Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30172
Function of miR-125b in Zebrafish Neurogenesis

Authors: Minh T. N. Le, Cathleen Teh, Ng Shyh-Chang, Vladimir Korzh, Harvey F. Lodish, Bing Lim

Abstract:

MicroRNAs are an important class of gene expression regulators that are involved in many biological processes including embryogenesis. miR-125b is a conserved microRNA that is enriched in the nervous system. We have previously reported the function of miR-125b in neuronal differentiation of human cell lines. We also discovered the function of miR-125b in regulating p53 in human and zebrafish. Here we further characterize the brain defects in zebrafish embryos injected with morpholinos against miR-125b. Our data confirm the essential role of miR-125b in brain morphogenesis particularly in maintaining the balance between proliferation, cell death and differentiation. We identified lunatic fringe (lfng) as an additional target of miR-125b in human and zebrafish and suggest that lfng may mediate the function of miR-125b in neurogenesis. Together, this report reveals new insights into the function of miR- 125b during neural development of zebrafish.

Keywords: microRNA, miR-125b, neurogenesis, zebrafish.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1073124

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1380

References:


[1] D. P. Bartel, "MicroRNAs: genomics, biogenesis, mechanism, and function," Cell, vol. 116, pp. 281-297, 2004.
[2] B. Ason, et al., "From the Cover: Differences in vertebrate microRNA expression," Proc.Natl.Acad.Sci.U.S.A, vol. 103, pp. 14385-14389, 2006.
[3] M. Selbach, et al., "Widespread changes in protein synthesis induced by microRNAs," Nature, vol. 455, pp. 58-63, 2008.
[4] P. H. Olsen and V. Ambros, "The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation," Dev.Biol., vol. 216, pp. 671-680, 1999.
[5] E. E. Caygill and L. A. Johnston, "Temporal regulation of metamorphic processes in Drosophila by the let-7 and miR-125 heterochronic microRNAs," Curr.Biol., vol. 18, pp. 943-950, 2008.
[6] E. A. Miska, et al., "Microarray analysis of microRNA expression in the developing mammalian brain," Genome Biol., vol. 5, p. R68, 2004.
[7] L. Smirnova, et al., "Regulation of miRNA expression during neural cell specification," Eur.J.Neurosci., vol. 21, pp. 1469-1477, 2005.
[8] L. F. Sempere, et al., "Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation," Genome Biol., vol. 5, p. R13, 2004.
[9] E. Wienholds, et al., "MicroRNA expression in zebrafish embryonic development," Science, vol. 309, pp. 310-311, 2005.
[10] M. T. Le, et al., "MicroRNA-125b promotes neuronal differentiation in human cells by repressing multiple targets," Mol Cell Biol, vol. 29, pp. 5290-305, Oct 2009.
[11] M. T. Le, et al., "MicroRNA-125b is a novel negative regulator of p53," Genes Dev, vol. 23, pp. 862-76, Apr 1 2009.