Commenced in January 2007
Paper Count: 30750
Aerobic Treatment of Oily Wastewater: Effect of Aeration and Sludge Concentration to Pollutant Reduction and PHB Accumulation
Abstract:This study is aimed to investigate feasibility of the aerobic biological process to treat oily wastewater from palm oil food industry. Effect of aeration and sludge concentrations are studied. Raw sludge and raw wastewater was mixed and acclimatized for five days in a stirred tank reactor. The aeration rate (no aeration, low; 1.5L/min and high rate; 2L/min) and sludge concentration (3675, 7350, and 11025mg/L of VSS) were varied. Responses of process were pH, COD, oil and grease, VSS, and PHB content. It was found that the treatment can remove 85.1 to 97.1 % of COD and remove 12.9 to 54.8% of oil & grease. The PHB yield was found to be within 0.15% to 2.4% as PHB/VSS ratio and 0.01% to 0.12% as PHB/COD removed. The higher aeration results a high COD removal and oil & grease removal, while experiment without aeration gives better PHB yield. Higher sludge concentrations (11025mg/L VSS) give higher removal of oil & grease while moderate sludge concentration (7350mg/L VSS) give better result in COD removal. Higher PHB yield is obtained in low sludge concentration (3675mg/L).
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1058641Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3889
 S. Lee and J. Yu. Production of biodegradable thermoplastics from municipal sludge by a two-stage bioprocess. Resour Conserv Recy., vol. 19, pp. 151-164, 1997
 H. Salehizadeh and M. C. M. Van Loosdrecht, "Production of polyhydroxyalkanoates by mixed culture: recent trends and biotechnological importance," Biotechnology Advances, vol. 22, pp. 261-279, 2004.
 M. C. M. Van Loosdrecht, J. J. Beun, and J. J. Heijnen, "Poly-
[beta]- hydroxyalkanoate metabolism in activated sludge," in Advances in Water and Wastewater Treatment Technology, M. Tomonori, H. Keisuke, T. Satoshi, and S. Hiroyasu, Eds., ed Amsterdam: Elsevier Science B.V., 2001, pp. 239-248.
 S. Y. Lee, K. S. Yim, H. N. Chang, and Y. K. Chang, "Construction of plasmids, estimation of plasmid stability, and use of stable plasmids for the production of poly(3-hydroxybutyric acid) by recombinant Escherichia coli," Journal of Biotechnology, vol. 32, pp. 203-211, 1994.
 H. Shimizu, S. Tamura, S. Shioya, and K.-i. Suga, "Kinetic study of poly-d(-)-3-hydroxybutyric acid (PHB) production and its molecular weight distribution control in a fed-batch culture of Alcaligenes eutrophus," Journal of Fermentation and Bioengineering, vol. 76, pp. 465-469, 1993.
 A.S.M., Chua, H. Takabatake, H. Satoh, and T. Mino. "Production of polyhydroxyalkanoates (PHA) by activated sludge treating municipal wastewater: effect of pH, sludge retention time (SRT), and acetate concentration in influent," Water Res. 37(15). Pp. 3602-3611.2003.
 N. Azbar, (2004). A Review of Waste Management Options in Olive Oil Production. Crit Rev Env Sci Tech. vol. 34, no. 3, pp 209-247
 G. Du and J. Yu, "Metabolic analysis on fatty acid utilization by Pseudomonas oleovorans: mcl-poly(3-hydroxyalkanoates) synthesis versus -oxidation," Process Biochemistry vol. 38, pp. 325-332, 2002.
 G. Du, J. Chen, J. Yu, and S. Lun, "Continuous production of poly-3- hydroxybutyrate by Ralstonia eutropha in a two-stage culture system," Journal of Biotechnology, vol. 88, pp. 59-65, 2001.
 J. Wang and J. Yu, "Kinetic analysis on inhibited growth and poly(3- hydroxybutyrate) formation of Alcaligenes eutrophus on acetate under nutrient-rich conditions," Process Biochemistry, vol. 36, pp. 201-207, 2000.
 D. Byrom, "Production of poly-
[beta]- hydroxyvalerate copolymers," FEMS Microbiology Letters, vol. 103, pp. 247-250, 1992.
 T. Tsuge, K. Tanaka, and A. Ishizaki, "Development of a novel method for feeding a mixture of L-Lactic Acid and Acetic Acid in Fed Batch Culture of Ralstonia eutropha," Journal of Bioscience and Bioengineering, vol. 91, pp. 545-550, 2001.
 Y. Poirier, "Production of new polymeric compounds in plants " Current Opinion in Biotechnology, vol. 10, pp. 181-185, 1999.
 P. Kahar, T. Tsugea, K. Taguchi, and Y. Doi, "High yield production of polyhydroxyalkanoates from soybean oil by Ralstonia eutropha and its recombinant strain," Polymer Degradation and Stability, vol. 83, pp. 79- 86, 2004.
 J. H. Law and R. A. Slepecky, "Assay of Poly-b-hydroxybutyric acid," Journal of Bacteriology, vol. 82, pp. 33-36, 1961.
 G. Braunegg, G. Lefebvre, and K. F. Genser, "Polyhydroxyalkanoates, biopolyesters from renewable resources-Physiological and engineering aspects," Journal of Biotechnology, vol. 65, pp. 127-161, 1998.
 E. Y. Lee and C. Y. Choi, "Biosynthesis and Biotechnological Production of Degradable Polyhydroxyalkanoic Acid," Biotechnol. Bioprocess Eng, vol. 2, pp. 1-10, 1997.
 B. Qu and J. Liu, "Determination of optimum operating conditions for production of polyhydroxybutyrate by activated sludge submitted to dyna
mic feeding regime," Chinese Science Bulletin, vol. 54, pp. 142- 149, 2009.
 G.-C. Du, J. Chen, H.-J. Gao, Y.-G. Chen, and S.-Y. Lun, "Effects of environmental conditions on cell growth and poly-b-hydroxybutyrate accumulation in Alcaligenes eutrophus," World Journal of Microbiology & Biotechnology, vol. 16, pp. 9-13, 2000.
 L. S. Serafim, P. C. Lemos, M. G. Albuquerque, and M. A. Reis, "Strategies for PHA production by mixed cultures and renewable waste materials," Applied microbiology and biotechnology, vol. 81, pp. 615- 28, Dec 2008.
 K. Johnson, R. Kleerebezem, and M. C. van Loosdrecht, "Influence of the C/N ratio on the performance of polyhydroxybutyrate (PHB) producing sequencing batch reactors at short SRTs," Water research, vol. 44, pp. 2141-52, Apr 2010.
 S. Shahhosseini, "Simulation and optimisation of PHB production in fed-batch culture of Ralstonia eutropha," Process Biochemistry, vol. 39, pp. 963-969, 2004.