Sawangjit Sopid
Characterization of Novel AtrazineDegrading Klebsiella sp. isolated from Thai Agricultural Soil
618 - 620
2012
6
8
International Journal of Agricultural and Biosystems Engineering
https://publications.waset.org/pdf/9601
https://publications.waset.org/vol/68
World Academy of Science, Engineering and Technology
Atrazine, a herbicide widely used in sugarcane and corn production, is a frequently detected groundwater contaminant. An atrazinedegrading bacterium, strain KB02, was obtained from longterm atrazinetreated sugarcane field soils in Kanchanaburi province of Thailand. Strain KB02 had a rodtococcus morphological cycle during growth. Sequence analysis of the PCR product indicated that the 16S rRNA gene in strain KB02 was ranging from 9798 identical to the same region in Klebsiella sp. Based on biochemical, physiological analysis and 16S rDNA sequence analysis of one representative isolate, strain KB02, the isolates belong to the genus Klebsiella in the family Enterobacteriaceae. Interestingly that the various primers for atzA, B and C failed to amplify genomic DNA of strain KB02. Whereas the expected PCR product of atzA, B and C were obtained from the reference strain, Arthrobacter sp. strain KU001.
Open Science Index 68, 2012