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Effects of a Nectandra Membranacea Extract on Labeling of Blood Constituents with Technetium-99m and on the Morphology of Red Blood Cells

Authors: Silvana R.F. Moreno, Jorge J. Carvalho, Ana L. Nascimento, Mario Pereira, Luiz Q. A. Caldas, Mário Bernardo-Filho


The aim of this in vitro study was to evaluate the possible interference of a Nectandra membranacea extract (i) on the labeling of blood cells (BC), (ii) on the labeling process of BC and plasma (P) proteins with technetium-99m (Tc-99m) and (iii) on the morphology of red blood cells (RBC). Blood samples were incubated with a Nectandra membranacea crude extract, stannous chloride, Tc- 99m (sodium pertechnetate) was added, and soluble (SF) and insoluble (IF) fractions were isolated. Morphometry studies were performed with blood samples incubated with Nectandra membranacea extract. The results show that the Nectandra membranacea extract does not promote significant alteration of the labeling of BC, IF-P and IF-BC. The Nectandra membranacea extract was able to alter the erythrocyte membrane morphology, but these morphological changes were not capable to interfere on the labeling of blood constituents with Tc-99m.

Keywords: in vitro study, Nectandra membranacea, red bloodcell, technetium-99m

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[1] Klassen. In Casarett, Doull-s, “Toxicology: The Basis Science of Poisons". 6aEdition. New York: In Macmillan Publishing Company; 2001, pp. 159-171.
[2] M. Rotblat, I. Ziment. “Evidence-Based Herbal Medicine". Hanley & Belfus, Philadelphia Inc. Medical Publishers; 2002, pp. 39-44.
[3] C.C. Moura. “Estudo fitoqu├¡mico de Nectandra membranacea (Spreng.)" Hassler. Qu├¡mica Org├ónica,
[Master-s Thesis] Rio de Janeiro Brazil, UFRJ; 1997, pp. 15-16.
[4] H. Werff, D. Wan. “ A key to the genera of Lauraceous in the world". Ann Missouri Bot Gard, 1991, Vol. 78, 77-387.
[5] P.W. Le Quesne, J.E. Larrahondo, R.F. Raffaul, Antitumor plants X Constituents of Nectandra rigida", J Nat Prod, 1980, Vol. 43, 353- 359.
[6] A. A. Silva-Filho, M. L. Andrade e Silva, J. C.T Carvalho, J. K. Bastos. “Evaluation of analgesic and anti-inflammatory activities of Nectandra megapotamica (Lauracea) in mice and rats", J Pharm Pharmacol, 2004, Vol. 56, p.1179.
[7] H. Fenglin, L. Ruili, B. Huang, M. Liang. “Free radical scavenging activity of extracts prepared from fresh leaves of selected chinese medicinal plants". Fitoterapia 2004, Vol. 75, pp.14-23.
[8] Sampson C. B. “Complications and difficulties in radiolabeling blood cells: a Review", Nuc Med Commun, 1996, Vol. 17, pp.648-658.
[9] H Sinzinger, M Rodrigues. “Cellular labeling with Tc-99m chelates: Relevance of in vitro and in vivo viability testing". In: Zolle I (ed.) Technetium-99m pharmaceuticals: Preparation and quality control in nuclear medicine. Springer Berlin Heidelberg, 2007, pp. 208- 219.
[10] P.J .Early, D B Sodee. Radiopharmaceuticals. In Principles and practice of Nuclear Medicine 2 ed. Missouri: Mosby Year Book, Inc. Toronto, 1996, pp. 89-101.
[11] J. N. Frydman , A.S. Fonseca, V.C. Rocha, M.O. Benarroz, G.S. Rocha, M.O. Pereira, M.J. Pereira, A.C. Medeiros, M Bernardo-Filho. “Acetylsalicylic Acid and Morphology of Red Blood Cells", Braz Arch Biol Technol, 2010, Vol. 53, pp. 575-582.
[12] S.R.F. Moreno, J.J. Carvalho, A.L. Nascimento, M Pereira, E.K. Rocha, G. Dire, M. Bernardo-Filho. “Bioavailability of the sodium pertechnetate and morphometry of isolated from rats: Study of possible pharmacokinetic interactions of a Ginkgo biloba extract", Braz Arch Biol Technol, 2005, Vol. 48, pp. 73-78.
[13] M.L. Gomes, M.N. Oliveira, M. Bernardo-Filho. Drug interaction with radiopharmaceuticals: Effect on the labeling of red blood cells with technetium-99m and on the bioavailability of radiopharmaceuticals. Braz Arch Biol Technol, 2002, 45, pp. 143-149.
[14] S.R.F. Moreno, J.J. Carvalho, B. Rebello, E.K. Rocha, M. Oliveira- Tim├│teo, B. Olej, M. Bernardo-Filho, LQA Caldas. “Experimental Models using Radionuclides for assessment effects of natural drugs", Braz Arch Biol Technol, 2008, Vol. 51, pp. 169-174.
[15] M.M.O. Benarroz, G.S. Rocha, M. Pereira, M. Geller, A.S. Fonseca, G.A. Presta, M. Bernardo-Filho, “Effects of Cinnamomum zeylanicum treatment on radiolabeling of blood constituents, and Morphology of Red Blood Cell in Wistar rats", Braz Arch Biol Technol, 2008, Vol. 51, 143-149.
[16] G. F. Diré, E. Lima, M.L. Gomes, S. Moreno, M.V.C. Faria, R.L. Jales, M.B. Bernardo-Filho. “Evaluation of the Biological effects of a natural extract of chayotte (Sechium edule): A molecular and cellular analysis". Pak J Nut, 2003, Vol. 2, pp. 249-253.
[17] E.A. Lima, G. Diré, D. Mattos , M.L. Gomes , M.B. Oliveira, M.V.C. Faria, M.B. Bernardo-Filho. “Effect of an extract of cauliflower (leaf) on the labeling of blood elements with technetium-99m and on the survival of Escherichia coli AB1157 submitted to the treatment with stannous chloride". Food Chem Toxicol, 2002, Vol. 40, pp. 919-923.
[18] S.R.F. Moreno, R.S. Freitas, E.K. Rocha, G.L. Lima-Filho, M Bernardo- Filho. “Protection of plasmid DNA by Ginkgo biloba from the effects of stannous chloride and the action on the labeling of blood elements with technetium-99m", Braz J Med Biol Res, 2004, Vol, 37: pp. 267-271.
[19] S.R.F. Moreno, E.K. Rocha, M. Pereira, R.S. Freitas, A.L. Nascimento, J.J. Carvalho, M. Bernardo-Filho. “Ginkgo biloba extract: Experimental model to evaluate its action on the labeling of blood elements with technetium-99m and on the morphometry of red blood cells", Pak J Nutr, 2004, Vol. 3, pp. 68-71.
[20] S.R.F. Moreno, A. Arnobio, J.J. Carvalho, A.L. Nascimento, M. Oliveira Tim├│teo, B. Olej, L.Q.A. Caldas, M.B. Bernardo-Filho. “The ingestion of a Nectandra membranacea extract changes the bioavailability of tecnetium-99m radiobiocomplex in rat organs", Biol Res, 2007a, Vol. 40, pp. 131-135.
[21] A.R. Giles, “Guidelines for the use of animals in biomedical research", Thromb Haemost, 1987, Vol. 58, pp. 1078-1984.
[22] J. J. Harbert. “Production of radionuclide: In JL Harbert, WC Echelman, RD Newman, Nuclear medicine. Diagnosis and therapy". Thieme Medical Publishers. Inc, New York, 1996.
[23] L. Junqueira & J. Carneiro, “Histologia B├ísica" 11┬¬ edi├º├úo. Guanabara Koogan, Rio de Janeiro, Brasil, 2007.
[24] S.R.F. Moreno, A. Arnobio, J.J. Carvalho, A.L. Nascimento, M. Oliveira Tim├│teo, B. Olej, L.Q.A. Caldas, “Effect of oral ingestion of an extract of the herb Uncaria tomentosa on the biodistribution of sodium pertechnetate in rats". Braz. J Med Biol Res Biol, 2007b, Vol. 40, pp. 77-80.
[25] G.B. Saha, “Fundamentals of Nuclear Pharmacy". 6 Ed. New York: Springer-Verlag, 2006.
[26] J.F. Oliveira, A.C. Braga, A.S. ├üvila, L.B. Fonseca, B. Gutfilen, M. Bernardo-Filho, “Effect of Thuya occidentalis on the labeling of red blood cells and plasma proteins with technetium-99m". Yale J Biol Med, 1997, Vol. 69, pp. 489-494.
[27] J.F. Oliveira, A.S.├üvila, A.C. Braga, M.B.N. Oliveira, E.M. Boasquevisque, R.L. Jales, M. Bernardo-Filho, “Effect of extract of medicinal plants on the labeling of blood elements with technetium-99m and on the morphology of red blood cells: I - a study with Paullinia cupana", Fitoterapia 2002, Vol. 73, pp. 305-312.
[28] J.F. Oliveira, M.B. Oliveira, A. Avila, A.C.S. Braga, M.T.J.A. Catanho, R.L.C. Jales, M. Bernardo-Filho, “Assessment of the effect of Fucus vesiculosus extract on the labeling of blood constituents with technetium-99m and the histological modifications on the shape of the red blood cells". Food Chem Toxicol, 2003, Vol 41, pp. 15-20.
[29] J.F. Oliveira-Fernandes, L.C. Brito, J.N.G. Frydman., S. Santos-Filho, M. Bernardo-Filho, “An aqueous extract of Pfaffia sp. does not alter the labeling of blood constituents with technetium-99m and morphology of the red blood cells", Braz J Pharmacogn, 2005, Vol 15, pp. 126-132.
[30] A.L. Dantas, L.M.M.V. Valente, L. A. S. Moraes, G. Feliciano and M. Bernardo-Filho, “In vitro study of Vellozia pusilla Pohl (Velloziaceae) brazilian species of plant: Antitumoral activity and labeling of blood elements". Braz Arch Biol Technol, 2005, Vol 48, pp. 63-68.
[31] A.S. Fonseca, J.N.G. Frydman, V.C. Rocha, M. Bernardo-Filho, “Acetylsalicylic acid and labeling of blood constituents with technetium- 99m", Braz Arch Biol Technol, 2005, Vol. 48, pp. 163-168.
[32] S. Hesslewood, E. Leungeung, “Drug interactions with radiopharmaceuticals". Eur J Nucl Med, 1994, Vol 21, pp. 348-356.
[33] J. Kawabe, S. Higashiyama, K. Torii, T. Okamura, J. Kotani, E. Kawamra, “Poor labeling of Tc-99m red blood cells in vivo in a radionuclide intestinal bleeding study of a patient who had recently undergone frequent blood transfusions". Clin Nuc Med, 2003, Vol. 28, pp. 911-912.
[34] P. G. Pietta, “Flavonoids as antioxidants", J Nat Prod, 2000, Vol 63, pp. 1035-1042.
[35] J.F. Oliveira, S.D. Santos Filho, M.T.J. Catanho, S.C. Srivastava, G. Lima Filho, M. Bernardo Filho, “Effect of extract of medicinal plant on the labeling of blood elements with technetium-99m and on the morphology of red blood cells (RBC): Toxicology action of roast coffee beans (Coffea arabica)". Indian J Nucl Med, 2003a, Vol 18, pp. 52-56.
[36] S.D. Santos-Filho, G.L. Diré, E. Lima, M.N. Oliveira, M. Bernardo Filho, “Effect of Mentha crispa (mint) extract on the labeling of blood elements with technetium-99m: a possible evaluation of free radicals", J Biol Sci, 2004, Vol. 4, pp. 266-270.
[37] B. Gutfilen, E.M. Boasquevisques, M. Bernardo-Filho, “Cacium chanel blockers: Interference on red blood cells and plasma proteins labeling with 99m Tc", Rev Esp Med Nuclear, 1992, Vol. 11, pp. 195-199.
[38] I.B. Bakaltheva, C.O. Odeyale, B. J. Spargo, “Effects of alkanols, alkanediols and glycerol on red blood cell shape and hemolysis", Biochem Bophis Acta, 1996, Vol. 1280, pp. 73-80.
[39] M.V. Freitas, R.C. Neto, J.C.C. Huss, T.M.T. Souza, J.O. Costa, C.B. Firmino, M. Bernardo-Filho, “Influence of aqueous crude extracts of medicinal plants on the osmotic stability of human erythrocytes", Toxicol in vitro, 2008, Vol. 22, pp. 219-224.
[40] C.C. Cunha, L.R. Arvelos, J.O. Costa, N. Penha-Silva, “Effects of glycerol on the thermal dependence of the stability of human erytrocytes", J Bioenergy Bioembr, 2007, Vol 39, pp. 341-347.
[41] E. G. Gros, A. Pomillo, A. M. Seldes, G. Burton, “ Introduccion al studio de los produtos naturals: Monografia n. 30", Eva Jota (ed) Secretaria General de la Organization de los Estados Americanos, Inc. Washington, 1985, pp. 62-63.