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Determination of in Vitro Susceptibility of the Typhoid Pathogens to Synergistic Action of Euphorbia Hirta, Euphorbia Heterophylla and Phyllanthus Niruri for Possible Development of Effective Anti-Typhoid Drugs
Abstract:Studies were carried out to determine the in vitro susceptibility of the typhoid pathogens to combined action of Euphorbia hirta, Euphorbia heterophylla and Phyllanthus niruri. Clinical isolates of the typhoid bacilli were subjected to susceptibility testing using agar diffusion technique and the minimum inhibitory concentration (MIC) determined with tube dilution technique. These isolates, when challenged with doses of the extracts from the three medicinal plants showed zones of inhibition as wide as 26±0.2mm, 22±0.1mm and 18±0.0mm respectively. The minimum inhibitory concentration (MIC) revealed organisms inhibited at varying concentrations of extracts: E. hirta (S. typhi 0.250mg/ml, S. paratyphi A 0.125mg/ml, S. paratyphi B 0.185mg/ml and S. paratyphi C 0.225mg/ml), E. heterophylla (S. typhi 0.280mg/ml, S. paratyphi A 0.150mg/ml, S. paratyphi B 0.200mg/ml and S. paratyphi C 0.250mg/ml) and P. niruri (S. typhi 0.150mg/ml, S. paratyphi A 0.100mg/ml, S. paratyphi B 0.115mg/ml and S. paratyphi C 0.125mg/ml). The results of the synergy between the three plants in the ration of 1:1:1 showed very low MICs for the test pathogens as follows S. typhi 0.025mg/ml, S. paratyphi A 0.080mg/ml, S. paratyphi B 0.015mg/ml and S. paratyphi C 0.10mg/ml with the diameter zone of inhibition (DZI) ranging from 35±0.2mm, 28±0.4mm, 20±0.1mm and 32±0.3mm respectively. The secondary metabolites were identified using simple methods and HPLC. Organic components such as anthroquinones, different alkaloids, tannins, 6-ethoxy-1,2,3,4-tetrahydro-2,2,4-trimethyl and steroids were identified. The prevalence of Salmonellae, a deadly infectious disease, is still very high in parts of Nigeria. The synergistic action of these three plants is very high. It is concluded that pharmaceutical companies should take advantage of these findings to develop new anti-typhoid drugs from these plants.
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 Minor L., and Popoff, M.Y (1987). Designation of Salmonella Enterica. (465468). Nea M.I. (2002). Fourth Edition Medical Pharmacology at a glance.
 Health Protection Report (2009). http://www.hpa.org.uk/hpr/archives/2009/news1409.htm reptiles Okoko, T. and Oruambo, I. F. (2008). The effects of Hibiscus sabdariffa calyx on cisplatin-induced tissues damaged in rats. Biokemistri. 20(2):47-52.
 Okoko, T. and Oruambo, I. F. (2008). The effects of Hibiscus sabdariffa calyx on cisplatin-induced tissues damaged in rats. Biokemistri. 20(2):47-52.
 Adebooye, O.C. and Opabode, J.T. (2004). Status of conservation of the indigenous leaf vegetables and fruits of Africa. Afr. J. Biotech. 3:700-705.
 Szeto, Y.P., Tomlinson, B., Benzie, I.F.F. (2002). Total antioxidant and ascorbic acid content of fresh fruits and vegetables: Implications for dietary planning and food preservation. British Journal of Nutrition. 87:55-59.
 Jimoh, F.O., Adebayo, A.A., Aliero, A.A., Afolayan, A.J. (2008). Polyphenol contents and Biological activities of Rumex ecklonianus. Pharm. Biol. 45(5):333-340.
 Buchanan, R. and Gibbons, N.E. (1974). Bergey-s manual of determinative bacteriology. 8th edition,.Baltimore: Williams and Wilkins. 113-136.
 Middle Brooks Pharmaceuticals (2009). http://www.middlebrook pharma.com/NEWSROOM antibiotics/news details.
 Silva, O., Daurk, A., Pimentel, M., Viegas, S., Barroso, H., Machado, J., Pires, I., Carbrita, J. and Gomes, E. (1997). Antimicrobial Activity of Terminilia macroptera root. Journal of Ethnopharmacology. 57:203-207.
 Abalaka, M. E., Onaolapo, J.A., Inabo, H.I. and Olonitola, O.S. (2009). Extraction of Active Components of Mormodica Charantia L (Cucurbitaceae) for Medicinal Use. Afr J Biomed Eng & Sc, (1): 38 -44
 Hugo, S. B. and Rusell, A. D. (2003) Pharmaceutical microbiology, 6th edition, Blackwell scientific publishers, Oxford, London Pp. 91-129.
 Sofowora, A., (1993) Medicinal Plant and Traditional Medicine in Africa, 2nd Edition, Spectrum Books Ltd. Ibadan, Nigeria. 50 - 58.
 Trease, G.E. AND Evans, W.C. (1989). A text book of Pharmacognosy, 13th edition, Bailliere Tindall Ltd., London. 394.
 Abalaka, M. E., Daniyan, S. Y and Mann, A (2010). Evaluation of the antimicrobial activities of Two Ziziphus species (Ziziphus mauritiana L. and Ziziphus spinachristi L.) on some microbial pathogens. African Journal of Pharmacy and Pharmacology 4(4),135-139.
 Alade, R.I and Irobi, O.N. (2002). Antimicrobial activities of crude leaf extracts of Acalypha wilkisiana. Journal of Ethnopharmacology. 39:171- 174.
 Martinez, M.J. (2004). Screening of some Cuban Medicinal plants for antimicrobial activity. Journal of Ethnopharmacology. 52:174-178.
 Martos, F.J.A., Aguian, L.M. and Silva, M.G. (2002). Chemical constituents and antimicrobial activities of Vatairea macrocarpa. Ducke Acta Amazonia. 18:351-352.
 Marwan, A.G. (2003). Quantitative determination of infinite inhibition concentration of antimicrobial agents. Applied Environmental Microbiology. 51(3):559-561.
 Wagner, H., Blandts, S.and Zgainki, E.M. (2000). Plant Drug Analysis. Spring-Verlag, New York. 320.