Commenced in January 2007
Paper Count: 30172
Optimization of Gentamicin Production: Comparison of ANN and RSM Techniques
Abstract:In this work, statistical experimental design was applied for the optimization of medium constituents for Gentamicin production by Micromsonospora echinospora subs pallida (MTCC 708) in a batch reactor and the results are compared with the ANN predicted values. By central composite design, 50 experiments are carried out for five test variables: Starch, Soya bean meal, K2HPO4, CaCO3 and FeSO4. The optimum condition was found to be: Starch (8.9,g/L), Soya bean meal (3.3 g/L), K2HPO4 (0.8 g/L), CaCO3 (4 g/L) and FeSO4 (0.03 g/L). At these optimized conditions, the yield of gentamicin was found to be 1020 mg/L. The R2 values were found to be 1 for ANN training set, 0.9953 for ANN test set, and 0.9286 for RSM.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1070563Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1375
 M. J. Weinstein, G.M. Luedemann, E.M. Oden, G.H. Wagman, J.P. Rosselet, J.A. Marquez, C.T. Coniglio, W. Charney, H.L. Herzog, and J. Black, "Gentamicin, new antibiotic complex from Micromonospora", J. Med. Chem., vol. 6, 1963, pp. 463-464.
 J. Chu, S. Zhang, Y. Zhuang, J. Chen, and Y. Li, "Factors affecting the biosynthesis and secretion of gentamicin", Process Biochem., vol.38, 2002, pp. 815-820.
 J. Chu, W. Niu, S. Zhang, Y. Zhuang, H. Hu, and Y. Li, "Effect of metal ions on the binding of gentamicin to the peptidoglycan of Micromonospora echinospora, Process Biochem, vol.39, 2004, pp.1145- 1150.
 M. Himabindu, R. Potumarthi, and A. Kitty, "Enhancement of gentamicin production by mutugenesis and non nutritional stress conditions in Micromonospora echinospora". Process Biochem, vol.42, 2007, pp. 1352-1356.
 M. Himabindu, P. Ravichandra, and J. Annapurna, "Evaluation of immobilization conditions for enhanced production of gentamicin in repeated batch operation by micromonospora echinospora"Int.J.of chemical Reaction Engineering, vol.5, 2007, pp. 1-13.
 M. Himabindu, P. Ravichandra, and J. Annapurna, "Gentamicin production by micromonospora echinospora (Me-22) in stirred tank reactor:effectof various parameters:" Journal of basic microbiology vol.48, 2007, pp. 53-58.
 K. Mehrotra, C.K. Mohan, and S. Ranka, S., "Elements of Artificial Neural Networks", Penram International, Mumbai, 1997
 L. Govindarajan, Radial Basis Neural Network Approach to VLE Data Predictions, M.E., Dissertation, Annamalai University, India, 2002.
 Y.C.Chang, C.L. Lee, and T.M. Pan, "Statistical optimization of media components for the production of Antrodia cinnamomea AC0623 in submerged cultures", Applied Microbiology and Biotechnology, vol.72, 2006, pp. 654-661.
 E.Kristo, C.G. Biliaderis, and N. Tzanetakis, "Modeling of the acidification process and rheological properties of milk fermented with a yogurt starter culture using response surface methodology", Food Chemistry, vol. 83, 2003, pp. 437 - 446.
 Q.K. Beg, R.K. Saxena, and R. Gupta, "Kinetic constants determination for an alkaline protease from Bacillus mojavensis using response surface methodology", Biotechnol. Bioeng. vol.78, 2002, pp. 289-295.
 L.S.T.Lai, C.C. Pan, and B.K. Tzeng, "The influence of medium design on lovastatin production and pellet formation with a high-producing mutant of Aspergillus terreus in submerged cultures", Process Biochemistry, vol.38, 2003, pp. 1317 - 1326.
 E.L.Soo, A.B. Salieh and M. Basri, "Response surface methodological study on lipase-catalyzed synthesis of amino acid surfactants", Process Biochemistry, vol.39, 2004, pp. 1511 - 1518.
 Y.X.Wang, and Z.X. Lu, "Optimization of processing parameters for the mycelial growth and extracellular polysaccharide production by Boletus spp. ACCC 50328", Process Biochemistry, vol.40, 2005, pp. 1043 - 1051.
 H. Wang, J. Ren, and Y. Zhang, "Use of p-dimethylaminobenzalhyde as a coloured reagent for determination of gentamycin", Talanta, Vol. 40, 1993, pp.851-853
 L. Govindarajan, "Optimal design of reactors", Ph D Thesis, Annamalai University, India, 2005.