Search results for: Retama monosperma

Authors: N. Selami, M. Kaid Harche

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A pink-pigmented, aerobic, facultatively methylotrophic bacterium, was isolated from Retama monosperma root nodules and identified as a member of the genus Methylobacterium. Inoculation of R. monosperma plants by a pure culture of isolate strain under a hydroponic condition, resulted, 10 dpi, the puffiness at lateral roots. The observation in detail the anatomy and ultra-structure of infection sites by light and electron microscopy show that the bacteria induce stimulation of the division of cortical cells and digestion of epidermis cells then, Methylobacterium was observed in the inter and intracellular spaces of the outer cortex root. These preliminary results allow us to suggest the establishment of an epi-endosymbiotic interaction between Methylobacterium sp and R. monosperma.

Keywords: endophytic colonization, Methylobacterium, microscopy, nodule, pink pigmented, Retama monosperma

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Authors: A. V. Shrirao, N. I. Kochar, A. V. Chandewar

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The flower extract of Butea monosperma herb has been used traditionally in India for medicinal purposes. The plant has been reported to treat hyperglycemia and associated hyperlipidemia. Hyperlipidemia and oxidative stress are known to accelerate coronary artery disease and progression of atherosclerotic lesions. The present work was undertaken to investigate the possible antihyperlipidemic and antioxidative effect of Butea monosperma flowers on hyperlipidemic rats. Hyperlipidemia was induced in rats by a single intraperitonial (i.p.) injection of Triton WR 1339 (400 mg/kg) and it showed sustained elevated levels of serum cholesterol and triglyceride. Ethanolic extract of Butea monosperma flowers (Et-BM) (250 and 500 mg/kg/day) was administered to normal and hyperlipidemic rats for 14 days. Serum and liver tissue were analyzed at three different time intervals for lipid profile and antioxidants enzymes and the activity were compared to the cholesterol-lowering drug, Atorvastatin (10 mg/kg). Parameters were altered during hyperlipidemia and reverted back to near normal values after Et-BM treatment or standard drug Atorvastatin. Lipid peroxidation decreased whereas the activities of superoxide dismutase, glutathione peroxidase and catalase increased in Et-BM treated rats. Pronounced changes were observed at 500 mg/kg of Et-BM for 2 weeks and it was comparable to the standard drug Atorvastatin. The current study provides strong evidence that Et-BM has a remarkable beneficial effect in treating hyperlipidemia and ROS without any side effects at the dosage and duration studied.

Keywords: antioxidant, butea monopserma, hyperlipidemia, triton WR 1339

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Authors: Archna Mall, Neelam Agrawal, Hari O. Saxena, Bhavana Sharma

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Butea monosperma occurs widely throughout central Indian states. Eco-friendly natural dyes were isolated in aqueous medium from leaves, bark and flowers of this plant. These dyes were used for dyeing on cotton fabric using various chemical (potassium aluminium sulphate, potassium dichromate, ferrous sulphate, stannous chloride & tannic acid) and natural mordants (rinds of Terminallia bellerica & Terminalia chebula fruits and shells of Prunus dulcis & Juglans regia nuts). Dyeing was carried out using the pre-mordanting technique. Large range of beautiful shades in terms of hue and darkness were recorded because of varying mordant concentrations and combinations. More importantly dyed fabrics registered varying the degree of colour fastness properties to washing (1-3, colour change and 4-5, colour staining), light (2-4), rubbing (4-5, dry and 3-5, wet) and perspiration (1-4, colour change and 4-5, colour staining). Thus, along with flowers which are traditionally known for natural dyes, the leaves and bark may also find their place in textile industries.

Keywords: Butea monosperma, cotton, mordants, natural dyes

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Authors: Syed Farooq Adil, Merajuddinkhan, Mujeeb Khan, Hamad Z. Alkhathlan

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Phytochemicals from plant extracts belong to an important source of natural products which have demonstrated excellent cytotoxic activities. However, plants of different origins exhibit diverse chemical compositions and bioactivities. Therefore, the discovery of plants based new anticancer agents from different parts of the world is always challenging. In this study, methanolic extracts of different parts of 11 plants from Saudi Arabia have been tested in vitro for their anticancer potential on human liver cancer cell line (HepG2). Particularly, for this study, plants from Asteraceae, Resedaceae, and Polygonaceae families were chosen on the basis of locally available ethnobotanical data and their medicinal properties. Among 12 tested extract samples, three samples obtained from Artemisia monosperma stem, Ochradenus baccatus aerial parts, and Pulicaria glutinosa stem have demonstrated interesting cytotoxic activities with a cell viability of 29.3%, 28.4% and 24.2%, respectively. Whereas, four plant extracts including Calendula arvensis aerial parts, Scorzonera musilii whole plant, A. monosperma leaves show moderate anticancer properties bearing a cell viability ranging from 11.9 to 16.7%. The remaining extracts have shown poor cytotoxic activities. Subsequently, GC-MS analysis of methanolic extracts of the four most active plants extracts such as C. comosum, O. baccatus, P. glutinosa and A. monosperma detected the presence of 41 phytomolecules. Among which 3-(4-hydroxyphenyl) propionitrile (1), 8,11-octadecadiynoic acid methyl ester (2), 6,7-dimethoxycoumarin (3), and 1-(2-hydroxyphenyl) ethenone (4) were found to be the lead compounds of C. comosum, O. baccatus P. glutinosa and A. monosperma, respectively.

Keywords: medicinal plants, asteraceae, polygonaceae, hepg2

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Authors: Reenu Yadav, S. K. Yadav

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Herbal therapy has been used for daily oral health care to prevent, treat or cure oral conditions from halitosis to periodontal diseases. The importance of mouth and teeth cleanliness has been recognized from the earliest days of civilization to the 21st century. In the present study, leaves and seeds of Cordia obliqua and barks and twigs of Beautea monosperma, which is used traditionally for oral diseases was evaluated for its antimicrobial activity. The antimicrobial activity tests indicated that the methanolic extract exhibited stronger activities against the commonly encountered oral bacterial and fungal pathogens. The mouthwash formulation prepared and it is compared with marketed formulation HiOra. The results indicated that the herbal mouthwash could inhibit the growth of oral pathogens and may prevent plaque and other periodontal diseases caused by dental pathogens.

Keywords: herbal mouthwash, bio medicine, life sciences, herbal extracts

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Authors: Gelacio Juárez-Luna, Gustavo Ayala, Jaime Retama-Velasco

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This paper shows the advantages of the material failure process simulation by improve finite elements with embedded discontinuities, using a new definition of traction vector, dependent on the discontinuity length and the angle. Particularly, two families of this kind of elements are compared: kinematically optimal symmetric and statically and kinematically optimal non-symmetric. The constitutive model to describe the behavior of the material in the symmetric formulation is a traction-displacement jump relationship equipped with softening after reaching the failure surface. To show the validity of this symmetric formulation, representative numerical examples illustrating the performance of the proposed formulation are presented. It is shown that the non-symmetric family may over or underestimate the energy required to create a discontinuity, as this effect is related with the total length of the discontinuity, fact that is not noticed when the discontinuity path is a straight line.

Keywords: variational formulation, strong discontinuity, embedded discontinuities, strain localization

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Authors: Reenu Yadav, Vidhi Guha, Udit N. Soni, Jay Ram Patel

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Chewing gums are mobile novel drug delivery systems, with a potential for administering drugs either for local action or for systemic absorption via the buccal route. An antimicrobial chewing gum delivery system of the methanolic extracts of Beatea monosperma (barks and twigs), Cordia obliqua (leaves and seeds) and Cuminun cyminum (seeds) against periodontal diseases caused by some oral pathogens, was designed and characterized on various parameters.The results of the study support the traditional application of the plants and suggest, plant extracts possess compounds with antimicrobial properties that can be used as potential antimicrobial agents and gums can be a good carrier of herbal extracts. Developed formulation will cure/protect from various periodontal diseases. Further development and evaluations chewing gums including the isolated compounds on the commercial scale and their clinical and toxicological studies are the future challenges.

Keywords: periodontal diseases, herbal chewing gum, herbal extracts, novel drug delivery systems

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Authors: Jitin Rahul, Manish Kumar Jain

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Dust pollution refers to the various locations, activities, or factors which are responsible for the releasing of pollutants into the atmosphere. The sources of dust can be classified into two major categories anthropogenic sources (man-made sources) and natural sources. Dust kicked up by heavy vehicles (Bus, Truck, Loaders, Tankers, car etc.) travelling on highways may make up approximately 33-40% of air pollution. Plants naturally cleanse the atmosphere by absorbing gases and particulate matter plants (Leaves). Plants are very good pollution indicator and also very good for dust capturing (Dust controlling). Many types tree species like Azadirachta indica A. juss, Butea monosperma (Lam.) Kuntz., Ficus bengalensis (Linn)., Pterocarpus marspium (Roxb.), Terminalia arjuna (Roxb, exDC.), Dalbergia sissoo roxb., and Ficus religiosa (Linn.) generally occur in roadside. These selected tree spiciness can control the dust pollution or dust capturing. It is well known that plants absorb particulate pollutants and help in dust controlling. Some tree species like (Ficus bengalensis, Ficus religiosa and Azadirachta indica) are very effective and natural means for controlling air pollution.

Keywords: dust, pollution, road, tree species

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Authors: Ramandeep Kaur M. Malhi, Prashant K. Srivastava, G.Sandhya Kiran

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Quantitative estimates of foliar biochemicals, namely chlorophyll content (CC), serve as key information for the assessment of plant productivity, stress, and the availability of nutrients. This also plays a critical role in predicting the dynamic response of any vegetation to altering climate conditions. The advent of hyperspectral data with an enhanced number of available wavelengths has increased the possibility of acquiring improved information on CC. Retrieval of CC is extensively carried through well known spectral indices derived from hyperspectral data. In the present study, an attempt is made to develop hyperspectral indices by identifying optimum bands for CC estimation in Butea monosperma (Lam.) Taub growing in forests of Shoolpaneshwar Wildlife Sanctuary, Narmada district, Gujarat State, India. 196 narrow bands of EO-1 Hyperion images were screened, and the best optimum wavelength from blue, green, red, and near infrared (NIR) regions were identified based on the coefficient of determination (R²) between band reflectance and laboratory estimated CC. The identified optimum wavelengths were then employed for developing 12 hyperspectral indices. These spectral index values and CC values were then correlated to investigate the relation between laboratory measured CC and spectral indices. Band 15 of blue range and Band 22 of green range, Band 40 of the red region, and Band 79 of NIR region were found to be optimum bands for estimating CC. The optimum band based combinations on hyperspectral data proved to be the most effective indices for quantifying Butea CC with NDVI and TVI identified as the best (R² > 0.7, p < 0.01). The study demonstrated the significance of band characterization in the development of the best hyperspectral indices for the chlorophyll estimation, which can aid in monitoring the vitality of forests.

Keywords: band, characterization, chlorophyll, hyperspectral, indices

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Authors: Raees Khan, Mir A. Khan, Sheikh Z. Ul Abidin, Abdul S. Mumtaz

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In the present study, phytogeography and conservation status of gymnosperms of Pakistan were investigated. 44 gymnosperms species of 18 genera and 9 families were collected from 66 districts of the country. Among the 44 species, 20 species were native (wild) and 24 species were exotic (cultivated). Ephedra sarocarpa of Ephedraceae was not collected in this study from its distribution area and most probably it may be Nationally Extinct now from this area. Previously in Gymnosperms Flora of Pakistan 34 species was reported. 12 new gymnosperms species were recorded for the first time. Pinus wallichiana (40 districts), Cedrus deodara (39 districts) Pinus roxburghii (36 districts), Picea smithiana (36 districts) and Abies pindrow (34 districts) have the maximum ecological amplitude. Juniperus communis (17districts) and Juniperus excelsa (14 districts) were the widely distributed among the junipers. Ephedra foliata (23 districts), Ephedra gerardiana (20 districts) and Ephedra intermedia (19 districts) has the widest distribution range. Taxus fuana was also wider distribution range and recorded in 19 districts but its population was not very stable. These species was recorded to support local flora and fuana, especially endemics. PCORD version 5 clustered all gymnosperms species into 4 communities and all localities into 5 groups through cluster analyses. The Two Way Cluster Analyses of 66 districts (localities) resulted 4 various plant communities. The Gymnosperms in Pakistan are distributed in 3 floristic regions i.e. Western plains of the country, Northern and Western mountainous regions and Western Himalayas. The assessment of the National conservation status of these species, 10 species were found to be threatened, 6 species were endangered, 4 species were critically endangered and 1 species have become extinct (Ephedra sarcocarpa). The population of some species i.e. Taxus fuana, Ephedra gerardiana, Ephedra monosperma, Picea smithiana and Abies spectabilis is decreasing at an alarming rate.

Keywords: conservation status, gymnosperms, phytogeography, Pakistan

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Authors: Reenu Yadav, Abhay Asthana, S. K. Yadav

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Purpose: The aim of the present work was to develop an oral gel for brushing with an antimicrobial activity which will cure/protect from various periodontal diseases such as periodontitis, gingivitis, and pyorrhea. Methods: Plant materials procured from local suppliers, extracted and standardized. Screening of antimicrobial activity was carried out with the help of disk diffusion method. The gel was formulated by dried extracts of Beautea monosperma and Cordia obliquus. Gels were evaluated on various parameters and standardization of the formulation was performed. The release of drugs was studied in pH 6.8 using a mastication device.Total phenolic and flavonoid contents were estimated by folin-Ciocalteu and aluminium chloride method, and stability studies were performed (40°C and RH 75% ± 5% for 90 days) to assess the effect of temperature and humidity on the concentration of phenolic and flavonoid contents. The results of accelerated stability conditions were compared with that of samples kept at controlled conditions (RT). The control samples were kept at room temperature (25°C, 35% RH for 180 days). Results: Results are encouraging; extracts possess significant antimicrobial activity at very low concentration (15µg/disc, 20µg/disc and 15 µg/ disc) on oral pathogenic bacteria. The formulation has optimal characteristics, as well as has a pleasant appearance, fragrance, texture, and taste, is highly acceptable by the volunteers. The diffusion coefficient values ranged from 0.6655 to 0.9164. Since the R values of korsmayer papas were close to 1, Drug release from formulation follows matrix diffusion kinetics. Hence, diffusion was the mechanism of the drug release. Formulation follows non-Fickian transport mechanism. Most Formulations released 50 % of their contents within 25-30 minutes. Results obtained from the accelerated stability studies are indicative of a slight reduction in flavonoids and phenolic contents with time on long time storage. When measured degradation under ambient conditions, degradation was significantly lower than in accelerated stability study. Conclusion: Plant extracts possess compounds with antimicrobial properties can be used as. Developed formulation will cure/protect from various periodontal diseases. Further development and evaluations oral gel including the isolated compounds on the commercial scale and their clinical and toxicological studies are the future challenges.

Keywords: herbal gel, dental care, ambient conditions, commercial scale

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Authors: Munruchi Kaur

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Agarics are the fruiting bodies of the fungi falling under Phylum Basidiomycota of class Agaricomycetes. North Western parts of India which comprises of mighty Himalayas decorated with snow cap mountains, forested areas, grassland and the Gangetic plains with the altitude varying between 196m to 3600m have a huge potential of naturally growing wild agarics. These mushrooms lavishly grow in wet humid weather conditions that prevail in these parts of India during the monsoon which hits in the early June and continue up to mid-October. In this area, a diverse form of mixed vegetation is available which is represented by coniferous and angiospermic trees, shrubs, herbs, epiphytes, parasites, climbers etc. The vegetation, topography and climate of this area is quite favorable for the growth of agarics. Cedrus deodara, Pinus longifolia, P. roxburghii, P. wallichiana, Abies pindrow, A. spectabilis, Picea smithiana, Taxus sp., Rhododendron sp. and Quercus sp. occur in pure formations or as scattered patches or as mixed forests, whereas the Gangetic plains are dominated by the angiospermic trees and shrubs, they commonly occur along roadsides or in conserved areas or are the avenues plantations, common amongst these are Shorea robusta, Dalbergia sissoo, Melia azadirachta, Acacia sp., Ficus benghalensis, Eucalyptus sp. and Butea monosperma. These agarics can be categorized on the basis of the habitat in which they grow they are usually foliocolous, lignicolous, humicolous, coprophilous or termitophilous. A number of fungal forays were undertaken to different parts of North West India from time to time during the monsoon season with an aim to decipher the agarics diversity of this part of India. Along with collecting the various agarics from diverse habitat, the ethnomycological data was also collected along with by interacting with the local inhabitants of those areas. Based upon the ethnomycological data collected over the years, cataloging of the edible and inedible agarics has been done and cultures of such potential edible agarics were raised with an aim to domesticate these selected taxa. With an aim to reduce the local pressure on these natural resources, a low-cost technology was developed to make it available to the public for cultivation. As a result, 104 taxa were found edible such as Amanita hemibapha var. ochracea, A. chepangiana, A. banningiana, A. vaginata, Agrocybe parasitica, Author: Professor & Dean Faculty of Life Sciences Punjabi University, Patiala. Punjab, India [email protected] Agaricus bisporus, A. andrewii, A. campestris var. campestris, A. silvicola, A. subrutilescens, A. bernardii, A. abruptibulbus, A. fuscovelatus, A. brunnescens, A. augustus, A. silvaticus, A. arvensis, Volvariella bakeri, V. terastia, V. bombycina, V. diplasia, Psathyrella candolleana, Volvopluteus gloiocephalus, Russula cyanoxantha, R. atropurpurea, R. aurea, Clitocybe gibba,Lentinus transitus, L. kashmirinus, L. crinitus, L. ligrinus, Lactarius rubrilacteus, Pleurotus sapidus, Pluteus subcervinus, Macrocybe gigantea, etc. Cultures of various taxa viz. Pleurotus sajor-caju, Macrocybe gigantea, Pluteus petasatus and Lentinus tigrinus were raised and a proper protocol for the domestication of Pleurotus sajor-caju, Macrocybe gigantea, and Lentinus tigrinus has been developed using the locally available agro-wastes.

Keywords: Agaric, culture, domestication, edible

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