Vitamin Content of Swordfish (Xhiphias gladius) Affected by Salting and Frying
The swordfish (Xiphias gladius) is a large oceanic fish of high commercial value, which is widely distributed in waters of the world’s oceans. They are considered to be an important source of high quality proteins, vitamins and essential fatty acids, although only half of the population follows the recommendation of nutritionists to consume fish at least twice a week. Swordfish is consumed worldwide because of its low fat content and high protein content. It is generally sold as fresh, frozen, and as pieces or slices. The aim of this study was to evaluate the effect of salting and frying on the composition of the water-soluble vitamins (B2, B3, B9 and B12) and fat-soluble vitamins (A, D, and E) of swordfish. Three loins of swordfish from Pacific Ocean were analyzed. All the fishes had a weight between 50 and 70 kg and were transported to the laboratory frozen (-18 ºC). Before the processing, they were defrosted at 4 ºC. Each loin was sliced and salted in brine. After cleaning the slices, they were divided into portions (10×2 cm) and fried in olive oil. The identification and quantification of vitamins were carried out by high-performance liquid chromatography (HPLC), using methanol and 0.010% trifluoroacetic acid as mobile phases at a flow-rate of 0.7 mL min-1. The UV-Vis detector was used for the detection of the water- and fat-soluble vitamins (A and D), as well as the fluorescence detector for the detection of the vitamin E. During salting, water and fat-soluble vitamin contents remained constant, observing an evident decrease in the values of vitamin B2. The diffusion of salt into the interior of the pieces and the loss of constitution water that occur during this stage would be related to this significant decrease. In general, after frying water-soluble and fat-soluble vitamins showed a great thermolability with high percentages of retention with values among 50–100%. Vitamin B3 is the one that exhibited higher percentages of retention with values close to 100%. However, vitamin B9 presented the highest losses with a percentage of retention of less than 20%.
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 N. Cobas, S. Martínez, and I. Franco, Pez Espada: Pesca, Composición Química y Valor Nutricional. Vigo, Spain: Universidade de Vigo. Servizo de Publicacións; 2016.
 N. Abid and M. Idrissi, “Descripción del pez espada (SWO),” in Manual de ICCAT. Comisión Internacional para la Conservación del Atún Atlántico, Publicaciones ICCAT, 2006, ch. 2.1.9.
 MERCASA, Productos de la pesca y la acuicultura. (online). 2016. (Accessed 24/01/2019). Available from: http://www.mercasa-ediciones.es/alimentacion_2016/pdfs/Sectores/Productos_pesca_y_acuicultura_2016.pdf.
 S.P. Lall and M.P. Parazo, “Vitaminas del pescado y del marisco,” in El Pescado y los Productos Derivados de la Pesca, A. Ruiter, and B.P. Sanz, Ed. Acribia, Zaragoza, 1999, ch. 6.
 E. Lešková, et al. “Vitamin losses: Retention during heat treatment and continual changes expressed by mathematical models,” J. Food Comp. Anal., vol. 19, no. 4, pp. 252–276, Sept. 2013.
 S. Dergal, “Vitaminas y nutrientes inorgánicos,” in Química de los Alimentos, 4rd ed. S. Dergal, Ed. México: Pearson Educación, 2006, pp. 363–400.
 B. Klejdus, et al. “Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection,” Anal. Chim. Acta, vol. 520, no. 1, pp. 57–67, Aug. 2004.
 T. Saldanha, A.C.H.S. Frankland, M.E. Nogueira, and N. Bragagnolo, “HPLC Separation and determination of 12 cholesterol oxidation products in fish: Comparative Study of RI, UV, and APCI-MS Detectors,” J. Agric. Food Chem., vol. 54, no. 12, pp. 4107–4113, Jun. 2016.
 J.C. Miller, and J.N. Miller, Statistic of Analytical Chemistry. Englewood Cliffs, N.J.: Prentice-Hall, 1999.
 O. Moreiras, A. Carbajal, L. Cabrera, and C. Cuadrado, “Tablas de Composición de Alimentos”. Madrid: Pirámide, 2013.
 B. Ersoy, and A. Özeren, A, “The effect of cooking methods on mineral and vitamin contents of African catfish,” Food Chem., vol. 115, no. 2, pp. 419–422, July 2009.
 USDA, Nutrient Database for Standard Reference, Laboratory, Agricultural Research Service. (online). 2014. (Accessed 10/01/2019). Available from: http://www.nal.usda.gov/fnic/foodcomp/search/.
 M.G. Dias, M.V. Sánchez, H. Bártolo, and L. Oliveira, “Vitamin content of fish and fish products consumed in Portugal”, EJEAFChe., vol. 2, no. 4, pp. 510–513, Sept. 2003.
 A. Badiani, et al., “True retention of nutrients upon household cooking of farmed portion-size European sea bass (Dicentrarchus labrax L.),” LWT-Food Sci. Technol., vol. 50, no.1, pp. 72–77, Jan. 2013.
 D.A. Dobreva, et al., “Black sea fish and shelfish as essential source of vitamin B12,” Int. J. Sci. Rep., vol. 4, no. 8, pp. 199–203, Aug. 2018.
 F. Hoffmann-La Roche, Vitamin Compendium: the properties of the vitamins and their importance in human and animal nutrition. Basle, Switzerland: Roche, 1976.
 A.S. Al-Khalifa, and A.A. Dawood, “Effects of cooking methods on thiamin and riboflavin contents of chicken meat,” Food Chem., vol. 48, no. 1, 1993, 69–74.
 E. Mašková, J. Rysová, V. Fiedlerová, and M. Holasová, “Vitamin and mineral retention in meat in various cooking methods,” Czech J. Food Sci., vol. 12, no. 5, pp. 407–416, 1994.
 A. Lassen, M. Kall, K. Hansen, and L. Ovesen, “A comparison of the retention of vitamins B1, B2 and B6 and cooking yield in pork loin with conventional and enhanced meal-service systems,” Eur. Food Res. Technol., vol. 215, no. 3, pp. 194–199, Sept. 2002.
 G.F.M. Ball, Water-soluble Vitamin Assay in Human Nutrition. London, NY: Chapman & Hall, 1994.
 R.R. Eitenmiller, and W.O. Laden, Vitamin Analysis for the Health and Food Science. Boca Raton, Florida: CRC Press, 1999.
 F. Watanabe, “Vitamin B12 Sources and Bioavailability,” Exp. Biol. Med., vol. 232, no. 10, pp. 1266–1274, Nov. 2007.
 E.W. Murphy, P.E. Criner, and B.C. Gray, “Comparison of methods for determining retentions of nutrients in cooked foods,” J. Agric. Food Chem., vol. 23, no. 6, pp. 1153–1157, Nov. 1975.
 A. Polat, et al., “Tocopherol content of commercial fish species as affected by microwave cooking,” J. Food Biochem., vol. 37, pp. 381–387, Sept. 2011.
 W.C. Byrdwell, et al., “Vitamin D levels in fish and shellfish determined by liquid chromatography with ultraviolet detection and mass spectrometry,” J. Food Comp. Anal., vol. 30, no. 2, pp. 109–119, Jun. 2013.
 D. Kilcast, “Effect of irradiation on vitamins,” Food Chem., vol. 49, no. 2, pp. 157–164, 1994.
 D. Lund, “Effects of heat processing on nutrients,” in Nutritional Evaluation of Food Processing, E. Karmas, and R.S. Harries, Ed. Dordrecht: Springer, 1988, pp. 319–354.
 B. Holland, et al., “Fish and Fish Products” in The Composition of Foods, 5th ed. McCance and Widdowson’s, Cambridge: Royal Society of Chemestry, 1993.
 A.H. Simonne, and R.R. Eitenmiller, “Retention of vitamin E and added retinyl palmitate in selected vegetable oils during deep-fat frying and fried breaded products,” J. Agric. Food Chem., vol. 46, no. 12, pp. 5273–5277, 1998.
 M.A. Murcia, M. Martínez-Tomé, I. Del Cerro, F. Sotillo, and A. Ramírez, “Proximate composition and vitamin E levels in egg yolk: losses by cooking in a microwave oven,” J. Sci. Food Agric., vol. 79, no. 12, pp. 1550–1556, Aug. 1999.