Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30121
Structured Phospholipids from Commercial Soybean Lecithin Containing Omega-3 Fatty Acids Reduces Atherosclerosis Risk in Male Sprague dawley Rats which Fed with an Atherogenic Diet

Authors: Jaya Mahar Maligan, Teti Estiasih, Joni Kusnadi

Abstract:

Structured phospholipids from commercial soybean lecithin with oil enriched omega-3 fatty acid form by product of tuna canning is alternative procedure to provides the stability of omega-3 fatty acid structure and increase these bioactive function in metabolism. Best treatment condition was obtain in 18 hours acidolysis reaction with 30% enzyme concentration, which EPADHA incorporation level was 127,47 mg/g and incorporation percentage of EPA-DHA was 51,04% at phospholipids structure. This structured phospolipids could reduce atherosclerosis risk in male Sprague dawley rat. Provision of structured phospholipids has significant effect (α = 0.05) on changes in lipid profile, intima-media thickness of aorta rats (male Sprague dawley) fed atherogenic diet. Structured phospholipids intake can lower total cholesterol 78.36 mg/dL, total triglycerides 94,57 mg/dL, LDL levels 87.08 mg/dL and increased HDL level as much as 12,64 mg/dL in 10 weeks cares. Structured phospholipids intake also can prevent the thickening of the intima-media layer of the aorta.

Keywords: Structured phospholipids, commercial soybean lecithin, omega-3 fatty acid, atherosclerosis risk.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1073615

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2186

References:


[1] Estiasih, T., K. Ahmadi, and F.C. Nisa. 2009. Synthesis Emulsifiers Containing -3 of Fatty Acids From The byproduct of Fish Processing and Oil Palm. Report Year II, Basic Research Incentives. Malang
[2] Lyberg, A-M., E. Fasoli, and P. Adlercreutz. 2005. Monitoring the oxidation of docosahexaenoic acid in lipids. J. Am. Oil Chem. Soc. 40: 969-979.
[3] Nur, M dan Estiasih, T. 2010. Synthesis of Soy Lecithin Phospholipids Containing Omega-3 Fatty Acids from Tuna Fish Canning by product. IMHERE - THP FTP UB. Malang
[4] Ahmadi, 2006. Optimization of Low Temperature Crystallization Solvent on the Preparation of Oil Rich -3 Fatty Acids from Fish Canning from byproduct of Lemuru Fish (Sardinella longiceps). Junior Lecturer Research Report. Malang
[5] Canas B. J and M.P. Yurawecz. 1999. Ethyl carbamate formation during urea complexation for fractination of fatty acid. JAOCS. Vol.76.No.4, 537
[6] Haraldsson, G.G. and A. Thorarensen. 1999. Preparation of phospholipids highly enriched with n-3 polyunsaturated fatty acids by lipase. J. Am. Oil Chem. Soc. 76(10): 1143-1149.
[7] Hromadová, M. et al. 1992. HMG-CoA reductase activity in the liver of rats with hereditary hypertriglyceridemia: effect of dietary fish oil. Biochem Cell Biol. 1992 Dec;70(12):1313-8
[8] Holub, B.J. 2002. Clinical nutrition: 4. Omega-3 fatty acids in cardiovascular care. JAMC ÔÇó 5 MARS 2002; 166 (5)
[9] Park, Seonhye and Park, Yongsoon. 2009. Effects of dietary fish oil and trans fat on rat aorta histopathology and cardiovascular risk markers. Nutrition Research and Practice (2009), 3(2), 102-107
[10] Navab. M. et al. 2003. Oral Synthetic Phospholipid (DMPC) Raises High-Density Lipoprotein Cholesterol Levels, Improves High-Density Lipoprotein Function, and Markedly Reduces Atherosclerosis in Apolipoprotein E-Null Mice . Circulation 2003, 108:1735-1739.
[11] Cohn, Jeffrey S. et al. 2008. Dietary phospholipids, hepatic lipid metabolism and cardiovascular disease. Current Opinion in Lipidology, 19:257-262.
[12] Nzai, J. M. and A. Proctor. 1998. Phospholipids determination in vegetable oil by thin layer chromatography and imaging densitometry. Food Chem. 63: 571-576
[13] Park, P.W. and R.E. Goins. 1994. In situ preparation of fatty acids methyl ester for analysis of fatty acids composition in foods. J. Food Sci. 59: 1262-1266.
[14] Jusi, D. 2004. Basics of Vascular Surgery. Faculty of Medicine. University of Indonesia. Jakarta
[15] Reeves, P.G., F. H. Nilson, and G. C. Fahey. 1993. Purified diet for laboratory rodents: final report of The American Institute of Nutrition Ad Hoc Writing Committee on The Reformulation of AIN-76 a Rodent Diet. J. Nutr. 123: 1939-1951.
[16] Maligan, J. M. et al. 2011. Hypocholesterolemic Effect of Yam (Dioscorea hispida Dennst) Flour in male Wistar rats fed a Hypercholesterolemia diet. Journal of Agricultural Technology. Vol. 12 No.2:91-98
[17] Cohn, J. S. et al. 2010. Reduction in intestinal cholesterol absorption by various food components: mechanisms and implications. Atherosclerosis Suppl. 2010 Jun; 11 (1):45-8.
[18] Iwata, T. et al. 1993. The effect of various phospholipids on plasma lipoproteins and liver lipids in hypercholesterolemic rats. J Nutr Sci Vitaminol (Tokyo). Feb; 39(1):63-71.
[19] Notarnicola, Maria. et al. 2010. Synergic effect of Eicosapentaenoic acid and Lovastatin on gene expression of HMGCoA reductase and LDL receptor in cultured HepG2 cells. Lipids in Health and Disease 2010, 9:135
[20] Davis P. J. 1992. n-3 and n-6 polyunsaturated fatty acids have different effects on acyl-CoA : cholesterol acyltransferase in J774 macrophages.
[21] Sugiyama, E. et al. 2008. Eicosapentaenoic acid lowers plasma and liver cholesterol levels in the presence of peroxisome proliferators-activated receptor alpha. Jul 4;83(1-2):19-28.
[22] Shirouci et al. 2007. Effect of Dietary Omega 3 Phosphatidylcholine on Obesity-Related Disorders in Obese Otsuka Long-Evans Tokushima Fatty Rats. J. Agric. Food Chem. 2007, 55, 7170-7176.
[23] Mastellone, I. et al. 2000. Dietary soybean phosphatidylcholines lower lipidemia: mechanisms at the levels of intestine, endothelial cell, and hepato-biliary axis. J.Nutr Biochem. Sep ; 11(9):461-6
[24] Leigh-Firbank, et al. 2002. Eicosapentaenoic acid and docosahexaenoic acid from fish oils: differential associations with lipid responses. British Journal of Nutrition (2002), 87, 435-445.
[25] Davidson, Michael H. 2006. Mechanisms for the Hypotriglyceridemic Effect of Marine Omega-3 Fatty Acids. American Journal of Cardiology. Volume 98, Issue 4, Supplement 1 , Pages 27-33, 21 August 2006.
[26] Mori, T.A., D.Q. Bao, V. Burke, I.E. Puddey, G.F. Watts, and L.J. Beilin. 1999. Dietary fish as a major component of a weight-loss diet: effect on serum lipids, glucose, and insulin metabolism in overweight hypertensive subjects. Am. J. Clin, Nutr. 70(5): 817-825.
[27] Das, Undurti N. 2008. Essential fatty acids and their metabolites could function as endogenous HMG-CoA reductase and ACE enzyme inhibitors, anti-arrhythmic, anti-hypertensive, anti-atherosclerotic, anti inflammatory, cytoprotective, and cardioprotective molecules. Lipids in Health and Disease 2008, 7:37
[28] Jimenez, M.A. et al. 1988. Evidence that Polyunsaturated Lecithin Induces a Reduction in Plasma Cholesterol Level and Favorable Changes in Lipoprotein Composition in Hypercholesterolemic Rats. J.Of. Nut. 0022-3166 : 659-677.
[29] Zuliani, et al. 2009. The Role of Polyunsaturated Fatty Acids (PUFA) in the Treatment of Dyslipidemias . Current Pharmaceutical Design. Volume 15 Issue 36.
[30] Pinkney, J. H. et al. 1999. Endothelial dysfunction in Type 1 DM mellitus: relationship with LDL oxidation and the effects of vitamin E. Diabet Med 16:993-999
[31] Upritchard, J. E. et al. 2000 Effect of supplementation with tomato juice vitamin E, and vitamin C on LDL oxidation and products of inflammatory activity in type 2 DM. DM Care 23:733-738
[32] Prasetyo, S and Sadhana, U. 2006. Cellular and Molecular Aspect of Atherosclerosis Medical Media (2). Jakarta
[33] Yu-Poth, Shaomei, et al. 2005. Long-Chain Polyunsaturated Fatty Acids Upregulate LDL Receptor Protein Expression in Fibroblasts and HepG2 Cells. Nutr. 135: 2541-2545.