Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 31097
Prevalence and Fungicidal Activity of Endophytic Micromycetes of Plants in Kazakhstan

Authors: L. V. Ignatova, Y. V. Brazhnikova, T. D. Mukasheva, R. Zh. Berzhanova, A. A. Omirbekova


Endophytic microorganisms are presented in plants of different families growing in the foothills and piedmont plains of Trans-Ili Alatau. It was found that the maximum number of endophytic micromycetes is typical to the Fabaceae family. The number of microscopic fungi in the roots reached (145.9±5.9)×103 CFU/g of plant tissue; yeasts - (79.8±3.5)×102 CFU/g of plant tissue. Basically, endophytic microscopic fungi are typical for underground parts of plants. In contrast, yeasts more infected aboveground parts of plants. Small amount of micromycetes is typical to inflorescence and fruits. Antagonistic activity of selected micromycetes against Fusarium graminearum, Cladosporium sp., Phytophtora infestans and Botrytis cinerea phytopathogens was detected. Strains with a broad, narrow and limited range of action were identified. For further investigations Rh2 and T7 strains were selected, they are characterized by a broad spectrum of fungicidal activity and they formed the large inhibition zones against phytopathogens. Active antagonists are attributed to the Rhodotorula mucilaginosa and Beauveria bassiana species.

Keywords: Prevalence, endophytic micromycetes, fungicidal activity

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2277


[1] B. Schulz, C. Boyle, Th. Sieber, Microbial Root Endophytes. Berlin:Springer-Verlag, 2006.
[2] D. Zhang, D. Spadaro, A. Garibalde, "Efficacy of the antagonist Aureobasidium pullulans PL5 against postharvest pathogens of peach, apple and plum and its modes of action,” Biological Control, vol. 54, no. 3, pp. 172-180, Sept.2010.
[3] P.A. Backman, R.A. Sikora, "Endophytes: An emerging tool for biological control,” Biological Control, vol.46, no. 1, pp. 1-3, July 2008.
[4] Ch.W. Bacon, J. White, Microbial Endophytes. CRC Press, 2000.
[5] M.A. Vinton, E.S. Kathol, K.P. Vogel, A.A. Hopkins, "Endophytic fungi in Canada wild rye in natural grasslands,” J. Range Manage, vol.54, no. 4, pp. 390-395, July 2001.
[6] J. Han, D. Xia, L. Li, Sun L., K. Yang, L. Zhang, "Diversity of cultirable bacteria isolated from root domains of Moso Bamboo (Phyllostachys edulis),” Microb.Ecology, vol.58, no.2, pp. 363-373, Aug. 2009.
[7] A.I. Netrusov, M.A. Egorova, Microbiology Workshop Handbook. Moscow: Academy, 2005.
[8] S.K. Gond, A.Mishra, V.K. Sharma, S.K. Verma, J. Kumar, "Diversity and antimicrobial activity of endophytic fungi isolated from Nytanthes arbor-tristis, a well-known medicinal plant of India,” Mycoscience, vol.53, no.2, pp. 113-121, March 2012.
[9] S. Jiang, D. Qian, N. Yang, "Biodiversity and Antimicrobial Activity of Endophytic Fungi in Angelica sinensis,” Chinese Herbal Medicines, vol.5, no.4, pp. 264-271, Nov. 2013.
[10] G. Xin, D. Glawe, S.L. Doty, "Characterization of three endophytic, indole-3-acetic acid-producing yeasts occurring in Populus trees,” Mycological Research, vol.113, no.9, pp. 973-980, Sept. 2009.
[11] D.I. Tsitsigiannis, M. Dimakopoulou, P.P. Antoniou, E.C. Tjamos, ”Biological control strategies of mycotoxigenic fungi and associated mycotoxins in Mediterranean basin crops,” Phytopathologia Mediterranea, vol.51, no.1, pp. 158-174, Jan. 2012.
[12] T.G. Mirchink, Soil Mycology. Moscow: Moscow State University, 1988.
[13] V.I. Bilai, Soil mycromycetes. Kiev: Naukova dumka Publ., 1984.
[14] H. Horinouchi, A. Muslim, T. Suzuki, M. Hyakumachi, "Fusarium equiseti GF191 as an effective biocontrol agent against Fusarium crown and root rot of tomato in rock wool systems,” Crop Protection, vol.26, no.10, pp. 1514–1523, Oct.2007.
[15] T.T. Tesso, N. Ochanda, C.R. Little, L. Claflin, M.R. Tuinstra, "Analysis of host plant resistance to multiple Fusarium species associated with stalk rot disease in sorghum
[Sorghum bicolor (L.) Moench],” Field Crops Research, vol.118, no.2, pp. 177–182, Aug.2010.
[16] K.A. Selim, A.A El-Beih, T.M. AbdEl-Rahman, A.I. El-Diwany, "Biology of Endophytic Fungi,” Current Research in Environmental & Applied Mycology, vol.2, no.1, pp. 31-82, June 2012.
[17] N. Akhtyamova, R. Battarova, "Endophytic Yeast Rhodotorula rubra Strain TG-1: Antagonistic and Plant Protection Activities,” Biochemistry and Physiology, vol.2, no.1, Dec. 2012, open access.
[18] B.H. Ownley, M.R. Griffin, W.E. Klingeman, K.D. Gwinn, J.K. Moulton, R.M. Pereira, "Beauveria bassiana: Endophytic colonization and plant disease control,” Journal of Invertebrate Pathology, vol.98, no.3, pp. 267-270, July 2008.
[19] B.H. Ownley, K.D. Gwinn, F.E. Vega, "Endophytic fungal entomopathogens with activity against plant pathogens: ecology and evolution,” BioControl, vol.55, no.1, pp. 113-128, Feb. 2010.