Inhibition Effect of Brazilin to Human Bladder Cancer Cell Line T24

Authors: Liansheng Ren, Xihua Yang, Guoping Wang, Hong Zhang, Lili Zhao, Zhenguo Mi

Abstract:

The inhibition effect of brazilin to human bladder tumor cell line T24 in vitro and in vivo was studied. The results of the in vitro experiments showed that brazilin has strong inhibition activity on the target cells. The inhibition ratio of 100 μg/mL brazilin and 100 μg/mL mitomycin to the target cells was 90.90 % and 63.24 % respectively, which showed that brazilin has higher inhibition activity than mitomycin under the same concentration. Brazilin could induce cell apoptosis in T24 cells. Significant antitumor activity of brazilin was also showed in the animals experiments. The life extention rate of 200 mg/mL, 300 mg/kg, and 400 mg/kg brazilin intraperitoneally injected into Balb/c-nu-nu nude mice that with human bladder cancer were 51.50 %, 56.90 %, and 58.42 %（P<0.05）. Our study showed that brazilin has significant inhibitory effect on human bladder tumor cell.

Keywords: bladder cancer, brazilin, inhibition, T24 cell line

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1077615

References:

[1] XM Zhang, GP Wang, LS Ren. Study of Caesalpinia Sppan aqueous extract-s apoptosis-inducing effects on human ovarian cancer cell SKOV3. Cancer Research and Clinic 2010; 22: 388-390.
[2] XM Zhang, GP Wang, LS Ren. Caesalpinia Sppan aqueous extract-s growth inhibiting and apoptosis-inducing effects on human ovarian cancer cell. Cancer Research and Clinic 2010; 22: 243-246.
[3] LS Ren, H Zhang, XH Bai, XB Han, ZG Mi. Hematoxylin-s cytocidal and apoptosis effects on human urinary cancer cell-T24. Cancer Research and Clinic 2008; 20: 799-801.
[4] AL Liu , SH Shu , HL Qin , SM Lee , YT Wang , GH Du. In vitro anti-influenza viral activities of constituents from Caesalpinia sappan. Planta Med 2009; 75: 337-339.
[5] CM Hu , YH Liu , KP Cheah , JS Li , CS Lam , WY Yu , CS Choy . Heme oxygenase-1 mediates the inhibitory actions of brazilin in RAW264.7 macrophages stimulated with lipopolysaccharide. J Ethnopharmacol 2009; 121: 79-85.
[6] IK Bae , HY Min , AR Han , EK Seo , SK Lee . Suppression of lipopolysaccharide-induced expression of inducible nitric oxide synthase by brazilin in RAW 264.7 macrophage cells. Eur J Pharmacol 2005; 513 : 237-242.
[7] CK Moon , KS Park , SG Kim , HS Won , JH Chung . Brazilin protects cultured rat hepatocytes from BrCCl3-induced toxicity. Drug Chem Toxicol 1992; 15: 81-91.
[8] LY Khil , SS Han , SG Kim , TS Chang , SD Jeon , DS So , CK Moon . Effects of brazilin on GLUT4 recruitment in isolated rat epididymal adipocytes. Biochem Pharmacol 1999; 58: 1705-1712.
[9] HS Won , J Lee , LY Khil, SH Chae , MY Ahn , BH Lee , JH Chung , YC Kim , CK Moon . Mechanism of action of Brazilin on gluconeogenesis in isolated rat hepatocytes. Planta Med 2004; 70: 740-744.
[10] EJ You , LY Khil , WJ Kwak , HS Won , SH Chae , BH Lee , CK Moon . Effects of brazilin on the production of fructose-2,6- bisphosphate in rat hepatocytes. J Ethnopharmacol 2005; 102(1): 53-57.
[11] HX Xu , SF Lee . The antibacterial principle of Caesalpina sappan. Phytother Res 2004; 18: 647-651.
[12] CM Hu , JJ Kang , CC Lee , CH Li, JW Liao , YW Cheng . Induction of vasorelaxation through activation of nitric oxide synthase in endothelial cells by brazilin. Eur J Pharmacol 2003; 468: 37-45.
[13] YW Xie , DS Ming , HX Xu , H Dong , PP But . Vasorelaxing effects of Caesalpinia sappan involvement of endogenous nitric oxide. Life Sci 2000; 67: 1913-1918.
[14] GS Hwang , JY Kim , TS Chang , SD Jeon , DS So , CK Moon . Effects of Brazilin on the phospholipase A2 activity and changes of intracellular free calcium concentration in rat platelets. Arch Pharm Res 1998; 21: 774-778.
[15] KM Yang , SD Jeon , DS So , Moon CK. Brazilin augments cellular immunity in multiple low dose streptozotocin (MLD-STZ) induced type I diabetic mice. Arch Pharm Res 2000 ; 23: 626-632.
[16] MS Mok , SD Jeon , KM Yang , DS So , CK Moon . Effects of Brazilin on induction of immunological tolerance by sheep red blood cells in C57BL/6 female mice. Arch Pharm Res 1998; 21: 769-773.
[17] SY Choi , KM Yang , SD Jeon, JH Kim , LY Khil , TS Chang , Moon CK. Brazilin modulates immune function mainly by augmenting T cell activity in halothane administered mice. Planta Med 1997; 63: 405-408.
[18] W Mar , HT Lee , KH Je , HY Choi , EK Seo . A DNA strand-nicking principle of a higher plant, Caesalpinia sappan. Arch Pharm Res 2003; 26: 147-150