Adherence of Alveolar Fibroblasts and Microorganisms on Titanium Implants

Authors: J. Franková, V. Pivodová, F. Růžička, J. Ulrichová

Abstract:

An implant elicits a biological response in the surrounding tissue which determines the acceptance and long-term function of the implant. Dental implants have become one of the main therapy methods in clinic after teeth lose. A successful implant is in contact with bone and soft tissue represent by fibroblasts. In our study we focused on the interaction between six different chemically and physically modified titanium implants (Tis-MALP, Tis-O, Tis- OA, Tis-OPAAE, Tis-OZ, Tis-OPAE) with alveolar fibroblasts as well as with five type of microorganisms (S. epidermis, S.mutans, S. gordonii, S. intermedius, C.albicans). The analysis of microorganism adhesion was determined by CFU (colony forming unite) and biofilm formation. The presence of α3β1 and vinculin expression on alveolar fibroblasts was demonstrated using phospho specific cell based ELISA (PACE). Alveolar fibroblasts have the highest expression of these proteins on Tis-OPAAE and Tis-OPAE. It corresponds with results from bacterial adhesion and biofilm formation and it was related to the lowest production of collagen I by alveolar fibroblasts on Tis-OPAAE titanium disc.

Keywords: titanium disc, alveolar fibroblasts, microorganisms, adhesion

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1333784

References:

[1] B. H. Zhao, W. M. Tian, H .L. Feng, I. S. Lee and F. Z. Cui, "Current effects of RGD peptide grafting to titanium dental implants on the adhesion of human gingival fibroblasts and epithelial cells," in Appl Phys, 2005, vol.5, pp.407-410.
[2] B. Lia, W.Sunb and Y. Jic, "The effect of Nifedipine on the expression of type I collagen in gingival fibroblasts," JNMU, 2008, vol.22, pp.92- 95.
[3] A. Surazynskia, P. Sienkiewicza, S. Wolczynskib and J. Palka, "Differential effect of echistatin and trombin on collagen production and prolidase activity in human dermal fibroblasts and their possible implication in 1-integrin-mediated signaling," Pharmacol Res, 2005, vol.51, pp.217-221.
[4] P.R. Kramera, A.J: Keitha, Z. Caib, S. Maa and I. Watanabeb, "Integrin mediated attachment of periodontal ligament to titanium surfaces," Dent Mat, 2009, vol.25, pp.877-883.
[5] L. Montanaroa, D. Campocciaa, S Rizzia, M. E. Donatia, L. Breschic, C. Pratic and C.R. Arciolaa, "Evaluation of bacterial adhesion of Streptococus mutans on dental restorative material," Biomaterials, 2004, vol.25, pp.4457-4463.
[6] A. Jabbour, M. Srebnik, B. Zaks, V. Dembitsky and D. Steinberg, "Evaluation of oxazaborolidine activity on streptococcus mutans biofilm formation," Int J Antimicrob Agents, 2005, vol.26, pp.491-496.