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Structured Phospholipids from Commercial Soybean Lecithin Containing Omega-3 Fatty Acids Reduces Atherosclerosis Risk in Male Sprague dawley Rats which Fed with an Atherogenic Diet
Abstract:Structured phospholipids from commercial soybean lecithin with oil enriched omega-3 fatty acid form by product of tuna canning is alternative procedure to provides the stability of omega-3 fatty acid structure and increase these bioactive function in metabolism. Best treatment condition was obtain in 18 hours acidolysis reaction with 30% enzyme concentration, which EPADHA incorporation level was 127,47 mg/g and incorporation percentage of EPA-DHA was 51,04% at phospholipids structure. This structured phospolipids could reduce atherosclerosis risk in male Sprague dawley rat. Provision of structured phospholipids has significant effect (α = 0.05) on changes in lipid profile, intima-media thickness of aorta rats (male Sprague dawley) fed atherogenic diet. Structured phospholipids intake can lower total cholesterol 78.36 mg/dL, total triglycerides 94,57 mg/dL, LDL levels 87.08 mg/dL and increased HDL level as much as 12,64 mg/dL in 10 weeks cares. Structured phospholipids intake also can prevent the thickening of the intima-media layer of the aorta.
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 Estiasih, T., K. Ahmadi, and F.C. Nisa. 2009. Synthesis Emulsifiers Containing -3 of Fatty Acids From The byproduct of Fish Processing and Oil Palm. Report Year II, Basic Research Incentives. Malang
 Lyberg, A-M., E. Fasoli, and P. Adlercreutz. 2005. Monitoring the oxidation of docosahexaenoic acid in lipids. J. Am. Oil Chem. Soc. 40: 969-979.
 Nur, M dan Estiasih, T. 2010. Synthesis of Soy Lecithin Phospholipids Containing Omega-3 Fatty Acids from Tuna Fish Canning by product. IMHERE - THP FTP UB. Malang
 Ahmadi, 2006. Optimization of Low Temperature Crystallization Solvent on the Preparation of Oil Rich -3 Fatty Acids from Fish Canning from byproduct of Lemuru Fish (Sardinella longiceps). Junior Lecturer Research Report. Malang
 Canas B. J and M.P. Yurawecz. 1999. Ethyl carbamate formation during urea complexation for fractination of fatty acid. JAOCS. Vol.76.No.4, 537
 Haraldsson, G.G. and A. Thorarensen. 1999. Preparation of phospholipids highly enriched with n-3 polyunsaturated fatty acids by lipase. J. Am. Oil Chem. Soc. 76(10): 1143-1149.
 Hromadov├í, M. et al. 1992. HMG-CoA reductase activity in the liver of rats with hereditary hypertriglyceridemia: effect of dietary fish oil. Biochem Cell Biol. 1992 Dec;70(12):1313-8
 Holub, B.J. 2002. Clinical nutrition: 4. Omega-3 fatty acids in cardiovascular care. JAMC ÔÇó 5 MARS 2002; 166 (5)
 Park, Seonhye and Park, Yongsoon. 2009. Effects of dietary fish oil and trans fat on rat aorta histopathology and cardiovascular risk markers. Nutrition Research and Practice (2009), 3(2), 102-107
 Navab. M. et al. 2003. Oral Synthetic Phospholipid (DMPC) Raises High-Density Lipoprotein Cholesterol Levels, Improves High-Density Lipoprotein Function, and Markedly Reduces Atherosclerosis in Apolipoprotein E-Null Mice . Circulation 2003, 108:1735-1739.
 Cohn, Jeffrey S. et al. 2008. Dietary phospholipids, hepatic lipid metabolism and cardiovascular disease. Current Opinion in Lipidology, 19:257-262.
 Nzai, J. M. and A. Proctor. 1998. Phospholipids determination in vegetable oil by thin layer chromatography and imaging densitometry. Food Chem. 63: 571-576
 Park, P.W. and R.E. Goins. 1994. In situ preparation of fatty acids methyl ester for analysis of fatty acids composition in foods. J. Food Sci. 59: 1262-1266.
 Jusi, D. 2004. Basics of Vascular Surgery. Faculty of Medicine. University of Indonesia. Jakarta
 Reeves, P.G., F. H. Nilson, and G. C. Fahey. 1993. Purified diet for laboratory rodents: final report of The American Institute of Nutrition Ad Hoc Writing Committee on The Reformulation of AIN-76 a Rodent Diet. J. Nutr. 123: 1939-1951.
 Maligan, J. M. et al. 2011. Hypocholesterolemic Effect of Yam (Dioscorea hispida Dennst) Flour in male Wistar rats fed a Hypercholesterolemia diet. Journal of Agricultural Technology. Vol. 12 No.2:91-98
 Cohn, J. S. et al. 2010. Reduction in intestinal cholesterol absorption by various food components: mechanisms and implications. Atherosclerosis Suppl. 2010 Jun; 11 (1):45-8.
 Iwata, T. et al. 1993. The effect of various phospholipids on plasma lipoproteins and liver lipids in hypercholesterolemic rats. J Nutr Sci Vitaminol (Tokyo). Feb; 39(1):63-71.
 Notarnicola, Maria. et al. 2010. Synergic effect of Eicosapentaenoic acid and Lovastatin on gene expression of HMGCoA reductase and LDL receptor in cultured HepG2 cells. Lipids in Health and Disease 2010, 9:135
 Davis P. J. 1992. n-3 and n-6 polyunsaturated fatty acids have different effects on acyl-CoA : cholesterol acyltransferase in J774 macrophages.
 Sugiyama, E. et al. 2008. Eicosapentaenoic acid lowers plasma and liver cholesterol levels in the presence of peroxisome proliferators-activated receptor alpha. Jul 4;83(1-2):19-28.
 Shirouci et al. 2007. Effect of Dietary Omega 3 Phosphatidylcholine on Obesity-Related Disorders in Obese Otsuka Long-Evans Tokushima Fatty Rats. J. Agric. Food Chem. 2007, 55, 7170-7176.
 Mastellone, I. et al. 2000. Dietary soybean phosphatidylcholines lower lipidemia: mechanisms at the levels of intestine, endothelial cell, and hepato-biliary axis. J.Nutr Biochem. Sep ; 11(9):461-6
 Leigh-Firbank, et al. 2002. Eicosapentaenoic acid and docosahexaenoic acid from fish oils: differential associations with lipid responses. British Journal of Nutrition (2002), 87, 435-445.
 Davidson, Michael H. 2006. Mechanisms for the Hypotriglyceridemic Effect of Marine Omega-3 Fatty Acids. American Journal of Cardiology. Volume 98, Issue 4, Supplement 1 , Pages 27-33, 21 August 2006.
 Mori, T.A., D.Q. Bao, V. Burke, I.E. Puddey, G.F. Watts, and L.J. Beilin. 1999. Dietary fish as a major component of a weight-loss diet: effect on serum lipids, glucose, and insulin metabolism in overweight hypertensive subjects. Am. J. Clin, Nutr. 70(5): 817-825.
 Das, Undurti N. 2008. Essential fatty acids and their metabolites could function as endogenous HMG-CoA reductase and ACE enzyme inhibitors, anti-arrhythmic, anti-hypertensive, anti-atherosclerotic, anti inflammatory, cytoprotective, and cardioprotective molecules. Lipids in Health and Disease 2008, 7:37
 Jimenez, M.A. et al. 1988. Evidence that Polyunsaturated Lecithin Induces a Reduction in Plasma Cholesterol Level and Favorable Changes in Lipoprotein Composition in Hypercholesterolemic Rats. J.Of. Nut. 0022-3166 : 659-677.
 Zuliani, et al. 2009. The Role of Polyunsaturated Fatty Acids (PUFA) in the Treatment of Dyslipidemias . Current Pharmaceutical Design. Volume 15 Issue 36.
 Pinkney, J. H. et al. 1999. Endothelial dysfunction in Type 1 DM mellitus: relationship with LDL oxidation and the effects of vitamin E. Diabet Med 16:993-999
 Upritchard, J. E. et al. 2000 Effect of supplementation with tomato juice vitamin E, and vitamin C on LDL oxidation and products of inflammatory activity in type 2 DM. DM Care 23:733-738
 Prasetyo, S and Sadhana, U. 2006. Cellular and Molecular Aspect of Atherosclerosis Medical Media (2). Jakarta
 Yu-Poth, Shaomei, et al. 2005. Long-Chain Polyunsaturated Fatty Acids Upregulate LDL Receptor Protein Expression in Fibroblasts and HepG2 Cells. Nutr. 135: 2541-2545.