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Preparation of POMA Nanofibers by Electrospinning and Its Applications in Tissue Engineering

Authors: Lu-Chen Yeh‚ Jui-Ming Yeh

Abstract:

In this manuscript, we produced neat electrospun poly(o-methoxyaniline) (POMA) fibers and utilized it for applying the growth of neural stem cells. The transparency and morphology of as-prepared POMA fibers was characterized by UV-visible spectroscopy and scanning electron microscopy, respectively. It was found to have no adverse effects on the long-term proliferation of the neural stem cells (NSCs), retained the ability to self-renew, and exhibit multipotentiality. Results of immunofluorescence staining studies confirmed that POMA electrospun fibers could provide a great environment for NSCs and enhance its differentiation.

Keywords: Electrospun, polyaniline, neural stem cell, differentiation.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1089461

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References:


[1] N.C. Foulds, C.R. Lowe, Enzyme entrapment in electrically conducting polymers. Immobilisation of glucose oxidase in polypyrrole and its application in amperometric glucose sensors, J. Chem. Soc., Faraday Trans. 1 F, 82 (1986) 1259-1264.
[2] M. Umana, J. Waller, Protein-modified electrodes. The glucose oxidase/polypyrrole system, Anal. Chem., 58 (1986) 2979-2983.
[3] J.Y. Wong, R. Langer, D.E. Ingber, Electrically conducting polymers can noninvasively control the shape and growth of mammalian cells, Proc. Natl. Acad. Sci. U.S.A., 91 (1994) 3201-3204.
[4] G. Shi, M. Rouabhia, Z. Wang, L.H. Dao, Z. Zhang, A novel electrically conductive and biodegradable composite made of polypyrrole nanoparticles and polylactide, Biomaterials, 25 (2004) 2477-2488.
[5] A. Kotwal, C.E. Schmidt, Electrical stimulation alters protein adsorption and nerve cell interactions with electrically conducting biomaterials, Biomaterials, 22 (2001) 1055-1064.
[6] Y. Wan, H. Wu, D. Wen, Porous-Conductive Chitosan Scaffolds for Tissue Engineering, 1, Macromol. Biosci, 4 (2004) 882-890.
[7] P.M. George, A.W. Lyckman, D.A. LaVan, A. Hegde, Y. Leung, R. Avasare, C. Testa, P.M. Alexander, R. Langer, M. Sur, Fabrication and biocompatibility of polypyrrole implants suitable for neural prosthetics, Biomaterials, 26 (2005) 3511-3519.
[8] P.R. Bidez, S. Li, A.G. MacDiarmid, E.C. Venancio, Y. Wei, P.I. Lelkes, Polyaniline, an electroactive polymer, supports adhesion and proliferation of cardiac myoblasts, J. Biomater. Sci. Polym Ed, 17 (2006) 199-212.
[9] Y. Yang, Y. Min, J.C. Wu, D.J. Hansford, S.E. Feinberg, A.J. Epstein, Synthesis and Characterization of Cytocompatible Sulfonated Polyanilines, Macromol. Rapid Commun., 32 (2011) 887-892.
[10] Y. Liu, J. Hu, X. Zhuang, P. Zhang, Y. Wei, X. Wang, X. Chen, Synthesis and Characterization of Novel Biodegradable and Electroactive Hydrogel Based on Aniline Oligomer and Gelatin, Macromol. Biosci, 12 (2012) 241-250.
[11] S.H. Bhang, S.I. Jeong, T.-J. Lee, I. Jun, Y.B. Lee, B.-S. Kim, H. Shin, Electroactive Electrospun Polyaniline/Poly
[(L-lactide)- co-(ε-caprolactone)] Fibers for Control of Neural Cell Function, Macromol. Biosci, 12 (2012) 402-411.
[12] O. Lindvall, Z. Kokaia, A. Martinez-Serrano, Stem cell therapy for human neurodegenerative disorders–how to make it work, Nat Med, 10 (2004) S42-50.
[13] H. Cao, T. Liu, S.Y. Chew, The application of nanofibrous scaffolds in neural tissue engineering, Advanced Drug Delivery Reviews, 61 (2009) 1055-1064.
[14] L. Chen, Y. Yu, H. Mao, X. Lu, L. Yao, W. Zhang, Y. Wei, Synthesis of a new electroactive poly(aryl ether ketone), Polymer, 46 (2005) 2825-2829.
[15] Y. Furukawa, F. Ueda, Y. Hyodo, I. Harada, T. Nakajima, T. Kawagoe, Vibrational spectra and structure of polyaniline, Macromolecules, 21 (1988) 1297-1305.
[16] G. Kim, Y. Choe, J. Park, S. Cho, K. Kim, Activation of protein kinase A induces neuronal differentiation of HiB5 hippocampal progenitor cells, Molecular Brain Research, 109 (2002) 134-145.
[17] M.A. López-Toledano, C. Redondo, M.V.T. Lobo, D. Reimers, A.S. Herranz, C.L. Paíno, E. Bazán, Tyrosine Hydroxylase Induction by Basic Fibroblast Growth Factor and Cyclic AMP Analogs in Striatal Neural Stem Cells: Role of ERK1/ERK2 Mitogen-activated Protein Kinase and Protein Kinase C, Journal of Histochemistry & Cytochemistry, 52 (2004) 1177-1189.
[18] T. Zahir, Y.F. Chen, J.F. MacDonald, N. Leipzig, C.H. Tator, M.S. Shoichet, Neural Stem/Progenitor Cells Differentiate In Vitro to Neurons by the Combined Action of Dibutyryl cAMP and Interferon-γ, Stem Cells and Development, 18 (2009) 1423-1432.
[19] L.E. Fox, J. Shen, K. Ma, Q. Liu, G. Shi, G.D. Pappas, T. Qu, J. Cheng, Membrane Properties of Neuron-Like Cells Generated from Adult Human Bone-Marrow-Derived Mesenchymal Stem Cells, Stem Cells and Development, 19 (2010) 1831-1841.
[20] A.J.I. Roskams, X. Cai, G.V. Ronnett, Expression of neuron-specific beta-III tubulin during olfactory neurogenesis in the embryonic and adult rat, Neuroscience, 83 (1998) 191-200.
[21] E. Kawakita, M. Hashimoto, O. Shido, Docosahexaenoic acid promotes neurogenesis in vitro and in vivo, Neuroscience, 139 (2006) 991-997.