Levels of Some Antinutritional Factors in Tempeh Produced From Some Legumes and Jojobas Seeds

Authors: Ferial M. Abu-Salem, Rasha K. Mohamed, Ahmed Y. Gibriel, Nagwa M. H. Rasmy

Abstract:

Three legumes i.e. soybean, kidney bean and mung bean, and jojoba seed as an oil seed were processed into tempeh, a fermented food. Changes in phytic acid, total phenols and trypsin inhibitor were monitored during the pretreatments (soaking, soaking– dehulling, washing and cooking) and fermentation with Rhizopus oligosporus. Soaking was found to reduce total phenol and trypsin inhibitor levels in soybean, kidney bean and mung bean. However, phytic acid was reduced by soaking in kidney bean and mung bean. Cooking was the most effective in reducing the activity of trypsin inhibitor. During fermentation, a slight increase in the level of trypsin inhibitor was noticed in soybean. Phytic acid and total phenols were decreased during fermentation in soybean, kidney bean but mung bean faild to form tempeh because the antifungal activity of herein a protein in mung bean, which exerts both chitinase activity and antifungal activity against a variety of fungal species. On the other hand, solid- state fermentation of jojoba seeds was not effective in reducing their content from cyanogenic glycosides (simmondsin).

Keywords: Antinutritional factors, cyanogenic glycosides (Simmondsin), tempeh.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1093040

References:

[1] H. B, Elmaki, S. M. Abdel-Rahman, W. H. Idris, A. B. Hassan , E. E. Babiker, and A. H. El- Tinay, "Content of antinutritional factors and HCl-extractability of mineral from white bean (Phaseolus vulgaris). Cultivars: Influence of soaking and/or cooking," Food Chemistry, vol.100, pp. 362–368, 2007.
[2] A. Negi, P. Boora, and N. Khetarpaul, "Starch and protein digestibility of newly released moth bean cultivars: Effect of soaking, germination and pressure-cooking," Nahrung, vol.45, no. 4, pp. 251–254, 2001.
[3] A. Kamchan, P. Puwastien, P. P. Sirichakwal, and R. Kongkachuichai, "In vitro calcium bioavailability of vegetables, legumes and seeds," J. of Food Composition and Analysis, vol. 17, pp. 311–320, 2004.
[4] J. Das, Y. Chaturvedi, and R. Nagar, "Effect of germination on inorganic, labile and phytate phosphorous of some legumes," J. of Food Science and Technology, vol. 36, no. 6, pp. 532–534, 1999.
[5] P. Siddhuraju, K. Becker, and H. P. S. Makkar,. "Studies on the nutritional composition and antinutritional factors of three different germplasm seed materials of an underutilised tropical legume Mucuna pruriens var. utilis," J. of Agricultural and Food Chemistry, vol. 48, no. 12, pp.6048–6060, 2000.
[6] P. Siddhuraju, and K. Becker, "Effect of various domestic processing methods on antinutrients and in vitro protein and starch digestibility of two indigenous varieties of Indian tribal pulse, Mucuna pruriens var. utilis," J. of Agricultural Food Chemistry, vol.49, pp.3058–3067, 2001.
[7] V. Vidivel, and K. Janardhanen, "Nutritional and antinutrient attributes of the underutilized legume Cassia .oribunda car," Food Chemistry, vol.73, pp. 209–215, 2001.
[8] M. C. Olguin, N. Hisano, A. E. D’Ottavio, M. I. Zingale, G. C. Revelant, and S. A. Calderari, "Nutritional and antinutritional aspects of an Argenitian soy .our assessed on weanling rats," J. of Food Composition and Analysis, vol. 16, pp. 441–449, 2003.
[9] A. Nnanna, and R. D. Phillips, "Protein and starch digestibility and flatulence potential of germinated cowpeas (Vigna unguiculate)," J. Food Science, vol. 55, pp. 151–153, 1990.
[10] M. T. Al-Kaisey, T. R. Al-Hadithi, and A. K. H. Alwan, "Effect of germination on flatulence causing oligosaccharides in cowpeas (Vigna unguiculata)," Mu’tah J. Research Stud., vol. 11, no. 5, pp. 193–206, 1996.
[11] M. T. Al-Kaisey, T. R. Al-Hadithi, and B. A. A. Sahead, "Changes in vicine, convicine and oligosaccharides contents during germination of broad bean," Mu’tah J. Research Stud., vol. 12, no. 1, pp.327–345, 1997.
[12] S. Jood, U. Mehta, R. Singh, and C. M. Bhat, "Effect of processing on flatus-producing factors in legumes," J. of Agricultural and Food Chemistry, vol. 33, pp. 268– 271, 1985.
[13] C. Vidal-Valverde, J. Frias, I. Estrella, M. J. Gorospe, R. Ruiz, and J. Bacon, "Effect of processing on some antinutritional factors of lentils," J. Agricultural and Food Chemistry, vol. 42, pp. 2291-2295, 1994.
[14] S. Sefa-Dedh, D. W. Stanley, and P.W. Voisey, "Effect of storage time and conditions on the hard to cook defect in cowpeas (Vigna unguiculata)," J. Food Science, vol. 44, pp.790–796, 1979.
[15] G. Urbano, M. Lopez-Jurado, J. Hernandez, M. Fernandez, M. C. Frias, J. Moreu, C. Diaz-Pollan, M. Prodanov, and C. Vidal- Valverde, "Nutritional assessment of raw, heated, and germinated lentils," J. Agricultural and Food Chemistry, 43, pp. 1871- 1877, 1995.
[16] R. G. Zamora, and J. L. Veum, "The nutritive value of dehulled soybeans fermented with Aspergillus oryzae or Rhizopus oligosporus as evaluated by rats," J. Nutrition, vol. 109, pp. 1333–1338, 1979.
[17] N. R. Reddy, D. K. Salunkhe, and R. P. Sharma, "Flatulence in rats following ingestion of cooked and germinated black gram and a fermented product of black gram and rice blend," J. Food Science, vol. 45, pp. 1161–1164, 1980.
[18] E. K. Marfo, B. K. Simpson, J. S. Idowu, and O. L. Oke, "Effect of local food processing on phytate levels in cassava, cocoyam, yam, maize, sorghums, rice, cowpea and soybean," J. of Agricultural and Food Chemistry, vol. 38, pp. 1580–1585, 1990.
[19] R. Randhir, and K. Shetty, "Mung beans processed by solid-state bioconversion improve phenolic content and functionality relevant for diabetes and ulcer management," Innovative Food Science and Emerging Technologies, vol. 8, pp.197–204, 2007
[20] O. Paredes-López, and G. I. Harry, "Changes in selected chemical and antinutritional components during tempeh preparation using fresh and hardened common beans," J. Food Science, vol. 54, pp. 968-970, 1989.
[21] K. A. Hachmeister, and D. Y. C. Fung, "Tempeh: A Mold-modified indigenous fermented food made from soybeans and/or cereal grains," CRC Crit. Rev. Food Science Nutrition, vol. 19, pp. 137– 188, 1993.
[22] R. Sharma, and A. K. Sarbhoy, "Tempeh—a fermented food from soybean," Current Science, vol. 53, pp.325–326, 1984.
[23] APHA, "American Public Health Association. Compendium methods or microbiological examination for foods," pp.75-97, 239-250 and 325-420 Washington, D.C., USA, 1992.
[24] S. Suparmo, and P. Markakis, "Tempeh prepared from germinated soybeans: a research note," J. of Food Science, vol. 52, no. 6, pp.1736- 1737, 1987.
[25] M. Egounlety, "Production, properties and utilization of mould fermented foods from soybean (Glycine max Merr.), cowpea (Vigna unguiculata L. Walp) and groundbean (Macrotyloma geocarpa Harms)," Ph.D Thesis. University of Ibadan, Ibadan, Nigeria, 1994. (Cited from: Egounlety, M. and O.C. Arowh, 2003).
[26] A.O.A.C, "Official methods of analysis (17th Ed.). Gaithersburg, MD, USA," Association of Official Analytical Chemists, 2000.
[27] G. E. Hamerstrand, L. T. Black, and J. D. Glover, "Trypsin Inhibitors in Soy Products: Modification of the Standard Analytical Procedure," Cereal Chemistry, vol. 58, pp. 42-45, 1981.
[28] A. I. Mohamed, P. A. J. Perera, and Y. S. Hafez, "New Chromophore for phytic acid determination," Cereal Chemistry, vol. 63, pp. 475-476, 1986.
[29] S. F. Chandler, and J. H. Dodds, "The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum laciniatum," Plant Cell Rep., vol. 2, pp.105-108, 1983.
[30] K. Shetty, O. F. Curtis, R.E. Le vin, R. Witkowsky, and W. Ang, "Prevention of vitrification associated with in vitro shoot culture or oregano (Origanum vulgare) by Pseudonomas spp," J. Plant Physiol., vol. 47, pp.447-451, 1995.
[31] T. P. Abbott, R. A. Holser, B. J. Plattner, R. D. Plattner, and H. C.Purcell, "Pilot-scale isolation of simmondsin and related jojoba constituents," Ind. Crops Prod., vol. 10, pp. 65–72,1999.
[32] E. Y. Adreibigde, and A. O. Osegboun, "Acceptability of tempeh among health workers in Ado- Ekiti, Nigeria," Pakistan J. Nutrition, vol. 5, no.2, pp. 122-124, 2006.
[33] SAS. "SAS User’s Guide: Statistics (Version 5 Ed)," SAS Inst. Inc., Cary, NC, 1985.
[34] X. Ye, and T.B. Ng, "Chitinase with antifungal activity from the mung bean," Protein Expression and Purification, vol. 40, pp. 230–236, 2005.
[35] J. N. Hedger, "Production of tempeh, an Indonesian fermented foods the society for Genera Microbiology," pp.597-602, 1982.
[36] A. P. Vig, and A. Walia, "Beneficial Effects of Rhizopus Oligosporus Fermentation on Reduction of Glucosinolates, Fibre and Phytic Acid in Rapeseed (Brassica Napus) Meal," Bioresource Technology, vol. 78, no. 3, pp.309-312, 2001..
[37] S. M. M. Gadalla, "Effect of tempeh as a fermented food produced from some legumes on hypercholestrolemic rats," Ph.D. Thesis, Faculty of Specific Education, Ain Shams University, Egypt, pp.80-88, 2007.
[38] M. Egounlety, and O.C. Arowh, "Effect of soaking, dehulling, cooking and fermentation with Rhizopus oligosporus on the oligosaccharides, trypsin inhibitor, phytic acid and tannins of soybean (Glycine max Merr.), cowpea (Vigna unguiculata L. Walp) and groundbean (Macrotyloma geocarpa Harms)," J. of Food Engineering, vol. 56, pp. 249–254, 2003.
[39] A. Sharma, and S. Sehgal, "Effect of processing and cooking on the antinutritional factors of faba bean (Vicia faba)," Food Chemistry, vol. 43, pp. 383–385, 1992.