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Antinociceptive and Anti-inflammatory Effects of Hydroalcohol Extract of Vitex agnus castus Fruit
Abstract:In present study the effects of anti-inflammatory and antinociceptive of vitex hydro-alcoholic extract were evaluated on male mice. In inflammatory test mice were divided into 7 groups: first group was control. The second group, positive control group, received dexamethasone (15 mg/kg) and the other five groups received different doses of hydroalcohol extract of Vitex fruit (265, 365, 465, 565, and 665 mg/kg). The inflammation was caused by xylene-induced ear edema. Formalin test was used for evaluation of antinociceptive effect of extract. In this test, mice were divided into 7 groups: control, morphine (10mg/kg) as positive control group, and Vitex extract groups ((265, 365, 465, 565, and 665 mg/kg). All drugs were administered intrapritoneally, 30 min before each test. The data were analyzed using one-way ANOVA followed by Tukey-kramer multiple comparison test. Results have shown significant antiinflammatory effects of extract at all dosed as compared with control (P<0.05). In the first phase of formalin test (0-5 min) none doses of extract could inhibit acute pain, but in the second phase (15-30 min) chronic pain decreased at 265, 365, 465, and 565 mg/kg doses (P<0.05). The results of this research indicated that Vitex extract remarkably inhibited inflammation and second phase of nociception (inflammatory pain) and can be used for treatment of inflammatory diseases.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1332992Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1854
 A. Zargari, Medical plants. Tehran: Tehran Univ Pub: 1996, pp. 716-718.
 B. Roemheld-Hamm, "Chasteberry", Am Fam Physician. , vol 72, no 5, pp. 821-824, 2005.
 C. Daniele, J .Thompson Coon, MH. Pittler and E. Ernst, "Vitex agnus castus :a systematic review of adverse events", Drug Saf ., vol 28, no 4, pp. 319-332, 2005.
 JJ. Dugoua, D. Seely, D. perri, G. Koren and E. Mills, "Safety and efficacy of chastetree (Vitex agnus castus) during pregnancy and lactation", Can J Pharmacol., vol 15, no 1, pp. 74-79, january 2008.
 K. Ohyama, "Cytotoxicity and apoptotic inducibility of Vitex agnuscastus fruit extract in cultured human normal and cancer cells and effect on growth", Biol Pharm Bull., vol 26, no 1, pp. 10-18, Oct. 2003.
 S. Arokiyaraj, K. perinbam, P. Agastian and RM. Kumar, "Phytochemical analysis and antibacterial activity of Vitex agnus castus", Int J Green Pharm., vol 3, pp. 162-164, Aug. 2009.
 M. Azarnia, S. Ejtemaei-Mehr, A. Shakoor and A. Ansari, "Effects of Vitex agnus castus on mice fetus development", Acta Medica Iranica, vol 45, no 4:pp 263-270, Feb. 2007.
 MG. Dharmasiri, JR. Javakody, G. Galhena, SS. Livanage and WD. Ratnasoorya. "Anti-,inflammatory and analgesic activities of mature fresh leaves of Vitex negundo", J Ethnopharm., vol 87, pp. 199-206, Apr. 2003.
 Y. Hu, HL. xin, QY. Zhang, HC. Zheng, K. Rahman and LP. Qin, "Antinociceptive and anti-hyperprolactinemia activities of fructus victies and its effective fractions and chemical constituents". Phytomedicine., vol 14, no 10, pp. 668-674, Mar. 2007.
 E. Nyiligira, AM. Viljoen, FR. Van Heerden, RL. Van Zyl, S F. Van Vuuren, and PA. Steenkamp, "Phytochemistry and in vitro pharmacological activities of South African Vitex (Verbenaceae) species". Ethnopharm., vol 119, no 3, pp. 680-685, July. 2008.
 S. Nasri and S. Ebrahimi, " Medical effect of Vitex agnus- castus". Journal of Babol University of medical Science., vol 7, pp. 49-53, 2006.
 S, Nasri, SH. Oryan, AH. Rohani and GHR. Amin, "The effect of Vitex agnus castus extract on LH and testostrone in male mice", Pakistan J of Biol Sci., vol 10, no14, pp. 2300-2307, 2007.
 AH. Atta and A. Alkofahi, "Anti-nociceptive and anti-inflammatory effects of some Jordanian medical plant extracts". J of Ethnopharm., vol 60,pp. 117-124, Nov. 1998.
 S. Hunskaar and K. Hole, "The formalin test in mice:dissociation between inflammatory and non-inflammatory pain", Pain, vol 30, pp. 103-114, 1987.
 M. Ramezani, S. Nasri and N. Yasa, "Antinociceptive and antiinflammatory effects of isolated fractions from Apium graveolens seeds in mice". Pharmaceutical Biol, vol 47, no 8, pp. 740-743, Oct. 2009.
 A. Tjolsen, DG. Berge, S. Hunskaar, JH. Rosland and K. Hole, "The formalin test: an evaluation of the method". Pain, vol 51, pp. 5-17, June. 1992.
 D. Cossuta, E. Simndi, J. Vagi, A. Hohmann and I. Prech, "Supercritical fluid extraction of Vitex agnus castus fruit", J of Supercritical Fluids., Vol 47, pp. 188-194, July. 2008.
 J.R. Kuiatc, P.H. Amvam Zollo, G. Lamaty, C. Mentu, j.M. Bessciere, "Composition of essential oils from the leaves of two varieties of Aspilia ofricana (pers) Cd Adams from Cameroon". Journal of Flavor and fragrance, vol 14, pp. 167-169, 1999.
 D. Falcao, S. Gomes, N. Aquino, N. Teixeira, M. Arcanjo, D. Vasconcelos and et al. "Antinociceptive effect of the monoterpene R- (+)-limonene in mice". Biol Pharm Bull, vol 30, no 7, pp. 1217- 1220, July. 2007.
 S. Golshani, F. Karamkhani, HR. Monsef-Esfehani and I. Abdollah Mohammad, "Antinociceptive effects of the essential oil of Dracocephalum kotschyi in the mouse writhing test". J Pharm Pharmaceut Sci, vol 7, no 1, pp. 76-79, Apr. 2004.
 S. Lin, H. Zhang, T. Han, JZ Wu, K. Rahman and et al. "In vivo effect of casticin on acute inflammation". Zhang Xi Yi Jie He Xue Bao., vol 5, no 5, pp. 573-576, Sep. 2007.
 MG. Dharmasiri, JR. Javakody, G. Galhena, SS. Livanage and WD. Ratnasoorya, "Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo", J Ethnopharm., vol 87, no 2-3, pp. 199- 206, Aug. 2003.
 MI. Choudhary, Azizuddin, S. Jalil, SA. Nawaz, Km. Khan, RB. Tareen and Rahman. Atta-ur, "Anti-inflammatory and lipoxygenase inhibitory compounds from Vitex agnus-castus". Phytoter Res., vol23, no 9, pp. 1336-1339, sep. 2009.