%0 Journal Article %A Kanokrat Saisaard and Irini Angelidaki and Poonsuk Prasertsan %D 2011 %J International Journal of Biotechnology and Bioengineering %B World Academy of Science, Engineering and Technology %I Open Science Index 53, 2011 %T Micro-aerobic, Anaerobic and Two-stage Condition for Ethanol Production by enterobacter aerogenes from Biodiesel-derived Crude Glycerol %U https://publications.waset.org/pdf/12300 %V 53 %X The microbial production of ethanol from biodiesel¬derived crude glycerol by Enterobacter aerogenes TISTR1468, under micro-aerobic and anaerobic conditions, was investigated. The experimental results showed that micro-aerobic conditions were more favorable for cellular growth (4.0 g/L DCW), ethanol production (20.7 g/L) as well as the ethanol yield (0.47 g/g glycerol) than anaerobic conditions (1.2 g/L DCW, 6.3 g/L ethanol and 0.72 g/g glycerol, respectively). Crude glycerol (100 g/L) was consumed completely with the rate of 1.80 g/L/h. Two-stage fermentation (combination of micro-aerobic and anaerobic condition) exhibited higher ethanol production (24.5 g/L) than using one-stage fermentation (either micro-aerobic or anaerobic condition. The two- stage configuration, exhibited slightly higher crude glycerol consumption rate (1.81 g/L/h), as well as ethanol yield (0.56 g/g) than the one-stage configuration. Therefore, two-stage process was selected for ethanol production from E. aerogenes TISTR1468 in scale-up studies. %P 311 - 314