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Production of Glucose from the Hydrolysis of Cassava Residue using Bacteria Isolates from Thai Higher Termites

Authors: Pitcha Wongskeo, Pramoch Rangsunvigit, Sumaeth Chavadej


The possibility of using cassava residue containing 49.66% starch, 21.47% cellulose, 12.97% hemicellulose, and 21.86% lignin as a raw material to produce glucose using enzymatic hydrolysis was investigated. In the experiment, each reactor contained the cassava residue, bacteria cells, and production medium. The effects of particles size (40 mesh and 60 mesh) and strains of bacteria (A002 and M015) isolated from Thai higher termites, Microcerotermes sp., on the glucose concentration at 37°C were focused. High performance liquid chromatography (HPLC) with a refractive index detector was used to determine the quantity of glucose. The maximum glucose concentration obtained at 37°C using strain A002 and 60 mesh of the cassava residue was 1.51 g/L at 10 h.

Keywords: cassava, Glucose, Hydrolysis, termites

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[1] Chang, K.L., Thitikorn-amorn, J., Hsieh, J.F., Ou, B.M., Chen, S.H., Ratanakhanokchai, K., Huang, P.J., and Chen, S.T. (2011). Enhanced enzymatic conversion with freeze pretreatment of rice straw. Biomass and Biology, 35(1), 90 - 95.
[2] Gonzalez, J.F., Rayo, M.C., Roman, S., Gonzalez-Garcia, C.M. and Ledesma, B. (2010). Modelling non-isothermal degradation of olives solid waste: influence of variables and kinetics, third international symposium on energy from biomass and waste. CISA, Environmental Sanitary Engineering Centre, Italy
[3] Mussatto, S.I. and Teixeira, J.A. (2010). Lignocellulose as raw material in fermentation processes. Applied Microbiology and Microbial Biotechnology, 897 - 907.
[4] Pandey, A., Soccol, C.R., Nigam, P., Soccol, V.T., Vandenberghe, L.P.S. and Mohan, R. (2000). Biotechnological potential of agroindustrial residues. II: cassava bagasse. Bioresources Technology, 74(1), 81 - 87.
[5] Nair, M.P.D., Padmaja, G. and Moorthy, S.N. (2011). Biodegradation of cassava starch factory residue and using a combination of cellulases, xylanases and hemicellulases. Biomass and Bioenergy, 35(1), 1211 - 1218.
[6] Sun, Y. and Cheng, J. (2002). Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresource Technology, 83(1), 1 - 11.
[7] Soares, I.B., Travassos, J.A., Baudel, H.M., Benachour, M. and Abreu, C.A.M. (2011). Effects of washing, milling and loading enzymes on the enzymatic hydrolysis of a steam pretreated sugarcane bagasse. Industrial Crops and Products, 33(3), 670 - 675.
[8] Taechapoempol, K., Sreethawong, T., Rangsunvigit, P., Namprohm, W., Thamprajamchit, B., Rengpipat, S., Chavadej, S. (2010). Cellulase- Producing Bacteria from Thai Higher Termites, Microcerotermes sp.: Enzymatic Activities and Ionic Liquid Tolerance. Biochem Biotechnology, 164, 204-219.
[9] Eourarekullart, W. (2011). Conversion of corncob to sugars by microbial hydrolysis. M.S. Thesis, The Petroleum and Petrochemical College, Chulalongkorn University.
[10] Yeh, A., Huang, Y., and Chen, S.H. (2010) Effect of particle size on the rate of enzymatic hydrolysis of cellulose. Carbohydrate Polymers, 79(1), 192-199.