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Effectiveness of Moringa oleifera Coagulant Protein as Natural Coagulant aid in Removal of Turbidity and Bacteria from Turbid Waters

Authors: B. Bina, M.H. Mehdinejad, Gunnel Dalhammer, Guna RajaraoM. Nikaeen, H. Movahedian Attar


Coagulation of water involves the use of coagulating agents to bring the suspended matter in the raw water together for settling and the filtration stage. Present study is aimed to examine the effects of aluminum sulfate as coagulant in conjunction with Moringa Oleifera Coagulant Protein as coagulant aid on turbidity, hardness, and bacteria in turbid water. A conventional jar test apparatus was employed for the tests. The best removal was observed at a pH of 7 to 7.5 for all turbidities. Turbidity removal efficiency was resulted between % 80 to % 99 by Moringa Oleifera Coagulant Protein as coagulant aid. Dosage of coagulant and coagulant aid decreased with increasing turbidity. In addition, Moringa Oleifera Coagulant Protein significantly has reduced the required dosage of primary coagulant. Residual Al+3 in treated water were less than 0.2 mg/l and meets the environmental protection agency guidelines. The results showed that turbidity reduction of % 85.9- % 98 paralleled by a primary Escherichia coli reduction of 1-3 log units (99.2 – 99.97%) was obtained within the first 1 to 2 h of treatment. In conclusions, Moringa Oleifera Coagulant Protein as coagulant aid can be used for drinking water treatment without the risk of organic or nutrient release. We demonstrated that optimal design method is an efficient approach for optimization of coagulation-flocculation process and appropriate for raw water treatment.

Keywords: MOCP, Coagulant aid, turbidity removal, E.coliremoval, water, treatment

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[1] A. Diaz, N. Rincon, A. Escorihuela, N. Fernandez, E. Chacin, and C.F. Forster, A preliminary evaluation of turbidity removal by natural coagulants indigenous to Venezuela. Process Biochemistry, 1999. 35: 391-395.
[2] G.K. Folkard, J. Sutherland,.and R. Shaw, Water clarification using Moringa oleifera seed coagulant . London: Intermediate Technology Publications. 1999. p.109-112.
[3] K.A. Ghebremichael, Moringa Seed and Pumices as Alternative Natural Materials for Drinking Water Treatment. Sweden : Royal Institute of Technology (KTH). Stockholm. PhD. Thesis 2004. p.10-11.
[4] H. Agrawal, C. Shee, and A.K. Sharma, Isolation of a 66 kDa Protein with Coagulation Activity from Seeds of Moringa Oleifera. Research Journal of Agriculture and Biological Sciences. 2007. 3(5): 418-421.
[5] K.A. Ghebremichael, K.R. Gunaratna, H. Henriksson, H, Brumer, and G. Dalhammer, A simple purification and activity assay of the coagulant protein from Moringa oleifera seed. Water Research 2005..39:2338-2344.
[6] H.M. Kwaambwa, and R. Maikoker, A fluorescence spectroscopic study of a coagulating protein extracted from Moringa Oleifera seeds. Colloids and surfaces B: Biointerfaces. 2007. p.1-8.
[7] S.A. Muyibi, and L.M. Evison, Coagulation of turbid water and softening of hard water with Moringa oleifera seeds. Int J Environ Stud. 1996. .56: 483-495.
[8] M. Suarez, M. Haenni, S. Canarelli, F. Fisch, P. Chodanowski, C. Servis, and O. Michielin, Structure-Function Characterization and Optimization of a Plant- Derived Antibacterial Peptide. Antimicrobial Agents and Chemotherapy. 2005.49(9):3847-3857.
[9] APHA, AWWA and WPCF. Standard Methods for the Examination of Water and Wastewater, 21th Edn, Washington DC: American Public Health Association. 2005.
[10] ASTM. Standard Practice for Coagulation-/Flocculation Jar Test of Water E1-1994 R, D 2035-80. Annual Book of ASTM Standards. 1995. 11.02.
[11] EPA. National Primary Drinking Water Standards. EPA. 2003. 816-F- 03-016.