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Solid State Fermentation of Cassava Peel with Trichoderma viride (ATCC 36316) for Protein Enrichment

Authors: Olufunke O. Ezekiel, Ogugua C. Aworh


Solid state fermentation of cassava peel with emphasis on protein enrichment using Trichoderma viride was evaluated. The effect of five variables: moisture content, pH, particle size (p), nitrogen source and incubation temperature; on the true protein and total sugars of cassava peel was investigated. The optimum fermentation period was established to be 8 days. Total sugars were 5-fold higher at pH 6 relative to pH 4 and 7-fold higher when cassava peels were fermented at 30oC relative to 25oC as well as using ammonium sulfate as the nitrogen source relative to urea or a combination of both. Total sugars ranged between 123.21mg/g at 50% initial moisture content to 374mg/g at 60% and from 190.59mg/g with particle size range of 2.00>p>1.41mm to 310.10mg/g with 4.00>p>3.35mm.True protein ranged from 229.70 mg/g at pH 4 to 284.05 mg/g at pH 6; from 200.87 mg/g with urea as nitrogen source and to 254.50mg/g with ammonium sulfate; from 213.82mg/g at 50% initial moisture content to 254.50mg/g at 60% moisture content, from 205.75mg/g in cassava peel with 5.6>p> 4.75mm to 268.30 in cassava peel with particle size 4.00>p>3.35mm, from 207.57mg/g at 25oC to 254.50mg/g at 30oC Cassava peel with particle size 4.00>p>3.35 mm and initial moisture content of 60% at pH 6.0, 30oC incubation temperature with ammonium sulfate (10g N / kg substrate) was most suitable for protein enrichment with Trichoderma viride. Crude protein increased from 4.21 % in unfermented cassava peel samples to 10.43 % in fermented samples.

Keywords: Cassava peel, Solid state fermentation, Trichoderma viride, Total sugars, True protein.

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[1] D. Grigg. 1995. "The pattern of world protein consumption". Geoforum., 1995, 26 (1): 1-17.
[2] R. Macrae, R.K. Robinson, and M.T. Sadler. Encyclopedia of Food Science, Food Technology and Nutrition. Academic Press Inc., 1993, 4835-4836.
[3] E.A. Iyayi .and D.M. Losel. "Protein Enrichment of cassava By-Products through Solid State Fermentation by Fungi". The Journal of Food Technology in Africa, 2001, 6, 4: 116-118.
[4] B. Nestel. "Current utilization and future potential for cassava". Chronic Cassava Toxicity. Eds. Nestel, B., Macintyre, R. Ottawa: International Development Centre. 1973, 11-26.
[5] D. Subrahmanyan,. "Processing: Fermented Foods of Cassava". Food Laboratory News, 1990, No.21, 6 (3): 9-12.
[6] I.A. Akinrele. "Further studies on the fermentation of cassava". Research. Report No.20 of the Federal Institute of Industrial Research, Oshodi, Nigeria.1967.
[7] A.A. Onilude. "Effect of cassava cultivar, age and pretreatment processes of cellulase and xylanase production from cassava waste by Trichoderma harzanium". Journal of Basic Microbiology, 1996, 36.6: 421-431.
[8] E.A. Iyayi and O.O. Tewe. "Effect of protein deficiency in utilization of cassava peel by growing pigs". Cassava as Livestock feed in Africa. Eds.S.K. Haha, L. Reynolds and G.N. Egbunike.Proceedings of the IITA/ILCA/University of Ibadan workshop.14-18 November, 1988, pp54-59.
[9] A. M. Almazan. "Cyanide and its reduction in cassava processing into some products". Cassava Processing Utilization Workshop. Southern Africa Development. Co-ordination Conference Mzuzu, Malawi. 7-11, 1998.
[10] O.A. Ubalua. "Cassava wastes: treatment options and value addition alternatives". African Journal of Biotechnology, 2007, 6 (18): 2065-2073.
[11] J. Gomez, M. Pazos, S.R. Couto, M.A. "Sanroman. Chestnut shell and barley bran as potential substrates for laccase production by Coriolopsis rigida under solid-state conditions". Journal of Food Engineering, 2005, 68: 315-319.
[12] M. Raimbault. "General and Microbiological aspects of Solid Substrate Fermentation". Electronic Journal of Biotechnology: Process Biotechnology, 1998, 1:3. Accessed 23/03/2006
[13] R.L. Howard, E. Abotsi, E.I. Jansen van Rensburg and S. Howard. "Lignocellulose biotechnology: issues in bioconversion and enzyme Production". African Journal of Biotechnology, 2003, 12: 602-919.
[14] H.E. Grethlein. "Pretreatment for enhanced hydrolysis of cellulosic biomass". Biotechnology Advances 1984, 2: 43-62.
[15] H.E. Grethlein and A.O. Converse. "Common Aspects of acid prehydrolysis and steam explosion for pretreating wood". Bioresource Technology, 1991, 36: 77-82.
[16] J.E. Smith, J.G. Anderson .and E.K. Senior. "Bioprocessing of lignocelluloses". Phil.Trans R. Soc. London Ser. A., 1987, 321: 507-521.
[17] A. L. Kumakura. "Preparation of Immobilized cellulose beads and their Application to hydrolysis of cellulosic materials". Process Biochemistry, 1997, 32:555-559.
[18] Z. Wu and Y.Y. Lee. "Inhibition of the enzymatic hydrolysis of cellulose by ethanol". Biotechnology Letter, 1997, 19:977-979.
[19] E.H. Depaula, L.P. Ramos and M.D. Azevedo. "The Potential of Humicola grisea var. Thermoidea for bioconversion of sugarcane bagasse". Bioresource Technology, 1999, 68:35-41.
[20] L.G. Tang, D.N.S. Hon, S.H. Pan, Y.Q. Zhu, Z. Wang and Z.Z. Wang. "Evaluation of microcrystalline cellulose changes in ultrastructural characteristics during preliminary acid hydrolysis". Journal of Applied Polymer Science, 1996, 59: 483-488.
[21] L.T. Fan, M.M. Gharpuray and Y.H. Lee. Cellulose Hydrolysis. Berlin, Germany: Springer-Verlag, 1987, 3: 1-68.
[22] T.V. Ojumu, B.O. Solomon, E. Betiku, S.K. Layokun and B. Amigun. African Journal of Biotechnology, 2003, 2,6: 150-152.
[23] G.E. Harman. "Trichoderma spp. Including T. harzianium, T. viride, T. koningi, T. .hamatum and other spp. Deutomycete, Monilitates (asexual classification)". Biological control: A guide to Natural enemies in North-america. Weeden, Shelton, Li and Hoffmann, Editors, CornellUnivedrsity.www.nysaes.cornell.edeu/ent/biocontrol/toc.html. ( Accessed 08/06/2006)
[24] K. Kuhls, E. Lieckfeld, T. Börner, and E. Guého. "Molecular reidentification of human pathogenic Trichoderma isolates as T.longibrachiatum and T.citrinoviride". Medical Mycology, 1999, 37.1: 25-33.
[25] A.O.A.C. Official methods of analysis. "Association of Official Analytical Chemists". Washington, DC. 14th edition, 1995.
[26] A.O.A.C. Official methods of analysis. "Association of Official Analytical Chemists". Washington, DC. 13th edition, 1990.
[27] O.H. Lowry, N.J. Rosebrough, A. L. Farr and R. J. Randall. "Protein Measurement with Folin-phenol reagent". Journal of Biological Chemistry, 1951,193: 265-275.
[28] M. Dubois. K.A. Gilles, J. K. Hamilton, P.A. Rebers, and F. Smith. "Colorimetric method for determination of sugars and related substances". Analytical Chemistry, 1956, 28.3: 250-356.
[29] R.D. Cooke. "An enzymatic assay for total cyanide content of cassava (manihot esculanta Crantz)". Journal of Science Food and Agriculture,1978 ,29:345-352.
[30] S. Roussos, M. Raimbault, J.P. Prébois and B.K. Losane 1993. "Zymotis, a large scale solid state fermenter: Design and Evaluation". Applied Biochemistry and Biotechnology 42:37-52.
[31] C.C. Duru and N.U. Uma. "Protein enrichment of solid waste from cocoyam (Xanthosoma sagittifolium(L.)Schott) cormel processing using Aspergillus oryzae obtained from cormel flour". African Journal of Biotechnology 2003, 2(8): 228-232
[32] L. Camacho-Ruiz,N. Perez-Guerra and R.P. Roses. "Factors affecting the growth of Saccharomyces cerevisea in batch culture and in solid state fermentation". Electronic Journal of Environment, Agriculture and Food Chemistry, 2003, 2(5): 531-542.
[33] A. Pandey. "Recent process developments in solid-state fermentation". Process Biochemistry, 1992, 27: 109-117.
[34] P.Gervais and P.Molin. "The role of water in solid-state fermentation". Biochemical Engineering Journal, 2003, 13: 85-101.
[35] C. Balagopalan. "Improving the nutritional value of cassava by Solid state fermentation: CTCRI Experiences". Journal of Scientific and Indusrtial Research, 1996, 5, 479-482.
[36] P. Mathot and J. Brakel J. "Microbial upgrading of feed by solid state fermentation". Mededelingen van de Faculteit Land bouwweschappen, Rijksuniversiteit Gent, 1991, 56: 1611-1618.
[37] C.R. Soccol. "Physiologie et metabolism de Rhizopus en culture solidenet submerge, en relation avec la degradation d- amidon cru et la production d-acide L (+) lactique". PhD thesis Universite Technologique de Compiegne, France, 1992, pp. 219.
[38] A.A. Onilude. "Production, Characterisation and utilization of some dietary fiber degrading enzymes as additives in Broiler diets". Ph.D. Thesis, Department of Botany and Microbiology University of Ibadan, Nigeria.1994.
[39] O.A. Abu. "Biochemical characteristic and utilization of processed sweet potato legume batata(L) LAM for rabbit feeding". Ph.D. Thesis, Univeristy of Ibadan, Ibadan, Nigeria.1997
[40] A. Chesson. Feed enzymes. Animal Feed Science Technology, 1993, 45: 65-79
[41] G. Oboh and A.A. Akindahunsi. Biochemical changes in cassava products (flour and garri) subjected to Saccharomyces cerevisae solid media fermentation. Food Chemistry, 2003, 82(4):559-602.
[42] C. E. Okpako, V.O. Ntui, A.N. Osuagwu and F.I. Obasi. Proximate composition and cyanide content of cassava peels fermented with Aspergillus niger and Lactobacillus rhamnosu. Journal of Food, Agriculture & Environment 2008 6 (2) : 251 - 255.