Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30075
Enhancing Efficiency for Reducing Sugar from Cassava Bagasse by Pretreatment

Authors: S. Gaewchingduang, P. Pengthemkeerati

Abstract:

Cassava bagasse is one of major biomass wastes in Thailand from starch processing industry, which contains high starch content of about 60%. The object of this study was to investigate the optimal condition for hydrothermally pretreating cassava baggasses with or without acid addition. The pretreated samples were measured reducing sugar yield directly or after enzymatic hydrolysis (alpha-amylase). In enzymatic hydrolysis, the highest reducing sugar content was obtained under hydrothermal conditions for at 125oC for 30 min. The result shows that pretreating cassava baggasses increased the efficiency of enzymatic hydrolysis. For acid hydrolysis, pretreating cassava baggasses with sulfuric acid at 120oC for 60 min gave a maximum reducing sugar yield. In this study, sulfuric acid had a greater capacity for hydrolyzing cassava baggasses than phosphoric acid. In comparison, dilute acid hydrolysis to provide a higher yield of reducing sugar than the enzymatic hydrolysis combined hydrothermal pretreatment. However, enzymatic hydrolysis in a combination with hydrothermal pretreatment was an alternative to enhance efficiency reducing sugar production from cassava bagasse.

Keywords: Acid hydrolysis, cassava bagasse, enzymatic hydrolysis, hydrothermal pretreatment.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1070281

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2447

References:


[1] Office of Agricultural Economics, "Agricultural Import Export 2552," Ministry of Agriculture and Cooperatives, Bangkok, Thailand, 2553.
[2] K. Sriroth, R. Chollakup, S. Chotineeranat, K. Piyachomkwan and C.G. Oates,"Processing of cassava waste for improved biomass utilization," Bioresource technology, vol 71, pp. 63-69, 2000.
[3] A. Kosugi, A. Kondo, M. Ueda, Y. Murata, P. Vaithanomsat, W. Thanapase, T. Arai and Y. Mori, "Production of ethanol from cassava pulp via fermentation with a surface engineered yeast strain displaying glucoamylase," Renewable Energy, vol 34, pp. 1354-1358, 2009.
[4] U. Rattanachomsri, S. Tanapongpipat, L. Eurwilaichitr and V. Champreda, "Simultaneous non-thermal saccharification of cassava pulp by multienzyme activity and ethanol fermentation by Candida tropicalis," Journal of Bioscience and Bioengineering, vol 107, pp. 488-493, 2009.
[5] A. Pandey, C.R. Soccol, P. Nigam, V.T. Soccol, L. P.S. Vandenberghe and R. Mohan, "Review paper: Biotechnological potential of agro-industrial residues. II: cassava bagasse," Bioresource technology, vol 74, pp. 81-87, 2000.
[6] H. Inoue, S. Yano, T. Endo, T. Sakaki and S. Sawayama, "Research: Combining hot-compressed water and ball milling pretreatments to improve the efficiency of the enzymatic hydrolysis of eucalyptus," Biotechnology for Biofuels, vol 1, Apr. 2008.
[7] N. Mosier, C. Wyman, B. Dale, R. Elander, Y.Y. Lee, M. Holtzapple and M. Ladish, "Features of promising technologies for pretreatment of lignocellulosic biomass," Bioresource Technology, vol 96, pp. 673-686, Sep. 2005.
[8] M. Galbe and G. Zacchi, "A review of the production of ethanol from softwood," Applied microbiology and biotechnology, vol 59, pp. 618- 628, 2002.
[9] M. Linde, M. Galbe and G. Zacchi, "Bioethanol production from nonstarch carbohydrate residues in process streams from a dry-mill ethanol plant," Bioresource Technology, vol 99, pp. 6505-6511, Feb. 2008.
[10] I. Cybulska, H. Lei and J. Julson, "Hydrothermal pretreatment and enzymatic hydrolysis of prairie cord grass," Energy Fuels, vol 24, pp. 718- 727, 2010.
[11] M.J. Negro, P. Manzanares, I. Ballesteros, J.M. Oliva, A. Cabanas and M. Ballesteros, "Hydrothermal pretreatment conditions to enhance ethanol production from poplar biomass," Applied Biochemistry and Biotechnology, vol 105-108, pp. 87-100, 2003.
[12] J.D. Wright, "Ethanol from biomass by enzymatic hydrolysis," Chemical Engineering Progress, vol 84, Aug. 1988.
[13] B. Palmarola-Adrados, P. Choteborska, M. Galbe and G. Zacchi, "Ethanol production from non-starch carbohydrates of wheat bran," Bioresource Technology, vol 96, pp. 843-850, 2005.
[14] T. Srinorakutara, L. Kaewvimol and L.-a. Saengow, "Approach of cassava waste pretreatments for fuel ethanol production in Thailand," The Journal of Scientific Research Chulalongkorn University, vol 31, 2006.
[15] L.G. Miller, "Use of Dinitrosalisalic acid reagent for determination of reducing sugar," Analytical Chemistry, vol 31, Mar. 1959.
[16] K. Yoonan and J. Kongkiattikajorn, "A study of optimal condition for reducing sugars production from cassava peels hydrolysis by dilute acid and enzymes," Kasetsart Journal, 2005
[17] K. Yoonan, J. Kongkiattikajorn and K. Rattanakanokchai, "Ethanol production from acid hydrolysates of cassava pulps using fermentation by Sacchaomyces cerevisiae," Kasetsart Journal, 2005.
[18] B.-H. Um, M.N. Karim and L.L. Henk, "Effect of sulfuric and phosphoric acid pretreatments on enzymatic hydrolysis of corn stover," Applied Biochemistry and Biotechnology, vol 105-108, pp. 115-125, 2003.