%0 Journal Article %A Tze Sian Pui and Tushar Bansal and Patthara Kongsuphol and Sunil K. Arya %D 2012 %J International Journal of Biomedical and Biological Engineering %B World Academy of Science, Engineering and Technology %I Open Science Index 69, 2012 %T Highly Sensitive Label Free Biosensor for Tumor Necrosis Factor %U https://publications.waset.org/pdf/1316 %V 69 %X We present a label-free biosensor based on electrochemical impedance spectroscopy for the detection of proinflammatory cytokine Tumor Necrosis Factor (TNF-α). Secretion of TNF-α has been correlated to the onset of various diseases including rheumatoid arthritis, Crohn-s disease etc. Gold electrodes were patterned on a silicon substrate and self assembled monolayer of dithiobis-succinimidyl propionate was used to develop the biosensor which achieved a detection limit of ~57fM. A linear relationship was also observed between increasing TNF-α concentrations and chargetransfer resistance within a dynamic range of 1pg/ml – 1ng/ml. %P 443 - 446