Product Development and Derivatives Exploration by using Photosynthetic Bacteria

Authors: Yi-Fang Hung, Jinn-Tsyy Lai

Abstract:

Lycopene, which can be extracted from plants and is very popular for fruit intake, is restricted for healthy food development due to its high price. On the other hand, it will get great safety concerns, especially in the food or cosmetic application, if the raw material of lycopene is produced by chemical synthesis. In this project, we provide a key technology to bridge the limitation as mentioned above. Based on the abundant bioresources of BCRC (Bioresource Collection and Research Center, Taiwan), a promising lycopene output will be anticipated by the introduction of fermentation technology along with industry-related core energy. Our results showed that addition of tween 80(0.2%) and span 20 produced higher amount of lycopene. And piperidine, when was added at 48hr to the cultivation medium, could promote lycopene excretion effectively also.

Keywords: photosynthetic bacteria, lycopene, tween80, Piperidine

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1059928

References:

[1] H. Koku, I. Eroglu and U. Gunduz. "Kinetics of biological hydrogen production by the photosynthetic bacterium Rhodobacter sphaeroides", International Journal Hydrogen Energy , vol 28 (4), pp.381-388, 2003.
[2] Z. Penzkofer and S. Hegemann. "Absorption and fluorescence spectroscopic characterization of BLUF domain of App.A from Rhodobacter sphaeroides", Chemical Physiology, vol 315 (8), pp.142-154, 2005
[3] Britton, G. "Overview of carotenoid biosynthesis"., Carotenoids Biosynthesis and Metabolism, pp.143-147, 1998
[4] H.J.,Nelis, and A.P.DeLeeheer., "Microbial sources of carotenoid pigments used in food and feeds", Journal of App.lied. Bacteriology, vol 70, pp. 181-191, 1991.
[5] Deming C.., Yonbin, H., and G. Zhenxin.. "Application of statistical methodology to the optimization of fermentative medium for carotenoids production by Rhodobacter sphaeroides.", Process Biochemistry, vol 41, pp.1773-1778, 2006.
[6] T. W. Goodwin and D. G. " Studies in Carotenoid synthesis in the photosynthetic bacterium Rhodospirillum sphaeroides." Biochemistry Journal, vol 59, pp. 491-496,1955.
[7] M. L.Nguyen and S. J. Schwartz . "Lycopene: Chemical and biological properties", Food Technology, vol 53, pp.38-45, 1999.
[8] J. K. Willcox, G. L. Catignani, and Lazarus, S. "Tomatoes and cardiovascular diseases", Critical Review in Food Science and Nutrition, vol 43, pp. 1-18, 2003.
[9] Arab, L., and S. Steck. "Lycopene and cardiovascular disease" , American Journal of Clinical Nutrition, vol 71, pp.1691-1695, 2000.
[10] Assunta Z, Gianfranco C, Caterina L R., and Elena D. "Antioxidant activity of tomato lipophilic extracts and interactions between carotenoids and a-tocopherol in synthetic mixtures", LWT - Food Science and Technology,vol 43,pp. 67-72, 2010.
[11] A. V. Rao, and Agarwal, S."Role of lycopene as antioxidant carotenoid in the prevention of chronic diseases.", Nutrition Research, vol 19, pp. 305-323, 1999.
[12] Nutraingredients USA "Global carotenoid market to hit a new high", , 2007.
[13] N.S.Govind, A.R. Amin, and V.V. Modi, " Stimulation of carotenogenesis in Blakeslea trispora by cupric ions", Photochemistry, vol 21, pp.1043-1044, 1982.
[14] X. Fang, Q.Yuan. and Zhu Y. "Improved production of lycopene and β-carotene by Blakeslea trispora with oxygen-vectors", Process Biochemistry, vol 42, pp. 289-293, 2007.
[15] Sheetal M. Laxmi A, Rekha S.and Singhal. "Use of metabolic stimulators and inhibitors for enhanced production of β-carotene and lycopene by Blakeslea trispora NRRL 2895 and 2896". Bioresource Technology, vol 99, pp.3166-3173, 2008
[16] S.Kerr, J.Cal, and Cabral K." Factors enhancing lycopene production by a new Mycobacterium aurum mutant", Biotechnology Letters, vol 26, pp. 103-108, 2004.
[17] P.Bhosale, "Environmental and cultural stimulants in the production of carotenoids from microorganisms", Applied Microbiology Biotechnology, vol l63, pp. 351-361, 2004.