Fermentative Production of Dextran using Food Industry Wastes
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 33122
Fermentative Production of Dextran using Food Industry Wastes

Authors: Marzieh Moosavi-Nasab, Mohsen Gavahian, Ali R. Yousefi, Hamed Askari

Abstract:

Dextran is a D-glucose polymer which is produced by Leuconostoc mesenteroides grown in a sucrose-rich media. The organism was obtained from the Persian Type Culture Collection (PTCC) and was transferred in MRS broth medium at 30°C and pH 6.8 for 24 h. After preparation of inoculums, organisms were inoculated into five liquid fermentation media containing either molasses or cheese whey or different combinations of cheese whey and molasses. After certain fermentation period, the produced dextran was separated and dried. Dextran yield was calculated and significant differences in different media were observed. Furthermore, FT-IR analysis was performed and the results showed that there were no significant differences in the produced dextran structures.

Keywords: Dextran, Leuconostoc mesenteroides, Molasses, Whey

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1334578

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3675

References:


[1] A. Tecante, A. Lopez-Munguia, and A. Garcia-Rejon, "Rheological characterization of dextran-enzymatic synthesis media," J. Appl. Polym.Sci., vol. 31, pp. 2337-2350, 1986.
[2] D. Kim, J. F. Robyt, S. Y. Lee, J. H. Lee, and Y. M. Kim, "Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of Leuconostoc mesenteroides B-512FMCM dextransucrase, " . Carbohydrate Research, vol. 338, pp. 1183-1189, 2003.
[3] H. J. Koepsell, and H. M. Tsuchiya, "Enzymatic synthesis of dextran," J. Bacteriol., vol. 63, pp. 293-295, 1952.
[4] M. Dols, M. Remaud-Simeon, R. Willemot, M. Vignon, and P. Monsan, "Structural characterisation of the maltose acceptor-products synthesized by Leuconostoc mesenteroides NRRL B1299 dextransucrase," Carbohydr. Res., vol. 305, pp. 549-559, 1998.
[5] M. Santos, A. Rodriguesb, and J. A. Teixeira, "Production of dextran and fructose from carob pod extract and cheese whey by Leuconostoc mesenteroides NRRL B512 (f)," Biochemical Engineering J., vol. 25, pp. 1-6, 2005.
[6] M. Santos, J. Teixeira, and A. Rodrigues, "Production of dextransucrase, dextran and fructose from sucrose using Leuconostoc mesenteroides NRRL B512 (f)," Biochemical Engineering J., vol. 4, pp. 177-188, 2000.
[7] S. M. Holt, H. Al-Sheikh, and K. J. Shin, "Characterization of dextranproducing Leuconostoc strains," Letters in Applied Microbiology, vol. 32, pp. 185-189, 2001.
[8] V. B. Veljkovic, M. L. Lazic, D. J. Rutic, S.M. Jovanovic, and D.U. Skala, "Effects of aeration on extracellular dextransucrase production by Leuconostoc mesenteroides," Enzyme Microb. Technol., vol. 14, pp. 665-668, 1992.