Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 31097
Effect of Temperature and Time on Dilute Acid Pretreatment of Corn Cobs

Authors: Sirikarn Satimanont, Apanee Luengnaruemitchai, Sujitra Wongkasemjit


Lignocellulosic materials are new targeted source to produce second generation biofuels like biobutanol. However, this process is significantly resisted by the native structure of biomass. Therefore, pretreatment process is always essential to remove hemicelluloses and lignin prior to the enzymatic hydrolysis. The goals of pretreatment are removing hemicelluloses and lignin, increasing biomass porosity, and increasing the enzyme accessibility. The main goal of this research is to study the important variables such as pretreatment temperature and time, which can give the highest total sugar yield in pretreatment step by using dilute phosphoric acid. After pretreatment, the highest total sugar yield of 13.61 g/L was obtained under an optimal condition at 140°C for 10 min of pretreatment time by using 1.75% (w/w) H3PO4 and at 15:1 liquid to solid ratio. The total sugar yield of two-stage process (pretreatment+enzymatic hydrolysis) of 27.38 g/L was obtained.

Keywords: Pretreatment, phosphoric acid, corn cobs, Butanol production

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2433


[1] Icoz, E., Turgrul, K.M.M., Saral, A.M., and Icoz, E. (2009).Research on ethanol production and use from sugar beet Turkey. Biomass and Bioenergy, 33, 1-7.
[2] Palmqvist, E. and Hahn-Hagerdal, B. (2002). Fermentation of lignocellulosic hydrolysates. : inhibitors and mechanisms of inhibition. Bioresource Technology, 74(1), 25-33.
[3] Zheng, Y., Pan, Z.L., and Zhang, R.H. (2009). Overview of biomass pretreatment for cellulosic ethanol production. Agricultural and Biological Engineering, 2,51-68.
[4] Hendriks, A.T.W.M., and Zeeman, G. (2009). Pretreatment to enhance the digestion digestibility of lignocellulosic biomass.Bioresource Technology, 100, 10-18.
[5] Gamez, S.R., Gonzalez-Cabriales, J.J., Ramirez, J.A.B.T.,Garrote, G., Vazquez, M.N. (2006). Study of the hydrolysis of sugar cane bagasses using phosphoric acid. Journal of Food Engineering, 74, 78-88.
[6] Gamez, S., Ramirez, J.A., Garrote, G., Vazquez, M. (2004). Manufacture of Fermentable Sugar Solutions from Sugar Cane Bagasse Hydrolyzed with Phosphoric Acid at atmospheric Pressure, 74, 4172- 4177.
[7] Kumar, P.V., Barrett, D.M., Delwiche, M.C., Stroeve, P.T. (2009). Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuels production. Industrial and Engineering Chemistry Research, 48, 3713-3729.
[8] Ezeji, T., Qureshi, N., and Blaschek, H.P. (2007). Butanol Production From Agricultural Residues: Impact of Degradation Products on Clostridium beijerinckii Growth and Butanol Fermentation. Biotechnology ans Bioengineering, 97, 1460-1469.
[9] Vazquez, M., Oliva, M., Tellez-Luis, S.J. and Ramirez, J.A. (2007). Hydrolysis of sorghum straw using phosphoric acid:Evaluation of furfural production. Bioresource Technology, 98, 3053-3060.
[10] Weil, J R., Dien, B., Bothast, R., Hendrickson, R., Mosier, N.T. S., and Ladisch M.M. R. (2002). Removal of Fermentation Inhibitors Formed during Pretreatment of Biomass by Polymeric Adsorbents. Industry and Engineering Chemistry Research, 41, 6132-6138.
[11] Redding, A.T., Wang, Z.Y., Keshwani, D.P., Cheng, J. (2011). High temperature dilute acid pretreatment of coastal Bermuda grass for enzymatic hydrolysis. Bioresource Technology, 102, 1415-1424.
[12] Almeida, J., Bertilsson, M., Gorwa-Grauslund, M., Gorsich, S., Gunnar, L., (2009). Metabolic effects of fraldehydes and impacts on biotechnological processes. Applied Micribiology and. Biotechnology. 82, 625-638.
[13] Delgenes, J., Moletta, R., Navarro, J., (1996). Effect of lignocellulosic degradation products on ethanol fermentation of glucose and xylose by Saccharomycescerevisiae, Zymomonas mobilis, Pichia stipitis, and Candida shehatae. Enzyme and Microbial Technology, 19, 220-225.
[14] Taherzadeh, M., Eklund, R., Gustafsson, L., Niklasson, C., Liden, G., (1997). Characterization and fermentation of dilute acid hydrolyzates from wood. Industrial and Engineering Chemistry Research, 36, 4659- 4665.