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Anti-microbial Activity of Aristolochic Acid from Root of Aristolochia bracteata Retz
Abstract:The present research was designed to investigate the anti-microbial activity of aristolochic acid from the root of Aristolochia bracteata. From the methanolic & ethyl extract extracts of Aristolochia bracteata aristolochic acid I was isolated and conformed through IR, NMR & MS. The percentage purity of aristolochic acid I was determined by UV & HPLC method. Antibacterial activity of extracts of Aristolochia bracteata and the isolated compound was determined by disc diffusion method. The results reveled that the isolated aristolochic acid from methanolic extract was more pure than the compound from ethyl acetate extract. The various extracts (500μg/disc) of Aristolochia bracteata showed moderate antibacterial activity with the average zone of inhibition of 7-18 mm by disc diffusion method. Among the extracts, ethyl acetate & methanol extracts were shown good anti-microbial activity and the growth of E.coli (18 mm) was strongly inhibited. Microbial assay of isolated compound (Aristolochic acid I) from ethyl acetate & methanol extracts were shown good antimicrobial activity and the zone of inhibition of both at higher concentration 50 μg/ml was similar with the standard aristolochic acid. It may be concluded that the isolated compound of aristolochic acid I has good anti-bacterial activity.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1329064Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1815
 G. Dulger, and C. Aki, "Antimicrobial Activity of the Leaves of Endemic Stachys pseudopinardii in Turkey," Trop J Pharmaceutical Res., 2009, Vol. 8(4), pp. 371-375.
 M. Stuffness, and J. Douros, "Current status of the NCI plant and animal product program," J Nat Prod., 1982, Vol. 45, pp. 1-14.
 J.T. Baker, R.P. Borris, and B. Carte, "Natural product drug discovery and development: New perspective on international collaboration," J Nat Prod., 1995, Vol. 58, pp. 1325-1327.
 F.A. Ripa, M. Haque, and M.I. Haque, "In Vitro Antimicrobial, Cytotoxic and Antioxidant Activity of Flower Extract of Saccharum spontaneum Linn," Euro J Sci Res., 2009, Vol. 30(3), pp. 478-483
 D.A. Ates, and O.T. Erdourul, "Antimicrobial Activities of Various Medicinal and Commercial Plant Extracts," Turk J Biol., 2009, Vol. 27, pp. 157-162
 K.R. Kirtikar, and B.D. Basu, "Indian Medicinal Plants," International Book Distributors, Dehradun, 1935, Vol. I, pp. 139.
 J.L. Rios, M.C. Recio, and A. Villar, "Screening methods for natural products with antimicrobial activity: A review of the literature," J Ethnopharmacol., 1988, Vol. 23, pp. 127- 149.
 J. Hinou, C. Demetzos, C. Harvala and C. Roussakis, "Cytotoxic and Antimicrobial Principles from the Roots of Aristolochia longa," J Ethanopharmacol., 1990, Vol. 28(2), pp. 149-151.
 T.S. Wu, Y.L. Leu, and Y.Y. Chan, "Constituents from the stem and root of Aristolochia kaempferi," Biol Pharm Bull., 2000, Vol. 23(10), pp. 1216-1219.
 T.S. Wu, Y.Y. Chan, Y.L. Leu and Z.T. Chen, "Sesquiterpene esters of aristolochic acid from the root and stem of Aristolochia heterophylla," J Nat Prod., 1999, Vol. 62(3), pp. 415-418.
 P.S. Negi, C. Anandharamakrishnan, and G.K. Jayaprakasha, "Antibacterial activity of Aristolochia bracteata root extracts," J Med Food., 2003, Vol. 6(4), pp. 401-403.
 C.A. Gadhi, M. Weber, F. Mory, A. Benharref, C. Lion, M. Jana, and A. Lozniewski, "Antibacterial activity of Aristolochia paucinervis Pomel," J Ethnopharmacol., 1999, Vol. 67(1), pp. 87-92.
 D. Kavitha, and R. Nirmaladevi, "Assessment of Aristolochia bracteolata leaf extracts for its biotherapeutic potential," Afr J Biotec., 2009, Vol. 8(17), pp. 4242-4244.
 R.V. Manikandar, P. Selvamani, and S. Latha, "Antibacterial activity of leaf extracts of Aristolochia bracteata Retz," Indian J Pharm Sci., 2006, Vol. 68(4), pp. 509-510.