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Effect of Dietary Supplementation of Different Levels of Black Seed (Nigella Sativa L.) on Growth Performance, Immunological, Hematological and Carcass Parameters of Broiler Chicks
Abstract:This experiment was conducted to investigate the effect of dietary supplementation of different levels of black seed (Nigella sativa L.) on the performance and immune response of broiler chicks. A total 240 day-old broiler chicks were used and randomly allotted equally into six experimental groups designated as 1, 2, 3, 4, 5 and 6 having black seed at the rate of 0, 2, 4, 6, 8 and 10 g /kg diet respectively. The study was lasted for 42 days. Average body weight, weight gain, relative growth rate, feed conversion, antibody titer against Newcastle disease, phagocytic activity and phagocytic index, some blood parameters(GOT, GPT, Glucose, Cholesterol, Triglyceride, Total protein, Albumen, WBCs, RBCs, Hb and PCV), dressing percentage, weight of different body organs, abdominal fat weight, were determined. It was found that, N. Sativa significantly improved final body weight, total body gain and feed conversion ratio of groups 2 and 3 when compared with the control group. Higher levels of N. Sativa did not improve growth performance of the chicks. Non significant differences were observed for antibody titer against Newcastle virus, WBCs count, serum GOT, glucose level, dressing %, relative liver, spleen, heart and head percentages. Lymphoid organs (Bursa and Thymus) improved significantly with increasing N. Sativa level in all supplemented groups. Serum cholesterol, triglyceride and visible fat % significantly decreased with Nigella sativa supplementation while serum GPT level significantly increased with nigella sativa supplementation.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1072271Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2516
 A. A. H. Tollbaand M. S. H. Hassan "Using some natural additives to improve physiological and productive performance of broiler chicks under high temperature conditions 2- black cumin (Nigella Sativa) or garlic (Allium sativum) ," Egypt. Poul. Sci. 2003, 23 (II): (327-340).
 T. Guler, B. Dalkilic, O. N. Ertas, and M. Ciftci"The effect of dietary black cumin seeds (Nigella Sativa L.) on the performance of broilers," Asian-australasian journal of animal sciences. 2006, 19:3, 425-430.
 F. R. Durrani, N. Chand , K. Zaka, A. Sultan , F. M. Khattak, and Z. Durrani "Effect of different levels of feed added black seed (Nigella sativa L.) On the performance of broiler chicks," Pak J Biol Sci. 2007, Nov 15; 10 (22):4164-7.
 A. A. Abdel-Hady, F. A. Abdel-Azeem, A. A. Abdel-Rafea, and A. G. Gamal "Effect of replacement of soybean meal protein by nigella sativa meal protein on performance of growing Japanese quail. Egypt,"Poult. Sci. 2009, Vol (29) (I): (407-422).
 S. Mahmood, M. M. Hassan, M. Alam, and F. Ahmad "Comparative efficacy of Nigella sativa and Allium sativum as growth promoters in broilers," Int. J. Agric. Biol., 2009, 11: 775-778
 ├ç. Mehmet, Y. Black Seed Extract (Nigella sativa) on Growth Performance, Blood Parameters, Oxidative Stress and DNA Damage of Partridges," Journal of Applied Animal Research,2008,Volume: 34, Issue : 2 Print ISSN : 0971-2119. Online ISSN: 0974-1844.
 Y. A. Attia, A. E. Tag El-Din, H. S. Zeweil, A. S. Hussein, E. M. Qota, and M. A. Arafat "The effect of supplementation of enzyme on laying and reproductive performance in Japanese quail hens fed nigella seed meal," Journal of Poultry Science.2008, 45:110-115.
 E. S. Abd El-Aal, and R. S. Attia"Characterization of black cumin (Nigella sativa), chemical composition and lipids," Alex. Sci. Exch. 1993, 14(4): 467-481.
 W. A. James, E. J. Amy, and R. M. Susan "Ten different dietary fibers have significantly different effects on serum and liver lipids of cholesterol-fed rats," J. Nutri. 1994, 124: 78-83.
 S. Hedaya,"Effects of Nigella sativa (balck seeds) extracts on some hematological and biochemical parameters in rats," Alex. J. Vet. Sci. 1995, 11(2): 95-99.
 N. A. AL-Beitawi, S. S. EL-Ghousein, and A. H. Nofal "Replacing bacitracin methylene disalicylate by crushed Nigella sativa seeds in broiler rations and its effects on growth, blood constituents and immunity,"Livestock Science. 2009, Volume 125, Issues 2-3, Pages 304-307.
 M. Toghyani, M. Toghyani, A. Gheisari, G. Ghalamkari, and M. Mohammadrezaei"Growth performance, serum biochemistry and blood hematology of broiler chicks fed different levels of black seed (Nigella sativa) and peppermint (Menthapiperita)," Livestock Science.2010,129, 173-178
 A. Al-Homidan, A. Al-Qarawi, S. A. Al-Waily, and S. E. Adam"Response of broiler chicks to dietary Rhazyastricta and Nigella sativa,"Br Poult Sci.2002, 43(2):291-6.
 M. S. H. Hassan, A. M. AboTaleb, M.M. Wakwak, and B.A. Yousef "Productive, physiological and immunological effect of using some natural feed additives in Japanese quail,"Egypt.Poul.Sci. 2007, 27(II):557-581.
 NRC,"National Research Council: Nutrient requirements of poultry,"1994, 9thed National Academy press, Washington, DC.
 E. W. Crampton, and L. E. Lioyd"Fundamentals of Nutrition,"1959, W. H. Free and Company. San Francisco and London.
 W. V. Lambert, N. R. Ellis, W. H. Block, and H. W. Titus "The role of nutrition in genetics," Am. Res. Soc Animal prod. 1936, 29: 236. ¶ÇÇà¶ÇÇü¶ÇÇé¶ÇÇå G. Y. Takatasy,"The use of spiral 100 Min Serological and Virologicalmicromethods,"Acta, Microbial, Acad. Sci. Hung. 1955, 3:191. ¶ÇÇà¶ÇÇü¶ÇÇä¶ÇÇå M. G. Brugh, "A simple method for recording and analyzing serological data," Avian Disease.1978, 22: 362-365.
 E. Kawahara, T. Ueda, and S. Nomura "In vitro phagocytic activity of white spotted shark cells after injection with Aeromoassalmonicidaextraceular products,"Gyobyo ,Kenkyu, Japan, 1991, 26(4):213-214.
 S. Reitman, and S. Frankel "A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase," Am. J. Path. 1957, 26: 1-13.
 P. Trinder, "Determination of glucose in blood using glucose oxidase with alternative oxygen acceptor," Ann. Clin. Biochm. 1969, 6: 24.
 B. Zak, R.Dickenman, E. White, H. Burnet, and P. Cherney"Rapid estimation of free and total cholesterol," Am. J. Clin. Path. 1954, 24: 1307.
 P. G. Sidney, and R. Barnard "Improved manual spectrophotometric procedure for determination of serum triglycerides,"Clin. Chem. 1973, 19 (9): 1077-1078.
 B. T.Doumas, D. D. Bayso, R. J. Carter, T. Peters, and R. Schaffer "Determination of total serum protein,"Clin. Chem. 1981, 27:1642- 1643.
 R. R. Reinhold, "Determination of serum albumin,"Clin. Chem. 1953, 21: 1370 -1372.
 M. Maxine, and B. S. Benjamin "Outline of veterinary clinical pathology,"1985, 3rd Ed., Colorado State University.
 Z. Lucky, "Methods for diagnosis of fish disease,"Ameruno publishing Co, PVT, Ltd New Delhi, Bomby, New York, 1977.
 Statistical Analysis System (SAS) "Statistical user-s Guide,".2004, INT., Cary, NC. USA.
 H. M. Ziad, Abu-Dieyeh, and S. A. Mohammad "Effect of feeding powdered Black Cumin Seeds (Nigella Sativa L.) on growth performance of 4- weeks- old Broilers," Journal of Animal and Veterinary Advances. 2008, 7, (3): 286-290.
 G. Erener, N. Altop, H. Ocak, S. Aksoy, and E. Ozturk,"Influence of black cumin seed (Nigella sativa L.) and seed extract on broilers performance and total coliform bacteria count," Asian J. Anim. Vet. Adv. 2010, 5: 128-135.
 N. I. Hassanin, and F.M. Hassan,"A preliminary study on the effect of Nigella sativa L.seeds on hypoglycemia," Vet. Med. J. Giza.1996, 44:4, 699-708.
 M. El-Dakhakhny, N. Mady, N. Lembert, and H.P.T. Ammon,"The hypoglycemic effect of Nigella sativa oil is mediated by extrapancreatic actions,". Plant. Medica. 2002, 68: 5, 465-466;6 ref.
 A. Zaoui, Y.P.Cherrah,K. Alaoui, N. Mahassine, L.T. Amarouch, and M. Hassar,"Effectes of Nigella sativa fixed oil on blood homeostasis in rat,"J.Ethnopharmacol Jan. 2002; 79:1, 23-26.
 K. M. Fararh, Y. Atoji, Y. Shimizu, and T. Takewaki,"Insulinotropic properties of Nigella sativa oil in streptozotocin plus Nicotinamiede diabetic hamster,". Research in Veterinary Science. 2002,73: 3, 279-282.
 I. Meral, N. Donmez, B. Baydas, F. Belge, and M. Kanter,"Effect of Nigella sativa L. on heart rate and some haematological values of alloxan induced diabetic rabbits," Scandinavian Journal of Laboratory Animal science. 2004, 31: 1, 49-53.
 N. A.AL-Beitawi, and S.S. El-Ghousein,"Effect of Feeding Different Levels of Nigella sativa Seeds (Black Cumin) on Performance, Blood Constituents and Carcass Characteristics of Broiler Chicks," International Journal of Poultry Science.2008, 7,(7): 715-721.