E. Freeman, and G.L. Linderg. 1999. Effect of milk protein genotypes on milk composition and its genetic parameter estimates. J. Dairy Sci. 82, 2797-2804.
[4] Braunschweig, M., C. Hagger, G. Stanzinger, and Z. Puhan. 2000. Associations between casein haplotypes and milk production traits of Swiss Brown cattle. J Dairy Sci. 83, 1387 - 1395.
[5] Comin, A., M. Cassandro, S. Chessa, M. Ojala, R. Dal Zotto, M. De Marchi, P. Carnier, and L. Gallo. 2008. Effect of composite β and ╬║ casein genotype on milk coagulation, quality and yield traits in Italian Holstein cows. J. Dairy Sci. 91, 4022-4027.
[6] Grisart, B., W. Coppieters, F. Farnir, L. Karim, C. Ford, P. Berzi, N. Cambisano, M. Mni, S. Reid, P. Simon, R. Spelman, M. Georges, and R. Snell. 2002. Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Research, 12(2), 222-231.
[7] Ikonen, T., M. Ojala, and O. Ruottinen, 1999. Associations between milk protein polymorphism and first lactation milk production traits in Finnish Ayrshire cows. J. Dairy Sci. 82, 1026-1033.
[8] Jann, O. C., E. M. Ibeagha-Awemu, Ozbeyaz, C. Zaragoza, P. Williams, J. L. Ajmone-Marsan, P. Lenstra, J. A. Moazami-Goudarzi, and K. Erardt. 2004. Geographic distribution of haplotype diversity at the bovine casein locus. Genet. Sel. Evol. 36, 243-257.
[9] Khatib, H., W. Huang, X. Wang, A.H. Tran, A.B. Bindrim, V. Schutzkus, R.L. Monson, and B.S. Yandell. 2009. Single gene and gene interaction effects on fertilization and embryonic survival rates in cattle. J. Dairy Sci. 92: 2238 - 2247.
[10] Koczan D., G. Hobom, and H. -M. Seyfert. 1991. Genomic organization of the bovine alpha-s1 casein gene. Nucl. Acids Res. 19, 5591-5596.
[11] Kuehn, C., C. Edel, R. Weikard, G. Thaller. 2007. Dominance and parent-of-origin effects of coding and non-coding alleles at the acylCoA-diacylglycerol-acyltransferase (DGAT1) gene on milk production traits in German Holstein cows. BMC Genetics. 8, 62-70.
[12] Mercier, J. C., and J. L. Viloite. 1993. Structure and function of milk protein genes. J. Dairy Sci. 76, 3079-3098.
[13] Naslund, J., W.F. Fikse, G.R. Pielberg, A. Lunden. 2008. Frequency and effect of the bovine acyl -CoA:diacylglycerol acyltransferase 1 (DGAT1) K232A polymorphism in Swedish dairy cattle. Journal Dairy Science, 91 (5), 2127-2134.
[14] Sanders, K., J. Bennewitz, N. Reinsch, G. Thaller, E-M. Prinzenberg, C. Kuhn, and E .Kalm. 2006. Characterization of the DGAT1 Mutations and the CSN1S1 Promoter in the German Angeln Dairy Cattle Population. J. Dairy Sci. 89: 3164 - 3174.
[15] Signorelli, F, L. Orrù, F. Napolitano, G.D. Matteis, M.C. Scatà, G. Catillo, C. Marchitelli, B. Moioli. 2009. Exploringpolymorphisms and effects on milk traits of the DGAT1, SCD1 and GHR genes in four cattle breeds. Livestock Science, 125, 74-79.
[16] Sun, D., J. Jia, Y. Ma, Y. Zhang, Y. Wang, Y. Yu, and Y. Zhang. 2009 Effects of DGAT1 and GHR on milk yield and milk composition in the Chinese dairy population. Animal Genetics, 40, 997-1000.
[17] Winter, A., W. Kramer, F. Werner, S. Kollers, S. Kata, G. Durstewits, and J. Buitkamp, J.E. Womack, G. Thaller, and R. Fries. 2002. Association of a lysine-232/alanine polymorphism in a bovine gene encoding diacylglycerol acyltransferase (DGAT) with variation at a quantitative trait locus for on milk fat content in cattle. Proceedings of the National Academy of Sciences, 99, 9300-9305.