Preparation of POMA Nanofibers by Electrospinning and Its Applications in Tissue Engineering
Authors: Lu-Chen Yeh‚ Jui-Ming Yeh
In this manuscript, we produced neat electrospun poly(o-methoxyaniline) (POMA) fibers and utilized it for applying the growth of neural stem cells. The transparency and morphology of as-prepared POMA fibers was characterized by UV-visible spectroscopy and scanning electron microscopy, respectively. It was found to have no adverse effects on the long-term proliferation of the neural stem cells (NSCs), retained the ability to self-renew, and exhibit multipotentiality. Results of immunofluorescence staining studies confirmed that POMA electrospun fibers could provide a great environment for NSCs and enhance its differentiation.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1089461Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF
 N.C. Foulds, C.R. Lowe, Enzyme entrapment in electrically conducting polymers. Immobilisation of glucose oxidase in polypyrrole and its application in amperometric glucose sensors, J. Chem. Soc., Faraday Trans. 1 F, 82 (1986) 1259-1264.
 M. Umana, J. Waller, Protein-modified electrodes. The glucose oxidase/polypyrrole system, Anal. Chem., 58 (1986) 2979-2983.
 J.Y. Wong, R. Langer, D.E. Ingber, Electrically conducting polymers can noninvasively control the shape and growth of mammalian cells, Proc. Natl. Acad. Sci. U.S.A., 91 (1994) 3201-3204.
 G. Shi, M. Rouabhia, Z. Wang, L.H. Dao, Z. Zhang, A novel electrically conductive and biodegradable composite made of polypyrrole nanoparticles and polylactide, Biomaterials, 25 (2004) 2477-2488.
 A. Kotwal, C.E. Schmidt, Electrical stimulation alters protein adsorption and nerve cell interactions with electrically conducting biomaterials, Biomaterials, 22 (2001) 1055-1064.
 Y. Wan, H. Wu, D. Wen, Porous-Conductive Chitosan Scaffolds for Tissue Engineering, 1, Macromol. Biosci, 4 (2004) 882-890.
 P.M. George, A.W. Lyckman, D.A. LaVan, A. Hegde, Y. Leung, R. Avasare, C. Testa, P.M. Alexander, R. Langer, M. Sur, Fabrication and biocompatibility of polypyrrole implants suitable for neural prosthetics, Biomaterials, 26 (2005) 3511-3519.
 P.R. Bidez, S. Li, A.G. MacDiarmid, E.C. Venancio, Y. Wei, P.I. Lelkes, Polyaniline, an electroactive polymer, supports adhesion and proliferation of cardiac myoblasts, J. Biomater. Sci. Polym Ed, 17 (2006) 199-212.
 Y. Yang, Y. Min, J.C. Wu, D.J. Hansford, S.E. Feinberg, A.J. Epstein, Synthesis and Characterization of Cytocompatible Sulfonated Polyanilines, Macromol. Rapid Commun., 32 (2011) 887-892.
 Y. Liu, J. Hu, X. Zhuang, P. Zhang, Y. Wei, X. Wang, X. Chen, Synthesis and Characterization of Novel Biodegradable and Electroactive Hydrogel Based on Aniline Oligomer and Gelatin, Macromol. Biosci, 12 (2012) 241-250.
 S.H. Bhang, S.I. Jeong, T.-J. Lee, I. Jun, Y.B. Lee, B.-S. Kim, H. Shin, Electroactive Electrospun Polyaniline/Poly
[(L-lactide)- co-(ε-caprolactone)] Fibers for Control of Neural Cell Function, Macromol. Biosci, 12 (2012) 402-411.
 O. Lindvall, Z. Kokaia, A. Martinez-Serrano, Stem cell therapy for human neurodegenerative disorders–how to make it work, Nat Med, 10 (2004) S42-50.
 H. Cao, T. Liu, S.Y. Chew, The application of nanofibrous scaffolds in neural tissue engineering, Advanced Drug Delivery Reviews, 61 (2009) 1055-1064.
 L. Chen, Y. Yu, H. Mao, X. Lu, L. Yao, W. Zhang, Y. Wei, Synthesis of a new electroactive poly(aryl ether ketone), Polymer, 46 (2005) 2825-2829.
 Y. Furukawa, F. Ueda, Y. Hyodo, I. Harada, T. Nakajima, T. Kawagoe, Vibrational spectra and structure of polyaniline, Macromolecules, 21 (1988) 1297-1305.
 G. Kim, Y. Choe, J. Park, S. Cho, K. Kim, Activation of protein kinase A induces neuronal differentiation of HiB5 hippocampal progenitor cells, Molecular Brain Research, 109 (2002) 134-145.
 M.A. López-Toledano, C. Redondo, M.V.T. Lobo, D. Reimers, A.S. Herranz, C.L. Paíno, E. Bazán, Tyrosine Hydroxylase Induction by Basic Fibroblast Growth Factor and Cyclic AMP Analogs in Striatal Neural Stem Cells: Role of ERK1/ERK2 Mitogen-activated Protein Kinase and Protein Kinase C, Journal of Histochemistry & Cytochemistry, 52 (2004) 1177-1189.
 T. Zahir, Y.F. Chen, J.F. MacDonald, N. Leipzig, C.H. Tator, M.S. Shoichet, Neural Stem/Progenitor Cells Differentiate In Vitro to Neurons by the Combined Action of Dibutyryl cAMP and Interferon-γ, Stem Cells and Development, 18 (2009) 1423-1432.
 L.E. Fox, J. Shen, K. Ma, Q. Liu, G. Shi, G.D. Pappas, T. Qu, J. Cheng, Membrane Properties of Neuron-Like Cells Generated from Adult Human Bone-Marrow-Derived Mesenchymal Stem Cells, Stem Cells and Development, 19 (2010) 1831-1841.
 A.J.I. Roskams, X. Cai, G.V. Ronnett, Expression of neuron-specific beta-III tubulin during olfactory neurogenesis in the embryonic and adult rat, Neuroscience, 83 (1998) 191-200.
 E. Kawakita, M. Hashimoto, O. Shido, Docosahexaenoic acid promotes neurogenesis in vitro and in vivo, Neuroscience, 139 (2006) 991-997.