Micro-aerobic, Anaerobic and Two-stage Condition for Ethanol Production by enterobacter aerogenes from Biodiesel-derived Crude Glycerol
The microbial production of ethanol from biodiesel¬derived crude glycerol by Enterobacter aerogenes TISTR1468, under micro-aerobic and anaerobic conditions, was investigated. The experimental results showed that micro-aerobic conditions were more favorable for cellular growth (4.0 g/L DCW), ethanol production (20.7 g/L) as well as the ethanol yield (0.47 g/g glycerol) than anaerobic conditions (1.2 g/L DCW, 6.3 g/L ethanol and 0.72 g/g glycerol, respectively). Crude glycerol (100 g/L) was consumed completely with the rate of 1.80 g/L/h. Two-stage fermentation (combination of micro-aerobic and anaerobic condition) exhibited higher ethanol production (24.5 g/L) than using one-stage fermentation (either micro-aerobic or anaerobic condition. The two- stage configuration, exhibited slightly higher crude glycerol consumption rate (1.81 g/L/h), as well as ethanol yield (0.56 g/g) than the one-stage configuration. Therefore, two-stage process was selected for ethanol production from E. aerogenes TISTR1468 in scale-up studies.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1078939Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1673
 J. M. Marchetti, V. U. Miguel, and, A. F. Errazu, "Possible methods for biodiesel production,” Renew. Sust. Energy. Rev., vol. 11, pp. 1300-1311, 2007.
 M. A. Dasari, P. P. Kiatsimkul, W. R. Sutterlin, and G. J. Suppes, "Low-pressure hydrogenolysis of glycerol to propylene glycol,” Appl. Catalysis., vol. 281, no. 1-2, pp. 225-231, 2005.
 J. C. Thompson, and B. B. He, "Characterization of the crude glycerol from biodisel production from multiple feedstocks,” Appl. Eng. Agri., vol. 22, pp. 261-265, 2006.
 Z. M. Zheng, Q. L. Hu, J. Hao, F. Xu, N. N. Guo, Y. Sun, and D. H. Liu, "Statistical optimization of culture condition for 1,3-propanediol by Klebsiella pneumoniae AC15 via central composite design,” Boresour. Technol. vol. 99, pp. 1052-1056, 2008.
 S. Sattayasamitsathit, P. Prasertsan, and P. Methacanon, "Statistical optimization for simultaneous production of 1,3-propanediol and 2,3-butanediol using crude glycerol by newly bacterial isolate," Process Biochem vol. 46, pp. 608-614, 2011.
 N. Pachauri, and B. He, "Value-added utilization of crude glycerol from biodiesel production,” A Survey of Current Research Activities. An ASABE Meeting Presentation, Oregon, 2006.
 T. Ito, Y. Nakashimada, K. Senba, T. Matsui, and N. Nishio, "Hydrogen and ethanol production form glycerol-containing wastes discharged biodiesel manufacturing process,” J. Biosci. Bioeng., vol. 100, no. 3, pp. 260-265, 2005.
 M. F. Temudo, R. Poldermans, R. Kleerebezem, and M. C. M. Loosdrecht, "Glycerol fermentation by (open) mixed cultures: a chemostat study,” Biotechnol. Bioeng., vol. 100, pp. 1088-1098, 2008.
 A. Gupta, A. Murarka, P. Campbell, and R. Gonzalez, "Anaerobic fermentation of glycerol in Paenibacillus macerans: metabolic pathways and environmental determinants,” Appl. Environ. Microbiol., vol. 75, pp. 5871-5883, 2009.
 R. B. Oh, J. W. Seo, S. Y. Heo, W. K. Hong, L. H. Luo, M. H. Joe, D. H. Park, and C. H. Kim, "Efficient production of ethanol from crude glycerol by a Klebsiella pneumoniae mutant strain," Bioresour. Technol., vol. 102, pp. 3918-3922, 2001.
 W. J. Choi, M. R. Hartono, W. H. Chan, and S. S. Yeo, "Ethanol production from biodiesel-derived crude glycerol by newly isolated Kluyvera cryocrescens," Appl. Microbiol. Biotechnol., vol. 89, pp. 1255-1264, 2011.
 C. Tongurai, S. Klinpikul, C. Bunyakan, and P. Kiatsimkul, "Biodiesel production from palm oil,” Songkla. J. Sci. Technol., vol. 23, pp. 831-841, 2001.
 F. Barbirato, S. Astruc, P. Soucaille., C. Camarasa, J. M. Salmon, and A. Bories, "Anaerobic pathways of glycerol dissimilation by Enterobacter agglomerans CNCM1210: limitations and regulations,” Microbiol., vol. 43, pp. 2423-2432, 1997.
 E. V. Handel, "Suggested modification of micro determination of triglycerides,” Clinical Chem., vol. 7, no. 3, pp. 249-251, 1961.
 X. Chen, D. J. Zhang, V. T. Qi, S. J. Gao, Z. L. Xiu, and P. Xu, "Microbial fed-batch production of 1,3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions,” Appl. Microbiol. Biotechnol., vol. 63, pp. 143-146, 2003.
 S. S. Yazdari, and R. Gonzalez, "Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry,” Curr. Opin. Biotechnol., vol. 18, pp. 213-219, 2007.
 G. Yang, J. Tian, and J. Li, "Fermentation of 1,3-propanediol by a lactate deficient mutant of Klebsiella oxytoca under microaerobic conditions," Appl. Micronbiol., vol. 73, pp. 1017-1024, 2007.