Characterization of Novel Atrazine-Degrading Klebsiella sp. isolated from Thai Agricultural Soil
Authors: Sawangjit Sopid
Atrazine, a herbicide widely used in sugarcane and corn production, is a frequently detected groundwater contaminant. An atrazine-degrading bacterium, strain KB02, was obtained from long-term atrazine-treated sugarcane field soils in Kanchanaburi province of Thailand. Strain KB02 had a rod-to-coccus morphological cycle during growth. Sequence analysis of the PCR product indicated that the 16S rRNA gene in strain KB02 was ranging from 97-98% identical to the same region in Klebsiella sp. Based on biochemical, physiological analysis and 16S rDNA sequence analysis of one representative isolate, strain KB02, the isolates belong to the genus Klebsiella in the family Enterobacteriaceae. Interestingly that the various primers for atzA, B and C failed to amplify genomic DNA of strain KB02. Whereas the expected PCR product of atzA, B and C were obtained from the reference strain, Arthrobacter sp. strain KU001.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1073076Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1545
 U.S. Environmental Protection Agency, Rules and regulations.Fed. Regist, 56:3552, 1991.
 A.M. Cook, "Biodegradation of s-triazine xenobiotics," FEMS Microbiol.Rev,vol. 46, pp.93-116, 1987.
 E.L. Erickson, and K.H. Lee, "Degradation of atrazine and relatedstriazines," Crit. Rev. Environ. Contam, vol. 19, pp.1-13, 1989.
 R.W. Eaton, and J.S. Karns, "Cloning and comparison of the DNA encoding ammelide aminohydrolase and cyanuric acid amidohydrolase from three s-triazine-degrading bacterial strain," J. Bacteriol, vol. 173, pp.1363-1366, 1991.
 C.Yanze-Kontchou, and N. Gschwind, "Mineralization of the herbicide atrazine as a carbon source by a Pseudomonas strain," Appl.Environ. Microbiol, vol. 60, pp. 4297-4302, 1994.
 C. Bouquard, J. Ouazzani, J.C. Prome, Y. Michel-Briand, and P. Plesiat,"Dechlorination of atrazine by a Rhizobium sp. Isolate," Appl Environ Microbiol, vol. 63, pp. 862-866, 1997.
 L.C. Strong, C. Rosendahl, G. Johnson, M.J. Sadowsky, and L.P. Wackett, "Arthrobacter aurescens TC1 metabolizes diverse s-triazine ring compounds," Appl Environ Microbiol, vol. 68, pp.5973-5980,2002.
 R.T. Mandelbaum, D.L. Allan, and L.P. Wackett, "Isolation and characterization of a Pseudomonas sp. that mineralizes the s-triazine herbicide atrazine," Appl Environ Microbiol, vol. 61, pp.1451-1457, 1995.
 K.L. Boundy-Mills, M.L. de Souza, R.T. Mandelbaum, L.P. Wackett, And M.J. Sadowsky, "The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway," Appl Environ Microbiol, vol. 63, pp. 916-923, 1997.
 M.L. de Souza, M.J. Sadowsky, and L.P. Wackett, "Atrazine Chlorohydrolase from Pseudomonas sp. Strain ADP:Gene Sequence, Enzyme Purification, and Protein Characterization," J Bacteriol, vol. 178, pp. 4894-4900, 1996.
 M.J. Sadowsky, Z. Tong, M. de Souza, L.P. Wackett, "AtzC is a new member of the amidohydrolase protein superfamily and is homologous to other atrazine-metabolizing enzymes," J Bacteriol, vol. 180, pp. 152- 158, 1998.
 R.W. Eaton, and D.W. Ribbons, "The metabolism of dimethylphthalate by Micrococcus sp. strain 12B," J Bacteriol, vol. 151, pp. 465-467, 1982.
 J. Sambrook, E.F. Fritsch, and T. Maniatis, "Molecular Cloning: A Laboratory Manual," Cold Spring Harbor Laboratory, New York, 545 p, 1989.
 J.D. Thompson, D.G. Higgins, and T.J. Gibson, "CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice," Nucleic Acids Res, vol. 22, pp. 4673-4680, 1994.