Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30840
Comparative Study on Production of Fructooligosaccharides by p. Simplicissimum Using Immobilized Cells and Conventional Reactor System

Authors: Noraziah A. Y., Mashitah M. D., Subhash Bhatia


Fructooligosaccharides derived from microbial enzyme especially from fungal sources has been received particular attention due to its beneficial effects as prebiotics and mass production. However, fungal fermentation is always cumbersome due to its broth rheology problem that will eventually affect the production of FOS. This study investigated the efficiency of immobilized cell system using rotating fibrous bed bioreactor (RFBB) in producing fructooligosaccharides (FOS). A comparative picture with respect to conventional stirred tank bioreactor (CSTB) and RFBB has been presented. To demonstrate the effect of agitation intensity and aeration rate, a laboratory-scale bioreactor 2.5 L was operated in three phases (high, medium, low) for 48 hours. Agitation speed has a great influence on P. simplicissimum fermentation for FOS production, where the volumetric FOS productivity using RFBB is increased with almost 4 fold compared to the FOS productivity in CSTB that only 0.319 g/L/h. Rate of FOS production increased up to 1.2 fold when immobilized cells system was employed at aeration rate similar to the freely suspended cells at 2.0 vvm.

Keywords: Productivity, Prebiotics, Fructooligosaccharides, immobilized

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1671


[1] J.W. Yun, "Fructooligosaccharides - Occurrence, preparation and application" Enzyme and Microbial Technology, vol 19, pp. 107-117, 1996.
[2] G. Flamm, W. Glinsmann, D. Kritchevsky, L. Prosky, M. Roberfroid, "Inulin and oligofructose as dietary fiber: a review of the evidence" CRC Critical Reviews in Food Science and Nutrition, vol 41, pp. 353-362, 2001.
[3] K.N. Lee, D. Kritchevsky, M.W. Pariza, "Conjugated linoleic acid and atherosclerosis in rabbits" Atherosclerosis, vol 108, pp. 19-25, 1994.
[4] S.F. Chin, W. Liu, J.M. Storkson, Y.L. Ha, M.W. Pariza, "Dietary sources of conjugated dienoic isomers of linoleic acids, a newly recognized class of anticarcinogens" Journal of Food Composition and Analysis, vol 5, pp. 185-197, 1992.
[5] R.J. Henry, B. Darbyshire, "Sucrose fructosyltransferase and fructan fructosyltransferase from Alium cepa" Phytochemistry, vol 19, pp. 1017- 1020, 1980.
[6] A. Nemukula, T. Mutanda, B.S. Wilhelmi, C.G. Whiteley, "Response surface methodology: synthesis of short chain fructooligosaccharides with a fructosyltransferase from Aspergillus aculeatus" Bioresource Technology, vol 100, pp. 2040-2045, 2008.
[7] X.D. Wang, S.K. Rakshit, "Iso-oligosaccharides production by multiple forms of transferase enzymes from Aspergillus foetidus" Process Biochemistry, vol 35, pp. 771-775, 2000.
[8] O. Euzenat, A. Guibert, D. Combes, "Production of fructooligosaccharides by levansucrase from Bacillus subtilis C4" Process Biochemistry, vol 32, pp. 237-243, 1997.
[9] B.W. Kim, H.J. Kwon, J.P. Park, J.W. Yun, "Production of a novel transfructosylating enzyme from Bacillus maceran EG-6" Bioprocess Engineering, vol 23, pp. 11-16, 2000.
[10] D.D. Song, N.A. Jocoues, "Purification and enzymatic properties of fructosyltransferase of Streptococcus salivarius ATCC 25975" Biochemistry Journal, vol 341, pp. 285-291, 1999.
[11] J.W. Yun, D.H. Kim, H.Y. Moon, C.H. Song, S.K. Song, "Simultaneous formation of fructosyltransferase and glucosyltransferase in Aureobasidium pullulans" J. Microbiol. Biotechnol., vol 7, pp. 204-208, 1997.
[12] K. Schugerl, R. Wittler, T. Lorens, "The use of molds in pellet formations" Trends Biotechnology, vol 1, pp. 120-123, 1983.
[13] Z.N. Xu, S.T. Yang, "Production of mycophenolic acid by Penicillium brevicompactum immobilized in a rotating fibrous bed bioreactor" Enzyme and Microbial Technology, vol 40, pp. 623-628, 2007.
[14] J. Vajia, Y.Y. Linko, P. Linko, "Citric acid production with alginate bead entrapped Aspergillus niger ATCC 9142" Applied Biochemistry and Biotechnology, vol 7, pp. 51-54, 1982.
[15] T. Roukas, "Production of citric acid from beet molasses by immobilized cells of Aspergillus niger" Journal of Food Science, vol 56, pp. 878-880, 1991.
[16] A. Tay, S.T. Yang, "Production of L(+)-lactic acid from glucose and starch by immobilized cells of Rhizopus oryzae in a rotating fibrous bed bioreactor" Biotechnology and Bioengineering, vol 80 (1), pp. 1-12, 2002.
[17] P.T. Sangeetha, M.N. Ramesh, S.G. Prapulla, "Maximization of fructooligosaccharides production by two stage continuous process and its scale up" Journal of Food Engineering, vol 68, pp. 57-64, 2005.
[18] Z.J. Li, V. Shukla, K. Wneger, A. Fordyce, A.G. Pedersen, M. Marten, "Estimation of hyphal tensile strength in production-scale Aspergillus oryzae fungal fermentations" Biotechnology and Bioengineering, vol 77, pp. 601-613, 2002.
[19] N. Thongchul, "Lactic acid production by immobilized Rhizopus oryzae in a rotating fibrous bed bioreactor" Ph.D thesis, The Ohio State University, 2005.
[20] E.M. Silva, S.T. Yang, "Kinetics and stability of fibrous bed bioereactor for continuous production of lactic acid from unsupplemented acid whey" Journal of Biotechnology, vol 41, pp. 59-70, 1995.
[21] S.T. Yang, Y.M. Lo, D.B. Min, "Xanthan gum fermentation by Xanthomonas campestris immobilized in a novel centrifugal fibrous bed bioreactor" Biotechnol. Prog., vol 12, pp. 630-637, 1996.
[22] S.T. Yang, Y.M. Lo, D. Chattopadhyay, "Production of cell free xanthan fermentation broth by cell adsorption on fibers" Biotechnol. Prog., vol 14, pp. 259-264, 1998.
[23] L. Jiang, J. Wang, S. Liang, X. Wang, P. Cen, Z. Xu, "Production of butyric acid from glucose and xylose with immobilized cells of Clostridium tyrobutyricum in a fibrous bed bioreactor" Appl. Biochem. Biotechnol., vol 12, pp. 235-241, 2008.
[24] S. Rosalam, D. Krishnaiah, A. Bono, "Cell free xanthan gum production using continuous recycled packed fibrous bed bioreactor membrane" Malaysian Journal of Microbiology, vol 4(1), pp. 1-5, 2008.
[25] Y.Q. Cui, J.N.W. Ouwehand, R.G.J.M. van der Lans, M.L.F. Giuseppin, K.C.A.M. Luyben, "Aspects of the use of complex media for submerged fermentation of Aspergillus awamori" Enzyme and Microbial Technology, vol 23, pp.168-177, 1998.