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Influence of Culturing Conditions on Biomass Yield, Total Lipid, and Fatty Acid Composition of Some Filamentous Fungi

Authors: Alla V. Goncharova, Tatyana A. Karpenyuk, Yana S. Tsurkan, Rosa U. Beisembaeva, Togzhan D. Mukasheva, Ludmila V. Ignatova, Ramza Z. Berzhanova


In this work the effect of culturing conditions of filamentous fungi Penicillium raistrickii, Penicillium anatolicum, Fusarium sp. on biomass yield, the content of total lipids and fatty acids was studied. It has been established that in time the process of lipids accumulation correlated with biomass growth of cultures, reaching maximum values in stationary growth phase.

Biomass yield and accumulation of general lipids was increased by adding zinc to the culture medium. The more intensive accumulation of biomass and general lipids was observed at temperature 18°C. Lowering the temperature of culturing has changed the ratio of saturated: Unsaturated fatty acids in the direction of increasing the latter.

Keywords: Biomass, culturing conditions, fungi, fatty acids (FA), growth dynamics, lipids.

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[1] Y.R. Rakhmatullina, "Development of method for receiving of polyunsaturated fatty acids,” Abstract of candidate of technical sciences, Ufa, 189 p., 2007,
[2] P.E. Nazarov, G.I. Myagkova, N.V. Groza, "Polyunsaturated fatty acids as universal endogenic bioregulators,” Bulletin of MSUTCT, vol. 4, no.5, pp. 3-19, 2009.
[3] S.D. Dyal, and S.S. Narine, "Implication for the use of Mortierella fungi in the industrial production of essential fatty acids,” Food Res. Intern., vol. 38, no. 4, pp. 445–467, 2005.
[4] E.G. Deduhina, T.I. Chistyakova, and M.B. Vainshtein, "Biosynthesis of arachidonic acid by Micromycetes (review),”Applied biochemistry and microbiology, vol. 47, no. 2, pp. 125-134, 2011.
[5] J. Kumar, and R. Banerjee, "Optimization of lipid enriched biomass production from oleaginous fungus using response surface methodology,” Indian Journal of Experimental biology, vol. 51, pp. 979-983, 2013.
[6] R. Subramaniam, S. Dufreche, and M. Zappi, "Microbial lipids from renewable resources: production and characterization,” J Ind Microbiol Biotechnol., no. 37, pp. 1271–1287, 2010.
[7] K. Higashiyama, S. Fujikawa, E. Park, and S. Shimizu, "Production of Arachidonic Acid by Mortierella Fungi,” Biotechnol. Bioprocess Eng., vol. 7, pp. 252-262, 2002.
[8] J. Garbus, H. F. Deluca, M. E. Loomans, and F. M.Strong, "Rapid incorporation of phosphate into mitochondrial lipids,” J.Biol.Chem., vol. 238, pp. 59-63, 1963.
[9] W.W. Christie, Lipid analysis. Isolation, separation, identification and structural analysis of lipids. Bridgwater: The Oily Press, 2003.
[10] A. Kendrick, and C. Ratledge, "Lipid formation in ther oleaginous mould Entomophthora exitalis grown in continuous culture: effects of growth rate, temperature and dissolved oxygen tension on polyunsaturated fatty acids," Appl. Microbiol. Biotechnol., vol. 37, pp. 18-22, 1992.
[11] C. Xia, J. Zhang, W. Zhang, and B. Hu, "A new cultivation method for microbial oil production: cell pelletization and lipid accumulation by Mucor circinelloides,” Biotechnology for Biofuels, vol. 4, 2011.
[12] T. A. Pedersen, "Lipid formation in Cryptococcus terricolus. Nitrogen nutrition and lipid formation,” Acta Chem. Scand., vol. 15, pp. 651-662, 1961.
[13] E. G. Dedyukhina, and V. K Eroshin, "Essential metal ions in the control of microbial metabolism,” Process Biochem., vol. 26, no. 1, pp. 31–37, 1991.
[14] H. Kaboosi, and B. Behbahani, ”An overview on effective parameters in production of single cell oil by microorganisms especially the fungus of Mortierella isabellina,” Annals of Biological Research.,vol. 3, pp. 1650 – 1654, 2012.
[15] D. Warude, K. Joshi, and A. Harsulkar, "Polyunsaturated fatty acids: biotechnology,” Crit. Rev. Biotechnol., vol. 26, no. 2, pp. 83–93, 2006.