Highly Sensitive Label Free Biosensor for Tumor Necrosis Factor

Authors: Tze Sian Pui, Tushar Bansal, Patthara Kongsuphol, Sunil K. Arya

Abstract:

We present a label-free biosensor based on electrochemical impedance spectroscopy for the detection of proinflammatory cytokine Tumor Necrosis Factor (TNF-α). Secretion of TNF-α has been correlated to the onset of various diseases including rheumatoid arthritis, Crohn-s disease etc. Gold electrodes were patterned on a silicon substrate and self assembled monolayer of dithiobis-succinimidyl propionate was used to develop the biosensor which achieved a detection limit of ~57fM. A linear relationship was also observed between increasing TNF-α concentrations and chargetransfer resistance within a dynamic range of 1pg/ml – 1ng/ml.

Keywords: Tumor necrosis factor, electrochemical impedance spectroscopy, label free, self assembled monolayer

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1054970

References:

[1] J. Mongkolsapaya et al, Nat Med. 9, 921-927, 2003
[2] World Health Organization, Dengue: guidelines for diagnosis, treatment, prevention and control, 2009
[3] L. Diane et al, Expert Review of Vaccines, 8, 779-789, 2009
[4] J. Jason et al, Clin Diagn Lab Immunol., 8, 1097-1103, 2001
[5] S. S. Khan, M. S. Smith, D. Reda, A. F. Suffredini, J. P. McCoy, 61B, 35-39, 2004
[6] F. Guirakhoo et al, Hum Vaccin., 2, 60-67, 2006
[7] B. Guy B, Vaccine, 26, 5712-5721, 2008
[8] T. G. Connell, PLoS ONE, 3, e2624, 2008
[9] R. Pei, Z. Cheng, E. Wang and X. Yang, Biosensors and Bioelectronics, 16, 355-361, 2001
[10] J. Wang, E. Palecek, P. E. Nielsen, G. Rivas, X. Cai, H. Shiraishi, N. Dontha, D. Luo and P. A. M. Farias, J. Am. Chem. Soc., 118, 7667- 7670, 1996
[11] W. C. I. Homs Anal. Letters, 35, 1875-1894, 2002
[12] A. M. Oliveira-Brett, C. M. A. Brett and L. A. Silva, Bioelectrochemistry, 56, 33-35, 2002
[13] C. M. A. Brett, A. M. O. Brett and S. H. P. Serrano, Electrochimca Acta, 44, 4233-4239, 1999
[14] E. Katz and I. Willner, Electroanalysis, 15, 913-947, 2003
[15] S-M. Park, J-S Yoo, Anal Chem., 75, 455A-461A, 2003
[16] E. Valera, J. R. Azcon, A. Rodriguez, L. M. Castaner, F. J. Sanchez and M. P. Marco, Sensors and Actuators B, 125, 526-537, 2007
[17] J. R. Azcon, E. Valera, A. Rodriguez, A. Barranco, B. Alfaro, F. S. Baeza and M. P. Marco Biosensors and Bioelectronics, 23, 1367-1373, 2008
[18] S. K. Arya, G. Chornokur, M. Venugopal and S. Bhansali, Analyst, 135, 1941-1946, 2010
[19] S. K. Arya, G. Chornokur, M. Venugopal and S. Bhansali, Biosensors and Bioelectronics, 25, 2296-2301, 2010
[20] M. Dijksma, B. Kamp, J. C. Hoogvliet and W. P. Van Bennekom, Anal. Chem., 73, 901-907, 2001
[21] J. T. La Belle et al.,Biosensors and Bioelectronics, 23, 428-431, 2007.
[22] H. Zhu, G. Stybayeva, M. Macal, E. Ramanculov, M. D. George, S. Dandekar and A. Revzin, Lab on chip, 8, 2197-2205, 2008
[23] N. Ganesh, I. D. Block, P. C. Mathias, W. Zhang, E. Chow, V. Malyarchuk and B. T. Cunningham, Optics Express, 16, 21626-21640, 2008
[24] S. Cesaro-Tadic et al, Lab on chip, 4, 563-569, 2004